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Hepatic monoamine oxidase activity after chronic ethanol intoxication in the rat
tOP:PARISON OF FtRST AND SECOND GENERATION TESTS FOR HEPATITIS t VIRUS %nho. f.Z3maIho, J.Velosa. F.SerejO, kK %ura LIYW unrt. vedicina 2. kdical School of Lisbon. TO compare the 1st and 2nd qeneraticn tests for hepat~tls C virus in chronic llwr diseases,we tested 116 ~~a.62 pt5 Yith chronic NANB.5 auto-urnmune hepatlt~slkl),dDHCC and 9 alcoholic cirrhusl5
(ACi.This last 2 groups were all we to anti-HCY in the lit generation test.ThIs test u5e the nonstructural antigen(ciDD-1) and the 2nd generation the nowstructural(c200) and the structural (~22-3) of HCV,(ELISA.DrthoDiagnostic Systems).ReWits: I Of pritiwl+vel cases 1st generation 2"d aeneration 90%(56/62) HAMB Hepatitis RB%(55/62) ns 42%(17/4D) 4rn[l6/401 ns HFC 1wI lW9l p&D5 551 W9) AC 60% w51 AI IDDZ 1'5) ns _. 9% of Dts wth chronic NAW hepatitis&we anti-HCV
antigen
po!.,t~ve with both tests.OUr data suggest that the 2nd generation test 5eems to have a higher speciflcity than the 1st one newly I" AI HepatltlS.The prevalence of anti-HCV.with simultaneous testS,SS new 100% in chronic tiANB hwatitic.This luggest the avallabllity of a test containing the 3 anti-
gens.
The polymzase chain reaction is a nnre sensitlre method available for the determination of ~NPI-WR. To coupare the utility of the determnb tmn of DNA-IMB by PCR and by dot-blot hybridiza tion, in the follow-up of the CHR antlHBe psitive. MWVDDS:Forty-sevenserumsamples be141ging to 13 iZ%XiFantiHBe positive patlen& (p.1 were Obtained in the Jan-Dee IWO pericd.fn this sexples thepam ce OP MJA-IM!?. was studied by dot-biot hibrldlzatlon
_ .._
OBTmIvE:
and by Pea using primers oE the precore-core req~on A livsc biopsy wau~5 previously obtained in 10 p. ~?S:mirty-twa sjmples have c.xncukntal tesuks (~po-_itive, 2"negarivesl and 15 were positives by IG? ind "egatlves by dot-blot ,p‘"."Dl). Pa-
tients were dr:anged according to the PCRresults: PermanentlyPCR+: 4p.. 16 samples (15 pcR+, 5 dot blot +), 4 biopsies (3 CAR/l CWI. Inrecm~trent ly FCR+: 4p.. 18 samples (hPCR*, 2 dot - blot +I3 blopsles I1 LC/2 CM). PC~ -:5 p., 13 samples (none PCRnor dot blot +I. 3 biopsies 13cRn/2 cpnl Intermittently ON?+WBwitlve p. by PCRexhlblt pbsitive results whenALT exacerbatrons occur. I33KLusIONs:l.- DNA-VHB determlnatlnn by Pa? is USeFUlto evaluate viral repllcatian in the HCB antinse positive patients. 2.- Petlents with exacerbations in his evolution seems to present mxe severe histological lessons.
5143
(ACi.This last 2 groups were all we to anti-HCY in the lit generation test.ThIs test u5e the nonstructural antigen(ciDD-1) and the 2nd generation the nowstructural(c200) and the structural (~22-3) of HCV,(ELISA.DrthoDiagnostic Systems).ReWits: I Of pritiwl+vel cases 1st generation 2"d aeneration 90%(56/62) HAMB Hepatitis RB%(55/62) ns 42%(17/4D) 4rn[l6/401 ns HFC 1wI lW9l p&D5 551 W9) AC 60% w51 AI IDDZ 1'5) ns _. 9% of Dts wth chronic NAW hepatitis&we anti-HCV
antigen
po!.,t~ve with both tests.OUr data suggest that the 2nd generation test 5eems to have a higher speciflcity than the 1st one newly I" AI HepatltlS.The prevalence of anti-HCV.with simultaneous testS,SS new 100% in chronic tiANB hwatitic.This luggest the avallabllity of a test containing the 3 anti-
gens.
The polymzase chain reaction is a nnre sensitlre method available for the determination of ~NPI-WR. To coupare the utility of the determnb tmn of DNA-IMB by PCR and by dot-blot hybridiza tion, in the follow-up of the CHR antlHBe psitive. MWVDDS:Forty-sevenserumsamples be141ging to 13 iZ%XiFantiHBe positive patlen& (p.1 were Obtained in the Jan-Dee IWO pericd.fn this sexples thepam ce OP MJA-IM!?. was studied by dot-biot hibrldlzatlon
_ .._
OBTmIvE:
and by Pea using primers oE the precore-core req~on A livsc biopsy wau~5 previously obtained in 10 p. ~?S:mirty-twa sjmples have c.xncukntal tesuks (~po-_itive, 2"negarivesl and 15 were positives by IG? ind "egatlves by dot-blot ,p‘"."Dl). Pa-
tients were dr:anged according to the PCRresults: PermanentlyPCR+: 4p.. 16 samples (15 pcR+, 5 dot blot +), 4 biopsies (3 CAR/l CWI. Inrecm~trent ly FCR+: 4p.. 18 samples (hPCR*, 2 dot - blot +I3 blopsles I1 LC/2 CM). PC~ -:5 p., 13 samples (none PCRnor dot blot +I. 3 biopsies 13cRn/2 cpnl Intermittently ON?+WBwitlve p. by PCRexhlblt pbsitive results whenALT exacerbatrons occur. I33KLusIONs:l.- DNA-VHB determlnatlnn by Pa? is USeFUlto evaluate viral repllcatian in the HCB antinse positive patients. 2.- Petlents with exacerbations in his evolution seems to present mxe severe histological lessons.
5143