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Identification and analysis of a cuticular collagen-encoding gene from the plant-parasitic nematode meloidogyne incognita
Gene, 155 (1995) 145-146 © 1995 Elsevier Science B.V. All rights reserved. 0378-1119/95/$09.50
145
Erratum Gene, 151 (1994) 237-242 © 1994 Elsevier Science B.V. All rights reserved. 0378-1119/94/$07.00
G E N E 08348
Identification and analysis of a cuticular collagen-encoding gene from the plant-parasitic nematode Meloidogyne incognita (Cuticle; differential screening; hypodermis; polymerase chain reaction; root knot; tomato)
Walter Van der Eycken, Janice de Almeida Engler, Marc Van Montagu and Godelieve Gheysen Laboratorium voor Genetica, Universiteit Gent, B-9000 Gent, Belgium
Received by G.N. Godson: 24 February 1994; Revised/Accepted: 4 May/30 June 1994; Received at publishers: 15 August 1994
In the above-mentioned article, Fig. 6 was printed in black and white, whereas it should have been in colour. The correct Fig. 6 appears on the next page. The Publisher apologises for any inconvenience caused.
Correspondence to: Dr. M. Van Montagu, Laboratorium voor Genetica, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium. Tel. (32-9) 264-5170; Fax (32-9) 264-5349; e-mail: [email protected]
Abbreviations: A., Ascaris; aa, amino acid(s); bp, base pair(s); C., Caenorhabditis; cDNA, DNA complementary to RNA; col, gene(s) encoding collagen; D., Ditylenchus; H., Haemonchus; kb, kilobase(s) or 1000 bp; Lemmi, Lycopersicon esculentum cv. Marmande M. incognita; Lemmi, protein produced by Lemmi gene; Lemmi 5, col cDNA from Lemmi; M., Meloidogyne; nt, nucleotide(s); oligo, oligodeoxyribonucleotide; PAGE, polyacrylamide-gel electrophoresis; PCR, polymerase chain reaction; SDS, sodium dodecyl sulfate; SSC, 150mM NaC1/15mM Na3.citrate pH7.0; TMAC, tetramethylammoniumchloride; Xaa (Yaa), any aa.
146
A
C
D h
n
c~,
Fig. 6. In situ hybridization of Lemmi 5 probes to a section of M. incognita-infected tomato root. Panel A shows a schematic drawing of a tissue section through a tomato root gall induced by M. incognita. Panel B is a photograph of a similar toluidine-stained section. Panel C shows a dark field picture of a section through a gall, hybridized with a Lemmi 5 antisense probe. The bright superficial region of the nematode (hypodermis) represents the hybridization signal. The cuticle is partially released from the underlying hypodermis. Panel D illustrates that a Lemmi 5 sense probe did not hybridize to a similar section. The blue color in the sections at panel C and especially D, is from toluidine staining, a, artificial cavity due to fixation; c, somewhat released cuticle; co, cortical cells; g, multinucleate giant cell; h, hypodermis; n, nematode; p, pericycle cells; x, xylem. The bar represents 100 p.m. Methods; In vitro grown A. rhizogenes-transformed tomato roots were inoculated with L2 larvae. 4-week-old galls were used to perform in situ hybridizations which were carried out essentially as described by Angerer and Angerer (1921). R N A probes, labeled with 35S, were synthesized from a clone of the Lernmi 5 c D N A in the plasmid p G e m 2 (Promega, Madison, WI, USA). Photographs were taken with a Leitz Diaplan microscope equipped with dark-field optics.
145
Erratum Gene, 151 (1994) 237-242 © 1994 Elsevier Science B.V. All rights reserved. 0378-1119/94/$07.00
G E N E 08348
Identification and analysis of a cuticular collagen-encoding gene from the plant-parasitic nematode Meloidogyne incognita (Cuticle; differential screening; hypodermis; polymerase chain reaction; root knot; tomato)
Walter Van der Eycken, Janice de Almeida Engler, Marc Van Montagu and Godelieve Gheysen Laboratorium voor Genetica, Universiteit Gent, B-9000 Gent, Belgium
Received by G.N. Godson: 24 February 1994; Revised/Accepted: 4 May/30 June 1994; Received at publishers: 15 August 1994
In the above-mentioned article, Fig. 6 was printed in black and white, whereas it should have been in colour. The correct Fig. 6 appears on the next page. The Publisher apologises for any inconvenience caused.
Correspondence to: Dr. M. Van Montagu, Laboratorium voor Genetica, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium. Tel. (32-9) 264-5170; Fax (32-9) 264-5349; e-mail: [email protected]
Abbreviations: A., Ascaris; aa, amino acid(s); bp, base pair(s); C., Caenorhabditis; cDNA, DNA complementary to RNA; col, gene(s) encoding collagen; D., Ditylenchus; H., Haemonchus; kb, kilobase(s) or 1000 bp; Lemmi, Lycopersicon esculentum cv. Marmande M. incognita; Lemmi, protein produced by Lemmi gene; Lemmi 5, col cDNA from Lemmi; M., Meloidogyne; nt, nucleotide(s); oligo, oligodeoxyribonucleotide; PAGE, polyacrylamide-gel electrophoresis; PCR, polymerase chain reaction; SDS, sodium dodecyl sulfate; SSC, 150mM NaC1/15mM Na3.citrate pH7.0; TMAC, tetramethylammoniumchloride; Xaa (Yaa), any aa.
146
A
C
D h
n
c~,
Fig. 6. In situ hybridization of Lemmi 5 probes to a section of M. incognita-infected tomato root. Panel A shows a schematic drawing of a tissue section through a tomato root gall induced by M. incognita. Panel B is a photograph of a similar toluidine-stained section. Panel C shows a dark field picture of a section through a gall, hybridized with a Lemmi 5 antisense probe. The bright superficial region of the nematode (hypodermis) represents the hybridization signal. The cuticle is partially released from the underlying hypodermis. Panel D illustrates that a Lemmi 5 sense probe did not hybridize to a similar section. The blue color in the sections at panel C and especially D, is from toluidine staining, a, artificial cavity due to fixation; c, somewhat released cuticle; co, cortical cells; g, multinucleate giant cell; h, hypodermis; n, nematode; p, pericycle cells; x, xylem. The bar represents 100 p.m. Methods; In vitro grown A. rhizogenes-transformed tomato roots were inoculated with L2 larvae. 4-week-old galls were used to perform in situ hybridizations which were carried out essentially as described by Angerer and Angerer (1921). R N A probes, labeled with 35S, were synthesized from a clone of the Lernmi 5 c D N A in the plasmid p G e m 2 (Promega, Madison, WI, USA). Photographs were taken with a Leitz Diaplan microscope equipped with dark-field optics.