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Human growth factor and cytokine skin cream promotes neocollagenesis in vivo
P923
P925
High-petrolatum depositing body wash cleans efficiently and improves dry skin condition Keith Ertel, PhD, MS, The Procter & Gamble Company, Cincinnati, OH, United States; Heather Focht, The Procter & Gamble Company, Cincinnati, OH, United States; Robert Bacon, The Procter & Gamble Company, Cincinnati, OH, United States; Laura Lebda, MS, The Procter & Gamble Company, Cincinnati, OH, United States Background: The skin is covered with a natural hydrolipid film that serves functions such as helping to maintain pliability and providing a natural defense against pathogenic organisms. But this film also attracts and holds dirt and pollutants from the environment and microbial action on components of the film can lead to odor. Thus, while the surface hydrolipid film is an important skin integument, periodic cleansing to remove dirt, debris, and odor is essential to enhancing skin health and in many cultures, for social acceptance. Additionally, periodic cleansing is necessary to remove soil from the skin surface that is acquired by incidental contact or by intentional application such as topical medicaments or makeup and other cosmetic products. Traditional thinking holds that a personal cleansing product must negatively affect skin to clean effectively, an apparent dilemma for individuals with dermatologic conditions.
Human growth factor and cytokine skin cream promotes neocollagenesis in vivo Robert Phelps, MD, Mt. Sinai School of Medicine, New York, NY, United States; Mussarrt Hussain, MD, Skin Laser and Surgery Specialists of New York and New Jersey, Westwood, NJ, United States; David J. Goldberg, MD, Skin Laser and Surgery Specialists of New York and New Jersey, New York, NY, United States
Objective: This study was conducted to compare the cleansing efficiency of a highpetrolatum depositing body wash (HPDBW) and a dermatologist-recognized synthetic detergent (syndet) bar. Method: An oil-based makeup material was chosen as a model soil, because many difficult to remove soils are lipophilic in nature. Makeup has the added advantage of being commercially available and colored, which aids in detection. Thirteen healthy, light-skinned females were recruited. The model soil was applied to discrete spots on the volar forearms then the spots were washed in a controlled manner with lather from the randomly assigned cleanser. One site was washed with water only as a control. Cleansing efficiency was calculated as the difference between chromameter measurements made on each site pre- and postwashing. The cleansers’ potential to moisturize skin was assessed relative to a water control in studies conducted using a published leg wash procedure. Results: The bar removed the model soil better than the control (P \.01), and the HDPBW removed the model soil better than the bar (P \.01). The leg wash tests show that after 5 days the HDPBW improved dryness, stratum corneum hydration, and barrier function compared to control (P \.01, n ¼ 41), while the bar did not (P[.15, n ¼ 39). Taken together, these results show that the HPDBW offers patients a personal cleansing option that cleans efficiently and can also improve dry skin condition.
The crucial role of growth factors and cytokines in cutaneous wound healing has been well described. However, their use for skin rejuvenation is only now emerging. In principle, growth factors may participate in skin rejuvenation at various levels. With topical application, they may promote the growth of keratinocytes, dermal fibroblasts, and other cells with decreased prolific capacity because of aging. This is similar to the effects observed in cutaneous wound healing. Further, some growth factors and cytokines are potent stimulators of the expression of extracellular matrix proteins, including collagen. A reduced capacity to synthesize collagen relative to healthy young skin is characteristic for chronologically and photoaged skin. The present study aimed to assess new collagen formation after topical application of a skin cream containing a proprietary mixture of human growth factors and cytokines by electron microscopy. In previous studies, the cream was shown to significantly rejuvenate the signs of aged facial skin. The mixture was obtained through a biotechnology process using cultured human fetal skin cells, which originate from a dedicated cell bank originally established for the development of products in wound healing. After obtaining informed consent, 12 female volunteers between 35 and 65 years of age were asked to apply the cream twice daily on their entire face over a period of 6 months. A 3-mm punch biopsy was taken from the preauricular skin area before and after the treatment. Collagen fiber diameter was measured as first described by Fleischmajer et al in 1981. Collagen diameter data was available for 7 subjects and revealed a global increase in smaller diameter collagen fibers with a shift towards fibers smaller than 60 nm in the superficial dermis after the 6-month treatment period with the cream. Four out of seven subjects showed a significantly reduced averaged collagen diameter by 8% or more, whereas in two subjects collagen diameter did not change and slightly increased in one. This observation is consistent with primarily new collagen type III formation, which is smaller diameter than collagen I. These results confirm earlier studies demonstrating stimulation of collagen formation also under in use conditions for the growth factor and cytokine skin cream and represents further evidence for the beneficial use of such products to rejuvenate aged skin. 100% funded by Neocutis, Inc., San Francisco, CA.
100% sponsored by The Procter & Gamble Company.
P924 Facial foundation to provide skin condition benefits Keith Ertel, PhD, MS, The Procter & Gamble Company, Cincinnati, OH, United States; Zoe Diana Draelos, MD, PA, Dermatology Consulting Services, High Point, NC, United States; Rosemarie Osborne, PhD, The Procter & Gamble Company, Cincinnati, OH, United States; Gary Kelm, PhD, The Procter & Gamble Company, Cincinnati, OH, United States Background: Skin appearance, and especially facial skin appearance, is an important contributor to social acceptance and an individual’s sense of well-being. Dermatologic procedures that reduce the appearance of facial aging are of interest to many patients, and cosmetics can augment the positive appearance benefits these procedures provide. Because facial aging involves skin damage, improving skin condition is also desirable. We developed a facial foundation product that contains niacinamide (NCA) and N-acetyl glucosamine (NAG), cosmetic ingredients that the literature reports can positively impact skin condition. Objective: To assess whether the facial foundation product with NCA and NAG can provide skin benefits beyond simple skin appearance improvement. Method: In vitro studies were conducted using skin-equivalent cultures or cadaver skin to study the effect of NCA 1 NAG application on hyaluronic acid expression, and to study NCA and NAG skin penetration from the facial foundation product, respectively. A randomized 5-day facial study was conducted in which the facial foundation product was applied once daily below one eye; the area below the other eye remained untreated as a control. Skin elasticity below each eye was measured at baseline and 4 hours after the final treatment. A randomized single-application study was conducted to assess the skin hydrating properties of the facial foundation relative to an untreated control. Skin capacitance, an indicator of stratum corneum hydration, was measured at baseline and 4 and 8 hours after application.
P926 A randomized, double-blind, vehicle-controlled study of the safety and efficacy of a nutrient solution containing multiple growth factors and cytokines in mild to severe facial photodamage Stacy Smith, MD, Therapeutics Clinical Research, San Diego, CA, United States Background: Studies of the topical application of growth factors and cytokines to intact photodamaged skin are becoming more common as objective measurement techniques have been developed. In this study, a nutrient solution gel containing a combination of multiple growth factors, cytokines, antioxidants, and matrix proteins secreted by human dermal fibroblasts was evaluated for safety and efficacy in the treatment of mild to severe facial photodamage. The study was conducted at a single center as a randomized, double-blind, vehicle-controlled design. Previous pilot studies have shown positive restorative effects with this nutrient solution gel. Methods: Subjects were randomly assigned to receive either nutrient solution gel or vehicle and apply it twice daily for 6 months. At each visit (months 0, 3, and 6), efficacy measures (evaluator-blinded assessments of photodamage, silicon replica evaluations, and standardized photography) and safety measures (adverse event reporting) were performed.
Results: Application of NCA 1 NAG significantly (P ¼.02) increased the expression of hyaluronic acid compared to control within 24 hours in skin-equivalent cultures. Hyaluronic acid is linked with skin moisturization and anti-aging benefits. The skin penetration study conducted with the facial foundation product showed that significant amounts (P \.10) of NCA and NAG permeate the stratum corneum from the formulated product within 6 hours. The in vivo tests showed that the facial foundation significantly improved below-eye skin elasticity (P \.01) and increased stratum corneum hydration (P \.01) compared to control. These results show that the facial foundation with niacinamide and N-acetyl glucosamine is a good choice for patients desiring a cosmetic product to help improve their skin’s appearance and condition.
Results: Sixty subjects (6 male and 54 female) aged 25 to 65 years with mild to severe facial photodamage were enrolled, and 58 completed the study. After 6 months of nutrient solution gel treatment, statistically significant clinical improvements were observed in fine wrinkling overall, mottled pigmentation, and tactile roughness compared with pretreatment (P # .05). These subjective clinical findings were corroborated by the results of the silicon replica evaluations (optical profilometry) which demonstrated a statistically significant reduction (P #.03) in wrinkles at both 3 and 6 months posttreatment. Optical profilometry analyses revealed significant benefits favoring the active group with regard to decreasing wrinkle depth, improving skin texture, and increasing skin smoothness. Further, a blinded review of the photographs by three independent expert dermatologists found statistically significant differences between nutrient solution gel and vehicle treated subjects in the severely photodamaged subgroup. Nutrient solution gel was safe and well tolerated; no subjects withdrew from the study because of adverse experiences. This study demonstrated very favorable results in subjective clinical assessments, optical profilometry, and independent photographic review.
100% sponsored by The Procter & Gamble Company.
100% is sponsored by SkinMedica, Inc.
AB68
J AM ACAD DERMATOL
FEBRUARY 2008
P925
High-petrolatum depositing body wash cleans efficiently and improves dry skin condition Keith Ertel, PhD, MS, The Procter & Gamble Company, Cincinnati, OH, United States; Heather Focht, The Procter & Gamble Company, Cincinnati, OH, United States; Robert Bacon, The Procter & Gamble Company, Cincinnati, OH, United States; Laura Lebda, MS, The Procter & Gamble Company, Cincinnati, OH, United States Background: The skin is covered with a natural hydrolipid film that serves functions such as helping to maintain pliability and providing a natural defense against pathogenic organisms. But this film also attracts and holds dirt and pollutants from the environment and microbial action on components of the film can lead to odor. Thus, while the surface hydrolipid film is an important skin integument, periodic cleansing to remove dirt, debris, and odor is essential to enhancing skin health and in many cultures, for social acceptance. Additionally, periodic cleansing is necessary to remove soil from the skin surface that is acquired by incidental contact or by intentional application such as topical medicaments or makeup and other cosmetic products. Traditional thinking holds that a personal cleansing product must negatively affect skin to clean effectively, an apparent dilemma for individuals with dermatologic conditions.
Human growth factor and cytokine skin cream promotes neocollagenesis in vivo Robert Phelps, MD, Mt. Sinai School of Medicine, New York, NY, United States; Mussarrt Hussain, MD, Skin Laser and Surgery Specialists of New York and New Jersey, Westwood, NJ, United States; David J. Goldberg, MD, Skin Laser and Surgery Specialists of New York and New Jersey, New York, NY, United States
Objective: This study was conducted to compare the cleansing efficiency of a highpetrolatum depositing body wash (HPDBW) and a dermatologist-recognized synthetic detergent (syndet) bar. Method: An oil-based makeup material was chosen as a model soil, because many difficult to remove soils are lipophilic in nature. Makeup has the added advantage of being commercially available and colored, which aids in detection. Thirteen healthy, light-skinned females were recruited. The model soil was applied to discrete spots on the volar forearms then the spots were washed in a controlled manner with lather from the randomly assigned cleanser. One site was washed with water only as a control. Cleansing efficiency was calculated as the difference between chromameter measurements made on each site pre- and postwashing. The cleansers’ potential to moisturize skin was assessed relative to a water control in studies conducted using a published leg wash procedure. Results: The bar removed the model soil better than the control (P \.01), and the HDPBW removed the model soil better than the bar (P \.01). The leg wash tests show that after 5 days the HDPBW improved dryness, stratum corneum hydration, and barrier function compared to control (P \.01, n ¼ 41), while the bar did not (P[.15, n ¼ 39). Taken together, these results show that the HPDBW offers patients a personal cleansing option that cleans efficiently and can also improve dry skin condition.
The crucial role of growth factors and cytokines in cutaneous wound healing has been well described. However, their use for skin rejuvenation is only now emerging. In principle, growth factors may participate in skin rejuvenation at various levels. With topical application, they may promote the growth of keratinocytes, dermal fibroblasts, and other cells with decreased prolific capacity because of aging. This is similar to the effects observed in cutaneous wound healing. Further, some growth factors and cytokines are potent stimulators of the expression of extracellular matrix proteins, including collagen. A reduced capacity to synthesize collagen relative to healthy young skin is characteristic for chronologically and photoaged skin. The present study aimed to assess new collagen formation after topical application of a skin cream containing a proprietary mixture of human growth factors and cytokines by electron microscopy. In previous studies, the cream was shown to significantly rejuvenate the signs of aged facial skin. The mixture was obtained through a biotechnology process using cultured human fetal skin cells, which originate from a dedicated cell bank originally established for the development of products in wound healing. After obtaining informed consent, 12 female volunteers between 35 and 65 years of age were asked to apply the cream twice daily on their entire face over a period of 6 months. A 3-mm punch biopsy was taken from the preauricular skin area before and after the treatment. Collagen fiber diameter was measured as first described by Fleischmajer et al in 1981. Collagen diameter data was available for 7 subjects and revealed a global increase in smaller diameter collagen fibers with a shift towards fibers smaller than 60 nm in the superficial dermis after the 6-month treatment period with the cream. Four out of seven subjects showed a significantly reduced averaged collagen diameter by 8% or more, whereas in two subjects collagen diameter did not change and slightly increased in one. This observation is consistent with primarily new collagen type III formation, which is smaller diameter than collagen I. These results confirm earlier studies demonstrating stimulation of collagen formation also under in use conditions for the growth factor and cytokine skin cream and represents further evidence for the beneficial use of such products to rejuvenate aged skin. 100% funded by Neocutis, Inc., San Francisco, CA.
100% sponsored by The Procter & Gamble Company.
P924 Facial foundation to provide skin condition benefits Keith Ertel, PhD, MS, The Procter & Gamble Company, Cincinnati, OH, United States; Zoe Diana Draelos, MD, PA, Dermatology Consulting Services, High Point, NC, United States; Rosemarie Osborne, PhD, The Procter & Gamble Company, Cincinnati, OH, United States; Gary Kelm, PhD, The Procter & Gamble Company, Cincinnati, OH, United States Background: Skin appearance, and especially facial skin appearance, is an important contributor to social acceptance and an individual’s sense of well-being. Dermatologic procedures that reduce the appearance of facial aging are of interest to many patients, and cosmetics can augment the positive appearance benefits these procedures provide. Because facial aging involves skin damage, improving skin condition is also desirable. We developed a facial foundation product that contains niacinamide (NCA) and N-acetyl glucosamine (NAG), cosmetic ingredients that the literature reports can positively impact skin condition. Objective: To assess whether the facial foundation product with NCA and NAG can provide skin benefits beyond simple skin appearance improvement. Method: In vitro studies were conducted using skin-equivalent cultures or cadaver skin to study the effect of NCA 1 NAG application on hyaluronic acid expression, and to study NCA and NAG skin penetration from the facial foundation product, respectively. A randomized 5-day facial study was conducted in which the facial foundation product was applied once daily below one eye; the area below the other eye remained untreated as a control. Skin elasticity below each eye was measured at baseline and 4 hours after the final treatment. A randomized single-application study was conducted to assess the skin hydrating properties of the facial foundation relative to an untreated control. Skin capacitance, an indicator of stratum corneum hydration, was measured at baseline and 4 and 8 hours after application.
P926 A randomized, double-blind, vehicle-controlled study of the safety and efficacy of a nutrient solution containing multiple growth factors and cytokines in mild to severe facial photodamage Stacy Smith, MD, Therapeutics Clinical Research, San Diego, CA, United States Background: Studies of the topical application of growth factors and cytokines to intact photodamaged skin are becoming more common as objective measurement techniques have been developed. In this study, a nutrient solution gel containing a combination of multiple growth factors, cytokines, antioxidants, and matrix proteins secreted by human dermal fibroblasts was evaluated for safety and efficacy in the treatment of mild to severe facial photodamage. The study was conducted at a single center as a randomized, double-blind, vehicle-controlled design. Previous pilot studies have shown positive restorative effects with this nutrient solution gel. Methods: Subjects were randomly assigned to receive either nutrient solution gel or vehicle and apply it twice daily for 6 months. At each visit (months 0, 3, and 6), efficacy measures (evaluator-blinded assessments of photodamage, silicon replica evaluations, and standardized photography) and safety measures (adverse event reporting) were performed.
Results: Application of NCA 1 NAG significantly (P ¼.02) increased the expression of hyaluronic acid compared to control within 24 hours in skin-equivalent cultures. Hyaluronic acid is linked with skin moisturization and anti-aging benefits. The skin penetration study conducted with the facial foundation product showed that significant amounts (P \.10) of NCA and NAG permeate the stratum corneum from the formulated product within 6 hours. The in vivo tests showed that the facial foundation significantly improved below-eye skin elasticity (P \.01) and increased stratum corneum hydration (P \.01) compared to control. These results show that the facial foundation with niacinamide and N-acetyl glucosamine is a good choice for patients desiring a cosmetic product to help improve their skin’s appearance and condition.
Results: Sixty subjects (6 male and 54 female) aged 25 to 65 years with mild to severe facial photodamage were enrolled, and 58 completed the study. After 6 months of nutrient solution gel treatment, statistically significant clinical improvements were observed in fine wrinkling overall, mottled pigmentation, and tactile roughness compared with pretreatment (P # .05). These subjective clinical findings were corroborated by the results of the silicon replica evaluations (optical profilometry) which demonstrated a statistically significant reduction (P #.03) in wrinkles at both 3 and 6 months posttreatment. Optical profilometry analyses revealed significant benefits favoring the active group with regard to decreasing wrinkle depth, improving skin texture, and increasing skin smoothness. Further, a blinded review of the photographs by three independent expert dermatologists found statistically significant differences between nutrient solution gel and vehicle treated subjects in the severely photodamaged subgroup. Nutrient solution gel was safe and well tolerated; no subjects withdrew from the study because of adverse experiences. This study demonstrated very favorable results in subjective clinical assessments, optical profilometry, and independent photographic review.
100% sponsored by The Procter & Gamble Company.
100% is sponsored by SkinMedica, Inc.
AB68
J AM ACAD DERMATOL
FEBRUARY 2008