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Histochemical study of pulp healing
HISTOCHEMICAL
STUDY
OF PULP HEALING
R. L. Cabrini, M.D., 0. A. Maisto, D.D.S., and E. E. Manfredi, Buenos Aires, Argentina
T
D.D.S.,
action of alkaline phosphatase, mucopolysaccharides, and glycogen in the prowess of pulp healing has been studied. For this purpose, healthy or normal pulp of fifteen human teeth has been used. The pulp chambers of five teeth were perforated and capped with calcium hydroxide; five other teeth were also perforated and capped with zinc oxide-eugenol; and the last five normal teeth were kept as untreated controls. The teeth were extracted after fifteen and thirty days. During this period the new dentine starts to form in the teeth treated with calcium hydroxide.‘, 2 After the tooth was fractured, the pulp was extracted and fixed in 80 per cent alcohol at 4” C. It was embedded in paraffin, and alkaline phosphatase was demonstrated by Gomori’s method; mucopolysaccharides were demonstrated by the PAS method of McManus. Controls were treated with ptyalin. The alkaline phosphatase reaction of pulp wounds increases slightly. No differences were detected between teeth treated with calcium hydroxide and those treated with zinc oxide-eugenol.3T4 On the other hand, all teeth treated with calcium hydroxide showed large amounts of PAS-positive granules in newly formed odontoblasts, preodontoblasts, and pulp cells of the healing and adjacent areas. No changes were seen in control teeth or in teeth treated with zinc oxideeugenol. These PAS-positive granules were removed by ptyalin, which showed them to be glycogen. HE
SUMMARY
The results of this study indicate that the histochemical demonstration of glycogen gives useful evidence of pulp healing if we admit that most pulps treated with calcium hydroxide heal with new formation of dentine, whereas those treated with zinc oxide-eugenol do not.l* 2,6 On the other hand, these results confirm the importance that glycogen seems to have in the formation of tissues with a calcified matrix.6 REFERENCES
1. Cabrini, R. L., M&to, 293, 1953. From Argentina.
the
Laboratorio
0. A., and Manfredi, de Anatomfa
Patol6gica
868
E. E.:
Rev. odont.
Hospital
Ramos
( Buenos Mejk,
Aires)
Buenos
41: Aires.
Volume 13 Number 7
2. Cabrini, 3. 4. 5. 6.
HISTOCHEMICAL
STUDY
OF PULP
HEALING
R. L., Maisto, 0. A., and Manfredi, E. E.: Rev. Asoc. odont. argent. 1956. Cabrini, R. I,.: Rev. odont. (Buenos Aires) 38: 316, 1950. Cabrini, R. L., and Manfredi, E. E.: Rev. Asoc. odont.. argent. 46: 409, 1958. Nyborg, H.: Odontol. Tidsckr. 66: 296, 1958. Schajowicz, F., and Cabrini, R. L.: J. Bone & Joint Burg. 40a: 1081, 1958.
Pm 44: 4%.
STUDY
OF PULP HEALING
R. L. Cabrini, M.D., 0. A. Maisto, D.D.S., and E. E. Manfredi, Buenos Aires, Argentina
T
D.D.S.,
action of alkaline phosphatase, mucopolysaccharides, and glycogen in the prowess of pulp healing has been studied. For this purpose, healthy or normal pulp of fifteen human teeth has been used. The pulp chambers of five teeth were perforated and capped with calcium hydroxide; five other teeth were also perforated and capped with zinc oxide-eugenol; and the last five normal teeth were kept as untreated controls. The teeth were extracted after fifteen and thirty days. During this period the new dentine starts to form in the teeth treated with calcium hydroxide.‘, 2 After the tooth was fractured, the pulp was extracted and fixed in 80 per cent alcohol at 4” C. It was embedded in paraffin, and alkaline phosphatase was demonstrated by Gomori’s method; mucopolysaccharides were demonstrated by the PAS method of McManus. Controls were treated with ptyalin. The alkaline phosphatase reaction of pulp wounds increases slightly. No differences were detected between teeth treated with calcium hydroxide and those treated with zinc oxide-eugenol.3T4 On the other hand, all teeth treated with calcium hydroxide showed large amounts of PAS-positive granules in newly formed odontoblasts, preodontoblasts, and pulp cells of the healing and adjacent areas. No changes were seen in control teeth or in teeth treated with zinc oxideeugenol. These PAS-positive granules were removed by ptyalin, which showed them to be glycogen. HE
SUMMARY
The results of this study indicate that the histochemical demonstration of glycogen gives useful evidence of pulp healing if we admit that most pulps treated with calcium hydroxide heal with new formation of dentine, whereas those treated with zinc oxide-eugenol do not.l* 2,6 On the other hand, these results confirm the importance that glycogen seems to have in the formation of tissues with a calcified matrix.6 REFERENCES
1. Cabrini, R. L., M&to, 293, 1953. From Argentina.
the
Laboratorio
0. A., and Manfredi, de Anatomfa
Patol6gica
868
E. E.:
Rev. odont.
Hospital
Ramos
( Buenos Mejk,
Aires)
Buenos
41: Aires.
Volume 13 Number 7
2. Cabrini, 3. 4. 5. 6.
HISTOCHEMICAL
STUDY
OF PULP
HEALING
R. L., Maisto, 0. A., and Manfredi, E. E.: Rev. Asoc. odont. argent. 1956. Cabrini, R. I,.: Rev. odont. (Buenos Aires) 38: 316, 1950. Cabrini, R. L., and Manfredi, E. E.: Rev. Asoc. odont.. argent. 46: 409, 1958. Nyborg, H.: Odontol. Tidsckr. 66: 296, 1958. Schajowicz, F., and Cabrini, R. L.: J. Bone & Joint Burg. 40a: 1081, 1958.
Pm 44: 4%.