- Email: [email protected]
Hypothalamic histamine modulates delayed suppression of feeding following infusion of 2-deoxy-D-glucose
S148
HYPOTHALAMICHISTAMINEMODULATES DELAYED SUPPRESSION OF FEEDING FOLLOWING INFUSIONOF 2-DEOXY-D-GLUCOSE. YUICHI TAMARI. HIRONOBU YOSHIMATSU.MAMORU KUROKAWA.AKIHIKO OOHARA. TAKAKO FUJISE. AND TOSHIIE SAKATA. Departmentof InternalMedicine I, Facults of Medicine.Ksushu University.8-l-l Maidashi.Hinashi-ku.Fukuoka 812. Jauan. Central administration of 2-deoxy-D-glucose (2-DG) has been shown to induce initial elicitation of feeding and delayed suppression of feeding. To clarify effects of hypothalamic histamine on the suppression, we investigated effects of 2-DG on hypothalamic levels of histamine (HA) and tele-methylhistamine(t-MH), a major metaboliteof HA, to evaluate activity of histaminernicsystems in rats. To match with the behavioral responces, leveis of HA and t-MH in the hypothalamus were evaluatedby high performanceliquid chromatography 7 hrs after infusionof 2-DG into the third cerebroventricle(icv).Contents of hypothalamicHA and t-MH increased correspondinglywith feeding-suppression (p
PREOPTIC EFFERENTS SUPPRESS THE DISPLAY OF LORDOSISREFLEX IN THE FEMALE RAT TERUKO TAKEO, AND YASUO SAKUMA, Departmentof Physiology1, HirosakiUniversitySchool of Medicine,5 Zaifucho, Hirosaki036, Japan. of the medial preopticarea (POA) from the septum by In the ovariectomized rats, disconnection knife cuts reduced the amount of estrogen needed to elicit lordosis reflex, an estrogendependentprincipalelementof female sexual behavior,from 15ug to 1.5ug. Despite the removalof septal inhibition,bilateralor unilateralelectricalstimulationof the deafferentedPOA through chronicallyimplanted electrodescaused a prompt and strong suppressionof lordosis in free moving,estrogen-and progesterone-treated ovariectomized female rats in responseto male mounts. The reflex interrupted gradually to increasedstimulusintensity,with a thresholdat 30 uA. The optimal frequencywas at 50-100 Hz. After the terminationof electricalstimulationof the deafferentedPOA, lordosisperformancereturnedpromptlyto the pre-stimulation level. The rapid time course distinguishedthe stimulus-boundinhibitionof lordosisin the transectedrats from that in the sham-operatedanimals,in which slow onset and recoverycharacterizedthe response. Electricalstimulationspecificallyblockedlordosis,without disruptingoroceptivecomponentsof the female sexual behavior.These results suggest that POA appears to be a separateentity in the inhibitorymechanismof lordosis,independentof the septum.The exaggeratedresponseto the stimulationof the deafferentedPOA suggests eliminationof facilitatoryneural component for this reflex by the knife cut.
CHANGES OF LACTATE CONCENTRATIONSIN HIPPOCAMPUSAND CAUOATE PUTAWEN AFTER VARIOUS STRESSES IN FREELY WOVING RATS. KOUICHI KURATA', HERWAN G.W. WESTENBERG' FOKKO J.BOSKER*, AND ANDRE KLOMPkLARKERS_~,_ 'Departmentof _I _...__.___ Neuropsychiatry,Faculty of Rdicine. Toyama Wedical and PharmaceuticalUnivels_i_tyl__20ep_ar_tnent of Biological Psychiatry,Utrecht University,'Sugitani2630 Toy@11~_930:61,~ Japaa, 2Utrecht,_The_Netherlandp. ___~_ A Continuous monitoring of lactate concentration,lactography,was examined in the hippocampus (HP) and the caudate putamen (CPU) of a freely moving rat after two kinds of stresses using a microdialysismethod. Each rat was exposed to 5 rin of a changing cage stress and to two times of 5 min ice bath stress. A basal lactate output was 50-lOOtiM in the HP and the CPU. A lactogramafter the changing cage showed 20% of the maximum increase at 5-10min point and returned to the basal level at 15min point in the HP and the CPU. The 1st and the 2nd ice bath stresses induced 45-50X increase of laclate at 5-8 min point in the HP . There was no difference between the 1st and the 2nd experiments with respect to the values of the maximum increase. However, the elimination rate of lactate in the 2nd was larger than that in the 1st. The 1st and the 2nd ice bath stresses induced 30% increase of the lactate output at 5-6min point and retured to the basal level at 20 min point in the CPU. There was no difference between the 1st and the 2nd experiments concerning values of the maximum increase and the elimination rate in the CPU in contrast to the HP. Every stress was sensitive to 2OctH of tetrodotoxin. This result shows that the lactography is a sensitive indicator of a neuronal activity.
HYPOTHALAMICHISTAMINEMODULATES DELAYED SUPPRESSION OF FEEDING FOLLOWING INFUSIONOF 2-DEOXY-D-GLUCOSE. YUICHI TAMARI. HIRONOBU YOSHIMATSU.MAMORU KUROKAWA.AKIHIKO OOHARA. TAKAKO FUJISE. AND TOSHIIE SAKATA. Departmentof InternalMedicine I, Facults of Medicine.Ksushu University.8-l-l Maidashi.Hinashi-ku.Fukuoka 812. Jauan. Central administration of 2-deoxy-D-glucose (2-DG) has been shown to induce initial elicitation of feeding and delayed suppression of feeding. To clarify effects of hypothalamic histamine on the suppression, we investigated effects of 2-DG on hypothalamic levels of histamine (HA) and tele-methylhistamine(t-MH), a major metaboliteof HA, to evaluate activity of histaminernicsystems in rats. To match with the behavioral responces, leveis of HA and t-MH in the hypothalamus were evaluatedby high performanceliquid chromatography 7 hrs after infusionof 2-DG into the third cerebroventricle(icv).Contents of hypothalamicHA and t-MH increased correspondinglywith feeding-suppression (p
PREOPTIC EFFERENTS SUPPRESS THE DISPLAY OF LORDOSISREFLEX IN THE FEMALE RAT TERUKO TAKEO, AND YASUO SAKUMA, Departmentof Physiology1, HirosakiUniversitySchool of Medicine,5 Zaifucho, Hirosaki036, Japan. of the medial preopticarea (POA) from the septum by In the ovariectomized rats, disconnection knife cuts reduced the amount of estrogen needed to elicit lordosis reflex, an estrogendependentprincipalelementof female sexual behavior,from 15ug to 1.5ug. Despite the removalof septal inhibition,bilateralor unilateralelectricalstimulationof the deafferentedPOA through chronicallyimplanted electrodescaused a prompt and strong suppressionof lordosis in free moving,estrogen-and progesterone-treated ovariectomized female rats in responseto male mounts. The reflex interrupted gradually to increasedstimulusintensity,with a thresholdat 30 uA. The optimal frequencywas at 50-100 Hz. After the terminationof electricalstimulationof the deafferentedPOA, lordosisperformancereturnedpromptlyto the pre-stimulation level. The rapid time course distinguishedthe stimulus-boundinhibitionof lordosisin the transectedrats from that in the sham-operatedanimals,in which slow onset and recoverycharacterizedthe response. Electricalstimulationspecificallyblockedlordosis,without disruptingoroceptivecomponentsof the female sexual behavior.These results suggest that POA appears to be a separateentity in the inhibitorymechanismof lordosis,independentof the septum.The exaggeratedresponseto the stimulationof the deafferentedPOA suggests eliminationof facilitatoryneural component for this reflex by the knife cut.
CHANGES OF LACTATE CONCENTRATIONSIN HIPPOCAMPUSAND CAUOATE PUTAWEN AFTER VARIOUS STRESSES IN FREELY WOVING RATS. KOUICHI KURATA', HERWAN G.W. WESTENBERG' FOKKO J.BOSKER*, AND ANDRE KLOMPkLARKERS_~,_ 'Departmentof _I _...__.___ Neuropsychiatry,Faculty of Rdicine. Toyama Wedical and PharmaceuticalUnivels_i_tyl__20ep_ar_tnent of Biological Psychiatry,Utrecht University,'Sugitani2630 Toy@11~_930:61,~ Japaa, 2Utrecht,_The_Netherlandp. ___~_ A Continuous monitoring of lactate concentration,lactography,was examined in the hippocampus (HP) and the caudate putamen (CPU) of a freely moving rat after two kinds of stresses using a microdialysismethod. Each rat was exposed to 5 rin of a changing cage stress and to two times of 5 min ice bath stress. A basal lactate output was 50-lOOtiM in the HP and the CPU. A lactogramafter the changing cage showed 20% of the maximum increase at 5-10min point and returned to the basal level at 15min point in the HP and the CPU. The 1st and the 2nd ice bath stresses induced 45-50X increase of laclate at 5-8 min point in the HP . There was no difference between the 1st and the 2nd experiments with respect to the values of the maximum increase. However, the elimination rate of lactate in the 2nd was larger than that in the 1st. The 1st and the 2nd ice bath stresses induced 30% increase of the lactate output at 5-6min point and retured to the basal level at 20 min point in the CPU. There was no difference between the 1st and the 2nd experiments concerning values of the maximum increase and the elimination rate in the CPU in contrast to the HP. Every stress was sensitive to 2OctH of tetrodotoxin. This result shows that the lactography is a sensitive indicator of a neuronal activity.