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Hazard assessment for the terrestrial environment
Book of Abstmcts - EUROTOX ‘94
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protein-A (SPA] has multipotent immunomodulatory and antitumor activity [2]. The present study deals with the effeet of SpA on adrenal gland and testis. SpA was administered intraperitoneally,twice a week for 2 and 8 weeks at the doses of 1 pg and 6 rg in 200 ~1 normai saline. The significantchanges were found in relative organ weights of both adrenal gland and testis. In adrenal cortex, a notable hypertrophyof zona glumerulosa and its parenchymal cells and marked zona fasciculata were observed with 1 fig SpA at 2 weeks. The adrenal masses were observed outside the capsule of the adrenal. In testis, SpA exhibited biphasic effects, at one end it inhibited spermatogenesis and other end stimulated spermatogonial proliferation. Prolong administration with high doses of SpA have produced toxic lesions in mice. [l] Ribelin, W.E., Fundam. Appl. Toxicol.36,105 (1984). [2] Mishra, A., Dwivedi, PD., Verma, AS., Ray PK., Immunol. Lett. 34,289 (1992). Key wards: adrenal gland; Staphylococcus protein-A; testis
Hazard Assessmentfor the TerrestrialEnvironment L. Samsee-Petersen, E Pedersen. Vrul,Danish Water Duality Institute, Hersholm, Denmark Within the OECD Chemicals Programme priority has still recently been given to the development and harrnonisation of strategies for the assessment of hazard and risk of chemicals in the aquatic environment, resulting in a Guidance Document for Aquatic Effects Assessment. For the development of similar strategies for the terrestrial environment the OECD Hazard Assessment Advisory Body (HAAB! %asinitiated the preparationof a discussiondocument to identify the variousissues and needs for the OECD Hazard Asssssment Programme. The main topics covered by this discussiondocument are brieflyreviewed highlightingsubjects forwhich research and/or decisions are needed - and with special emphasis on effects assessment. The main routes of emission of chemicals to the terrestrial environment are described. The complexity of the terrestrial environment and its compartments are reviewed in order to identify groups of organismsof concern. Elements to be considered before the choice of terrestrial toxici*/ test methods for guideline development are discussed. Finallypotential strategies for hazard and risk assessment for the terrestrial environment are identified, and a suggestion for the calculationof PNEC E r soil is presented. i(ev words: terrestrial environment; effects assessment: hazard assessment
Per&tant Level of HCH in L;ibeo
Rohita
arcdIts Impacton HumanHealth
V SankaraRao.Y.K. Khillare. Depattment of Zootom 0~ Babasaheb Ambedkar Martathwada tJc.&?kzi& Aurangabad 43 1004, India For the in vitro analysis. L. robit? were collected from polluted water reservoir located at Guntur Dist. AR state, India. for the assessment of persistant level of HCH in the various tissues through GC 6 TLC application. Analysis was compared with other fishes collected from uncontaminated water bodies. Persistant level in the fish found very significant, and noticed that, original HcH was converted into its isomeric forms like, a, /.I and y. Highest level of a HCH observed in gonad, 5760 rg/ksfollowed by liver, 1560 &g/kg and in muscle, 460 fig/kg. Similarly,B HCH 4350 fig/kg in gonad, 2720 pglkg in liver and 126 #g/kg in muscle. y I-tCH in gonad 2560 @g/kg, in liver 365 fig/kg and 263 pg/kg in muscle. On the above data it can be said that, there is more persistency of HCH in gonad than any other tissues. Each of the isomer of HCH proven to be toxic in animal including fish, and hence transfer toxicity to human health on consuming. Moreover, has more biomagnificatior, capacity which strengthens on chronic exposure. Accumulation of a compound in the tissue depends upon it’s nature. Key words: HCH isomers; L. ;nhita; GC 6 TLC application and tissues
The Dispositionand Metabolismof 4-Ethyltoluenein Rats A. Sapota. D. Liaocka. Jnstitute of Occupational Medicine, Lfddf Poland +Ethyltoluene (ET) is a main component of Farbasol,a mixture of solvents used among others in paints products.The present study was designed to investigate distribution,excretion, and metabolism of ET(ring@H) after a single i.p. administration of the compound to male Wistar albino rats. The concentration of tritium 3H in blood plasma showed a multi-phase decline with a distribution phase (TIN -1.5 h) followed by two elimination phases (TX J t2 13 h]. Considering the major tissues the highest retention of 3H. 72 hours after administrationwas detacted in fat, sciatic
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protein-A (SPA] has multipotent immunomodulatory and antitumor activity [2]. The present study deals with the effeet of SpA on adrenal gland and testis. SpA was administered intraperitoneally,twice a week for 2 and 8 weeks at the doses of 1 pg and 6 rg in 200 ~1 normai saline. The significantchanges were found in relative organ weights of both adrenal gland and testis. In adrenal cortex, a notable hypertrophyof zona glumerulosa and its parenchymal cells and marked zona fasciculata were observed with 1 fig SpA at 2 weeks. The adrenal masses were observed outside the capsule of the adrenal. In testis, SpA exhibited biphasic effects, at one end it inhibited spermatogenesis and other end stimulated spermatogonial proliferation. Prolong administration with high doses of SpA have produced toxic lesions in mice. [l] Ribelin, W.E., Fundam. Appl. Toxicol.36,105 (1984). [2] Mishra, A., Dwivedi, PD., Verma, AS., Ray PK., Immunol. Lett. 34,289 (1992). Key wards: adrenal gland; Staphylococcus protein-A; testis
Hazard Assessmentfor the TerrestrialEnvironment L. Samsee-Petersen, E Pedersen. Vrul,Danish Water Duality Institute, Hersholm, Denmark Within the OECD Chemicals Programme priority has still recently been given to the development and harrnonisation of strategies for the assessment of hazard and risk of chemicals in the aquatic environment, resulting in a Guidance Document for Aquatic Effects Assessment. For the development of similar strategies for the terrestrial environment the OECD Hazard Assessment Advisory Body (HAAB! %asinitiated the preparationof a discussiondocument to identify the variousissues and needs for the OECD Hazard Asssssment Programme. The main topics covered by this discussiondocument are brieflyreviewed highlightingsubjects forwhich research and/or decisions are needed - and with special emphasis on effects assessment. The main routes of emission of chemicals to the terrestrial environment are described. The complexity of the terrestrial environment and its compartments are reviewed in order to identify groups of organismsof concern. Elements to be considered before the choice of terrestrial toxici*/ test methods for guideline development are discussed. Finallypotential strategies for hazard and risk assessment for the terrestrial environment are identified, and a suggestion for the calculationof PNEC E r soil is presented. i(ev words: terrestrial environment; effects assessment: hazard assessment
Per&tant Level of HCH in L;ibeo
Rohita
arcdIts Impacton HumanHealth
V SankaraRao.Y.K. Khillare. Depattment of Zootom 0~ Babasaheb Ambedkar Martathwada tJc.&?kzi& Aurangabad 43 1004, India For the in vitro analysis. L. robit? were collected from polluted water reservoir located at Guntur Dist. AR state, India. for the assessment of persistant level of HCH in the various tissues through GC 6 TLC application. Analysis was compared with other fishes collected from uncontaminated water bodies. Persistant level in the fish found very significant, and noticed that, original HcH was converted into its isomeric forms like, a, /.I and y. Highest level of a HCH observed in gonad, 5760 rg/ksfollowed by liver, 1560 &g/kg and in muscle, 460 fig/kg. Similarly,B HCH 4350 fig/kg in gonad, 2720 pglkg in liver and 126 #g/kg in muscle. y I-tCH in gonad 2560 @g/kg, in liver 365 fig/kg and 263 pg/kg in muscle. On the above data it can be said that, there is more persistency of HCH in gonad than any other tissues. Each of the isomer of HCH proven to be toxic in animal including fish, and hence transfer toxicity to human health on consuming. Moreover, has more biomagnificatior, capacity which strengthens on chronic exposure. Accumulation of a compound in the tissue depends upon it’s nature. Key words: HCH isomers; L. ;nhita; GC 6 TLC application and tissues
The Dispositionand Metabolismof 4-Ethyltoluenein Rats A. Sapota. D. Liaocka. Jnstitute of Occupational Medicine, Lfddf Poland +Ethyltoluene (ET) is a main component of Farbasol,a mixture of solvents used among others in paints products.The present study was designed to investigate distribution,excretion, and metabolism of ET(ring@H) after a single i.p. administration of the compound to male Wistar albino rats. The concentration of tritium 3H in blood plasma showed a multi-phase decline with a distribution phase (TIN -1.5 h) followed by two elimination phases (TX J t2 13 h]. Considering the major tissues the highest retention of 3H. 72 hours after administrationwas detacted in fat, sciatic