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Hypersensitivity pneumonitis to chikcens in horses
VOLUME 51
NUMBER2
Abstracts of papers
103
ejaculate. Total ejaculates from all of 3 different normal males and from all of 12 males with vasectomies gave positive wheal-and-flare reactions at dilutions of l:10e-107, These dilutions were nonirritating. (2) Pooled seminal plasma was fraetionated on Sephadex G-200, and 8 fractions were tested; 90 per cent + of activity (W & F) was in a fraction corresponding to 10,000 to 15,000 Dalton's M.W. This fraction has a U.V. spectrum resembling protein and activity to 10-3 #g per milliliter. (3) Specific histamine release from the patient's whole blood with 1:105 dilution seminal plasma and the active fraction was obtained (27 per cent release vs. 0.6 per cent from normal whole blood). Patient's serum did not agglutinate washed sperm but agglutinated sperm in whole ejaculate. Results show immediate hypersensitivity to a 10,000 to 15,000 M.W. protein in human seminal plasma mediated (at least in part) by a homocytotropie antibody specific for this material.
52. Hypersensitivity pneumonitis to chickens in horses. Richard A. Mansmann, V . M . D . , a n d B e n n i e I. O s b u r n , D . V . M . , P h . D . , D a v i s , C a l i f . Chronic bronehiolitis (heaves) in horses has been suspected of being of immunologic origin; however, the implication of specific antigen(s) in the disease has not been defined. This report describes the results of immunologic studies on 2 horses from different stables which had a clinical history of heaves and a close association with chickens. Immunologic studies revealed that both horses had the following reactions with chicken serum: (1) significant intradermal skin reactions at 4 hours which (2) histologically appeared as a neutrophilic vasculitis, (3) precipitating antibodies in serum, and (4) after nebulization one horse developed clinical signs by 4 hours, while an amnestic response was observed in the other horse. Horse ], which did not develop respiratory distress after nebulization with chicken sertml, had not been exposed to chickens for 2 months and had been asymptomatic during this period. Horse 2, challenged by nebulization with chicken serum and saline extract of chicken feces immediately after becoming asymptomatic after removal from his stable, developed dyspnea within 4 hours. Clinically Horse 1 has been normal since chickens were removed from its environment, and the precipitating antibody levels continued to decline. The lesions observed at necropsy in Horse 2 included a prominent peribronchiolitis consisting primarily of mononuclear cells and smooth muscle hyperplasia. There was no evidence of emphysema. These cases suggest that antigens associated with chickens are capable of causing an immune complex respiratory disease (heaves) in the horse that resembles in many respects ~some of the hypersensitivity pneumonitides of man.
,53. Scanning electron microscopy of acute experimental hypersensitivity pneumonitis in rabbits. A l l e n R . T h o m a s , B . A . , a n d H a l B. R i c h e r s o n , M.D., Iowa City, Iowa Scanning electron microscopy was utilized to study pulmonary lesions in immunized rabbits following the inhalation of aerosolized antigen. New Zealand white rabbits were immunized by toe pad injection of either 5• crystallized ovalbumin or horse spleen ferritin in complete Frcund's adjuvant. The animals were challenged E. weeks later with ultrasonically nebulized antigen as described previously (Am. Rev. Resp. Dis. 1{)4: 568, 1971). Pulmonary histopathology was studied 48 hours post-challenge using scanning and transmission electron microscopy as well as routine light microscopy. Scanning electron microscopy of lung sections from immunized-challenged rabbits revealed t:~ree-dimensional changes in architecture that complement those of light microscopy. Spotty cellular filling of alveeli and thickening of alveolar septa were consistent in experimental animals. Alveolar lesions occurred predominantly in locations just distal to terminal bronc:~ioles. Cells packing the alveoli were large round cells with variable extruding processes. Preliminary transmission microscopy indicates that the dominant cells filling the alveoli are
NUMBER2
Abstracts of papers
103
ejaculate. Total ejaculates from all of 3 different normal males and from all of 12 males with vasectomies gave positive wheal-and-flare reactions at dilutions of l:10e-107, These dilutions were nonirritating. (2) Pooled seminal plasma was fraetionated on Sephadex G-200, and 8 fractions were tested; 90 per cent + of activity (W & F) was in a fraction corresponding to 10,000 to 15,000 Dalton's M.W. This fraction has a U.V. spectrum resembling protein and activity to 10-3 #g per milliliter. (3) Specific histamine release from the patient's whole blood with 1:105 dilution seminal plasma and the active fraction was obtained (27 per cent release vs. 0.6 per cent from normal whole blood). Patient's serum did not agglutinate washed sperm but agglutinated sperm in whole ejaculate. Results show immediate hypersensitivity to a 10,000 to 15,000 M.W. protein in human seminal plasma mediated (at least in part) by a homocytotropie antibody specific for this material.
52. Hypersensitivity pneumonitis to chickens in horses. Richard A. Mansmann, V . M . D . , a n d B e n n i e I. O s b u r n , D . V . M . , P h . D . , D a v i s , C a l i f . Chronic bronehiolitis (heaves) in horses has been suspected of being of immunologic origin; however, the implication of specific antigen(s) in the disease has not been defined. This report describes the results of immunologic studies on 2 horses from different stables which had a clinical history of heaves and a close association with chickens. Immunologic studies revealed that both horses had the following reactions with chicken serum: (1) significant intradermal skin reactions at 4 hours which (2) histologically appeared as a neutrophilic vasculitis, (3) precipitating antibodies in serum, and (4) after nebulization one horse developed clinical signs by 4 hours, while an amnestic response was observed in the other horse. Horse ], which did not develop respiratory distress after nebulization with chicken sertml, had not been exposed to chickens for 2 months and had been asymptomatic during this period. Horse 2, challenged by nebulization with chicken serum and saline extract of chicken feces immediately after becoming asymptomatic after removal from his stable, developed dyspnea within 4 hours. Clinically Horse 1 has been normal since chickens were removed from its environment, and the precipitating antibody levels continued to decline. The lesions observed at necropsy in Horse 2 included a prominent peribronchiolitis consisting primarily of mononuclear cells and smooth muscle hyperplasia. There was no evidence of emphysema. These cases suggest that antigens associated with chickens are capable of causing an immune complex respiratory disease (heaves) in the horse that resembles in many respects ~some of the hypersensitivity pneumonitides of man.
,53. Scanning electron microscopy of acute experimental hypersensitivity pneumonitis in rabbits. A l l e n R . T h o m a s , B . A . , a n d H a l B. R i c h e r s o n , M.D., Iowa City, Iowa Scanning electron microscopy was utilized to study pulmonary lesions in immunized rabbits following the inhalation of aerosolized antigen. New Zealand white rabbits were immunized by toe pad injection of either 5• crystallized ovalbumin or horse spleen ferritin in complete Frcund's adjuvant. The animals were challenged E. weeks later with ultrasonically nebulized antigen as described previously (Am. Rev. Resp. Dis. 1{)4: 568, 1971). Pulmonary histopathology was studied 48 hours post-challenge using scanning and transmission electron microscopy as well as routine light microscopy. Scanning electron microscopy of lung sections from immunized-challenged rabbits revealed t:~ree-dimensional changes in architecture that complement those of light microscopy. Spotty cellular filling of alveeli and thickening of alveolar septa were consistent in experimental animals. Alveolar lesions occurred predominantly in locations just distal to terminal bronc:~ioles. Cells packing the alveoli were large round cells with variable extruding processes. Preliminary transmission microscopy indicates that the dominant cells filling the alveoli are