Head and neck dermal neurofibroma contains distinctive mesenchymal-like tumour stem cells governed by neurotrophin microenvironment

354 Accordingly, the importance of consultative liaison clinic has been confirmed. http://dx.doi.org/10.1016/j.ijom.2017.02.1190 Application of smart watch in navigation system for oral implant surgery S. Usuda ∗ , K. Yu, K. Ohnishi, T. Nakagawa, H. Kawana Tachikawa Hospital, Tokyo, Japan Background: Complications of the oral implant surgery have been induced problems like nerve palsy and vascular injury. Objectives: To solve these problems, surgical navigation systems have being developed. The surgeon can get the position information during surgery form navigation monitor installed outside. However, it is an inefficient method because that surgeon must look the monitor many times and it makes surgeon tired. In this study, we utilised a smart watch as an efficient information transfer method. Methods: This system consists of a smart watch (or, smart phone, tablet computer) that displays the distance to the target position graphically, and a three-axis robot manipulator, which measures the potion. 20 times of experiment was conducted to evaluate the time and accuracy using each device. Findings and Conclusion: The experimental results show that there were no differences in accuracy between smart watch, smart phone, and tablet computer. However, there were significant differences (P < 0.01) that smart watch requires more time than other two devices. The difference was caused by the slow update rate (30 ms) of the smart watch that does not use Wi-Fi but use Bluetooth connection. On the other hand, the impression from use of smart watch was better than other two devices because it has closer focal distance and it appears to be a superior method for the information transfer. On the future work, the improvement of the update rate and the GUI system will be conducted. http://dx.doi.org/10.1016/j.ijom.2017.02.1191 Head and neck dermal neurofibroma contains distinctive mesenchymal-like tumour stem cells governed by neurotrophin microenvironment L. Wang ∗ , Z.H. Yang, D.L. Lei, C.P. Zhang Ninth People’s Hospital, Shanghai Jiao Tong University, Shanghai, China Background: Dermal neurofibroma is a challenging genetic disease resulting in severe facial disfigurement, without satisfactory treatments. Therefore, it is urgently needed to develop a cure based on novel stem cell-level mechanisms of neurofibroma. Objectives: To determine whether dermal neurofibroma contains tumour stem cells and their potential differences derived from head and neck versus trunk, and to clarify their stem cell microenvironment. Methods: Immunohistochemistry was used to evaluate the proportion of CD44, CD146 or Nestin positive cells in neurofibroma tissues. Head and neck dermal neurofibroma tissues were harvested from patients for isolation and culture of tumour stem cells. Tumour stem cells were characterised by flow cytometry, osteogenic/adipogenic induction and subcutaneous implantation

to immunocompromised mice. Non-head and neck neurofibroma and normal facial skin were used as controls. Findings: Head and neck dermal neurofibroma showed abundant CD44, CD146 or Nestin positive cells. Colony-forming tumour stem cells were successfully isolated and cultured from dermal neurofibroma. Stem cells derived from head and neck neurofibroma showed the highest capabilities of multilineaged differentiation and forming neurofibroma-like tissues. NF mesenchymal-like stem cells generate a tumour microenvironment via autocrine loop of BDNF/TrkB. Conclusion: A new subgroup of NF mesenchymal-like tumour stem cells was discovered and characterised. Different origins of NF stem cells generate different levels of BDNF microenvironment, thus determining different tumour phenotypes. These distinctive tumour stem cells in head and neck neurofibroma are very likely to be a novel target for treating this challenging disease. http://dx.doi.org/10.1016/j.ijom.2017.02.1192 The role of melanoma differentiation-associated gene-7/interleukin-24 in epithelial differentiation L. Wang ∗ , Z. Han, H.H. Wu, Y.X. Wang, C.B. Guo Peking University School and Hospital of Stomatology, China Background: Epithelial differentiation is one of the key factors during epithelial homeostasis. The Disruption of this process was strongly related to many diseases, including tumourigenesis. In our previous study, we have demonstrated an association of melanoma differentiation-associated gene-7 (MDA-7)/interleukin-24 (IL24) expression with the pathologic grade of 131 head and neck squamous cell carcinoma (HNSCC) specimens. However, its epithelial differentiation regulatory role still remains unclear. Objectives: To investigate the role of MDA-7/IL-24 expression in epithelial differentiation. Methods: Three-dimensional epithelial culture was constructed using a human immortalised epithelial cell line HaCaT. IL20R2 gene was transfected into WSU-HN6 cells to obtain a new stable expression cell line HN6-IL20R2. Epithelial differentiation markers and key factors in signalling pathway were detected by reverse transcriptase-polymerase chain reaction, western blot in above two cell lines, in order to evaluate the epithelial differentiation status and investigate the mechanism. Results and Conclusion: Recombinant human IL-24 (rhIL24) treated three-dimensional epithelial culture has shown more layers of epithelium and arrangement disorders. Higher differentiation was observed in HaCaT cells compared to transfected cell line HN6-IL20R2. Down-regulation of epithelial terminal differentiation markers (loricrin and filaggrin) and up-regulation of early epithelial differentiation markers (integrin ␤4) were found in rhIL24 treated HaCaT cells. However, in HN6-IL20R2 cell, opposite results were detected. rhIL24 could down-regulate Notch1 expression in HaCaT, but in HN6-IL20R2 cells, we proved contrast founding. By conclusion, our results suggested that MDA7/IL-24 was an important regulator of epithelial differentiation and maintenance during epithelial homeostasis. Furthermore, Notch1 could be involved in this process. http://dx.doi.org/10.1016/j.ijom.2017.02.1193