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Hepatitis C: Pathogenesis
90A
AASLD PROGRAM
II......
Tuesday Posters
H E P A T I T I S C: P A T H O G E N E S I S
#893
Specific inhibition of hepatitis C virus core antigen production by antisense RNA constructs. T Wakita, D Moradpour, K Tokushige and JR Wands. Molecular Hepatol. Lab., Massachusetts Gen. Hosp. Cancer Ctr. and Harvard Med. Sch,, Charlestown, MA.
#894
Specific inhibition of hepatitis C virus gene expression by antisense oligonueleotides in a tightly regulated cell culture system.
#900 Efficient production of a portion of the hepatitis C core protein and analysis of the murine i m m u n e response to recombinant protein: toward a vaccine for hepatitis C. Y Hitomi, WM McDonnell, JR Baker, Jr and FK Askari. Dept. of Internal Med., Div. of Gastro. and Allergy & Immunol., Univ. of Michigan Med. Ctr. and Veterans Admin. Med. Ctr., Ann Arbor, MI.
#901
D Moradpour, T Wakita, C Englert and JR Wands. Molecular Hepatol. Lab. and Lab. of Molecular Genetics, Massachusetts Gen. Hosp. Cancer Ctr., Harvard Med. Sch., Charlestown, MA.
DNA. CH Wu, A Siddiqui and GY Wu. Dept. of Med., Div. of Gastro.,, Univ. of Connecticut Sch. of Med., Farmington, CT; Dept. of Microbiol., Univ. of Colorado HSC, Denver, CO.
#902 Maturation and biological importance of hepatitis C virus core protein. K Yasui, T Wakita, KT Kohara, M Ichikawa, T Kajita, A Hasegawa and M Kohara. The Tokyo Metro. Inst. of Med. Science, Tokyo, 3rd Dept. of Internal Med., Kyoto Prefectural Univ. of Med., Kyoto, The Tokyo Metro. Inst. for Neuroscience, Internatl. Reagents Co. and Corp. Research & Development Lab., Tonen Co., Tokyo, Japan.
Inhibition of hepatitis C virusdirected translation by hammerhead ribozymes in vitro. N Sakamoto, CH Wu and GY Wu. Dept. of Med., Div. of Gastro.-Hepatol., Sch. of Med., Univ. of Connecticut Health Ctr., Farmington, CT.
#897 Expression of enzymatically active HCV NS3 protease (genotype lb) and the development of an in vitro protease assay system. JA Feller, AR Carroll, DV Sangar, JYN Lau and BE Clarke. Section of Hepatobiliary Diseases, Dept. of Med., Univ. of Florida, Gainesville, FL; Wellcome Research Labs, Beckenham, England.
HCV core protein subcellular localization: analysis by confocal and electron microscopy and correlation with the cell cycle. G Barba, T Harada, F Harper, Y Matsuura, T Miyamura and C Br~chot. Liver Cancer and Molecular Virol., INSERM U370, Paris, France; Dept. ofVirol. II, Natl. Inst. of Health, Tokyo, Japan; Lab. de Biol. et Ultrastructure du Noyau, CNRS UPR272, Villejuif, France.
#895 Targeted inhibition of HCV promoter activity using HCV-specific antisense
#896
HEPATOLOGYOctober 1995
#903
Single-strand conformation p o l y m o r p h i s m analysis of the HCV h y p e r v a r i a b l e region. RR Lareu, WD Reed and NR Swanson. Univ. Dept. Med. and Dept. Gastro., QEIIMC, Nedlands, Australia.
#904 Increased variability in the HCV RNA hypervariable region among patients with HCV/HIV co-infection.
#898 Antibody response to hypervariable region of HCV differs between genotype lb and 2a.
KE Sherman, C Andreatta, J O'Brien, A Gutierrez and R Harris. Dept. of Med. and Pathol., Univ. of Cincinnati Med. Ctr., Cincinnati, OH; Fitzsimons Army Med. Ctr., Aurora, CO.
K Yoshioka, T Aiyama, A Okumura, M Takayanagi, K Iwata, T Ishikawa and S Kakumu. 3rd Dept. of Internal Med., Nagoya Univ. Sch. of Med., Nagoya, Japan.
#905 In vitro effects of interferon alpha-2B on hepatitis C virus replication in lymphocytes.
#899 Expression and characterization of the NS5B (RNA-dependent RNA polymerase) gene of hepatitis C virus. RH Al, Y Wang, Y xie and CH Hagedorn. Div. of Digestive Diseases, Emory Univ. Sch. of Med. and VA Med. Ctr., Atlanta, GA.
X Wang and AH Sherker. Lady Davis Inst. for Med. Research, SMBD-Jewish Gen. Hosp., McGill Univ., Montreal, Canada.
#906 Molecular mapping of an intrahepatic hepatitis C virus-specific crossgenotype cytotoxic T lymphocyte epitope. CG Marousis, DR Nelson and JYN Lau. Section of Hepatobiliary Diseases, Univ. of Florida, Gainesville, FL.
HEPATOLOGYVol. 22, No. 4, Pt. 2, 1995
AASLD PROGRAM
91A
Tuesday Posters #907
The p e r i p h e r a l b l o o d c y t o t o x i c T l y m p h o c y t e r e s p o n s e to HCV does n o t r e f l e c t d i s e a s e activity during c h r o n i c HCV infection. B Rehermann, KM Chang, JG McHutchison, RP Kokka, M Houghton and FV Chisari. Scripps Research Inst., La Jolla and Chiron Corp., Emeryville, CA.
#913
CD30-induction on T l y m p h o c y t e s by HCV-derived a n t i g e n s in p a t i e n t s w i t h c h r o n i c h e p a t i t i s C. M Lechmann, B Irrgang, HG Ihlenfeldt, G Jung, T Sauerbruch and U Spengler. Dept. of Gen. Med., Univ. of Bonn and Inst. of Organic Chem., Univ. of Tuebingen, Germany.
#908
Viral e s c a p e from the HI,A-A2 r e stricted cytotoxic T lymphocyte r e s p o n s e is n o t c o m m o n during c h r o n i c h e p a t i t i s C virus infection. KM Chang, B Rehermann, JG McHutchison, C Pasquinelli and FV Chisari. Scripps Research Inst., La Jolla, CA.
#914
C y t o k i n e expression and T-cell subset activation in chronic hepatitis C. RP Woitas, M Sippel, A Blaufuss, T Sauerbruch and U Spengler. Dept. of Gen. Med., Univ. of Bonn, Germany.
#915
T h l c y t o k i n e p a t t e r n and d e f e c t i v e IL-10 in c h r o n i c h e p a t i t i s C virusinfected livers. AS Gaweco, DH Van Thiel, V Rustgi, T Hassanein, L E1-Ashmawy, T Whiteside, WJ Hofmann and HF Otto. Inst. of Pathol., Univ. of Heidelberg, Germany; Oklahoma Transplant Inst., Oklahoma City, OK; INOVA Inst., Fairfax, VA; Dept. of Pathot., Univ. of Pittsburgh, PA.
#916
Immunodominant hepatitis C virus core epitopes s t i m u l a t e CD3÷/CD30 + l y m p h o c y t e s for p r o d u c t i o n o f TH0like c y t o k i n e s i n p a t i e n t s w i t h chronic h e p a t i t i s C. RP Woitas, M Lechmann, HG Ihlenfeldt, G Jung, T Sauerbruch and U Spengler. Depts. of Gen. Med., Univ. of Bonn and Inst. of Organic Chem., Univ. of Tuebingen, Germany.
#917
D e f e c t i v e a n t i v i r a l c y t o k i n e s in chronic h e p a t i t i s C v i r u s - i n f e c t e d livers. AS Gaweco, V Rustgi, T Hassanein, L E1-Ashmawy, T Whiteside, WJ Hofmann, DH Van Thiel and HF Otto. Inst. of Pathol., Univ. of Heidelberg, Germany; INOVA Inst., Fairfax, VA; Oklahoma Transplant Inst., Oklahoma City, OK; Dept. of Pathol., Univ. of Pittsburgh, PA.
#918
Quantitative analysis o f i n t e r f e r o n a/~ r e c e p t o r m R N A in the liver o f patients w i t h c h r o n i c h e p a t i t i s C. H Yatsuhashi, MC Parquet, K Yamasaki, F Iio, T Aritomi, M Yamasaki, O Inoue, M Koga and M Yano. Inst. for Clinical Research, Nagasaki Chuo Natl. Hosp., Nagasaki, Japan.
#919
Changes in s e r u m soluble interleukin-2 r e c e p t o r l e v e l s in p a t i e n t s w i t h chronic h e p a t i t i s C b y interferon-a. T Takeda, S Nishiguchi, S Nakajima, S Shiomi, T Kuroki and K Kobayashi. 3rd Dept. of Internal Med., Osaka City Univ. Med. Sch., Osaka, Japan.
#909
C o m p a r a t i v e analysis o f the cytotoxic T cell r e s p o n s e in c h r o n i c HBV and HCV infection. B Rehermann, KM Chang, JG McHutchison and FV Chisari. Scripps Research Inst., La Jolla, CA,
#910
H e p a t i t i s C virus-specific cytotoxic T l y m p h o c y t e s f r o m liver a n d peripheral blood of a patient with a s u s t a i n e d r e s p o n s e to i n t e r f e r o n therapy. DKH Wong, MJ Koziel, D Dudley, JT Wang, N Afdahl, C Rice, M Houghton and BD Walker. Infectious Disease Unit, Massachusetts Gen. Hosp. and Boston City Hosp., Boston, MA; Washington Univ., St. Louis, MO; Chiron Corp., Emeryville, CA.
#911
#912
A c c u m u l a t i o n o f HCV-core specific T cell c l o n o t y p e s in patients w i t h c h r o n i c h e p a t i t i s C and its r e d u c t i o n f o l l o w i n g e r a d i c a t i o n o f HCV-RNA by antiviral treatment. A Tanaka, H Kita, M Takatori, S Iwabuchi, K Kurokawa, M Imawari, K Nishioka, S Iino and K Yamamoto. Inst. ofMed. Sci. and 2nd Dept. of Internal Med., St. Marianna Univ., Sch. ofMed., Kanagawa, Liver Study Lab., Jichi Univ., Sch. ofMed., Tochigi and 1st and 3rd Dept. of Internal Med., Univ. of Tokyo, Japan. Viral c l e a r a n c e d u r i n g a c u t e h e p a t i t i s C is associated w i t h a NS3-specific CD4 + T cell response: functional and m o l e c u l a r analysis at the clonal level. HM Diepolder, R Zachoval, RM Hoffmann, EA Wierenga, T Santantonio, M-C Jung, M W~ichtler, D Eichenlaub and GR Pape. Inst. for Immunol. and Dept. of Med. II, Klinikum Grosshadern, Univ. of Munich, Germany.
92A
AASLD PROGRAM
HEPATOLOGYOctober 1995
Tuesday Posters #920
T r a n s f o r m i n g g r o w t h factor-~l in c h r o n i c h e p a t i t i s C.
#927
DR Nelson, CG Marousis, KP Qian, Y Xu, RP Gonzalez-Peralta, GL Davis and JYN Lau. Section of Hepatobiliary Diseases, Univ. of Florida, Gainesville, FL.
#921
P a t t e r n s of i m m u n e r e s p o n s e s to the h o s t - e n c o d e d GOR a n d h e p a t i t i s C virus core-derived epitopes with relation to hepatitis C viremia and d i s e a s e severity.
K Fujii, K Hino, M Okazaki, M Korenaga, C Murakami, M Okuda, M Ikeda, S Kondoh and K Okita. Dept. of Internal Med., Yamaguchi Univ. Sch. of Med., Yamaguchi, Japan.
#928
JA Quiroga, M Pardo, S Navas, J Martin and V Carrefio. Hepatol. Unit, Fundacion Jimenez Diaz, Madrid, Spain.
#922
#923
#924
E x p r e s s i o n of CD80 (B7/BB-1) in l i v e r t i s s u e s of p a t i e n t s w i t h c h r o n i c h e p a t i t i s C. K Mochizuki, N Hayashi, K Katayama, N Hiramatsu, Y Kawanishi, A Kasahara, H Fusamoto, T Yokochi and T Kamada. 1st Dept. of Med., Osaka Univ. Sch. of Med., Suita and Dept. of Bacteriol., Aichi Med. Univ. Sch., Aichi, Japan.
P h e n o t y p i c a n d f u n c t i o n a l charact e r i z a t i o n of i n t r a h e p a t i c n a t u r a l killer a n d m a c r o p h a g e s i n viral chronic hepatitis.
#925
#926
#929
R e l e v a n c e of u l t r a s t r u c t u r a l l y m p h o c y t e e m p e r i p o l e s i s in h e p a t o c y t e s in c h r o n i c h e p a t i t i s C.
#931
K Fukuda, S Nishiguchi, T Kuroki and K Kobayashi. 3rd Dept. of Internal Med., Osaka City Univ. Med. Sch., Osaka, Japan.
D-related h u m a n l e u k o c y t e a n t i g e n s a n d d i s e a s e s e v e r i t y in c h r o n i c h e p a t i t i s C. AJ Czaja, HA Carpenter, PJ Santrach and SB Moore. Mayo Clinic, Rochester, MN.
Virological c h a r a c t e r i z a t i o n of hepatitis C v i r u s h e a l t h y carriers. M Debette, J Coste, V Hyrailles, P Blanc, A Dubois, H Michel and D Larrey. Serv. d'Hepatogastro., Hop. Saint-Eloi and Ctr. de Transfusion Sanguine, Montpellier, France.
#932
HCV infection of peripheral blood m o n o n u c l e a r cells assessed b y in s i t u reverse t r a n s c r i p t a s e - p o l y m e r a s e c h a i n reaction: a pathogenetic role in HCV-related m i x e d cryoglobulinemia?
H e p a t i t i s C v i r u s r e p l i c a t i o n in p e r i p h e r a l b l o o d m o n o n u c l e a r cells i n r e l a t i o n to r e s p o n s e to i n t e r f e r o n therapy.
L o w e x p r e s s i o n of e r y t h r o c y t e c o m p l e m e n t r e c e p t o r type I i n d u c e s defective i m m u n e c o m p l e x c l e a r a n c e in c h r o n i c h e p a t i t i s C p a t i e n t s . T Kanto, N Hayashi, T Takehara, K Katayama, A Kasahara, H Fusamoto, M Kato, M Akiyama and T Kamada. 1st Dept. of Med., Osaka Univ. Med. Sch., Suita, Dept. of Gastro., Osaka Natl. Hosp., Osaka and Dept. of Med., Shin-Senri H0sp., Suita, Japan.
#930
L Muratori, M Lenzi, D Gibellini, M Frisoni, M Cataleta, P Muratori and FB Bianchi. Depts. of Internal Med., Inst. of Microbiol. and Rheumatol., Univ. of Bologna, S. Orsola-Malpighi, Bologna, Italy.
Immunological events during the i n c u b a t i o n p e r i o d of h e p a t i t i s C v i r u s i n f e c t i o n in i m m u n o c o m p r o m i s e d individuals. M Psichogiou, A Katsoulidou, E Vaindirli, B Francis, SR Lee and A Hatzakis. Athens Univ. Med. Sch., Athens, Greece; Ortho Diagnostic Systems Inc., Raritan, NJ.
C Garc~a-MonzSn, L Garc~a-Buey, MJ Borque, A Garcia-S~nchez, JM Pajares and R Moreno-Otero. Liver Unit, Molecular Biol. Unit and Pathol. Serv., Hosp. de la Princesa, Univ. Autonoma de Madrid, Spain.
J Gabanyi, D Vetter, M Doffoel, A Ananna, G Uhl and JM Vetter. Serv. d'Hepatogastro. and Inst. d'Anatomie Pathol., Hop. Civil, Strasbourg, France.
Different q u a s i s p e c i e s of h e p a t i t i s C v i r u s RNA b e t w e e n s e r u m a n d perip h e r a l b l o o d m o n o n u c l e a r cells.
Chronic hepatitis C virus infection in HIV p o s i t i v e patients: i n f l u e n c e of h o s t i m m u n i t y . L Serfaty, C Housset, L Morand-Joubert, J Frottier, J Lebas, PP De Saint-Maur and R Poupon. Unite d'Hepatol., Serv. de Bacteriol.-Virol., Maladies Infectieuses, Med. Interne and Anatomopathol., Hop. St.-Antoine, Paris, France.
#933
C h r o n i c h e p a t i t i s C of d r u g users: i n f l u e n c e of HIV i n f e c t i o n . S Pol, N Trinh Thi, V Thiers, F Jaffredo, F Carnot, B Lamorthe, H Zylberberg, P Berthelo~, C Br~chot and B Nalpas. Liver Unit, INSERM U-370, Hop. Necker and Hybridotest, Inst. Pasteur, Paris, France.
HEPATOLOGYVol. 22, No. 4, Pt. 2, 1995
AASLD PROGRAM
93A
Tuesday Posters #934
Hepatitis C v i r u s density heterogeneity a n d v i r a l t i t r e in acute a n d c h r o n i c infection: a comparison of immunodeficient a n d i m m u n o c o m p e t e n t patients. JP Watson, DJ Bevitt, GP Spickett, GL Toms and MF Bassendine. Depts. ofMed., Virol. and Immunol., Univ. of Newcastle-uponTyne, England.
#935
#937
S p o n t a n e o u s f l u c t u a t i o n s of viral l o a d in u n t r e a t e d p a t i e n t s w i t h c h r o n i c h e p a t i t i s C. M Bourli~re, P Halfon, P Berthezene, H Khiri, I Portal, C Wartelle, V Gerolami, G Cartouzou and AP Gauthier. Liver Unit, Hop. Saint Joseph, Hop. de la Conception, Alpha Bio. and INSERM U315, Marseille, France.
#939
#940
#943
Wide f l u c t u a t i o n of p l a s m a t i c HCV g e n o m e levels i n serial s a m p l e s of p a t i e n t s b e f o r e i n t e r f e r o n treatment. F Giostra, R Francesconi, P Groff, A Manzin, L Solforosi, F Lari, G Ballardini, M Lenzi, M Clementi and FB Bianchi. Med. Interna II, Univ. di Bologna and Inst. di Virologia, Univ. di Ancona, Italy.
Q u a s i s p e c i e s of t h e h e p a t i t i s C v i r u s in acute a n d c h r o n i c i n f e c t i o n . T Peters, O Czerwinski, HJ Schlayer, B Hiller, HE Blum and J Rasenack. Univ. of Freiburg, Dept. of Internal Med., Freiburg, Germany.
#944
T h e s e v e r i t y o f l i v e r d i s e a s e influences HCV replication in p a t i e n t s w i t h c h r o n i c h e p a t i t i s C. C Duvoux, JM Pawlotsky, A Bastie, D Cherqui, JM M~treau, J Duval and D Dhumeaux: Liver Transplant Unit and Dept. of Virol., Hop. Henri Mondor, Univ. Paris XII, Creteil, France.
H L P A T I T I S C: G E N O T Y P I N G / EPIDEMIOLOGY / COMPLICATIONS #945
Hepatitis C v i r u s g e n o t y p e s a n d clinical c o u r s e of anti-HCV p o s i t i v e cirrhosis. L Benvegnfl, P Pontisso, D Cavalletto, L Chemello and A Alberti. Clinica Med. II, Univ. of Padua, Italy.
#946
L a c k of a s s o c i a t i o n b e t w e e n type of h e p a t i t i s C virus, s e r u m load a n d s e v e r i t y of liver d i s e a s e . R Romeo, M Colombo, MG Rumi, R Soffredini, E Del Ninno, MF Donato, A Russo and P Simmonds. Inst. of Internal Med., Univ. of Milan and Epidemiol. Unit, Aviano Cancer Ctr., Aviano, Italy; Dept. of Med. Microbiol., Univ. of Edinburgh, Scotland.
Q u a s i s p e c i e s of h e p a t i t i s C v i r u s a n d its clinical r e l e v a n c e to h i g h s e r u m level of i m m u n e - c o m p l e x . M Zeniya, A Kuramoto, Y Okuaki, Y Ohkawa, H Fukata, M Hara, H Takahashi, F Watanabe, Y Aizawa and G Toda. Dept. of Internal Med., Jikei Univ. Sch. of Med., Tokyo, Japan.
T h e s p o n t a n e o u s f l u c t u a t i o n of v i r e m i a o v e r t i m e in u n t r e a t e d patients with chronic type C hepatitis. SC Gordon, BA Khan, CF Dmuchowski, VP Kodali and AL Silverman. Div. of Gastro.-Hepatol., William Beaumont Hosp., Royal Oak, MI.
#938
#942
T h e "take-over" p h e n o m e n o n in patients infected with multiple hepatitis C virus genotypes. KP Qian, SN Natov, BJG Pereira and JYN Lau. Section of Hepatobiliary Diseases, Univ. of Florida, Gainesville, FL; New England Med. Ctr., Boston, MA.
Rapid quasispecies analysis of the hepatitis C virus b y fluorescent single strand conformation polymorphism. T Peters, H Schlayer, B Hiller, B R~sler, HE Blum and J Rasenack. Dept. of Internal Med., Albert-Ludwigs-Univ., Freiburg, Germany.
D y n a m i c c h a n g e s of f l o a t i n g d e n s i t y in c i r c u l a t i n g h e p a t i t i s C viral particles a s s o c i a t e d w i t h d i s e a s e activity. K Hino, K Fujii, M Okazaki, M Korenaga, C Murakami, M Okuda and K Okita. Dept. of Internal Med., Yamagnchi Univ. Sch. of Med., Yamaguchi, Japan.
#936
#941
H e p a t i t i s C v i r u s c a r r i e r s w i t h normal ALT activity: v i r e m i a , g e n o t y p e , histology and effect of interferon therapy. J Areias, I Pedroto, T Freitas, R Cerqueira, R Teixeira, L Pinho, B Justifa and AM Saraiva. Depts. of Gastro. and Hematol., Hosp. Geral de Santo Antonio, Oporto, Portugal.
#947
G e n o m i c c h a r a c t e r i z a t i o n of HCV i n v i r e m i c p a t i e n t s w i t h n o r m a l ALT. P Pontisso, MG Ruvoletto, M Gerotto, L De Moliner, S Tisminetzky, L Chemello, C Casarin, F Baralle and A Alberti. Clinica Med. 2, Univ. of Padua, ICGEB, Trieste, Italy.
AASLD PROGRAM
II......
Tuesday Posters
H E P A T I T I S C: P A T H O G E N E S I S
#893
Specific inhibition of hepatitis C virus core antigen production by antisense RNA constructs. T Wakita, D Moradpour, K Tokushige and JR Wands. Molecular Hepatol. Lab., Massachusetts Gen. Hosp. Cancer Ctr. and Harvard Med. Sch,, Charlestown, MA.
#894
Specific inhibition of hepatitis C virus gene expression by antisense oligonueleotides in a tightly regulated cell culture system.
#900 Efficient production of a portion of the hepatitis C core protein and analysis of the murine i m m u n e response to recombinant protein: toward a vaccine for hepatitis C. Y Hitomi, WM McDonnell, JR Baker, Jr and FK Askari. Dept. of Internal Med., Div. of Gastro. and Allergy & Immunol., Univ. of Michigan Med. Ctr. and Veterans Admin. Med. Ctr., Ann Arbor, MI.
#901
D Moradpour, T Wakita, C Englert and JR Wands. Molecular Hepatol. Lab. and Lab. of Molecular Genetics, Massachusetts Gen. Hosp. Cancer Ctr., Harvard Med. Sch., Charlestown, MA.
DNA. CH Wu, A Siddiqui and GY Wu. Dept. of Med., Div. of Gastro.,, Univ. of Connecticut Sch. of Med., Farmington, CT; Dept. of Microbiol., Univ. of Colorado HSC, Denver, CO.
#902 Maturation and biological importance of hepatitis C virus core protein. K Yasui, T Wakita, KT Kohara, M Ichikawa, T Kajita, A Hasegawa and M Kohara. The Tokyo Metro. Inst. of Med. Science, Tokyo, 3rd Dept. of Internal Med., Kyoto Prefectural Univ. of Med., Kyoto, The Tokyo Metro. Inst. for Neuroscience, Internatl. Reagents Co. and Corp. Research & Development Lab., Tonen Co., Tokyo, Japan.
Inhibition of hepatitis C virusdirected translation by hammerhead ribozymes in vitro. N Sakamoto, CH Wu and GY Wu. Dept. of Med., Div. of Gastro.-Hepatol., Sch. of Med., Univ. of Connecticut Health Ctr., Farmington, CT.
#897 Expression of enzymatically active HCV NS3 protease (genotype lb) and the development of an in vitro protease assay system. JA Feller, AR Carroll, DV Sangar, JYN Lau and BE Clarke. Section of Hepatobiliary Diseases, Dept. of Med., Univ. of Florida, Gainesville, FL; Wellcome Research Labs, Beckenham, England.
HCV core protein subcellular localization: analysis by confocal and electron microscopy and correlation with the cell cycle. G Barba, T Harada, F Harper, Y Matsuura, T Miyamura and C Br~chot. Liver Cancer and Molecular Virol., INSERM U370, Paris, France; Dept. ofVirol. II, Natl. Inst. of Health, Tokyo, Japan; Lab. de Biol. et Ultrastructure du Noyau, CNRS UPR272, Villejuif, France.
#895 Targeted inhibition of HCV promoter activity using HCV-specific antisense
#896
HEPATOLOGYOctober 1995
#903
Single-strand conformation p o l y m o r p h i s m analysis of the HCV h y p e r v a r i a b l e region. RR Lareu, WD Reed and NR Swanson. Univ. Dept. Med. and Dept. Gastro., QEIIMC, Nedlands, Australia.
#904 Increased variability in the HCV RNA hypervariable region among patients with HCV/HIV co-infection.
#898 Antibody response to hypervariable region of HCV differs between genotype lb and 2a.
KE Sherman, C Andreatta, J O'Brien, A Gutierrez and R Harris. Dept. of Med. and Pathol., Univ. of Cincinnati Med. Ctr., Cincinnati, OH; Fitzsimons Army Med. Ctr., Aurora, CO.
K Yoshioka, T Aiyama, A Okumura, M Takayanagi, K Iwata, T Ishikawa and S Kakumu. 3rd Dept. of Internal Med., Nagoya Univ. Sch. of Med., Nagoya, Japan.
#905 In vitro effects of interferon alpha-2B on hepatitis C virus replication in lymphocytes.
#899 Expression and characterization of the NS5B (RNA-dependent RNA polymerase) gene of hepatitis C virus. RH Al, Y Wang, Y xie and CH Hagedorn. Div. of Digestive Diseases, Emory Univ. Sch. of Med. and VA Med. Ctr., Atlanta, GA.
X Wang and AH Sherker. Lady Davis Inst. for Med. Research, SMBD-Jewish Gen. Hosp., McGill Univ., Montreal, Canada.
#906 Molecular mapping of an intrahepatic hepatitis C virus-specific crossgenotype cytotoxic T lymphocyte epitope. CG Marousis, DR Nelson and JYN Lau. Section of Hepatobiliary Diseases, Univ. of Florida, Gainesville, FL.
HEPATOLOGYVol. 22, No. 4, Pt. 2, 1995
AASLD PROGRAM
91A
Tuesday Posters #907
The p e r i p h e r a l b l o o d c y t o t o x i c T l y m p h o c y t e r e s p o n s e to HCV does n o t r e f l e c t d i s e a s e activity during c h r o n i c HCV infection. B Rehermann, KM Chang, JG McHutchison, RP Kokka, M Houghton and FV Chisari. Scripps Research Inst., La Jolla and Chiron Corp., Emeryville, CA.
#913
CD30-induction on T l y m p h o c y t e s by HCV-derived a n t i g e n s in p a t i e n t s w i t h c h r o n i c h e p a t i t i s C. M Lechmann, B Irrgang, HG Ihlenfeldt, G Jung, T Sauerbruch and U Spengler. Dept. of Gen. Med., Univ. of Bonn and Inst. of Organic Chem., Univ. of Tuebingen, Germany.
#908
Viral e s c a p e from the HI,A-A2 r e stricted cytotoxic T lymphocyte r e s p o n s e is n o t c o m m o n during c h r o n i c h e p a t i t i s C virus infection. KM Chang, B Rehermann, JG McHutchison, C Pasquinelli and FV Chisari. Scripps Research Inst., La Jolla, CA.
#914
C y t o k i n e expression and T-cell subset activation in chronic hepatitis C. RP Woitas, M Sippel, A Blaufuss, T Sauerbruch and U Spengler. Dept. of Gen. Med., Univ. of Bonn, Germany.
#915
T h l c y t o k i n e p a t t e r n and d e f e c t i v e IL-10 in c h r o n i c h e p a t i t i s C virusinfected livers. AS Gaweco, DH Van Thiel, V Rustgi, T Hassanein, L E1-Ashmawy, T Whiteside, WJ Hofmann and HF Otto. Inst. of Pathol., Univ. of Heidelberg, Germany; Oklahoma Transplant Inst., Oklahoma City, OK; INOVA Inst., Fairfax, VA; Dept. of Pathot., Univ. of Pittsburgh, PA.
#916
Immunodominant hepatitis C virus core epitopes s t i m u l a t e CD3÷/CD30 + l y m p h o c y t e s for p r o d u c t i o n o f TH0like c y t o k i n e s i n p a t i e n t s w i t h chronic h e p a t i t i s C. RP Woitas, M Lechmann, HG Ihlenfeldt, G Jung, T Sauerbruch and U Spengler. Depts. of Gen. Med., Univ. of Bonn and Inst. of Organic Chem., Univ. of Tuebingen, Germany.
#917
D e f e c t i v e a n t i v i r a l c y t o k i n e s in chronic h e p a t i t i s C v i r u s - i n f e c t e d livers. AS Gaweco, V Rustgi, T Hassanein, L E1-Ashmawy, T Whiteside, WJ Hofmann, DH Van Thiel and HF Otto. Inst. of Pathol., Univ. of Heidelberg, Germany; INOVA Inst., Fairfax, VA; Oklahoma Transplant Inst., Oklahoma City, OK; Dept. of Pathol., Univ. of Pittsburgh, PA.
#918
Quantitative analysis o f i n t e r f e r o n a/~ r e c e p t o r m R N A in the liver o f patients w i t h c h r o n i c h e p a t i t i s C. H Yatsuhashi, MC Parquet, K Yamasaki, F Iio, T Aritomi, M Yamasaki, O Inoue, M Koga and M Yano. Inst. for Clinical Research, Nagasaki Chuo Natl. Hosp., Nagasaki, Japan.
#919
Changes in s e r u m soluble interleukin-2 r e c e p t o r l e v e l s in p a t i e n t s w i t h chronic h e p a t i t i s C b y interferon-a. T Takeda, S Nishiguchi, S Nakajima, S Shiomi, T Kuroki and K Kobayashi. 3rd Dept. of Internal Med., Osaka City Univ. Med. Sch., Osaka, Japan.
#909
C o m p a r a t i v e analysis o f the cytotoxic T cell r e s p o n s e in c h r o n i c HBV and HCV infection. B Rehermann, KM Chang, JG McHutchison and FV Chisari. Scripps Research Inst., La Jolla, CA,
#910
H e p a t i t i s C virus-specific cytotoxic T l y m p h o c y t e s f r o m liver a n d peripheral blood of a patient with a s u s t a i n e d r e s p o n s e to i n t e r f e r o n therapy. DKH Wong, MJ Koziel, D Dudley, JT Wang, N Afdahl, C Rice, M Houghton and BD Walker. Infectious Disease Unit, Massachusetts Gen. Hosp. and Boston City Hosp., Boston, MA; Washington Univ., St. Louis, MO; Chiron Corp., Emeryville, CA.
#911
#912
A c c u m u l a t i o n o f HCV-core specific T cell c l o n o t y p e s in patients w i t h c h r o n i c h e p a t i t i s C and its r e d u c t i o n f o l l o w i n g e r a d i c a t i o n o f HCV-RNA by antiviral treatment. A Tanaka, H Kita, M Takatori, S Iwabuchi, K Kurokawa, M Imawari, K Nishioka, S Iino and K Yamamoto. Inst. ofMed. Sci. and 2nd Dept. of Internal Med., St. Marianna Univ., Sch. ofMed., Kanagawa, Liver Study Lab., Jichi Univ., Sch. ofMed., Tochigi and 1st and 3rd Dept. of Internal Med., Univ. of Tokyo, Japan. Viral c l e a r a n c e d u r i n g a c u t e h e p a t i t i s C is associated w i t h a NS3-specific CD4 + T cell response: functional and m o l e c u l a r analysis at the clonal level. HM Diepolder, R Zachoval, RM Hoffmann, EA Wierenga, T Santantonio, M-C Jung, M W~ichtler, D Eichenlaub and GR Pape. Inst. for Immunol. and Dept. of Med. II, Klinikum Grosshadern, Univ. of Munich, Germany.
92A
AASLD PROGRAM
HEPATOLOGYOctober 1995
Tuesday Posters #920
T r a n s f o r m i n g g r o w t h factor-~l in c h r o n i c h e p a t i t i s C.
#927
DR Nelson, CG Marousis, KP Qian, Y Xu, RP Gonzalez-Peralta, GL Davis and JYN Lau. Section of Hepatobiliary Diseases, Univ. of Florida, Gainesville, FL.
#921
P a t t e r n s of i m m u n e r e s p o n s e s to the h o s t - e n c o d e d GOR a n d h e p a t i t i s C virus core-derived epitopes with relation to hepatitis C viremia and d i s e a s e severity.
K Fujii, K Hino, M Okazaki, M Korenaga, C Murakami, M Okuda, M Ikeda, S Kondoh and K Okita. Dept. of Internal Med., Yamaguchi Univ. Sch. of Med., Yamaguchi, Japan.
#928
JA Quiroga, M Pardo, S Navas, J Martin and V Carrefio. Hepatol. Unit, Fundacion Jimenez Diaz, Madrid, Spain.
#922
#923
#924
E x p r e s s i o n of CD80 (B7/BB-1) in l i v e r t i s s u e s of p a t i e n t s w i t h c h r o n i c h e p a t i t i s C. K Mochizuki, N Hayashi, K Katayama, N Hiramatsu, Y Kawanishi, A Kasahara, H Fusamoto, T Yokochi and T Kamada. 1st Dept. of Med., Osaka Univ. Sch. of Med., Suita and Dept. of Bacteriol., Aichi Med. Univ. Sch., Aichi, Japan.
P h e n o t y p i c a n d f u n c t i o n a l charact e r i z a t i o n of i n t r a h e p a t i c n a t u r a l killer a n d m a c r o p h a g e s i n viral chronic hepatitis.
#925
#926
#929
R e l e v a n c e of u l t r a s t r u c t u r a l l y m p h o c y t e e m p e r i p o l e s i s in h e p a t o c y t e s in c h r o n i c h e p a t i t i s C.
#931
K Fukuda, S Nishiguchi, T Kuroki and K Kobayashi. 3rd Dept. of Internal Med., Osaka City Univ. Med. Sch., Osaka, Japan.
D-related h u m a n l e u k o c y t e a n t i g e n s a n d d i s e a s e s e v e r i t y in c h r o n i c h e p a t i t i s C. AJ Czaja, HA Carpenter, PJ Santrach and SB Moore. Mayo Clinic, Rochester, MN.
Virological c h a r a c t e r i z a t i o n of hepatitis C v i r u s h e a l t h y carriers. M Debette, J Coste, V Hyrailles, P Blanc, A Dubois, H Michel and D Larrey. Serv. d'Hepatogastro., Hop. Saint-Eloi and Ctr. de Transfusion Sanguine, Montpellier, France.
#932
HCV infection of peripheral blood m o n o n u c l e a r cells assessed b y in s i t u reverse t r a n s c r i p t a s e - p o l y m e r a s e c h a i n reaction: a pathogenetic role in HCV-related m i x e d cryoglobulinemia?
H e p a t i t i s C v i r u s r e p l i c a t i o n in p e r i p h e r a l b l o o d m o n o n u c l e a r cells i n r e l a t i o n to r e s p o n s e to i n t e r f e r o n therapy.
L o w e x p r e s s i o n of e r y t h r o c y t e c o m p l e m e n t r e c e p t o r type I i n d u c e s defective i m m u n e c o m p l e x c l e a r a n c e in c h r o n i c h e p a t i t i s C p a t i e n t s . T Kanto, N Hayashi, T Takehara, K Katayama, A Kasahara, H Fusamoto, M Kato, M Akiyama and T Kamada. 1st Dept. of Med., Osaka Univ. Med. Sch., Suita, Dept. of Gastro., Osaka Natl. Hosp., Osaka and Dept. of Med., Shin-Senri H0sp., Suita, Japan.
#930
L Muratori, M Lenzi, D Gibellini, M Frisoni, M Cataleta, P Muratori and FB Bianchi. Depts. of Internal Med., Inst. of Microbiol. and Rheumatol., Univ. of Bologna, S. Orsola-Malpighi, Bologna, Italy.
Immunological events during the i n c u b a t i o n p e r i o d of h e p a t i t i s C v i r u s i n f e c t i o n in i m m u n o c o m p r o m i s e d individuals. M Psichogiou, A Katsoulidou, E Vaindirli, B Francis, SR Lee and A Hatzakis. Athens Univ. Med. Sch., Athens, Greece; Ortho Diagnostic Systems Inc., Raritan, NJ.
C Garc~a-MonzSn, L Garc~a-Buey, MJ Borque, A Garcia-S~nchez, JM Pajares and R Moreno-Otero. Liver Unit, Molecular Biol. Unit and Pathol. Serv., Hosp. de la Princesa, Univ. Autonoma de Madrid, Spain.
J Gabanyi, D Vetter, M Doffoel, A Ananna, G Uhl and JM Vetter. Serv. d'Hepatogastro. and Inst. d'Anatomie Pathol., Hop. Civil, Strasbourg, France.
Different q u a s i s p e c i e s of h e p a t i t i s C v i r u s RNA b e t w e e n s e r u m a n d perip h e r a l b l o o d m o n o n u c l e a r cells.
Chronic hepatitis C virus infection in HIV p o s i t i v e patients: i n f l u e n c e of h o s t i m m u n i t y . L Serfaty, C Housset, L Morand-Joubert, J Frottier, J Lebas, PP De Saint-Maur and R Poupon. Unite d'Hepatol., Serv. de Bacteriol.-Virol., Maladies Infectieuses, Med. Interne and Anatomopathol., Hop. St.-Antoine, Paris, France.
#933
C h r o n i c h e p a t i t i s C of d r u g users: i n f l u e n c e of HIV i n f e c t i o n . S Pol, N Trinh Thi, V Thiers, F Jaffredo, F Carnot, B Lamorthe, H Zylberberg, P Berthelo~, C Br~chot and B Nalpas. Liver Unit, INSERM U-370, Hop. Necker and Hybridotest, Inst. Pasteur, Paris, France.
HEPATOLOGYVol. 22, No. 4, Pt. 2, 1995
AASLD PROGRAM
93A
Tuesday Posters #934
Hepatitis C v i r u s density heterogeneity a n d v i r a l t i t r e in acute a n d c h r o n i c infection: a comparison of immunodeficient a n d i m m u n o c o m p e t e n t patients. JP Watson, DJ Bevitt, GP Spickett, GL Toms and MF Bassendine. Depts. ofMed., Virol. and Immunol., Univ. of Newcastle-uponTyne, England.
#935
#937
S p o n t a n e o u s f l u c t u a t i o n s of viral l o a d in u n t r e a t e d p a t i e n t s w i t h c h r o n i c h e p a t i t i s C. M Bourli~re, P Halfon, P Berthezene, H Khiri, I Portal, C Wartelle, V Gerolami, G Cartouzou and AP Gauthier. Liver Unit, Hop. Saint Joseph, Hop. de la Conception, Alpha Bio. and INSERM U315, Marseille, France.
#939
#940
#943
Wide f l u c t u a t i o n of p l a s m a t i c HCV g e n o m e levels i n serial s a m p l e s of p a t i e n t s b e f o r e i n t e r f e r o n treatment. F Giostra, R Francesconi, P Groff, A Manzin, L Solforosi, F Lari, G Ballardini, M Lenzi, M Clementi and FB Bianchi. Med. Interna II, Univ. di Bologna and Inst. di Virologia, Univ. di Ancona, Italy.
Q u a s i s p e c i e s of t h e h e p a t i t i s C v i r u s in acute a n d c h r o n i c i n f e c t i o n . T Peters, O Czerwinski, HJ Schlayer, B Hiller, HE Blum and J Rasenack. Univ. of Freiburg, Dept. of Internal Med., Freiburg, Germany.
#944
T h e s e v e r i t y o f l i v e r d i s e a s e influences HCV replication in p a t i e n t s w i t h c h r o n i c h e p a t i t i s C. C Duvoux, JM Pawlotsky, A Bastie, D Cherqui, JM M~treau, J Duval and D Dhumeaux: Liver Transplant Unit and Dept. of Virol., Hop. Henri Mondor, Univ. Paris XII, Creteil, France.
H L P A T I T I S C: G E N O T Y P I N G / EPIDEMIOLOGY / COMPLICATIONS #945
Hepatitis C v i r u s g e n o t y p e s a n d clinical c o u r s e of anti-HCV p o s i t i v e cirrhosis. L Benvegnfl, P Pontisso, D Cavalletto, L Chemello and A Alberti. Clinica Med. II, Univ. of Padua, Italy.
#946
L a c k of a s s o c i a t i o n b e t w e e n type of h e p a t i t i s C virus, s e r u m load a n d s e v e r i t y of liver d i s e a s e . R Romeo, M Colombo, MG Rumi, R Soffredini, E Del Ninno, MF Donato, A Russo and P Simmonds. Inst. of Internal Med., Univ. of Milan and Epidemiol. Unit, Aviano Cancer Ctr., Aviano, Italy; Dept. of Med. Microbiol., Univ. of Edinburgh, Scotland.
Q u a s i s p e c i e s of h e p a t i t i s C v i r u s a n d its clinical r e l e v a n c e to h i g h s e r u m level of i m m u n e - c o m p l e x . M Zeniya, A Kuramoto, Y Okuaki, Y Ohkawa, H Fukata, M Hara, H Takahashi, F Watanabe, Y Aizawa and G Toda. Dept. of Internal Med., Jikei Univ. Sch. of Med., Tokyo, Japan.
T h e s p o n t a n e o u s f l u c t u a t i o n of v i r e m i a o v e r t i m e in u n t r e a t e d patients with chronic type C hepatitis. SC Gordon, BA Khan, CF Dmuchowski, VP Kodali and AL Silverman. Div. of Gastro.-Hepatol., William Beaumont Hosp., Royal Oak, MI.
#938
#942
T h e "take-over" p h e n o m e n o n in patients infected with multiple hepatitis C virus genotypes. KP Qian, SN Natov, BJG Pereira and JYN Lau. Section of Hepatobiliary Diseases, Univ. of Florida, Gainesville, FL; New England Med. Ctr., Boston, MA.
Rapid quasispecies analysis of the hepatitis C virus b y fluorescent single strand conformation polymorphism. T Peters, H Schlayer, B Hiller, B R~sler, HE Blum and J Rasenack. Dept. of Internal Med., Albert-Ludwigs-Univ., Freiburg, Germany.
D y n a m i c c h a n g e s of f l o a t i n g d e n s i t y in c i r c u l a t i n g h e p a t i t i s C viral particles a s s o c i a t e d w i t h d i s e a s e activity. K Hino, K Fujii, M Okazaki, M Korenaga, C Murakami, M Okuda and K Okita. Dept. of Internal Med., Yamagnchi Univ. Sch. of Med., Yamaguchi, Japan.
#936
#941
H e p a t i t i s C v i r u s c a r r i e r s w i t h normal ALT activity: v i r e m i a , g e n o t y p e , histology and effect of interferon therapy. J Areias, I Pedroto, T Freitas, R Cerqueira, R Teixeira, L Pinho, B Justifa and AM Saraiva. Depts. of Gastro. and Hematol., Hosp. Geral de Santo Antonio, Oporto, Portugal.
#947
G e n o m i c c h a r a c t e r i z a t i o n of HCV i n v i r e m i c p a t i e n t s w i t h n o r m a l ALT. P Pontisso, MG Ruvoletto, M Gerotto, L De Moliner, S Tisminetzky, L Chemello, C Casarin, F Baralle and A Alberti. Clinica Med. 2, Univ. of Padua, ICGEB, Trieste, Italy.