Histological techniques

ARTICLE IN PRESS Abstracts / Experimental and Toxicologic Pathology 61 (2009) 387–413

Poster Abstracts Histological techniques P01 Enhanced brain trimming in preclinical general toxicity study: Application to the rat brain Rossella Defazio, Anna Lanzoni, Pamela Rodegher, Patrizia Cristofori GlaxoSmithKline S.p.A., Pathology Department, Verona, Italy The brain is a complex organ, with brain regions showing different physiologic activities, metabolic demands or properties, sensitivities to toxicants. In the context of preclinical general toxicity studies for medicine product development, the microscopic evaluation of the brain is performed on coronal sections at three levels, to include cerebrum, cerebellum and brain stem, in accordance to the current regulatory requirements. However, in the case of substances active on the central nervous system, histopathological examination should be extended to cells or regions potentially targets for effect or toxicity. To this aim, we have developed a standardized trimming protocol for the rat brain, to assist in an extensive neuroanatomic-aware sampling. This tool allows the toxicologic pathologist in the precise identification of neuroanatomical areas and in the accurate and focused examination of the brain. This protocol can be implemented in preclinical general toxicity studies in the rat to properly address focused brain evaluation taking into account medicine product pharmacodynamic profile and/or signs observed in the in-life phase of the study. This poster presents the methodological details of this work. The research described in this paper complied with national legislation and with the company policy on the care and use of laboratory animals and with related codes of practice. doi: 10.1016/j.etp.2009.02.087

P02 Enhanced brain trimming in preclinical general toxicity study: Application to the beagle dog brain Alessandro Piaia, Anna Lanzoni, Greta Moretti, Patrizia Cristofori GlaxoSmithKline S.p.A., Pathology Department, Verona, Italy Histopathological examination of the brain of the dog from preclinical general toxicity studies is done on sections taken coronally at three levels, to include cerebrum, cerebellum and brain stem, as in the rat

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model and in accordance to the current regulatory requirements. Considering also the large size of the brain in this species, the extent of this examination could be not sufficient in case of medicine compounds potentially active on the central nervous system. To this aim analogously to the work done on the rat in our laboratory, we have developed a standardized trimming protocol for the beagle dog brain, to assist in an extensive neuroanatomic-aware sampling. This tool allows the toxicologic pathologist in the precise identification of neuroanatomical areas and in the accurate and focused examination of the brain. This protocol can be implemented in preclinical general toxicity studies in the beagle dog to properly address focused brain evaluation taking into account medicine product pharmacodynamic profile and/or signs observed in the in-life phase of the study. This poster presents the methodological details of this work. The research described in this paper complied with national legislation and with the company policy on the care and use of laboratory animals and with related codes of practice. doi: 10.1016/j.etp.2009.02.088

P03 Ocular collection protocol for the laboratory rabbit, dog, and monkey James A. Rendera, Richard R. Dubielzigb, Dale G. Dunnc, Jerry F. Hardistyd, Rebecca R. Moored, David A. Sabiod a Pfizer Global Research and Development, Groton, Connecticut, United States b Comparative Ophthalmic Research Laboratories of the University of Wisconsin School of Veterinary Medicine, Madison, Wisconsin, United States c Covance Laboratories, Inc., Vienna, Virginia, United States d Experimental Pathology Laboratories, Inc., Research Triangle Park, North Carolina, United States Ocular Collection Protocol for the Laboratory Rabbit, Dog, and Monkey is a CD-ROM, which will serve as an educational tool and resource for pathologists, technicians, pathologists-in-training, and any other individuals involved in the collection, processing, and evaluation of ocular specimens of large laboratory animals (monkey, dog, and rabbit). The three species are dealt with separately in order to address the speciesspecific differences in anatomy and ocular pathology. This CD-ROM covers various ocular methods, including ocular tissue collection, histotechnology (fixation, trimming, processing, embedding, sectioning and other aspects of slide preparation), normal macroscopic and