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Heterologous expression of Arabidopsis Npr1 modulates insect resistance and abiotic stress tolerance also in tobacco transgenic plants
Journal of Biotechnology 136S (2008) S236–S246
Contents lists available at ScienceDirect
Journal of Biotechnology journal homepage: www.elsevier.com/locate/jbiotec
Abstracts
IV-3 Transgenic crops and animals
IV3-O-002 Heterologous expression of Arabidopsis Npr1 modulates insect resistance and abiotic stress tolerance also in tobacco transgenic plants T. Srinivasan, K.R. Rajesh Kumar, G. Meur, P.B. Kirti ∗ University of Hyderabad, Department of Plant Sciences, Hyderabad 500046, India E-mail address: [email protected] (P.B. Kirti). In Arabidopsis, NPR1 has been shown to modulate the salicylic acid induced systemic acquired resistance (SAR), and its biology has been reviewed extensively (Dong, 2004). It interacts with TGA factors upon nuclear localization, and enhances the expression of pathogenesis related proteins. Constitutive expression of AtNPR1 in Arabidopsis and heterologous systems like tomato and rice has been shown to enhance the plant’s resistance to diseases (Cao et al., 1998; Friedrich et al., 2001; Lin et al., 2004). We have demonstrated that the overexpression of AtNPR1 in transgenic tobacco has enhanced the host plant’s resistance against generalist herbivore, Spodoptera litura, at least to the early instars (Meur et al., 2008). High expression lines of transgenic tobacco also exhibited enhanced tolerance to abiotic and oxidative stress treatments and the tolerance was associated with constitutive upregulation of transcripts for pathogenesis related proteins like PR1, PR2 (glucanase) and PR5 (thaumatin like protein) along with proteins involved in abiotic stress like ascorbate peroxidase, Cu/Zn superoxide dismutase. Constitutive expression of PR1 has been shown to enhance abiotic stress tolerance earlier (Sarowar et al., 2005). Our studies demonstrate that constitutive expression of NPR1 enhances the plant’s overall ability to counter the various stresses, biotic as well as abiotic. Hence, it is a very valuable gene for deployment in transgenic crops.
References Cao, H., Li, X., Dong, X., 1998. Generation of broad-spectrum disease resistance by overexpression of an essential regulatory gene in systemic acquired resistance. Proc. Natl. Acad. Sci. U.S.A. 95, 6531–6536. Dong, X., 2004. NPR1, All things considered. Curr. Opin. Plant Biol. 7, 547–552. Friedrich, L., Lawton, K., Dietrich, R., Willits, M., Cade, R., Ryals, J., 2001. NIM1 overexpression in Arabidopsis potentiates plant disease resistance and results in enhanced effectiveness of fungicides. Mol. Plant Microbe Interact. 14, 1114–1124. Lin, W.C., Lu, C.F., Wu, J.W., Cheng, M.L., Lin, Y.M., Yang, N.S., Black, L., Green, S.K., Wang, J.F., Cheng, C.P., 2004. Transgenic tomato plants expressing the Arabidopsis
0168-1656/$ – see front matter
NPR1 gene display enhanced resistance to a spectrum of fungal and bacterial diseases. Transgenic Res. 13, 567–581. Meur, G., Budatha, M., Srinivasan, T., Rajesh Kumar, K.R., Datta.Gupta, A., Kirti, P.B., 2008. Overexpression of Arabidopsis NPR1 in tobacco confers enhanced resistance to the early instars of Spodoptera litura. Physiol. Plant. [Epub ahead of print] PMID: 18397206. Sarowar, S., Kim, Y.J., Kim, E.N., Kim, K.D., Hwang, B.K., Islam, R., Shin, J.S., 2005. Overexpression of a pepper basic pathogenesis-related protein-1 gene in tobacco plants enhances resistance to heavy metals and pathogen stress. Plant Cell Rep. 24, 216–224.
doi:10.1016/j.jbiotec.2008.07.501 IV3-O-003 Expression of a mustard annexin confers abiotic stress tolerance and improved fiber quality in transgenic cotton K. Divya, P.B. Kirti ∗ University of Hyderabad, Department of Plant Sciences, Hyderabad 500046, India E-mail address: [email protected] (P.B. Kirti). Cotton is a high priority commercial crop and its fiber quality is dependent upon the expression of genes like sucrose phosphate synthase, sucrose synthase and cellulose synthase. Upregulation of sucrose phosphate synthase enhanced fiber quality in normal conditions (Haigler et al., 2007). Annexins belong to a multigene family of Ca2+ dependent, phospholipid and cytoskeleton binding proteins and play a role in regulating plant responses to various abiotic stress conditions (Cantero et al., 2006). Insertion mutants of annAt1 of Arabidopsis are hypersensitive to osmotic stress and ABA during seed germination and the seedling growth indicating that annAt1 acts in a narrow developmental window at the early stages of the plant growth (Lee et al., 2004). Annexins may also serve in anchoring the accessory proteins such as sucrose synthase converting sucrose into fructose and UDP-glucose in the cotton fiber plasma membrane (Delmer and Pothika, 1997) and the implication of AnnGh1 has been suggested in cellulose synthase complex. AnnAt1 has been shown to have a potential role in scavenging free radicals developed under stress, because of the peroxidase activity possessed by the protein (Gorecka et al., 2005). Similar analysis has shown that annBj1 of mustard also possesses peroxidase activity [Jami et al., Unpublished]. We have generated transgenic cotton expressing AnnBj1 and these plants showed enhanced tolerance to the various abiotic stress treatments like mannitol, sodium
Contents lists available at ScienceDirect
Journal of Biotechnology journal homepage: www.elsevier.com/locate/jbiotec
Abstracts
IV-3 Transgenic crops and animals
IV3-O-002 Heterologous expression of Arabidopsis Npr1 modulates insect resistance and abiotic stress tolerance also in tobacco transgenic plants T. Srinivasan, K.R. Rajesh Kumar, G. Meur, P.B. Kirti ∗ University of Hyderabad, Department of Plant Sciences, Hyderabad 500046, India E-mail address: [email protected] (P.B. Kirti). In Arabidopsis, NPR1 has been shown to modulate the salicylic acid induced systemic acquired resistance (SAR), and its biology has been reviewed extensively (Dong, 2004). It interacts with TGA factors upon nuclear localization, and enhances the expression of pathogenesis related proteins. Constitutive expression of AtNPR1 in Arabidopsis and heterologous systems like tomato and rice has been shown to enhance the plant’s resistance to diseases (Cao et al., 1998; Friedrich et al., 2001; Lin et al., 2004). We have demonstrated that the overexpression of AtNPR1 in transgenic tobacco has enhanced the host plant’s resistance against generalist herbivore, Spodoptera litura, at least to the early instars (Meur et al., 2008). High expression lines of transgenic tobacco also exhibited enhanced tolerance to abiotic and oxidative stress treatments and the tolerance was associated with constitutive upregulation of transcripts for pathogenesis related proteins like PR1, PR2 (glucanase) and PR5 (thaumatin like protein) along with proteins involved in abiotic stress like ascorbate peroxidase, Cu/Zn superoxide dismutase. Constitutive expression of PR1 has been shown to enhance abiotic stress tolerance earlier (Sarowar et al., 2005). Our studies demonstrate that constitutive expression of NPR1 enhances the plant’s overall ability to counter the various stresses, biotic as well as abiotic. Hence, it is a very valuable gene for deployment in transgenic crops.
References Cao, H., Li, X., Dong, X., 1998. Generation of broad-spectrum disease resistance by overexpression of an essential regulatory gene in systemic acquired resistance. Proc. Natl. Acad. Sci. U.S.A. 95, 6531–6536. Dong, X., 2004. NPR1, All things considered. Curr. Opin. Plant Biol. 7, 547–552. Friedrich, L., Lawton, K., Dietrich, R., Willits, M., Cade, R., Ryals, J., 2001. NIM1 overexpression in Arabidopsis potentiates plant disease resistance and results in enhanced effectiveness of fungicides. Mol. Plant Microbe Interact. 14, 1114–1124. Lin, W.C., Lu, C.F., Wu, J.W., Cheng, M.L., Lin, Y.M., Yang, N.S., Black, L., Green, S.K., Wang, J.F., Cheng, C.P., 2004. Transgenic tomato plants expressing the Arabidopsis
0168-1656/$ – see front matter
NPR1 gene display enhanced resistance to a spectrum of fungal and bacterial diseases. Transgenic Res. 13, 567–581. Meur, G., Budatha, M., Srinivasan, T., Rajesh Kumar, K.R., Datta.Gupta, A., Kirti, P.B., 2008. Overexpression of Arabidopsis NPR1 in tobacco confers enhanced resistance to the early instars of Spodoptera litura. Physiol. Plant. [Epub ahead of print] PMID: 18397206. Sarowar, S., Kim, Y.J., Kim, E.N., Kim, K.D., Hwang, B.K., Islam, R., Shin, J.S., 2005. Overexpression of a pepper basic pathogenesis-related protein-1 gene in tobacco plants enhances resistance to heavy metals and pathogen stress. Plant Cell Rep. 24, 216–224.
doi:10.1016/j.jbiotec.2008.07.501 IV3-O-003 Expression of a mustard annexin confers abiotic stress tolerance and improved fiber quality in transgenic cotton K. Divya, P.B. Kirti ∗ University of Hyderabad, Department of Plant Sciences, Hyderabad 500046, India E-mail address: [email protected] (P.B. Kirti). Cotton is a high priority commercial crop and its fiber quality is dependent upon the expression of genes like sucrose phosphate synthase, sucrose synthase and cellulose synthase. Upregulation of sucrose phosphate synthase enhanced fiber quality in normal conditions (Haigler et al., 2007). Annexins belong to a multigene family of Ca2+ dependent, phospholipid and cytoskeleton binding proteins and play a role in regulating plant responses to various abiotic stress conditions (Cantero et al., 2006). Insertion mutants of annAt1 of Arabidopsis are hypersensitive to osmotic stress and ABA during seed germination and the seedling growth indicating that annAt1 acts in a narrow developmental window at the early stages of the plant growth (Lee et al., 2004). Annexins may also serve in anchoring the accessory proteins such as sucrose synthase converting sucrose into fructose and UDP-glucose in the cotton fiber plasma membrane (Delmer and Pothika, 1997) and the implication of AnnGh1 has been suggested in cellulose synthase complex. AnnAt1 has been shown to have a potential role in scavenging free radicals developed under stress, because of the peroxidase activity possessed by the protein (Gorecka et al., 2005). Similar analysis has shown that annBj1 of mustard also possesses peroxidase activity [Jami et al., Unpublished]. We have generated transgenic cotton expressing AnnBj1 and these plants showed enhanced tolerance to the various abiotic stress treatments like mannitol, sodium