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Immunochemistry
306 tigious group of authors has been assembled to review current topics such as chromatography, capillary electrophoresis as well as optimization in the field of separation science. The use of capillary electrophoresis (CE) grows rapidly for the analysis of biological molecules such as proteins, carboh y ~ t e s , etc, Two chapters concern this technique. The. top.it el' the first one is related to the potential use of CE m the analysis of human serum and plasma proteins. The applications of various modes of CE are discussed. The knowledge gained from the separation of blood proteins will certainly be usefid for other protein'sepatations. The aims of the second chapter arc to design app~aches and concepts that are most useful in the separation of carbohydrates by CE. Emphasis is put on the use of CE in the study of complex saecharides. The dynamic behavior of carbohydrates in the electrophoretic process ts outlined. The field of chromatography is very well addressed in three excellent reviews on gas chromatography (GC), supereritical fluid chromatography (SFC) and liquid chromatography (LC). A chapter reviews the spectrometric analysis by gas chromatography/matrix isolation/infrared spectrometry (GC/MI/IR). It focuses on IR detection. Information related to the characteristics of MI/IR spectra of natural products, after their purification by GC, is discussed. A separate chapter describes the state-of-art in environmental applications of SFC. This technique provides the means to couple a fast, versatile separation process to a wide variety of detectors. In the next chapter, data on retention of homologous series of simple organic ions using LC are analyzed. The author stddies the behavior of the retention under different conditions of temperature and mobile phase and using a broad variety of stationary phases (reverse-phase, ion-exchange phase). Two chapters are dedicated to the separation science from a theorical point of view The first one is a review of the recent literature concerning various models of overlap in chromatography by statistical means and computer simulations. Several theories in one-dimensional (chromatography, clectrophoresis) as weft as in multi-dimensional separations (two-dimensional thin layer chromatography) are described. The crucial point of the second chapter is the emergence of a concept able to fit the uncertainties of quantitative analysis. Possible application of this c~neept to develop an optimization system in separation selene© ~salso discussed. In summary, this volume is a useful and attractive book for scientists actively engaged in research and development in the field of separation science.
The first part of the book (270 pages) pertains to basic aspects of immunoglobulins and related proteins. It contains sections on structure, function, phylogeny of immunoglobulins, T-cell receptors, major histoeompatibility complex class II molecules, Fc receptors and lectins. The focus of this part includes not only monoclonal antibodies but deals also with recombinant antibodies and immunoglobulins as enzymes (abzymes). However, this section suffers from different kind of problems, whole reviews have now been devoted to some cf the subject areas of this sect,on, and the treatment of some subjects is superficial. In pa~icular, the overview of bispecific and chimeric antibodies is relegated to 12 lines. Granted, space limitations in a general textbook of this type do not allow comprehensive treatmen~ of the subject, but a description of this powerful and relatively recent application of immunochemistry is lacking. Part two of the book (304 pages), dealing with antigens and haptens, is interesting reading because of the stature of the contributors and the variety of subjects covered. Indeed, this part describes numerous relevant antigens such as: peptides, proteins, polysaccharides, nucleic acids, pharmacological substances, tumor-associated antigens, bacterial, viral and parasite antigens, allergens. A chapter on immunochemistry of vaccines is included. The third part of the book (392) deals with fundamentals of antigen-antibody interactions and some in vice or in vitro consequences and applicativns of these interactions, namely, circulating immune complexes, autoantigens, immunotargeting, precipitation and agglutination, ELISA, determination of antibody affinity, immunomicroscopy methods, flow cytometry, complement, immunoblotting, biosensor techniques. As a chapter describing the lectins is added to the first part of the book, the addition of a chapter in part three on the use of biotin-avidin interaction in immunochemistry would have been interesting for the readership. Part four (80 pages) is devoted to the regulation of the immune response and contains two chapters discussing the structure and the role of cytokines and lymphokines, one chapter dealing with immt, nosuppression, and one short chapter reviewing the molecular and cellular mechanisms that result in affinity maturation. Each chapter is concise and well illustrated. The great value of Immunochemistry is that it presents in a single volume an authoritative review of current information on all aspects of the immunochemical interactions. It provides an excellent source of reference that should prove useful to rescarch workers and graduate students.
A Namane JL Guesdon
lmmunochemistry, edited by CJ van Oss, MHV van Regenmortel, Marcel Dekker lnc, 1994, $195, 1069 p The recer.t book lmmunochemistry, written by 60 international experts, offers comprehensive, up-to-date coverage of the chemical and physicochemieal aspects of immunological interactions. It covers the whole area of im~unochemistry including the basic principles and methods for antibody preparation and antibody analysis as well as in vice and in vitro applications of antigen-antibody interactions.
Crystal Structure Analysis for Chemists and Biologists, JP Glusker, VCH Publishers lnc, 1994, DM 120,00, 854 p The knowledge of the three-dimensional structure of biologically in:,portant molecules, and in particular macromolecules, has become an indispensable part of most biological studies. Of the two principal methods of structure analysis used today, NMR and X-ray crystallography, the latter ig the older and has therefore produced the largest number of structures.
The first part of the book (270 pages) pertains to basic aspects of immunoglobulins and related proteins. It contains sections on structure, function, phylogeny of immunoglobulins, T-cell receptors, major histoeompatibility complex class II molecules, Fc receptors and lectins. The focus of this part includes not only monoclonal antibodies but deals also with recombinant antibodies and immunoglobulins as enzymes (abzymes). However, this section suffers from different kind of problems, whole reviews have now been devoted to some cf the subject areas of this sect,on, and the treatment of some subjects is superficial. In pa~icular, the overview of bispecific and chimeric antibodies is relegated to 12 lines. Granted, space limitations in a general textbook of this type do not allow comprehensive treatmen~ of the subject, but a description of this powerful and relatively recent application of immunochemistry is lacking. Part two of the book (304 pages), dealing with antigens and haptens, is interesting reading because of the stature of the contributors and the variety of subjects covered. Indeed, this part describes numerous relevant antigens such as: peptides, proteins, polysaccharides, nucleic acids, pharmacological substances, tumor-associated antigens, bacterial, viral and parasite antigens, allergens. A chapter on immunochemistry of vaccines is included. The third part of the book (392) deals with fundamentals of antigen-antibody interactions and some in vice or in vitro consequences and applicativns of these interactions, namely, circulating immune complexes, autoantigens, immunotargeting, precipitation and agglutination, ELISA, determination of antibody affinity, immunomicroscopy methods, flow cytometry, complement, immunoblotting, biosensor techniques. As a chapter describing the lectins is added to the first part of the book, the addition of a chapter in part three on the use of biotin-avidin interaction in immunochemistry would have been interesting for the readership. Part four (80 pages) is devoted to the regulation of the immune response and contains two chapters discussing the structure and the role of cytokines and lymphokines, one chapter dealing with immt, nosuppression, and one short chapter reviewing the molecular and cellular mechanisms that result in affinity maturation. Each chapter is concise and well illustrated. The great value of Immunochemistry is that it presents in a single volume an authoritative review of current information on all aspects of the immunochemical interactions. It provides an excellent source of reference that should prove useful to rescarch workers and graduate students.
A Namane JL Guesdon
lmmunochemistry, edited by CJ van Oss, MHV van Regenmortel, Marcel Dekker lnc, 1994, $195, 1069 p The recer.t book lmmunochemistry, written by 60 international experts, offers comprehensive, up-to-date coverage of the chemical and physicochemieal aspects of immunological interactions. It covers the whole area of im~unochemistry including the basic principles and methods for antibody preparation and antibody analysis as well as in vice and in vitro applications of antigen-antibody interactions.
Crystal Structure Analysis for Chemists and Biologists, JP Glusker, VCH Publishers lnc, 1994, DM 120,00, 854 p The knowledge of the three-dimensional structure of biologically in:,portant molecules, and in particular macromolecules, has become an indispensable part of most biological studies. Of the two principal methods of structure analysis used today, NMR and X-ray crystallography, the latter ig the older and has therefore produced the largest number of structures.