ANALYTICALBIOCHEMISTRY
148, 546(1985)
BOOK
REVIEW
Practice of Chromatography. By COLIN F. POOLE AND SHIELA A. SCHUETTE, Elsevier, Amsterdam/New York, 1984. 708 pp. $61.25.
Contemporary
The traditional approaches to separations scienceand practice-are complemented in this book by an emphasis on chromatographic technology. A compact theoretical introduction is followed by a detailed discussion of GC column technology, including details of making glass capillary columns, with CC instrumentation discussed next. A similar pairing of chapters is used to present HPLC, treating first the column, solvent, column packing, and gradient, and then HPLC instrumentation. Scale-up is also treated succinctly for both CiC and LC. The chapter on sample preparation includes a survey of approaches that emphasize liquid-liquid partition, sorptive preconcentration, and headspace analysis. Preand postcolumn derivatization topics include detection enhancement with CC and LC, and TLC visualization as well. The review presented in this chapter cites 658 references. The closing chapters offer a summary of “hyphenated” systems (i.e., chromatograph-detector) including MS, FTIR, NMR, and optical emission. The last chapter deals with high-performance TLC. principles
Briefly Proteins and Peptides, Vol. 12, Growth FacEdited by C. H. LI, Academic Press, Orlando/ New York, 1984. 307 pp, $65.00.
Hormonal tars.
Biological and chemical properties of several growth factors are presented. These include the nerve growth factor (Cal&no, Cattaneo, Aloe, and Levi-Montalcini); the group of insulin-like growth factors represented by three articles (Ham&l, Van Wyle, and Nissley and Rechler); the fibroblast growth factors from brain and pituitary (Gospodarowicz); human platelet-derived growth factor (Antoniades and Owen); and skeletal growth factor (Linkhart, Mohan, Jennings, Farley, and Baylink). A
0003-2697185 $3.00 Copyright 0 1985 by Academic Press. Inc. All rights of reproduction in any form wewed.
The book provides over 2000 literature references, mostly recent, but not all subjects are treated evenly. Solid-phase extraction is an example of one topic that could have been included but was not. The practicum is slanted more toward environmental rather than biological applications. This book organizes much technical detail. It is recommended for addition to the library of the analytical worker. With its many tables, diagrams, illustrated equations, and calculations, it is both a resource and reference text and to a lesser extent, a teaching text. The subject index is too abbreviated and it does not list examples by sample or compound type. This reflects dealing primarily with chromatographic techniques by omitting unneeded theory, and explaining chromatographic practice using the minimum number of actual chromatograms to get the point across. There are many articles and texts already available that deal primarily with analysis by compound, by sample type, or by field. This book creates its own niche by compiling in carefully organized form many practical features of the instrumented chemical separations technologies that are known to have practical utility. DAVID H. FREEMAN
Noted final chapter of five pages on epidermal growth factor (Cohen) is limited to a “historical perspective.” Edited by P. (A. Meister, series ed.) Wiley, New York, 1985. 643 pp. $125.00.
Biochemistry, CHRISTEN
Vol. 2, Transaminases.
AND D.
E. METZLER
Although concentrating on transaminases (aminotransferases), this is an extensive examination of pyridoxal phosphate and of enzymes that utilize this coenzyme. Included is material on enzyme structure, reaction mechanism, and metabolic aspects, with a final touch of clinical pathology.
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