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HLA-A, B and C frequencies in Lebanese
Abstracts
109
B 9.1.205
CELL-FREE ASSAY PREDICTS PROLIFERATIVE SUPPRESSION BY CYCLOSPORINE AND RAPAMYCIN. PM Kimball, RH Kerman, and BD Kahan, Univeristy of Texas Medical School, Houston, Texas. We report that Cyclosporine (CsA) and Rapamycin (RAPA) inhibit protein kinase C[PKC]-triggering of the intracellular activation signal ADR (Activator of DNA Replication) in a cell-free assay. Furthermore, ADR induction is dosedependent and correlates with proliferative suppression by CsA or RAPA. Peripheral blood lymphocyte cytosol was isolated and endogenous PKC triggered with 50 nM phorbol myristate acetate (PMA). ADR was subsequently measured by uptake of tritiated thymidine. PKC activation induced ADR (890+ 120 cpm v~ 3910+345, p<0.001). In contrast, PKC inhibition by H-7 blocked ADR generation (100+50 cpm vs 3910+345, p<0.001). ADR was not found following cell cultivation with PHA and 50 nM CsA (910+40 cpm with CsA vs 6410+830 cpm without CsA, p<0.001) nor was it induced by PKC (750+80 cpm). Pretreatment of unstimulated cells with CsA or RAPA blocked ADR induction by PKC. CsA doses of 10, 50 or 100 ng/ml inhibited ADR induction 10%, 59% or 73% and T-cell mitogenesis by 15%, 63% or 79%, respectively. RAPA pretreatment of resting cells resulted in similar correlations between ADR inducability versus proliferation suppression. In summary, cytosolic ADR was generated following PKC activation. Cell-free quantitation of ADR mimiced proliferative inhibition by CsA and RAPA. Thus, cell-free assay of ADR may permit monitoring of clinical immunosuppression as well as dissection of phosphorylated intermediates necessary for intracellular communication.
B 9.1.206
H L A - A , B AND C F R E Q U E N C I E S IN L E B A N E S E . M a y A b d e l n o o r and A l e x a n d e r M. A b d e l n o o r , N o t r e D a m e D e s S e c o u r s H o s p i t a l an~--~merlcan ~n-i-~ersity of B e i r u t , L e b a n o n . H L A - A , B and C f r e q u e n c i e s w e r e d e t e r m i n e d in 416 lebanese.These i n c l u d e d 237 m o s l e m s and 179 c h r i s t i a n s . A m o n g the m o s l e m s t h e r e w e r e 149 c h i i t e s and 88 s u n n i t e s . A m o n g the c h r i s t i a n s t h e r e w e r e 129 m a r o n i t e s , 2 7 o r t h o d o x and 23 c a t h o l i c s . The m e t h o d u s e d for HLA d e t e r m i n a t i o n s w a s the c o m p l e m e n t d e p e n d e n t m i c r o c y t o t o x i c i t y assay. The f r e q u e n c i e s of the v a r i o u s H L A - A , B and C d e t e r m i n e d in leba n e s e c h r i s t i a n s and m o s l e m s w e r e c o m p a r e d to e a c h o t h e r and to t h o s e of r e p o r t e d f r e q u e n c i e s for e u r o p e a n s . T h e r e s u l t s i n d i c a t e d that; i- A 2 , B 7 and B27 o c c u r r e d m o r e f r e q u e n t l y in m o s l e m s t h a n in c h r i s t i a n s . 2- A 9 , B 3 5 and Cw4 o c c u r r e d m o r e f r e q u e n t l y in c h r i s t i a n s t h a n in m o s l e m s . 3- A 9 , B 5 , B 3 5 , B 2 1 and Cw4 o c c u r r e d m o r e f r e q u e n t l y in leba n e s e ( c h r i s t i a n and m o s l e m ) t h a n in e u r o p e a n s . 4- B27 and Cw3 o c c u r r e d m o r e f r e q u e n t l y in e u r o p e a n s t h a n in l e b a n e s e . 5- T h e r e w e r e o t h e r m i n o r d i f f e r e n c e s in HLA f r e q u e n c i e s a m o n g the g r o u p s c o m p a r e d . In c o n c l u s i o n , t h e s e r e s u l t s may be u s e f u l in d i s e a s e association and a n t h r o p o l o g i c a l s t u d i e s in l e b a n e s e .
109
B 9.1.205
CELL-FREE ASSAY PREDICTS PROLIFERATIVE SUPPRESSION BY CYCLOSPORINE AND RAPAMYCIN. PM Kimball, RH Kerman, and BD Kahan, Univeristy of Texas Medical School, Houston, Texas. We report that Cyclosporine (CsA) and Rapamycin (RAPA) inhibit protein kinase C[PKC]-triggering of the intracellular activation signal ADR (Activator of DNA Replication) in a cell-free assay. Furthermore, ADR induction is dosedependent and correlates with proliferative suppression by CsA or RAPA. Peripheral blood lymphocyte cytosol was isolated and endogenous PKC triggered with 50 nM phorbol myristate acetate (PMA). ADR was subsequently measured by uptake of tritiated thymidine. PKC activation induced ADR (890+ 120 cpm v~ 3910+345, p<0.001). In contrast, PKC inhibition by H-7 blocked ADR generation (100+50 cpm vs 3910+345, p<0.001). ADR was not found following cell cultivation with PHA and 50 nM CsA (910+40 cpm with CsA vs 6410+830 cpm without CsA, p<0.001) nor was it induced by PKC (750+80 cpm). Pretreatment of unstimulated cells with CsA or RAPA blocked ADR induction by PKC. CsA doses of 10, 50 or 100 ng/ml inhibited ADR induction 10%, 59% or 73% and T-cell mitogenesis by 15%, 63% or 79%, respectively. RAPA pretreatment of resting cells resulted in similar correlations between ADR inducability versus proliferation suppression. In summary, cytosolic ADR was generated following PKC activation. Cell-free quantitation of ADR mimiced proliferative inhibition by CsA and RAPA. Thus, cell-free assay of ADR may permit monitoring of clinical immunosuppression as well as dissection of phosphorylated intermediates necessary for intracellular communication.
B 9.1.206
H L A - A , B AND C F R E Q U E N C I E S IN L E B A N E S E . M a y A b d e l n o o r and A l e x a n d e r M. A b d e l n o o r , N o t r e D a m e D e s S e c o u r s H o s p i t a l an~--~merlcan ~n-i-~ersity of B e i r u t , L e b a n o n . H L A - A , B and C f r e q u e n c i e s w e r e d e t e r m i n e d in 416 lebanese.These i n c l u d e d 237 m o s l e m s and 179 c h r i s t i a n s . A m o n g the m o s l e m s t h e r e w e r e 149 c h i i t e s and 88 s u n n i t e s . A m o n g the c h r i s t i a n s t h e r e w e r e 129 m a r o n i t e s , 2 7 o r t h o d o x and 23 c a t h o l i c s . The m e t h o d u s e d for HLA d e t e r m i n a t i o n s w a s the c o m p l e m e n t d e p e n d e n t m i c r o c y t o t o x i c i t y assay. The f r e q u e n c i e s of the v a r i o u s H L A - A , B and C d e t e r m i n e d in leba n e s e c h r i s t i a n s and m o s l e m s w e r e c o m p a r e d to e a c h o t h e r and to t h o s e of r e p o r t e d f r e q u e n c i e s for e u r o p e a n s . T h e r e s u l t s i n d i c a t e d that; i- A 2 , B 7 and B27 o c c u r r e d m o r e f r e q u e n t l y in m o s l e m s t h a n in c h r i s t i a n s . 2- A 9 , B 3 5 and Cw4 o c c u r r e d m o r e f r e q u e n t l y in c h r i s t i a n s t h a n in m o s l e m s . 3- A 9 , B 5 , B 3 5 , B 2 1 and Cw4 o c c u r r e d m o r e f r e q u e n t l y in leba n e s e ( c h r i s t i a n and m o s l e m ) t h a n in e u r o p e a n s . 4- B27 and Cw3 o c c u r r e d m o r e f r e q u e n t l y in e u r o p e a n s t h a n in l e b a n e s e . 5- T h e r e w e r e o t h e r m i n o r d i f f e r e n c e s in HLA f r e q u e n c i e s a m o n g the g r o u p s c o m p a r e d . In c o n c l u s i o n , t h e s e r e s u l t s may be u s e f u l in d i s e a s e association and a n t h r o p o l o g i c a l s t u d i e s in l e b a n e s e .