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Heterotopic bone formation by autologous osteogenic cells and growthfactors
Int. J. Oral Maxillofac. Surg. 2 0 0 5 ; 34 ( S u p p l e m e n t 1): $ 1 - $ 1 8 1
124 round and the edge of the hole was sharp. The histologically examination revealed no or little osteoid in the groups 1 and 2, whereas the group 3 samples had little or moderate new bone formation. Radiological or histological evaluation do not expose clear benefit of using PLDLA scaffolds combined with aFGF on the healing of CSDs in rats, rather it can be retarding to bone healing.
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ASSESSMENT OF THE EFFECT OF A STATIC MAGNETIC FIELD ON THE BONE HEALING: AN EXPERIMENTAL STUDY IN RATS
E. Puricelli, L. Ulbrich, D. Ponzoni, M.E. Kenner. Federal University of
Rio Grande do Sul, School of Dentistry, Oral and Maxillofacial Surgery Department, Porto Alegre, RS, Brazil To study the quality of bone healing under the effect of a permanent magnetic field, buried, "in vivo". A metallic model was created, made up of two stainless steel washers, each one attached to the bone structure by means of a commercially pure titanium screw. Twenty four rats were selected and randomically divided into five groups, from which four were test and one was control. Each animal was submitted to surgery consisting in the fixation of a pair of metallic devices on the left femur, lightly touching a surgically created bone cavity. In the test groups, the washers were set in order to apply mutual attraction forces. The animals were sacrificed after fifteen, thirty, forty-five and sixty days following the operation. The pieces were submitted to histological evaluation. Among the fifteen and thirty-day groups, the test groups showed up more accelerated healing in relation to control groups. After forty-five days, both groups presented little dissimilar results between them. After sixty days, there was marked bone neoformation in the test group, proposing a continuing magnetic stimulation effect throughout the experimental period. The magnetic stainless steel alloy, buried, in vivo, was able to stimulate and accelerate the bone healing process.
•0"-•
VARIOUS INDICATIONS FOR A NON ANIMAL, NON HUMAN REGENERATIVE GRAFT MATERIAL
B. Tomasetti 1 , R. Ewers 2. 1Private Practice Rocky Mountain Oral and Maxillofacial Surgery Littleton, CO, USA; 2University Hospital of Cranio-Maxillofacial and Oral Surgery Vienna, Austria Patients want a non human, non bovine bone graft material that id dependable and predictable. A naturally porus, natural calcium phosphate harvested from red marine algae is now available in the US as a bone replacement material The use of a dependable, predictable non human, no bovine graft material allows the placement of dental implants in areas which were previously unavailable. Patients want products and materials that have been proven to be safe and efficacious with a good long term prognosis. C-Graft, a non human, non bovine material has proven to be a dependable and predictable regenerative graft material.
~-~]
COMPARATIVE STUDY OF DIFFERENT WAYS OF OBTAINING THE PLATELET RICH PLASMA (PRP)
A. Chaves, R. Beltrao, G.C. Beltrao, G.G. Fritscher, T.D. Valcanaia.
Department of Surgery, Pontificia Universidade Catoilca RS, Porto Alegre, Brazil The objective of this study was to see the advantages of using the platelet rich plasma (PRP) in the healing of mandibular fractures, associated to the standard treatment in our hospital. This was a trial study, randomized, double blind. There were 2 separate groups - 5 persons each - one received the standard treatment and the other had the standard treatment plus the PRP. Were used panoramic radiographs and computadorized tomography images before the surgery, after the surgery, 1 month after, and 3 months after as control. Just before the surgery a 50 ml of blood was collected from the patient and divided in a 5 ml vaccuntainer tube with 0.5 ml of trisodium citratel0%.The platelet rich plasma was obtain after a eight minutes centrifugation, with 1200rpm. A 1 ml volume of PRP was then collected, in a total of 5 ml. This was activated before the application with a 10% calcium chloride. The previous results of the images examination show us a better healing in the group where was used the platelet rich plasma. In this study we can see the great use of new techniques to accelerate the bone healing. The incorporation of this
technique may bring patient benefit without risk of infection or disease transmition ~
CLINICAL AND HISTOLOGICAL EVALUATION OF THE ASSOCIATION BETWEEN BOVINE ORGANIC OSSEOUS MATRIX AND BONE MORPHOGENETIC PROTEIN (BMP) IN MAXILLARY SINUS AUGMENTATION
R. Mazzonetto, F. Serra e Silva, J.R.A. Barbosa. Piracicaba Dental
Scholl University of Campinas, Brazil The present study aims to evaluate clinically and histologically the behavior of the association between bovine organic osseous matrix and bone morphogenetic protein (BMP), when compared with autogenous bone graft in bilateral maxillary sinus lift procedures Ten non-smoking and systemically healthy patients who needed bilateral maxillary sinus grafting for the placement of osseointegrated implants were operated. In all patients, one side was selected as test and the other as control. In a second surgical intervention, the implant placement was preceded by biopsy of the lateral sinus wall using with a 2 mm trefine bur, in the region of the bone wall created in the sinus lift procedure. The specimens were stored in 10% formalin and taken to laboratorial processing for hematoxicilin-eosin dying and section preparation. The histological analysis was performed through optical microscopy. Clinically, a better bone formation pattern could be observed in the side grafted with autogenous bone. Histologically, the bone neoformation pattern was different in both sides. In the present study, the association with BMP as a growth factor showed different results when compared to autogenous graft. With the present study, it was possible to conclude that clinically the bone formation pattern is better when autogenous bone is used. Histologically, less compact and less organized bone pattern than with the autogenous bone were found. ~
HETEROTOPIC BONE FORMATION BY AUTOLOGOUS OSTEOGENIC CELLS AND GROWTHFACTORS
P. Arpornmaeklong 1 , P. Pripatnanont 1 , W. Kittidamkerng 1 , K. Wuerzler 2 .
1Oral and Maxillofacial Surgery Department, Faculty of Dentistry, Prince of Songkla University, Songkhla, and The Cranio-Maxillofacial Surgery, Thailand; 2 The University of Wuerzburg, Wuerzburg, Germany The study aims to develop an ex vivo model for induction of ectopic bone formation using autologous bone marrow cells and growth factors in an immunologically competent rat model. Under general anesthesia blood and bone marrow was collected from eighteen male Spraque-Dawly rats (300-350 grams). Autologous growth factors (GFs) were obtained from activated platelet concentrate (PC) and platelet poor plasma (PPP). Bone marrow was cultivated in osteogenic culture medium. The study was categorized into 3 groups, Group A: Cultivation of BMSCs in 2nd passage for 30 days and detecting expression of ALP activity and in vitro mineralization (10 rats); Group B: Implantation of differentiated BMSCs, 1 × 105 cells, seeded on insoluble collagenous bovine bone matrix (ICBM) supplemented with GFs from PC or PPP (5 rats); Groups C: Implantation of scaffolds without cells supplemented with PC or PPP (3 rats). The samples were intramuscularly implanted into the right (PC) and left tight muscles (PPP) of autologous hosts for 4 weeks. Amount of new bone formation was measured using NIH image analysis software. ALP activity and amount of bone formation were compared using repeated measure ANOVA and pair t-Test, respectively at p < 0.05. ALP activity reached a maximum level on days 5 and 10. In vitro mineralization of cells was clearly demonstrated in all samples. In Group B, islands of woven bone were found in 80% of samples. Amount of new bone formation in the right (0.085±0.011 inch2) and left tight muscles (0.022±0.001 inch2) of rats was not significantly different (p = 0.064). New bone formation was not found in Group C. BMSCs of adult rats could differentiate into osteoblastlike cells in vitro and form bone in vivo. A rat model of bone marrow harvesting and implantation of autologous differentiated BMSCs and growth factors in an immunologically competent host was established. ~
MAXILLARY SINUS AUGMENTATION WITH HOMOLOGOUS FROZEN BONE
A. Viscioni, M. Franco, G. Ferronato. U.O. Maxillofacial Surgery
Castelfranco Veneto (Treviso) School of Maxillofacial Surgery University of Padova, Italy The search for ideal grafting material alternative to autologous bone, for the implant-prosthetic rehabilitation of the jaws, brought us consider
124 round and the edge of the hole was sharp. The histologically examination revealed no or little osteoid in the groups 1 and 2, whereas the group 3 samples had little or moderate new bone formation. Radiological or histological evaluation do not expose clear benefit of using PLDLA scaffolds combined with aFGF on the healing of CSDs in rats, rather it can be retarding to bone healing.
•
ASSESSMENT OF THE EFFECT OF A STATIC MAGNETIC FIELD ON THE BONE HEALING: AN EXPERIMENTAL STUDY IN RATS
E. Puricelli, L. Ulbrich, D. Ponzoni, M.E. Kenner. Federal University of
Rio Grande do Sul, School of Dentistry, Oral and Maxillofacial Surgery Department, Porto Alegre, RS, Brazil To study the quality of bone healing under the effect of a permanent magnetic field, buried, "in vivo". A metallic model was created, made up of two stainless steel washers, each one attached to the bone structure by means of a commercially pure titanium screw. Twenty four rats were selected and randomically divided into five groups, from which four were test and one was control. Each animal was submitted to surgery consisting in the fixation of a pair of metallic devices on the left femur, lightly touching a surgically created bone cavity. In the test groups, the washers were set in order to apply mutual attraction forces. The animals were sacrificed after fifteen, thirty, forty-five and sixty days following the operation. The pieces were submitted to histological evaluation. Among the fifteen and thirty-day groups, the test groups showed up more accelerated healing in relation to control groups. After forty-five days, both groups presented little dissimilar results between them. After sixty days, there was marked bone neoformation in the test group, proposing a continuing magnetic stimulation effect throughout the experimental period. The magnetic stainless steel alloy, buried, in vivo, was able to stimulate and accelerate the bone healing process.
•0"-•
VARIOUS INDICATIONS FOR A NON ANIMAL, NON HUMAN REGENERATIVE GRAFT MATERIAL
B. Tomasetti 1 , R. Ewers 2. 1Private Practice Rocky Mountain Oral and Maxillofacial Surgery Littleton, CO, USA; 2University Hospital of Cranio-Maxillofacial and Oral Surgery Vienna, Austria Patients want a non human, non bovine bone graft material that id dependable and predictable. A naturally porus, natural calcium phosphate harvested from red marine algae is now available in the US as a bone replacement material The use of a dependable, predictable non human, no bovine graft material allows the placement of dental implants in areas which were previously unavailable. Patients want products and materials that have been proven to be safe and efficacious with a good long term prognosis. C-Graft, a non human, non bovine material has proven to be a dependable and predictable regenerative graft material.
~-~]
COMPARATIVE STUDY OF DIFFERENT WAYS OF OBTAINING THE PLATELET RICH PLASMA (PRP)
A. Chaves, R. Beltrao, G.C. Beltrao, G.G. Fritscher, T.D. Valcanaia.
Department of Surgery, Pontificia Universidade Catoilca RS, Porto Alegre, Brazil The objective of this study was to see the advantages of using the platelet rich plasma (PRP) in the healing of mandibular fractures, associated to the standard treatment in our hospital. This was a trial study, randomized, double blind. There were 2 separate groups - 5 persons each - one received the standard treatment and the other had the standard treatment plus the PRP. Were used panoramic radiographs and computadorized tomography images before the surgery, after the surgery, 1 month after, and 3 months after as control. Just before the surgery a 50 ml of blood was collected from the patient and divided in a 5 ml vaccuntainer tube with 0.5 ml of trisodium citratel0%.The platelet rich plasma was obtain after a eight minutes centrifugation, with 1200rpm. A 1 ml volume of PRP was then collected, in a total of 5 ml. This was activated before the application with a 10% calcium chloride. The previous results of the images examination show us a better healing in the group where was used the platelet rich plasma. In this study we can see the great use of new techniques to accelerate the bone healing. The incorporation of this
technique may bring patient benefit without risk of infection or disease transmition ~
CLINICAL AND HISTOLOGICAL EVALUATION OF THE ASSOCIATION BETWEEN BOVINE ORGANIC OSSEOUS MATRIX AND BONE MORPHOGENETIC PROTEIN (BMP) IN MAXILLARY SINUS AUGMENTATION
R. Mazzonetto, F. Serra e Silva, J.R.A. Barbosa. Piracicaba Dental
Scholl University of Campinas, Brazil The present study aims to evaluate clinically and histologically the behavior of the association between bovine organic osseous matrix and bone morphogenetic protein (BMP), when compared with autogenous bone graft in bilateral maxillary sinus lift procedures Ten non-smoking and systemically healthy patients who needed bilateral maxillary sinus grafting for the placement of osseointegrated implants were operated. In all patients, one side was selected as test and the other as control. In a second surgical intervention, the implant placement was preceded by biopsy of the lateral sinus wall using with a 2 mm trefine bur, in the region of the bone wall created in the sinus lift procedure. The specimens were stored in 10% formalin and taken to laboratorial processing for hematoxicilin-eosin dying and section preparation. The histological analysis was performed through optical microscopy. Clinically, a better bone formation pattern could be observed in the side grafted with autogenous bone. Histologically, the bone neoformation pattern was different in both sides. In the present study, the association with BMP as a growth factor showed different results when compared to autogenous graft. With the present study, it was possible to conclude that clinically the bone formation pattern is better when autogenous bone is used. Histologically, less compact and less organized bone pattern than with the autogenous bone were found. ~
HETEROTOPIC BONE FORMATION BY AUTOLOGOUS OSTEOGENIC CELLS AND GROWTHFACTORS
P. Arpornmaeklong 1 , P. Pripatnanont 1 , W. Kittidamkerng 1 , K. Wuerzler 2 .
1Oral and Maxillofacial Surgery Department, Faculty of Dentistry, Prince of Songkla University, Songkhla, and The Cranio-Maxillofacial Surgery, Thailand; 2 The University of Wuerzburg, Wuerzburg, Germany The study aims to develop an ex vivo model for induction of ectopic bone formation using autologous bone marrow cells and growth factors in an immunologically competent rat model. Under general anesthesia blood and bone marrow was collected from eighteen male Spraque-Dawly rats (300-350 grams). Autologous growth factors (GFs) were obtained from activated platelet concentrate (PC) and platelet poor plasma (PPP). Bone marrow was cultivated in osteogenic culture medium. The study was categorized into 3 groups, Group A: Cultivation of BMSCs in 2nd passage for 30 days and detecting expression of ALP activity and in vitro mineralization (10 rats); Group B: Implantation of differentiated BMSCs, 1 × 105 cells, seeded on insoluble collagenous bovine bone matrix (ICBM) supplemented with GFs from PC or PPP (5 rats); Groups C: Implantation of scaffolds without cells supplemented with PC or PPP (3 rats). The samples were intramuscularly implanted into the right (PC) and left tight muscles (PPP) of autologous hosts for 4 weeks. Amount of new bone formation was measured using NIH image analysis software. ALP activity and amount of bone formation were compared using repeated measure ANOVA and pair t-Test, respectively at p < 0.05. ALP activity reached a maximum level on days 5 and 10. In vitro mineralization of cells was clearly demonstrated in all samples. In Group B, islands of woven bone were found in 80% of samples. Amount of new bone formation in the right (0.085±0.011 inch2) and left tight muscles (0.022±0.001 inch2) of rats was not significantly different (p = 0.064). New bone formation was not found in Group C. BMSCs of adult rats could differentiate into osteoblastlike cells in vitro and form bone in vivo. A rat model of bone marrow harvesting and implantation of autologous differentiated BMSCs and growth factors in an immunologically competent host was established. ~
MAXILLARY SINUS AUGMENTATION WITH HOMOLOGOUS FROZEN BONE
A. Viscioni, M. Franco, G. Ferronato. U.O. Maxillofacial Surgery
Castelfranco Veneto (Treviso) School of Maxillofacial Surgery University of Padova, Italy The search for ideal grafting material alternative to autologous bone, for the implant-prosthetic rehabilitation of the jaws, brought us consider