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Hyperglycemic effect of leaves of Mimosa pudica Linn
Fitoterapia 73 Ž2002. 351᎐352
Short report
Hyperglycemic effect of leaves of Mimosa pudica Linn T. Amalraj, S. IgnacimuthuU,1 Entomology Research Institute, Loyola College, Chennai-600 034, India Received 26 May 2001; accepted in revised form 5 April 2002
Abstract Ethanolic extract of Mimosa pudica leaves given by oral route to mice at a dose of 250 mgrkg showed a significant hyperglycemic effect. 䊚 2002 Elsevier Science B.V. All rights reserved. Keywords: Mimosa pudica; Hyperglycemic activity
Plant. Mimosa pudica Linn. ŽMimosaceae. leaves collected from Chengalpattu District, Tamilnadu, India in August 2000 were authenticated by the taxonomist of the Entomology Research Institute. A voucher specimen is deposited in our Institute. Use in traditional medicine. Leaves and roots in the powdered form are given to treat piles and fistula. Roots in the form of decoction are used to treat urinary complaints and in diseases arising from corrupt blood and bile. The paste of the leaves is applied to glandular swelling and dressing for sinus w1x. Previously isolated compounds. Tannins, mimosine, steroids w1x. Animal. Swiss albino mice of either sex Ž30᎐35 g. were housed in standard laboratory conditions and fed standard balanced diet pellet and water ad libitum. U
Corresponding author. Tel. q91-44-826-5542; fax: q91-44-826-5544. E-mail address: eri ᎏ [email protected] ŽS. Ignacimuthu.. 1 Present address: Vice Chancellor, Bharathiar University, Coimbatore, India.
0367-326Xr02r$ - see front matter 䊚 2002 Elsevier Science B.V. All rights reserved. PII: S 0 3 6 7 - 3 2 6 X Ž 0 2 . 0 0 0 7 9 - 5
352
T. Amalraj, S. Ignacimuthu r Fitoterapia 73 (2002) 351᎐352
Tested material. Air-dried and powdered leaf sample Ž100 g. was extracted in 80% ethanol, filtered and concentrated in a rotary evaporator. The dried extract Ž6% yield. was used for the present studies. Phytochemical screening w2x gave positive tests for phenols, steroids and carbohydrates. Studied activity. Hyperglycemic activity in normal and glucose loaded mice by measuring the serum glucose levels w3x. Results. Reported in Table 1. Table 1 Hyperglycemic activity of ethanolic extract of Mimosa pudica leaves Treatment p.o.
0 min
Plasma glucose levels Žmgrdl. 60 min
90 min
120 min
1. Control 2. M. pudica 250 mgrkg 3. Glucose control Ž1 g. 4. Glucose q M. pudica
94.67 " 3.56 101.91 " 6.78 95.55 " 5.75 104.33 " 7.44
101.73 " 5.2 149.84 " 9.6a 127.33 " 10.2 195.64 " 12.7b
106.07 " 4.7 168.22 " 9.2a 142.54 " 8.4 232.63 " 16.2b
102.67 " 5.4 155.23 " 10.1a 122.89 " 9.6 186.77 " 14.2b
Values are mean " S.E., n s 6. a Statistically significant pc 0.001 ŽStudent’s t-test. vs. Group 1. b Statistically significant pc 0.001 ŽStudent’s t-test. vs. Group 3.
Conclusion. Based on the results of this study, we conclude that the ethanolic extract of M. pudica leaves, given orally at a dose of 250 mgrkg, possesses significant hyperglycemic effects both in normal and glucose loaded mice. As a consequence, the use of M. pudica leaves orally prescribed to treat various ailments in the traditional medicine deserves careful evaluation.
Acknowledgements We are grateful to the Mac Arthur Foundation, Chicago, USA for the financial support.
References w1x Sastri BN. The wealth of India, vol. 6. New Delhi, India: CSIR, 1962:382. w2x Paech D, Tracey MV. Modern methods of plant analysis, 1955, p. 373. w3x Sasaki T, Matusi S. Rinsho Kagoku, 1972:346.
Short report
Hyperglycemic effect of leaves of Mimosa pudica Linn T. Amalraj, S. IgnacimuthuU,1 Entomology Research Institute, Loyola College, Chennai-600 034, India Received 26 May 2001; accepted in revised form 5 April 2002
Abstract Ethanolic extract of Mimosa pudica leaves given by oral route to mice at a dose of 250 mgrkg showed a significant hyperglycemic effect. 䊚 2002 Elsevier Science B.V. All rights reserved. Keywords: Mimosa pudica; Hyperglycemic activity
Plant. Mimosa pudica Linn. ŽMimosaceae. leaves collected from Chengalpattu District, Tamilnadu, India in August 2000 were authenticated by the taxonomist of the Entomology Research Institute. A voucher specimen is deposited in our Institute. Use in traditional medicine. Leaves and roots in the powdered form are given to treat piles and fistula. Roots in the form of decoction are used to treat urinary complaints and in diseases arising from corrupt blood and bile. The paste of the leaves is applied to glandular swelling and dressing for sinus w1x. Previously isolated compounds. Tannins, mimosine, steroids w1x. Animal. Swiss albino mice of either sex Ž30᎐35 g. were housed in standard laboratory conditions and fed standard balanced diet pellet and water ad libitum. U
Corresponding author. Tel. q91-44-826-5542; fax: q91-44-826-5544. E-mail address: eri ᎏ [email protected] ŽS. Ignacimuthu.. 1 Present address: Vice Chancellor, Bharathiar University, Coimbatore, India.
0367-326Xr02r$ - see front matter 䊚 2002 Elsevier Science B.V. All rights reserved. PII: S 0 3 6 7 - 3 2 6 X Ž 0 2 . 0 0 0 7 9 - 5
352
T. Amalraj, S. Ignacimuthu r Fitoterapia 73 (2002) 351᎐352
Tested material. Air-dried and powdered leaf sample Ž100 g. was extracted in 80% ethanol, filtered and concentrated in a rotary evaporator. The dried extract Ž6% yield. was used for the present studies. Phytochemical screening w2x gave positive tests for phenols, steroids and carbohydrates. Studied activity. Hyperglycemic activity in normal and glucose loaded mice by measuring the serum glucose levels w3x. Results. Reported in Table 1. Table 1 Hyperglycemic activity of ethanolic extract of Mimosa pudica leaves Treatment p.o.
0 min
Plasma glucose levels Žmgrdl. 60 min
90 min
120 min
1. Control 2. M. pudica 250 mgrkg 3. Glucose control Ž1 g. 4. Glucose q M. pudica
94.67 " 3.56 101.91 " 6.78 95.55 " 5.75 104.33 " 7.44
101.73 " 5.2 149.84 " 9.6a 127.33 " 10.2 195.64 " 12.7b
106.07 " 4.7 168.22 " 9.2a 142.54 " 8.4 232.63 " 16.2b
102.67 " 5.4 155.23 " 10.1a 122.89 " 9.6 186.77 " 14.2b
Values are mean " S.E., n s 6. a Statistically significant pc 0.001 ŽStudent’s t-test. vs. Group 1. b Statistically significant pc 0.001 ŽStudent’s t-test. vs. Group 3.
Conclusion. Based on the results of this study, we conclude that the ethanolic extract of M. pudica leaves, given orally at a dose of 250 mgrkg, possesses significant hyperglycemic effects both in normal and glucose loaded mice. As a consequence, the use of M. pudica leaves orally prescribed to treat various ailments in the traditional medicine deserves careful evaluation.
Acknowledgements We are grateful to the Mac Arthur Foundation, Chicago, USA for the financial support.
References w1x Sastri BN. The wealth of India, vol. 6. New Delhi, India: CSIR, 1962:382. w2x Paech D, Tracey MV. Modern methods of plant analysis, 1955, p. 373. w3x Sasaki T, Matusi S. Rinsho Kagoku, 1972:346.