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High glucose activates claudin-1 and filaggrin expression: Possible mechanism of the antiinflammatory effect by repairing the skin barrier
Abstracts from the 41st Annual Meeting / Journal of Dermatological Science 86 (2017) e1–e95
P05-21[O2-33] The recovery rate of skin temperature after cold stress but not blood flow in a resting state is related to dry skin conditions Yasuko Amano 1,∗ , Tomoko Nomura 2 , Yoshinori Sugiyama 1 , Kayoko Iwata 3 , Yuko Higaki 4 , Masanori Tanahashi 1 1 Health Beauty Products, Kao Corporation, Tokyo, Japan 2 Biological Science Laboratory, Kao Corporation, Tochigi, Japan 3 Lifestyle Research Center, Kao Corporation, Tokyo, Japan 4 Institute of Women’s Health, Tokyo Women’s Medical University, Tokyo, Japan Cutaneous blood flow plays important roles in thermoregulation, oxygen supply and nutritional support which are necessary to maintain the skin. However, there is little evidence for a link between blood flow and skin physiology. In this study, skin parameters which relate to dry skin conditions were assessed in relation to blood flow in the resting state and blood vessel functionality measured by cold-stress test (CST). The relationships between vascular and dry skin parameters were studied in 56 female volunteers. The recovery rate (RR) of skin temperature after a CST showed a significant negative correlation with visual dryness scores in skin on the leg and was positively correlated with capacitance in skin on the arm. These significant correlations remained when corrected for the effect of age. The involvement of the RR in seasonal changes of dry skin conditions was assessed in 37 female volunteers. The participants were divided into two groups; high RR (>70%) and low RR (<70%). In the cheek and leg, deterioration in skin dryness from summer to winter was significant in the low RR group. To clarify the relationship between RR and facial blood flow under cold stimuli, a local cooling test was carried out on 18 male volunteers. The relative blood flow during local facial cooling was positively correlated with RR, indicating that RR represents the functionality of facial blood flow under cold stimuli. These results suggest that the RR but not blood flow at the resting state is associated with dry skin condition and may be involved in skin homeostasis during seasonal environmental changes.
http://dx.doi.org/10.1016/j.jdermsci.2017.02.137 P05-22[O2-34] Relationships between transepidermal water loss, cutaneous microcirculatory function and autonomic nerve activity Tomoko Nomura 1,∗ , Yasuko Amano 2 , Kenichiro Yoshida 2 , Akihiko Fujii 1 , Masanori Tanahashi 2 , Yoshinori Sugiyama 2 , Kayoko Iwata 3 , Takatoshi Murase 1 1
Biological Science Laboratory, Kao Corporation, Tochigi, Japan 2 Health Beauty Products, Kao Corporation, Tokyo, Japan 3 Lifestyle Research Center, Kao Corporation, Tokyo, Japan Autonomic nerve system and cardiovascular system are important to maintain tissue homeostasis. In cutaneous tissue, these two systems play crucial role and several reports suggest that skin properties are associated with cutaneous microcirculatory function and
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autonomic nerve activity. However, it has not yet been elucidated in detail how these factors are correlated with skin properties. In this study, the relationships between skin properties, cutaneous microcirculatory function and autonomic nerve activity were investigated in 19 healthy adult male volunteers. The hydration of the stratum corneum and transepidermal water loss (TEWL) were investigated as skin properties, and the responsiveness of skin blood flow (SkBF) to local warming was examined as an index of cutaneous microcirculatory function. Electrocardiograms were monitored for 24 h and heart rate variability was analyzed as low frequency power (LF: 0.04–0.15 Hz), high frequency power (HF: 0.15–0.40 Hz) and as a ratio of low-to-high frequency power (LF/HF) as indices of autonomic nerve activity; HF for parasympathetic activity, LF/HF for sympathovagal balance. The relationships between those indices were then analyzed. A significant negative correlation was found between TEWL and the relative maximum rate of increases in SkBF (SlopeMAX%) on local warming. Significant positive correlations were observed between TEWL and LF/HF and between HF and SlopeMAX%. The hydration of the stratum corneum did not show significant correlations with any indices of microcirculation or autonomic nerve activity. These results suggest that skin barrier function, cutaneous microcirculatory function and autonomic nerve activity might interact in healthy adults. http://dx.doi.org/10.1016/j.jdermsci.2017.02.138 P05-23[O2-35] High glucose activates claudin-1 and filaggrin expression: Possible mechanism of the antiinflammatory effect by repairing the skin barrier Kiyoko Yamada The Department of Dermatology, Kagoshima University Graduate School of Medicine and Dental Science, Japan Background: Topical application of sugars has been known to be effective for wounded skin. Recently, we demonstrated that topical application of high glucose improves skin inflammation in mite antigen-sensitized NC/Nga mice. However, the underlying mechanism has remained to be elucidated. Objective: To try to determine the mechanism by which high glucose improves skin inflammation, we examined the effect of high glucose on the expression of claudin-1 and filagrrin, and on the disease severity and cytokine levels in model mice sensitized by mite antigen. Methods: Expression of claudin-1 and filagrrin induced by stimulation with high glucose (55 mM) were determined by Western blotting and RT-PCR in cultured human skin keratinocytes and mite antigen-sensitized NC/Nga mice. Skin inflammation in the ears were evaluated by skin severity scores, ear thickness and histological findings. http://dx.doi.org/10.1016/j.jdermsci.2017.02.139
P05-21[O2-33] The recovery rate of skin temperature after cold stress but not blood flow in a resting state is related to dry skin conditions Yasuko Amano 1,∗ , Tomoko Nomura 2 , Yoshinori Sugiyama 1 , Kayoko Iwata 3 , Yuko Higaki 4 , Masanori Tanahashi 1 1 Health Beauty Products, Kao Corporation, Tokyo, Japan 2 Biological Science Laboratory, Kao Corporation, Tochigi, Japan 3 Lifestyle Research Center, Kao Corporation, Tokyo, Japan 4 Institute of Women’s Health, Tokyo Women’s Medical University, Tokyo, Japan Cutaneous blood flow plays important roles in thermoregulation, oxygen supply and nutritional support which are necessary to maintain the skin. However, there is little evidence for a link between blood flow and skin physiology. In this study, skin parameters which relate to dry skin conditions were assessed in relation to blood flow in the resting state and blood vessel functionality measured by cold-stress test (CST). The relationships between vascular and dry skin parameters were studied in 56 female volunteers. The recovery rate (RR) of skin temperature after a CST showed a significant negative correlation with visual dryness scores in skin on the leg and was positively correlated with capacitance in skin on the arm. These significant correlations remained when corrected for the effect of age. The involvement of the RR in seasonal changes of dry skin conditions was assessed in 37 female volunteers. The participants were divided into two groups; high RR (>70%) and low RR (<70%). In the cheek and leg, deterioration in skin dryness from summer to winter was significant in the low RR group. To clarify the relationship between RR and facial blood flow under cold stimuli, a local cooling test was carried out on 18 male volunteers. The relative blood flow during local facial cooling was positively correlated with RR, indicating that RR represents the functionality of facial blood flow under cold stimuli. These results suggest that the RR but not blood flow at the resting state is associated with dry skin condition and may be involved in skin homeostasis during seasonal environmental changes.
http://dx.doi.org/10.1016/j.jdermsci.2017.02.137 P05-22[O2-34] Relationships between transepidermal water loss, cutaneous microcirculatory function and autonomic nerve activity Tomoko Nomura 1,∗ , Yasuko Amano 2 , Kenichiro Yoshida 2 , Akihiko Fujii 1 , Masanori Tanahashi 2 , Yoshinori Sugiyama 2 , Kayoko Iwata 3 , Takatoshi Murase 1 1
Biological Science Laboratory, Kao Corporation, Tochigi, Japan 2 Health Beauty Products, Kao Corporation, Tokyo, Japan 3 Lifestyle Research Center, Kao Corporation, Tokyo, Japan Autonomic nerve system and cardiovascular system are important to maintain tissue homeostasis. In cutaneous tissue, these two systems play crucial role and several reports suggest that skin properties are associated with cutaneous microcirculatory function and
e47
autonomic nerve activity. However, it has not yet been elucidated in detail how these factors are correlated with skin properties. In this study, the relationships between skin properties, cutaneous microcirculatory function and autonomic nerve activity were investigated in 19 healthy adult male volunteers. The hydration of the stratum corneum and transepidermal water loss (TEWL) were investigated as skin properties, and the responsiveness of skin blood flow (SkBF) to local warming was examined as an index of cutaneous microcirculatory function. Electrocardiograms were monitored for 24 h and heart rate variability was analyzed as low frequency power (LF: 0.04–0.15 Hz), high frequency power (HF: 0.15–0.40 Hz) and as a ratio of low-to-high frequency power (LF/HF) as indices of autonomic nerve activity; HF for parasympathetic activity, LF/HF for sympathovagal balance. The relationships between those indices were then analyzed. A significant negative correlation was found between TEWL and the relative maximum rate of increases in SkBF (SlopeMAX%) on local warming. Significant positive correlations were observed between TEWL and LF/HF and between HF and SlopeMAX%. The hydration of the stratum corneum did not show significant correlations with any indices of microcirculation or autonomic nerve activity. These results suggest that skin barrier function, cutaneous microcirculatory function and autonomic nerve activity might interact in healthy adults. http://dx.doi.org/10.1016/j.jdermsci.2017.02.138 P05-23[O2-35] High glucose activates claudin-1 and filaggrin expression: Possible mechanism of the antiinflammatory effect by repairing the skin barrier Kiyoko Yamada The Department of Dermatology, Kagoshima University Graduate School of Medicine and Dental Science, Japan Background: Topical application of sugars has been known to be effective for wounded skin. Recently, we demonstrated that topical application of high glucose improves skin inflammation in mite antigen-sensitized NC/Nga mice. However, the underlying mechanism has remained to be elucidated. Objective: To try to determine the mechanism by which high glucose improves skin inflammation, we examined the effect of high glucose on the expression of claudin-1 and filagrrin, and on the disease severity and cytokine levels in model mice sensitized by mite antigen. Methods: Expression of claudin-1 and filagrrin induced by stimulation with high glucose (55 mM) were determined by Western blotting and RT-PCR in cultured human skin keratinocytes and mite antigen-sensitized NC/Nga mice. Skin inflammation in the ears were evaluated by skin severity scores, ear thickness and histological findings. http://dx.doi.org/10.1016/j.jdermsci.2017.02.139