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Hypericinates preparation and comparative study of cell viability with hypericin
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Abstracts / Photodiagnosis and Photodynamic Therapy 12 (2015) 325–375
Multifunctional porphyrin-based polysilsesquioxane nanoparticles with improved loading capacity and phototherapeutic effect Juan L. Vivero-Escoto 1,2 , Zachary Lyles 1,2 , Daniel L. Vega 1,2
the peptides to normal colon cell line. We confirmed that peptide conjugated photosensitizer had enhanced photodynamic therapy (PDT) effect compared to none conjugated photosensitizer. The results from this study suggest that targeted PDT using peptide probe may be a promising candidate drug in the development of a useful colon cancer diagnosis and treatment. http://dx.doi.org/10.1016/j.pdpdt.2015.07.063
1
Department of Chemistry, University of North Carolina at Charlotte, Charlotte, NC 28223, USA 2 The Center for Biomedical Engineering and Science, University of North Carolina at Charlotte, Charlotte, NC 28223, USA The application of nanoparticles as delivery platforms in PDT is a promising alternative for resolving some of the major challenges associated with classic photosensitizers (PSs) such as porphyrin molecules. Polysilsesquioxane nanoparticles (PSQ-NPs) are crosslinked homopolymers formed by the condensation of functionalized trialkoxysilanes. Recent reports have shown that PSQ-NPs can provide an interesting platform for developing PS nanocarriers. Several advantages can be foreseen by using this platform such as carrying a large payload of PS molecules; their surface and composition can be tailored to develop multifunctional systems. In this work, two PSQ-NP systems with a high payload of photosensitizers were synthesized, characterized, and applied in vitro. The network of these nanomaterials is formed by two different types of porphyrin-based photosensitizers, protoporphyrin-IX and tetrakis(4-carboxyphenyl)porphyrin, chemically connected via a redox-responsive linker. Both platforms can be further functionalized with polyethylene glycol and targeting ligands such as folic acid to improve their biocompatibility and target specificity, respectively. The effectiveness of these porphyrin-based hybrid nanomaterials was successfully demonstrated in vitro using cervical human cancer (HeLa), lung (H-460) and colon (HT-29) cancer cells. http://dx.doi.org/10.1016/j.pdpdt.2015.07.062 Targeted photodynamic therapy with colon cancer-specific peptide conjugated photosensitizer Ju Hee Kim 1 , Yoon Jin Roh 1 , In-Wook Kim 1 , Hyun-A Kim 1 , Jae Myung Park 1 , Tayyaba Hasan 2 , Myung-Gyu Choi 1 1
Catholic-Harvard Wellman Photomedicine Center, Division of Gastroenterology, The Catholic University of Korea, Seoul, Republic of Korea 2 Wellman Center for Photomedicine, Department of Dermatology, Massachusetts General Hospital, Harvard School, Boston, USA Colon cancer is one of the most commonly diagnosed cancers in the world. In numerous cases, colon cancer is derived from pre-malignant lesion, and the present diagnostic methods with white light endoscopy have limits for detecting all pre-cancer lesion. Therefore, improved approaches for detecting pre-cancer lesion are needs. We aim to develop probe possible to detect and treat the small lesion at the same time. To isolate the peptide specific to colon cancer, we screened phage display peptide libraries against human colon cancer cell lines. Analyzed peptide sequences were synthesized, and then conjugated with fluorescence or photosensitizer. Immunocytochemical staining using FITC or photosensitizer-conjugated peptides showed high level of binding affinity to colon cancer cell lines. We also saw no binding
Microfluidic study of targeted imaging and photodynamic therapy Nishanth Venugopal Menon 1,2 , Sivaramapanicker Sreejith 1,2 , Yanli Zhao 1,2 , Yuejun Kang 1,2 1
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 2 Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore We have demonstrated selective targeted imaging and photodynamic therapy (PDT) aspects of a micelle encapsulated near-infrared dye in a microfluidic device. An NIR squaraine photosensitizer was selected that have been reported to have the potential to generate singlet oxygen and hence a potential photosensitizer in photodynamic therapy. The dye was encapsulated in micelles modified with folic acid (folate receptor (FR) targeting). A three chamber custom made microfluidic device was fabricated and studies were performed. HeLa (FR positive) was co-cultured individually with FR negative cell lines; human bone marrow stem cells (BMSCs), bone marrow stroma (HS5) and hepatocarcinoma (HUH7) within a microfluidic chip to demonstrate the effectiveness of the photosensitizer. After a 20 min irradiation at 660 nm, more than 60% cell death could be observed in the HeLa cells at the end of 24 h while the FR negative cells appeared to be growing well. The size and photo-physical characterization of the micelle was performed to better understand its functioning and its ability to generate singlet oxygen was confirmed using anthracene-9,10-diyl-bis-methylmalonate (ADMA). A novel technique of photosensitizer modification has been discussed here and we believe that such a modification could lead to more targeted PDT. http://dx.doi.org/10.1016/j.pdpdt.2015.07.064 Hypericinates preparation and comparative study of cell viability with hypericin Gislaine Patricia de Andrade, Giselle Cerchiaro, Anderson Orzari Ribeiro Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Brazil Photodynamic Therapy (PDT) is a technique based on topical or systemic administration of a photosensitizer, which accumulate in the diseased tissue. When exposed to light, the photosensitizer generates reactive oxygen species leading to cell inactivation or death. Hypericin, a third generation photosensitizer, has potent photosensitizing activity, but due to its hydrophobic macrocycle, it has low solubility in biological environment, making it difficult to use in photodynamic therapy. Hypericinates (hypericin salts) are compounds formed from deprotonation of hydroxyl groups located. http://dx.doi.org/10.1016/j.pdpdt.2015.07.065
Abstracts / Photodiagnosis and Photodynamic Therapy 12 (2015) 325–375
Multifunctional porphyrin-based polysilsesquioxane nanoparticles with improved loading capacity and phototherapeutic effect Juan L. Vivero-Escoto 1,2 , Zachary Lyles 1,2 , Daniel L. Vega 1,2
the peptides to normal colon cell line. We confirmed that peptide conjugated photosensitizer had enhanced photodynamic therapy (PDT) effect compared to none conjugated photosensitizer. The results from this study suggest that targeted PDT using peptide probe may be a promising candidate drug in the development of a useful colon cancer diagnosis and treatment. http://dx.doi.org/10.1016/j.pdpdt.2015.07.063
1
Department of Chemistry, University of North Carolina at Charlotte, Charlotte, NC 28223, USA 2 The Center for Biomedical Engineering and Science, University of North Carolina at Charlotte, Charlotte, NC 28223, USA The application of nanoparticles as delivery platforms in PDT is a promising alternative for resolving some of the major challenges associated with classic photosensitizers (PSs) such as porphyrin molecules. Polysilsesquioxane nanoparticles (PSQ-NPs) are crosslinked homopolymers formed by the condensation of functionalized trialkoxysilanes. Recent reports have shown that PSQ-NPs can provide an interesting platform for developing PS nanocarriers. Several advantages can be foreseen by using this platform such as carrying a large payload of PS molecules; their surface and composition can be tailored to develop multifunctional systems. In this work, two PSQ-NP systems with a high payload of photosensitizers were synthesized, characterized, and applied in vitro. The network of these nanomaterials is formed by two different types of porphyrin-based photosensitizers, protoporphyrin-IX and tetrakis(4-carboxyphenyl)porphyrin, chemically connected via a redox-responsive linker. Both platforms can be further functionalized with polyethylene glycol and targeting ligands such as folic acid to improve their biocompatibility and target specificity, respectively. The effectiveness of these porphyrin-based hybrid nanomaterials was successfully demonstrated in vitro using cervical human cancer (HeLa), lung (H-460) and colon (HT-29) cancer cells. http://dx.doi.org/10.1016/j.pdpdt.2015.07.062 Targeted photodynamic therapy with colon cancer-specific peptide conjugated photosensitizer Ju Hee Kim 1 , Yoon Jin Roh 1 , In-Wook Kim 1 , Hyun-A Kim 1 , Jae Myung Park 1 , Tayyaba Hasan 2 , Myung-Gyu Choi 1 1
Catholic-Harvard Wellman Photomedicine Center, Division of Gastroenterology, The Catholic University of Korea, Seoul, Republic of Korea 2 Wellman Center for Photomedicine, Department of Dermatology, Massachusetts General Hospital, Harvard School, Boston, USA Colon cancer is one of the most commonly diagnosed cancers in the world. In numerous cases, colon cancer is derived from pre-malignant lesion, and the present diagnostic methods with white light endoscopy have limits for detecting all pre-cancer lesion. Therefore, improved approaches for detecting pre-cancer lesion are needs. We aim to develop probe possible to detect and treat the small lesion at the same time. To isolate the peptide specific to colon cancer, we screened phage display peptide libraries against human colon cancer cell lines. Analyzed peptide sequences were synthesized, and then conjugated with fluorescence or photosensitizer. Immunocytochemical staining using FITC or photosensitizer-conjugated peptides showed high level of binding affinity to colon cancer cell lines. We also saw no binding
Microfluidic study of targeted imaging and photodynamic therapy Nishanth Venugopal Menon 1,2 , Sivaramapanicker Sreejith 1,2 , Yanli Zhao 1,2 , Yuejun Kang 1,2 1
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 2 Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore We have demonstrated selective targeted imaging and photodynamic therapy (PDT) aspects of a micelle encapsulated near-infrared dye in a microfluidic device. An NIR squaraine photosensitizer was selected that have been reported to have the potential to generate singlet oxygen and hence a potential photosensitizer in photodynamic therapy. The dye was encapsulated in micelles modified with folic acid (folate receptor (FR) targeting). A three chamber custom made microfluidic device was fabricated and studies were performed. HeLa (FR positive) was co-cultured individually with FR negative cell lines; human bone marrow stem cells (BMSCs), bone marrow stroma (HS5) and hepatocarcinoma (HUH7) within a microfluidic chip to demonstrate the effectiveness of the photosensitizer. After a 20 min irradiation at 660 nm, more than 60% cell death could be observed in the HeLa cells at the end of 24 h while the FR negative cells appeared to be growing well. The size and photo-physical characterization of the micelle was performed to better understand its functioning and its ability to generate singlet oxygen was confirmed using anthracene-9,10-diyl-bis-methylmalonate (ADMA). A novel technique of photosensitizer modification has been discussed here and we believe that such a modification could lead to more targeted PDT. http://dx.doi.org/10.1016/j.pdpdt.2015.07.064 Hypericinates preparation and comparative study of cell viability with hypericin Gislaine Patricia de Andrade, Giselle Cerchiaro, Anderson Orzari Ribeiro Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Brazil Photodynamic Therapy (PDT) is a technique based on topical or systemic administration of a photosensitizer, which accumulate in the diseased tissue. When exposed to light, the photosensitizer generates reactive oxygen species leading to cell inactivation or death. Hypericin, a third generation photosensitizer, has potent photosensitizing activity, but due to its hydrophobic macrocycle, it has low solubility in biological environment, making it difficult to use in photodynamic therapy. Hypericinates (hypericin salts) are compounds formed from deprotonation of hydroxyl groups located. http://dx.doi.org/10.1016/j.pdpdt.2015.07.065