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New Technical Tip articles published recently in Technical Tips Online include: Cormack, R.S. and Somssich, I.E. (1997) Cloning of PCR products using the green fluorescent protein T01107
Hervieu, G. (1997) A quick and safe method for destaining Coomassie-blue-stained protein gels T01089 Papp, T., Pemsel, H., Weiss, D.G. and Schiffmann, D. (1997) Simultaneous detection of homozygous deletion and point mutations in tumor suppressor genes p16 and p53 by multiplex-PCR/SSCP analysis T01018
Topal, A., Karaer, S. and Temizkan, G. (1997) A simple method for rescuing autonomous plasmids from fission yeast T01070
Schneider, S. et al. (1997) Dual translation cassettes permitting prokaryotic and vertebrate protein expression from the same vector T01086 Fuchs, C., Liehr, T. and Rautenstrauss, B. (1997) High resolution FISH of stretched chromosome fibers T01114
Editor Adrian Bird, Institute for Cell and Molecular Biology at the University of Edinburgh
Microweb HIV resources on t h e W e b
he amount of HIV and AIDS information available on the World Wide Web is phenomenal. The vast majority of the sites relate to the social and political aspects of the disease [for example, the JAMA HIV/AIDS information center (http : / / w-w~q", ama-assn, org/ special/hiv/hivhome, htm)], but several sites are dedicated to current research on the retrovirus. Of particular interest is the 'Current virologist: HIV and AIDS research' site run by Ramu Subbramanian at the University of Montreal, Canada (http: / /
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al i z e. ERE. U M o n t r e a l . C A :8 0 / - s u b b r a m r / ) . This colourful site
summarizes recent developments from the HIV/AIDS information service (ATIS) and provides links to the home pages of popular journals that publish virology-related articles. There is also direct access to full text versions of some recently published articles of interest (e.g. those in Cell, Virology and J. Exp. Med.). In addition, abstracts from
past and future conferences are included, and there is access to The Dictionary of Cell Biology, as well as a marvellous alphabetical glossary of HIV/AIDS-related terms (especially useful to the nonspecialist browser). For the more practical aspects of HIV research, the Web also contains several databases. One of these, containing HIV proteinase structures (http: //www- fbsc.ncifcrf. gov/HIVdb), has recently been reviewed in Trends Biochem. Sci. 1 Other useful databases include the 'HIV sequence database' ( h t t p : / / hiv-web, lanl.gov) and, its companion database, the 'HIV molecular immunology database' (http: //hiv-web. lanl .gov/ immuno/), which aims to provide a comprehensive listing of defined HIV epitopes. Both databases are located at the Los Alamos National Laboratory, NM, USA.
Microbial g e n o m e s http : / / w w w . mailbase, ac. uk/ lists/microbial-genomes / 'ollowing the 'Small Genomes: Sequencing, Functional Characterization and Comparative Genomics (TIGR)' conference earlier this year 1, Mark Pallen has set up a 'microbial genomes' mailing list to keep microbiologists and molecular biologists up to date with this exciting and rapidly progressing field. The list currently includes over 350 members, and the aim is to provide a medium to discuss the progress made on genome (bacterial, archaeal, viral and small eukaryotic genomes) sequencing projects and technical details of sequencing and sequence analysis.
p
Reference
1 Ash, C. (1997)TrendsMicrobiol.5, 135-139
Reference
Coming soon!
1 Vondrasek,J. and Wlodawer,A. (1997) TrendsBiochem.Sci.22, 183
The Trends Guide to the Internet (199 7) A free guide available in the September issue of Trends in Microbiology.
Microweb was compiled by Michaela Blackford. Please send suggestions for inclusion to tlm@ellsevler,co.uk
Copyright © 1997 Elsevier Science Ltd. All rights reserved. 0966 842X/97/$17.00
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