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HPLC of macromolecules a practical approach
HPLC of Macromolecules a Practical Approach
Edited by R. W. A. Oliver Oxford University Press, Oxford, 1989, pp. 252, £27 spiral bound, £I8 paperback Although not obvious from the title, the book is completely orientated to the h.p.l.c, of biological macromolecules and, in practice, the bulk of the book mainly relates to handling proteins and peptides. The emphasis of the book is clearly on a practical approach. It is ideally suited to those with no experience of h.p.l.c, and while experienced practitioners may well benefit from the detailed advice contained within the text, much of which is conveniently tabulated, they would be likely to find the general approach rather pedantic. If read from start to finish there is a degree of repetition, but this has the advantage that individual chapters consulted are reasonably self-contained. The first chapter of the book gives a very comprehensive review of the large
0141-8130/89/050317~ 1$03.00 © 1989 Butterworth & Co. (Publishers) Ltd
variety of h.p.i.c, column packings available for all forms of chromatographic separations related to biological macromolecules and the second chapter gives a thorough discussion of the instrumental requirements of such systems. These first two chapters are the only general chapters. Chapters 3 to 6 give detailed consideration, respectively, of sizeexclusion, ion-exchange, reversed-phase and alfinity chromatography of proteins and peptides. As stated above, these chapters are quite self-contained, they give very detailed advice on setting up chromatographic systems and a good part of each chapter is devoted to sample applications. Chapter 7 covers some of the same chromatographic techniques, but as
applied to the h.p.l.c, of oligonucleotides. This chapter keeps to the same approach of experimental detail and examples of applications. A similar approach is also taken in the final chapter which covers the use of lectins for the affinity chromatography of carbohydrates. The h.p.l.c, of biological macromolecules has advanced considerably in recent years and this book has probably been produced at quite an appropriate stage of development. The text will be found valuable by those approaching the general subject for the first time or for those with limited experience of h.p.l.c.; others may also find useful information in the extensive practical details and the large number of cited applications. Steve Holding
Int. J. Biol. Macromol., 1989, Vol. 11, O c t o b e r
317
Edited by R. W. A. Oliver Oxford University Press, Oxford, 1989, pp. 252, £27 spiral bound, £I8 paperback Although not obvious from the title, the book is completely orientated to the h.p.l.c, of biological macromolecules and, in practice, the bulk of the book mainly relates to handling proteins and peptides. The emphasis of the book is clearly on a practical approach. It is ideally suited to those with no experience of h.p.l.c, and while experienced practitioners may well benefit from the detailed advice contained within the text, much of which is conveniently tabulated, they would be likely to find the general approach rather pedantic. If read from start to finish there is a degree of repetition, but this has the advantage that individual chapters consulted are reasonably self-contained. The first chapter of the book gives a very comprehensive review of the large
0141-8130/89/050317~ 1$03.00 © 1989 Butterworth & Co. (Publishers) Ltd
variety of h.p.i.c, column packings available for all forms of chromatographic separations related to biological macromolecules and the second chapter gives a thorough discussion of the instrumental requirements of such systems. These first two chapters are the only general chapters. Chapters 3 to 6 give detailed consideration, respectively, of sizeexclusion, ion-exchange, reversed-phase and alfinity chromatography of proteins and peptides. As stated above, these chapters are quite self-contained, they give very detailed advice on setting up chromatographic systems and a good part of each chapter is devoted to sample applications. Chapter 7 covers some of the same chromatographic techniques, but as
applied to the h.p.l.c, of oligonucleotides. This chapter keeps to the same approach of experimental detail and examples of applications. A similar approach is also taken in the final chapter which covers the use of lectins for the affinity chromatography of carbohydrates. The h.p.l.c, of biological macromolecules has advanced considerably in recent years and this book has probably been produced at quite an appropriate stage of development. The text will be found valuable by those approaching the general subject for the first time or for those with limited experience of h.p.l.c.; others may also find useful information in the extensive practical details and the large number of cited applications. Steve Holding
Int. J. Biol. Macromol., 1989, Vol. 11, O c t o b e r
317