Immunocytochemical study on human-leumorphin in the spinal cord of the Japanese monkey (macaca fuscata )

S93 THE I ~ U N O C Y T O C H E M I C A L

LOCALIZATION

LEU 8 IN RELATION TO THE MONOAMINE SADATSUGU MURAKAHI*,

HITOSHI OKAMURA*,

YANAIHARA 2, AND YASUHIKO Department Shizuoka

F~IIO KAWAKAMI *I , NOBORU

IBATA

of Anatomy*and

sity of Medicine,

OF MET-ENKEPHALIN-ARG6-GLY 7-

NEURON SYSTEM IN RAT BP~INSTEM

Psychiatry I, Kyoto Prefectural

Kamikyo-ku,

College of Pharmacy I, Shizuoka,

We examined

the distribution

(Iiet-Enk-8)and catecholamine

Japan

of Met- Enkephalin-Arg6-Gly7-Leu 8

in the rat brainstem.

combined method of PAP immunocytochemistry fluorescence to detect

histochemistry

of Met-Enk-8

The immunoreactive

neuronal

w=re widely distributed

in the brainstem,

interpedunclar

midbrain

solitary

nucleus,

tract,

tractus

nerve.

IM~NOCYTOCHEMICAL KENJI UDA*, Medicine,

of Anatomy,

Kamikyo-ku,

spinalis and

in A I, A 2, and A 5

groups.

IN THE SPINAL CORD

NOBORU YANAIHARA 1 and YASUHIKO

Kyoto Prefectural

University

IBATA

of

Kyoto 602, Japan.

Shizuoka College of Pharmacy I, Shizuoka. Human-leumorphin

of the

(MACACA FUSCATA)

HITOSHI O K A ~ R A * ,

2nd Department

nucleus

in the

containing Met-Enk-8

STUDY ON HUMAN-LE~.IORPHIN

OF THE JAPANESE MONKEY

in

of Met-Enk-8

particularly

were detected

neuronal

acid in order

and catecholamines perikarya

nucleus,and

The neurons

simultaneously

areas of catecholamine

and glyoxylic

central grey,nucleus

lateral reticular

trigeminal

catecholamine

We applied a

in the same tissue sections

the colocalization

one neuron.

Univer-

Kyoto 602, Japan

immunoreactive

spinal cord of Japanese monkeys.

Japan.

elements were revealed

in the

For the immunocytochemical

procedure,

transverse

and parasagittal

thickness)

were made,

and they were stained by immunocytochemistry

of the PAP method.

Immunoreactive

densely distributed

in laminae

distribution laminae

with moderate

III-VII

colchicine

neuronal

the immunoreactive laminae.

(Rexed)

I, and II, and their

or slight density was also observed levels

at all segmental

In laminae

in

(C-I - Co-l).

perikarya were detected

grey substance

pretreatment.

(40 ~m

fibers and terminals were

and X at all segmental

Immunoreactive and intermediate

frozen sections

in the dorsal

levels after

I, and II, the population

perikarya was larger than in any other

of