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IgE Anti-Respiratory Syncytial Virus Antibodies In Older Asthmatic Children
Abstracts AB285
J ALLERGY CLIN IMMUNOL VOLUME 133, NUMBER 2
Effect Of Prenatal Exposure To Indoor PM2.5 and Environmental Tobacco Smoke Affecting Lower Respiratory Tract Infection Was Modified By ROS Genes: Cocoa Study Dr. Song I. Yang, MD1, Dr. Eun Lee, MD1, Dr. Young Ho Jung, MD1, Kil-Yong Choi2, Mi-Jin Kang, MS2, Ho-Sung Yu2, Cheol Min Lee3, Prof. Youn Ho Shin, MD, PhD4, Prof. Kangmo Ahn, MD, PhD5, Prof. Kyung Won Kim, MD, PhD6, Prof. Soo-Jong Hong, MD, PhD1 Cocoa study Group7; 1Childhood Asthma Atopy Center, Department of Pediatrics, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea, 2Asan Institute for Life Sciences, University of Ulsan College of Medicine, Seoul, South Korea, 3Institute of Environmental and Industrial Medicine, Hanyang University, Seoul, South Korea, 4 Department of Pediatrics, CHA Medical Center, CHA University College of Medicine, Seoul, South Korea, 5Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea, 6Department of Pediatrics, Yonsei University College of Medicine, Seoul, South Korea, 7COCOA study group. RATIONALE: Prenatal air pollution exposure associates with impaired fetal growth, decreased lung function, respiratory morbidity and mortality. We aimed to investigate the influence of prenatal indoor fine particulate matter (PM2.5) and environmental tobacco smoke (ETS) exposure and genetic polymorphisms on the susceptibility to respiratory tract infection (RTI) in infancy. METHODS: Demographic, environmental factors and diagnosis of RTI in the first year of life were evaluated in 231 infants from a birth cohort of general population in Korea between 2007 and 2011. Indoor PM2.5 was measured during the third trimester and mothers were asked about ETS exposure during pregnancy. Nuclear factor (erythroid-derived 2)-like 2 (NRF2, rs6726395), glutathione S-transferase pi 1 (GSTP1, rs1695) and glutathione S-transferase mu 1 (GSTM1, copy number variation) genotyping was performed. RESULTS: Prenatal indoor PM2.5 or ETS exposure increased the risk of bronchiolitis and lower RTI (LRTI), but not upper RTI (URTI), in infancy. They also synergistically increased susceptibility to bronchiolitis and LRTI (aOR 5.20; 95% CI 1.10-24.61, aOR 5.18; 95% CI 1.40-19.24, respectively), but not URTI. This synergistic effect was increased by Nrf2 GG (aOR 10.23; 95% CI 1.02-102.57, aOR 14.01; 95% CI 1.42-138.02, respectively) and GSTM1 null genotypes (aOR 9.45; 95% CI 1.03-86.78, aOR 5.70; 95% CI 1.02-31.95, respectively). CONCLUSIONS: Indoor PM2.5 and ETS exposure during the prenatal period synergistically increased susceptibility to bronchiolitis and LRTI, but not URTI. This effect was modified by polymorphisms in reactive oxygen species-related genes.
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IgE Anti-Respiratory Syncytial Virus Antibodies In Older Asthmatic Children Mira Mandal1, Dr. Rauno O. Joks, MD, FAAAAI2, Dr. Kevin Norowitz, MD3, Dr. Diana Weaver, MD4, Dr. Helen G. Durkin, PhD5, Dr. Martin H. Bluth, MD, PhD6, Dr. Stephan Kohlhoff, MD5, Dr. Tamar A. Smith-Norowitz, PhD7; 1SUNY Downstate Medical Center, 2 SUNY-HSC, Brooklyn, NY, 3SUNY Downstate Medical Center, Center for Allergy and Asthma Research, Brooklyn, NY, 4SUNY Downstate Med. Ctr, Brooklyn, NY, 5Center for Allergy and Asthma Research, State University of New York Downstate Medical Center, Brooklyn, NY, 6 Wayne State University Medicine, Detroit, MI, 7Center for Allergy and Asthma Research at SUNY Downstate, Brooklyn, NY. RATIONALE: Respiratory syncytial virus (RSV) causes severe lower respiratory tract disease in infant and young children, and is a serious public health concern, as is the increase in pediatric asthma in the United States. However, it remains unknown if IgE and RSVantibodies (Abs) play a role in development of asthma. METHODS: Total serum IgE, and IgE and IgG anti-RSV Ab responses were studied in older asthmatic compared with non asthmatic children (M/ F, mean age: 14) (N5 30, N543, respectively) (UniCAP total IgE
Fluoroenzymeimmunoassay (IU/mL), enzyme-linked immunosorbent assay (U/mL; OD value). RESULTS: Total serum IgE levels were increased 3-fold in asthmatic compared with non asthmatic children (323 + 217, 107 + 116, P5 0.0003). Further, IgE anti-RSV Ab levels were 1.5-fold higher in asthmatic compared with nonasthmatic children (P 5 0.01); IgG anti-RSVAb levels were slightly increased in the asthmatic children (112 + 32, 81+ 59, P50.003). Interestingly, values of IgE anti-RSV Abs positively correlated with IgG anti-RSV Abs. CONCLUSIONS: The presence of IgE anti-RSV abs in older children further lends support to an important role of RSV infection in asthma.
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Asthma Symptoms and Rhinovirus In A Longitudinal Children's Cohort Dr. Euan R. Tovey, PhD1,2, Dr. S. Stelzer-Braid, PhD3,4, Dr. B. G. Toelle, PhD1, Ms. C. M. Willenborg, BSc3, Dr. H. K. Reddel, PhD, MBBS1,2, Ms. F. L. Garden, MPH1,2, Prof. A. Jaffe, PhD, MD4,5, Ms. R. Strachan5, Dr. B. G. Oliver, PhD1,2, Dr. Y. C. Belessis, MBBS4,5, Prof. G. B. Marks, PhD, MD1,2, Prof. W. D. Rawlinson, PhD, MD3,4; 1 Woolcock Institute of Medical Research, Sydney, Australia, 2University of Sydney, Australia, 3Virology Division, SEALS Microbiology, Prince of Wales Hospital, Sydney, Australia, 4University of NSW, Australia, 5 Sydney Children’s Hospital, Randwick Australia. RATIONALE: Emergency presentations for asthma are strongly associated with rhinovirus (HRV) infections in children. However, community viral infections are common and often mild or asymptomatic, and the role of viruses in changing daily symptoms is less clear. We hypothesized such infections would be associated with increased symptoms. METHODS: 67 children, aged 5-12, with moderately severe asthma, selfcollected nasal-wash and exhaled breath samples and recorded their recent asthma and cold symptoms and current lung function, twice per week for 10 weeks. The presence of 8 viruses, including rhinovirus (HRV), was analysed by PCR. A mixed model, to account for repeated measures, was used to determine the current and delayed impact of viruses on symptoms and lung function. RESULTS: 25.5% of nasal samples and 11.5% of breath samples were positive for HRV; only 1.8% were positive for other viruses. The presence of HRV in nasal wash, but not in breath, was associated with the presence of symptoms; adjusted odds ratios: cough 2.53, (95%CI 1.62-3.94), wheeze 3.05, (95%CI 1.89 to 4.93), self-reported febrile 2.07, (95%CI 1.17 to 3.64) and coryzal symptoms 1.95, (95%CI 1.14 to 3.32) over the previous 3-4 days. These associations remained 3-4 days later, but generally not 7 days later. There was no association between any virus positivity and changes in electronically recorded PEFR and FEV1. CONCLUSIONS: In a longitudinal study of children with moderate asthma, the presence of rhinovirus in nasal wash, but not in exhaled breath, was associated with worsening of several indices of respiratory symptoms for up to a week.
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J ALLERGY CLIN IMMUNOL VOLUME 133, NUMBER 2
Effect Of Prenatal Exposure To Indoor PM2.5 and Environmental Tobacco Smoke Affecting Lower Respiratory Tract Infection Was Modified By ROS Genes: Cocoa Study Dr. Song I. Yang, MD1, Dr. Eun Lee, MD1, Dr. Young Ho Jung, MD1, Kil-Yong Choi2, Mi-Jin Kang, MS2, Ho-Sung Yu2, Cheol Min Lee3, Prof. Youn Ho Shin, MD, PhD4, Prof. Kangmo Ahn, MD, PhD5, Prof. Kyung Won Kim, MD, PhD6, Prof. Soo-Jong Hong, MD, PhD1 Cocoa study Group7; 1Childhood Asthma Atopy Center, Department of Pediatrics, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea, 2Asan Institute for Life Sciences, University of Ulsan College of Medicine, Seoul, South Korea, 3Institute of Environmental and Industrial Medicine, Hanyang University, Seoul, South Korea, 4 Department of Pediatrics, CHA Medical Center, CHA University College of Medicine, Seoul, South Korea, 5Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea, 6Department of Pediatrics, Yonsei University College of Medicine, Seoul, South Korea, 7COCOA study group. RATIONALE: Prenatal air pollution exposure associates with impaired fetal growth, decreased lung function, respiratory morbidity and mortality. We aimed to investigate the influence of prenatal indoor fine particulate matter (PM2.5) and environmental tobacco smoke (ETS) exposure and genetic polymorphisms on the susceptibility to respiratory tract infection (RTI) in infancy. METHODS: Demographic, environmental factors and diagnosis of RTI in the first year of life were evaluated in 231 infants from a birth cohort of general population in Korea between 2007 and 2011. Indoor PM2.5 was measured during the third trimester and mothers were asked about ETS exposure during pregnancy. Nuclear factor (erythroid-derived 2)-like 2 (NRF2, rs6726395), glutathione S-transferase pi 1 (GSTP1, rs1695) and glutathione S-transferase mu 1 (GSTM1, copy number variation) genotyping was performed. RESULTS: Prenatal indoor PM2.5 or ETS exposure increased the risk of bronchiolitis and lower RTI (LRTI), but not upper RTI (URTI), in infancy. They also synergistically increased susceptibility to bronchiolitis and LRTI (aOR 5.20; 95% CI 1.10-24.61, aOR 5.18; 95% CI 1.40-19.24, respectively), but not URTI. This synergistic effect was increased by Nrf2 GG (aOR 10.23; 95% CI 1.02-102.57, aOR 14.01; 95% CI 1.42-138.02, respectively) and GSTM1 null genotypes (aOR 9.45; 95% CI 1.03-86.78, aOR 5.70; 95% CI 1.02-31.95, respectively). CONCLUSIONS: Indoor PM2.5 and ETS exposure during the prenatal period synergistically increased susceptibility to bronchiolitis and LRTI, but not URTI. This effect was modified by polymorphisms in reactive oxygen species-related genes.
984
IgE Anti-Respiratory Syncytial Virus Antibodies In Older Asthmatic Children Mira Mandal1, Dr. Rauno O. Joks, MD, FAAAAI2, Dr. Kevin Norowitz, MD3, Dr. Diana Weaver, MD4, Dr. Helen G. Durkin, PhD5, Dr. Martin H. Bluth, MD, PhD6, Dr. Stephan Kohlhoff, MD5, Dr. Tamar A. Smith-Norowitz, PhD7; 1SUNY Downstate Medical Center, 2 SUNY-HSC, Brooklyn, NY, 3SUNY Downstate Medical Center, Center for Allergy and Asthma Research, Brooklyn, NY, 4SUNY Downstate Med. Ctr, Brooklyn, NY, 5Center for Allergy and Asthma Research, State University of New York Downstate Medical Center, Brooklyn, NY, 6 Wayne State University Medicine, Detroit, MI, 7Center for Allergy and Asthma Research at SUNY Downstate, Brooklyn, NY. RATIONALE: Respiratory syncytial virus (RSV) causes severe lower respiratory tract disease in infant and young children, and is a serious public health concern, as is the increase in pediatric asthma in the United States. However, it remains unknown if IgE and RSVantibodies (Abs) play a role in development of asthma. METHODS: Total serum IgE, and IgE and IgG anti-RSV Ab responses were studied in older asthmatic compared with non asthmatic children (M/ F, mean age: 14) (N5 30, N543, respectively) (UniCAP total IgE
Fluoroenzymeimmunoassay (IU/mL), enzyme-linked immunosorbent assay (U/mL; OD value). RESULTS: Total serum IgE levels were increased 3-fold in asthmatic compared with non asthmatic children (323 + 217, 107 + 116, P5 0.0003). Further, IgE anti-RSV Ab levels were 1.5-fold higher in asthmatic compared with nonasthmatic children (P 5 0.01); IgG anti-RSVAb levels were slightly increased in the asthmatic children (112 + 32, 81+ 59, P50.003). Interestingly, values of IgE anti-RSV Abs positively correlated with IgG anti-RSV Abs. CONCLUSIONS: The presence of IgE anti-RSV abs in older children further lends support to an important role of RSV infection in asthma.
985
Asthma Symptoms and Rhinovirus In A Longitudinal Children's Cohort Dr. Euan R. Tovey, PhD1,2, Dr. S. Stelzer-Braid, PhD3,4, Dr. B. G. Toelle, PhD1, Ms. C. M. Willenborg, BSc3, Dr. H. K. Reddel, PhD, MBBS1,2, Ms. F. L. Garden, MPH1,2, Prof. A. Jaffe, PhD, MD4,5, Ms. R. Strachan5, Dr. B. G. Oliver, PhD1,2, Dr. Y. C. Belessis, MBBS4,5, Prof. G. B. Marks, PhD, MD1,2, Prof. W. D. Rawlinson, PhD, MD3,4; 1 Woolcock Institute of Medical Research, Sydney, Australia, 2University of Sydney, Australia, 3Virology Division, SEALS Microbiology, Prince of Wales Hospital, Sydney, Australia, 4University of NSW, Australia, 5 Sydney Children’s Hospital, Randwick Australia. RATIONALE: Emergency presentations for asthma are strongly associated with rhinovirus (HRV) infections in children. However, community viral infections are common and often mild or asymptomatic, and the role of viruses in changing daily symptoms is less clear. We hypothesized such infections would be associated with increased symptoms. METHODS: 67 children, aged 5-12, with moderately severe asthma, selfcollected nasal-wash and exhaled breath samples and recorded their recent asthma and cold symptoms and current lung function, twice per week for 10 weeks. The presence of 8 viruses, including rhinovirus (HRV), was analysed by PCR. A mixed model, to account for repeated measures, was used to determine the current and delayed impact of viruses on symptoms and lung function. RESULTS: 25.5% of nasal samples and 11.5% of breath samples were positive for HRV; only 1.8% were positive for other viruses. The presence of HRV in nasal wash, but not in breath, was associated with the presence of symptoms; adjusted odds ratios: cough 2.53, (95%CI 1.62-3.94), wheeze 3.05, (95%CI 1.89 to 4.93), self-reported febrile 2.07, (95%CI 1.17 to 3.64) and coryzal symptoms 1.95, (95%CI 1.14 to 3.32) over the previous 3-4 days. These associations remained 3-4 days later, but generally not 7 days later. There was no association between any virus positivity and changes in electronically recorded PEFR and FEV1. CONCLUSIONS: In a longitudinal study of children with moderate asthma, the presence of rhinovirus in nasal wash, but not in exhaled breath, was associated with worsening of several indices of respiratory symptoms for up to a week.
TUESDAY
983