- Email: [email protected]
Hepatoprotective activity of Berberis integerrima Bge extract in rats treated with CCl4: In vitro and in vivo studies
S310
Abstracts / Toxicology Letters 164S (2006) S1–S324
of this study was to investigate any positive effects or possible side effects of the use of pollen. In this study, mature male rats were fed to three kinds of pollen (Trifolium spp., Raphanus spp., and Cistus spp.) in the amount of 60 mg/per animal/per day for a 30-day period. At the end of the treatment, biochemical parameters and serum enzyme activities were analysed and weights of liver and kidney were recorded. Also, the liver and the kidney tissues of rats were examined at light microscopic level. There were decreases in serum cholesterol and HDL levels of rats fed pollen of Trifolium spp. and Cistus spp. There was no change in serum enzyme level of rats at pollen groups. No change in relative weights of liver and kidney of rats in pollen groups was determined. Histopathological change in the liver and kidney of rats given pollen was not observed. The liver and kidney tissues of pollen treated rats were similar to those of control rat. According to the results of this study, although serum cholesterol and HDL levels decreased, we cannot suggest that bee pollen did not caused adverse and benefit effects, because of duration of pollen treatment was only 30 days. doi:10.1016/j.toxlet.2006.07.302 P26-16 Hepatoprotective activity of Berberis integerrima Bge extract in rats treated with CCl4 : In vitro and in vivo studies Akram Jamshidzadeh, Hossien Niknahad Faculty of Pharmacy, Shiraz University of Medical Sciences, Iran Berberis integerrima is an edible plant employed in the Asian Traditional Medicine, particularly its fruits being used as a tonic remedy for liver and heart. In this investigation, freshly isolated rat hepatocytes and rats were used as the in vitro and in vivo models to evaluate the hepatoprotective activity of aqueous extract of dried powdered fruits of Berberis integerrima. CCl4 was selected as hepatotoxin. Silymarin was the reference hepatoprotective agent. In the in vitro study, Trypan Blue Exclusion test was the criteria for cell viability. Freshly isolated rat hepatocytes (1 × 106 cells/ml) were treated with CCl4 (10 mM/ml) and various concentrations of extract (50, 150, 300, 400, 500, 800 g/ml) for 3 h. The Berberis integerrima extract with concentrations of 300, 400 and 500 g/ml protected the cells against CCl4 -induced cytotoxicity, but concentration of 800 g/ml increased the CCl4 -induced cytotoxicity. In the in vivo study, serum aminotransaminase (AST), alanine aminotransferase (ALT) and alkaline phos-
phatase (ALP) activities and histopathological examination were the criteria for evidences of liver injury. CCl4 /olive oil (1:1, 3 ml/kg, i.p.) caused the increase in AST, ALT, ALP and centrilobular hydropic degeneration of hepatocytes. Pretreatment and treatment of animals with a single oral dose of extract (30 mg/kg), for 3 days before and 12 h after CCl4 injection showed that the Berberis integerrima extract could protect the liver against CCl4 -induced damages. doi:10.1016/j.toxlet.2006.07.309 P26-17 Effects of subchronic exposure to camphor on male reproductive tract in rats Akram Jamshidzadeh, Javad Sajedianfard Faculty of Pharmacy, Shiraz University of Medical Sciences, Iran Camphor is extensively use in pharmaceutical preparations and skin care products. The effect of camphor on mail reproductive system is controversial. In this study, the effects of camphor on reproductive tract were studied in Sprague–Dawley male rats. Several groups of adult rats were treated (i.p.) with 5, 10 and 20 mg/kg of camphor solution for 30 days. After this period, rats were scarified and their testis, seminal vesicle, epidydimis as well as afferent duct were removed for histological examination using hematoxylin–eosin staining. The results indicated a decrease in the body weight and testis size and weight with all of the experimental doses. Testicular sperm number and motility were also decreased in all of the treated rats. Higher doses (10 and 20 mg/kg) of camphor caused morphological changes and a toxic effect on sperm and their motility, as well. Therefore, it is concluded that camphor with even the lowest dose used in this study can cause damage to male reproductive system. doi:10.1016/j.toxlet.2006.07.310
Abstracts / Toxicology Letters 164S (2006) S1–S324
of this study was to investigate any positive effects or possible side effects of the use of pollen. In this study, mature male rats were fed to three kinds of pollen (Trifolium spp., Raphanus spp., and Cistus spp.) in the amount of 60 mg/per animal/per day for a 30-day period. At the end of the treatment, biochemical parameters and serum enzyme activities were analysed and weights of liver and kidney were recorded. Also, the liver and the kidney tissues of rats were examined at light microscopic level. There were decreases in serum cholesterol and HDL levels of rats fed pollen of Trifolium spp. and Cistus spp. There was no change in serum enzyme level of rats at pollen groups. No change in relative weights of liver and kidney of rats in pollen groups was determined. Histopathological change in the liver and kidney of rats given pollen was not observed. The liver and kidney tissues of pollen treated rats were similar to those of control rat. According to the results of this study, although serum cholesterol and HDL levels decreased, we cannot suggest that bee pollen did not caused adverse and benefit effects, because of duration of pollen treatment was only 30 days. doi:10.1016/j.toxlet.2006.07.302 P26-16 Hepatoprotective activity of Berberis integerrima Bge extract in rats treated with CCl4 : In vitro and in vivo studies Akram Jamshidzadeh, Hossien Niknahad Faculty of Pharmacy, Shiraz University of Medical Sciences, Iran Berberis integerrima is an edible plant employed in the Asian Traditional Medicine, particularly its fruits being used as a tonic remedy for liver and heart. In this investigation, freshly isolated rat hepatocytes and rats were used as the in vitro and in vivo models to evaluate the hepatoprotective activity of aqueous extract of dried powdered fruits of Berberis integerrima. CCl4 was selected as hepatotoxin. Silymarin was the reference hepatoprotective agent. In the in vitro study, Trypan Blue Exclusion test was the criteria for cell viability. Freshly isolated rat hepatocytes (1 × 106 cells/ml) were treated with CCl4 (10 mM/ml) and various concentrations of extract (50, 150, 300, 400, 500, 800 g/ml) for 3 h. The Berberis integerrima extract with concentrations of 300, 400 and 500 g/ml protected the cells against CCl4 -induced cytotoxicity, but concentration of 800 g/ml increased the CCl4 -induced cytotoxicity. In the in vivo study, serum aminotransaminase (AST), alanine aminotransferase (ALT) and alkaline phos-
phatase (ALP) activities and histopathological examination were the criteria for evidences of liver injury. CCl4 /olive oil (1:1, 3 ml/kg, i.p.) caused the increase in AST, ALT, ALP and centrilobular hydropic degeneration of hepatocytes. Pretreatment and treatment of animals with a single oral dose of extract (30 mg/kg), for 3 days before and 12 h after CCl4 injection showed that the Berberis integerrima extract could protect the liver against CCl4 -induced damages. doi:10.1016/j.toxlet.2006.07.309 P26-17 Effects of subchronic exposure to camphor on male reproductive tract in rats Akram Jamshidzadeh, Javad Sajedianfard Faculty of Pharmacy, Shiraz University of Medical Sciences, Iran Camphor is extensively use in pharmaceutical preparations and skin care products. The effect of camphor on mail reproductive system is controversial. In this study, the effects of camphor on reproductive tract were studied in Sprague–Dawley male rats. Several groups of adult rats were treated (i.p.) with 5, 10 and 20 mg/kg of camphor solution for 30 days. After this period, rats were scarified and their testis, seminal vesicle, epidydimis as well as afferent duct were removed for histological examination using hematoxylin–eosin staining. The results indicated a decrease in the body weight and testis size and weight with all of the experimental doses. Testicular sperm number and motility were also decreased in all of the treated rats. Higher doses (10 and 20 mg/kg) of camphor caused morphological changes and a toxic effect on sperm and their motility, as well. Therefore, it is concluded that camphor with even the lowest dose used in this study can cause damage to male reproductive system. doi:10.1016/j.toxlet.2006.07.310