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Histologic analysis of two dental implants extracted after osseointegration
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d e n t a l m a t e r i a l s 3 1 S ( 2 0 1 5 ) e1–e66
92
93
OCT evaluation of marginal seal of bulk-fill composites in dentin
Histologic analysis of two dental implants extracted after osseointegration
A. Comba 1,∗ , G. Ventura 1 , A. Luganini 3 , M. Alovisi 1 , C. Alovisi 2 , E. Berutti 1 , N. Scotti 1
V. Checchi 1,∗ , L. Checchi 2 , A. Mazzoni 2 , L. Breschi 2 , P. Felice 2
1
1
University of Turin, Department of Surgical Sciences, Dental School, Italy 2 University of Turin, Department of Surgical Sciences, Italy 3 Department of Life Sciences and Systems Biology, University of Turin, Italy Purpose: The aim of this in vitro study was a non-invasive evaluation of the marginal sealing to dentin cervical margin of second class cavities after thermal aging using the optical coherence tomography (OCT). The null hypothesis is that there is no difference in marginal sealing between composites of different viscosity. Methods and materials: 8 intact upper premolars, extracted for periodontal reasons, were selected. A mesiooccluso-distal cavity with cervical margins placed 1 mm below the CEJ was performed on each tooth. Samples were then all treated with Scotchbond Universal in etch-and-dry technique. Restoration was performed as follows: a 1 mm horizontal layer of bulk-fill composite (Venus Bulk-Fill, Heraeus Kulzer) was placed over only the mesio-cervical cavity floor and cured for 20 s. Then composite restoration of the cavity was performed with oblique layering of nanohybrid resin composite (Clearfill ES-2, Kuraray). OCT (‘Spectralis’, Heidelberg-Engineering, Germany) imaging was performed after 24 h and 2000 thermocycles. Selected samples were sectioned for interfacial observation by confocal laser scanning microscope (CLSM). Marginal adaptation (percentage) was analyzed on 20 B-scans through each sample. Images were analyzed with the software ImageJ to assess the percentage of marginal gap between composite and cervical margin. Data were statistically analyzed with ANOVA test and significance was set for p < 0.05. Results: Statistical analysis showed any differences in marginal adaptation obtained with Venus Bulk-Fill and Clearfill. Thermal cycling significantly affected marginal integrity of tested composites (p = 0.001). CLSM closely confirmed OCT findings in all samples. Conclusion: The null hypothesis is accepted. Thermal aging significantly affected sealing of composites on dentin margin. http://dx.doi.org/10.1016/j.dental.2015.08.093
2
University of Trieste, Italy University of Bologna, Italy
Purpose: The aim of this case report was to analyze and observe the bone tissue formation around two dental implants after their osseointegration. Methods and materials: A 58 y.o. woman was referred to the Division of Periodontology and Implantology, Department of Biomedical and Neuromotor Sciences (University of Bologna, Italy) due to severe pain and discomfort caused by two of the four dental implants placed in the upper jaw (sites #13 and #23) in a private practice 8 months before. Implants were made of titanium, sandblasted with zirconium oxide and etched with mineral acids (Sweden & Martina, Due Carrare, Italy). At clinical and radiographic analysis, implants appeared properly osseointegrated but placed tilted way too buccaly, with their emergence profile in non-keratinized oral mucosa. For the above-mentioned reasons, the patient started having discomfort immediately after the prosthetic load occurred four months after implants placement. The patient was rehabilitated with an implant supported removable prosthesis. The patient explains difficulties to perform adequate oral hygiene and declares pain every time she had to wear the implantsupported removable prosthesis. The implants were removed, preserving the bone around implant threads, and replaced with two new dental implants, placed in a prosthetic-guided correct inclination. The removed implants were processed for histological analysis in accordance with Checchi et al. Results: The histological analysis revealed proper osseointegration of the implant fixture after eight months. Histological views of biopsies show good bone-implant contact recorded from the coronal side to the apical implant section. Presence of direct connecting bridges between the peri-implant bone trabeculae and the implant surface were recorded. Conclusion: This case report clearly shows how after 8 months from implant placement, the fixtures coated with a rough surface were properly osseointegrated with good boneimplant contact recorded along their entire surface. http://dx.doi.org/10.1016/j.dental.2015.08.094
d e n t a l m a t e r i a l s 3 1 S ( 2 0 1 5 ) e1–e66
92
93
OCT evaluation of marginal seal of bulk-fill composites in dentin
Histologic analysis of two dental implants extracted after osseointegration
A. Comba 1,∗ , G. Ventura 1 , A. Luganini 3 , M. Alovisi 1 , C. Alovisi 2 , E. Berutti 1 , N. Scotti 1
V. Checchi 1,∗ , L. Checchi 2 , A. Mazzoni 2 , L. Breschi 2 , P. Felice 2
1
1
University of Turin, Department of Surgical Sciences, Dental School, Italy 2 University of Turin, Department of Surgical Sciences, Italy 3 Department of Life Sciences and Systems Biology, University of Turin, Italy Purpose: The aim of this in vitro study was a non-invasive evaluation of the marginal sealing to dentin cervical margin of second class cavities after thermal aging using the optical coherence tomography (OCT). The null hypothesis is that there is no difference in marginal sealing between composites of different viscosity. Methods and materials: 8 intact upper premolars, extracted for periodontal reasons, were selected. A mesiooccluso-distal cavity with cervical margins placed 1 mm below the CEJ was performed on each tooth. Samples were then all treated with Scotchbond Universal in etch-and-dry technique. Restoration was performed as follows: a 1 mm horizontal layer of bulk-fill composite (Venus Bulk-Fill, Heraeus Kulzer) was placed over only the mesio-cervical cavity floor and cured for 20 s. Then composite restoration of the cavity was performed with oblique layering of nanohybrid resin composite (Clearfill ES-2, Kuraray). OCT (‘Spectralis’, Heidelberg-Engineering, Germany) imaging was performed after 24 h and 2000 thermocycles. Selected samples were sectioned for interfacial observation by confocal laser scanning microscope (CLSM). Marginal adaptation (percentage) was analyzed on 20 B-scans through each sample. Images were analyzed with the software ImageJ to assess the percentage of marginal gap between composite and cervical margin. Data were statistically analyzed with ANOVA test and significance was set for p < 0.05. Results: Statistical analysis showed any differences in marginal adaptation obtained with Venus Bulk-Fill and Clearfill. Thermal cycling significantly affected marginal integrity of tested composites (p = 0.001). CLSM closely confirmed OCT findings in all samples. Conclusion: The null hypothesis is accepted. Thermal aging significantly affected sealing of composites on dentin margin. http://dx.doi.org/10.1016/j.dental.2015.08.093
2
University of Trieste, Italy University of Bologna, Italy
Purpose: The aim of this case report was to analyze and observe the bone tissue formation around two dental implants after their osseointegration. Methods and materials: A 58 y.o. woman was referred to the Division of Periodontology and Implantology, Department of Biomedical and Neuromotor Sciences (University of Bologna, Italy) due to severe pain and discomfort caused by two of the four dental implants placed in the upper jaw (sites #13 and #23) in a private practice 8 months before. Implants were made of titanium, sandblasted with zirconium oxide and etched with mineral acids (Sweden & Martina, Due Carrare, Italy). At clinical and radiographic analysis, implants appeared properly osseointegrated but placed tilted way too buccaly, with their emergence profile in non-keratinized oral mucosa. For the above-mentioned reasons, the patient started having discomfort immediately after the prosthetic load occurred four months after implants placement. The patient was rehabilitated with an implant supported removable prosthesis. The patient explains difficulties to perform adequate oral hygiene and declares pain every time she had to wear the implantsupported removable prosthesis. The implants were removed, preserving the bone around implant threads, and replaced with two new dental implants, placed in a prosthetic-guided correct inclination. The removed implants were processed for histological analysis in accordance with Checchi et al. Results: The histological analysis revealed proper osseointegration of the implant fixture after eight months. Histological views of biopsies show good bone-implant contact recorded from the coronal side to the apical implant section. Presence of direct connecting bridges between the peri-implant bone trabeculae and the implant surface were recorded. Conclusion: This case report clearly shows how after 8 months from implant placement, the fixtures coated with a rough surface were properly osseointegrated with good boneimplant contact recorded along their entire surface. http://dx.doi.org/10.1016/j.dental.2015.08.094