Immunoelectrophoretic determination of brain-specific antigens in bulk-prepared neuronal and glial cells

Immunoelectrophoretic determination of brain-specific antigens in bulk-prepared neuronal and glial cells

335 The present study was supported by a grant from the Swedish Medical Research Council (No. B76-12X-00164-12C). REFERENCES 1 Bignami, A., Eng, L. F...

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335 The present study was supported by a grant from the Swedish Medical Research Council (No. B76-12X-00164-12C). REFERENCES 1 Bignami, A., Eng, L. F., Dahl, D. and Uyexia, C. T., Localization of the glial flbriUary acidic protein in astrocytes by immunofluorescence, Brain Research, 43 (1972) 429435. 2 Blomstrand, C. and Hamberger, A., Protein turnover in cell-enriched fractions from rabbit brain, J. Neurochem., 16 (1959) 1401-1407. 3 BIomstrand, C. and Hambcrger, A., Amino acid incorporation in vitro in proteins of neuronal and glial cell enriched fractions, J. Neurochem., 17 (1970) 1187-1195. 4 Bock, E., Identification and characterization of water-soluble rat brain antigens. I. Brain and species specificity, J. Neurochem., 19 (1972) 1731-1736. 5 Bock, E. and Dissing, J., Demonstration of enolase activity connected to the brain specific protein 14-3-2, Scand. J. Immunol., 4, Suppl. 2 0975) 31-36. 6 Bock, E., Jorgensen, O. S., Dittman, L. and Eng, L. F., Determination of brain-specific antigens in short term cultivated rat astroglial cells and in rat synaptosomes, J. Neurochem., 25 (1975) 867-870. 7 Cotman, C. W. and Matthews, D. A., Synaptic plasma membranes from rat brain synaptosomes: isolation and partial characterization, Biochim. biophys, acta (Amst.), 249 (1971) 380-394. 8 Cotman, C., Herschman, H. and Taylor, D., Subcellular fractionation of cultured glial cells, J. Neurobiol., 2 (1971) 169-180. 9 Eng, L. F., Vanderhaeghen, J. J., Bignami, A. and Gerstl, B., An acidic protein isolated from fibrous astrocytes, Brain Research, 28 (1971) 351-354. 10 Gray, E. G. and Whittaker, V. P., The isolation of nerve endings from brain: an electron microscopic study of cell fragments derived by homogenization and centrifugation, J. Anat. (Lond.), 96 (1962) 79-87. 11 Haglid, K. G. and Stavrou, D., Water-soluble and pentanol-extractable proteins in human brain normal tissue and human main tumours, with special reference to S-100 protein, J. Neurochem., 20 (1973) 1523-1532. 12 Hamberger, A. and Svennerholm, L., Composition of gangliosidcs and phospholipids of neuronal and glial cell enriched fractions, J. Neurochem., 18 (1971) 1821-1829. 13 Hamberger, A., Hansson, H.-A. and Sellstr~m, A., A morphological study of bulk-prepared neuronal perikarya and glial cells, Exp. Cell Res., 92 (1975) 1-10. 14 H•nn• F. A.• The structure and functi•n •f glia• ce•• membran•s•Abstr. 5th •SN Meeting•Barce••na• 1975, p. 33. 15 Jorgensen, O. S. and Bock, E., Brain specific synaptosomal membrane proteins demonstrated by crossed immunoelectrophoresis, J. Neurochem., 23 (1974) 879-880. 16 Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J., Protein measurement with the Folin phenol reagent, J. biol. Chem., 193 (1951) 265. 17 Moore, B. W. and McGregor, D., Chromatographic and electrophoretic fractionation of soluble proteins of brain and liver, J. biol. Chem., 240 (1965) 1647-1653. 18 Morgan, I. G., The isolation of synaptosomal subfractions, A bstr. INS Satelline Symp., G6ttingen, 1975. 19 Poduslo, S. E. and Norton, W. T., The bulk separation of neuroglia and neuronal perikarya. In N.Marks and R. Rodnight (Eds.), Research Methods in Neurochemistry, Vol. 1, Plenum Press, New York, 1972, pp. 19-32. 20 Rose, S. P. R., Neurons and glia: separation techniques and biochemical interrelationships. In A. Lajtha (Ed.), Handbook of Neurochemistry, Vol. 2, Plenum Press, New York, 1969, pp. 183-193. 21 Sellinger, O. Z. and Azcurra, J. M., Bulk separation of neuronal cell bodies and glial cells in the absence of added digestive enzymes. In N. Marks and R. Rodnight (Eds.), Research Methods in Neurachemistry, Vol. 2, Plenum Press, New York, 1974, pp. 3-38. 22 Warecka, K. and Bauer, H., Studies on 'brain-specific' proteins in aqueous extracts of brain tissue, J. Neurochem., 14 (1967) 783-787. 23 Yanagihara, T. and Hamberger, A., Effect of diphenylhydantoinon protein metabolism in neuron and neuroglial fractions of central nervous tissue, Exp. Neurol., 32 (1971) 152-162.