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Immunoglobulins in mouse uterus and embryo during the oestrous cycle and the first half of pregnancy
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IMMUNOGLOBULINS IN MOUSE UTERUS AND EMBRYO DURING THE OESTROUS CYCLE AND THE FIRST HALF OF PREGNANCY. Flore Rachman, Viviane Casimiri , Alexandre Psychoyos , Olivier Bernard. Unit~ de Recherche d'H~patologie Infantile, INSERM U 56, * Laboratoire de Physiologic de la Reproduction, ER 203 CNRS, HSpital de Bic@tre, F-94270 Bic~tre, France. The distribution of IgG, IgA and IgM was studied by an immunoperoxidase technique during the oestrous cycle and the first half of pregnancy in the mouse uterus and embryo. At pro-oestrus, IgA-plasma cells and free IgG invaded the stroma, IgA and IgG were observed in 40 % and i0 % of glandular lumina, respectively, and in the uterine lumen. IgA and IgG decreased at oestrus and were low at the other stages. IgM were mostly found in blood vessels. During pregnancy, from day 3 to i0, similar invasion of the stroma with IgA-plasma cells and free IgG was observed and resulted in persisting presence of IgA in most glands, and of IgG and IgA in the lumen. Luminal epithelial cells contained apical granules of IgG at the implantation site on day 5 and apical granules of IgA in the interimplantation area on days 4.5 to 6.5. In the implanted embryo, IgG, some IgA and rare IgM were visible in the trophoblast, yolk cavity and endoderm. These results (i) further support ovarian hormonal regulation concept of immunoglobulin secretion in the rodent uterus during the oestrous cycle, (2) show that all three classes of immunoglobulins are transmitted to the implanted mouse embryo, (3) suggest that modification of endo or exocytosis processes with respect to immunoglobulins, appear in the luminal epithelium of the implantation site, (4) indicate that the hormonal condition of pregnancy and/or the presence of the embryo may be responsible for persistent immunoglobulin secretion.
THE P~CULIARITIES OF THE CELLULAR AND HUMORAL I~IJNITY IN COWS AT NORMAL AND PATHOLOGICALDELIVERY. Falentin Yablonsk~ and Fas~l Prygara.Chair of Obstetrics and Artificial insemenation, Kamenets-Podolsky Agricultural institute, KhmelnitskyRegion, 28I 900, USSR. The tests were carried on the black-and-white breed cows before I-3 days of the delivery, in the delivery-day, on the 3-5, 8-I0, I8-20, 28-30-th days. The group of nonpregnant cows was served as acontrol. The relative and absolute content of T-, B- an "0" - lymphocytes was determined in the blood samples. Results: before I-3 days of the delivery the amount of T- and B- lymphocytes in cows of the last month of pregnancy was lower than in nonpregnant one. The sure increase in number of T-, Blymphocytes takes place on the 3-dS-th day after the delivery, but their quantity esentially differs from the quantity of the nonpregnant ones on the 8-IO-th day. At the pathological delivery there were marked the lower amoun~ of T- and ]3- lymphocytes and the slower renewal of their quantity in the post-natal period in compared with the normal one. The obtained data illustrate the considerable shift in the immune system of cows in the period of delivery and in the postnatal period, as well as the lowering of the cellular and humoral immunity at the pathological delivery.
IMMUNOGLOBULINS IN MOUSE UTERUS AND EMBRYO DURING THE OESTROUS CYCLE AND THE FIRST HALF OF PREGNANCY. Flore Rachman, Viviane Casimiri , Alexandre Psychoyos , Olivier Bernard. Unit~ de Recherche d'H~patologie Infantile, INSERM U 56, * Laboratoire de Physiologic de la Reproduction, ER 203 CNRS, HSpital de Bic@tre, F-94270 Bic~tre, France. The distribution of IgG, IgA and IgM was studied by an immunoperoxidase technique during the oestrous cycle and the first half of pregnancy in the mouse uterus and embryo. At pro-oestrus, IgA-plasma cells and free IgG invaded the stroma, IgA and IgG were observed in 40 % and i0 % of glandular lumina, respectively, and in the uterine lumen. IgA and IgG decreased at oestrus and were low at the other stages. IgM were mostly found in blood vessels. During pregnancy, from day 3 to i0, similar invasion of the stroma with IgA-plasma cells and free IgG was observed and resulted in persisting presence of IgA in most glands, and of IgG and IgA in the lumen. Luminal epithelial cells contained apical granules of IgG at the implantation site on day 5 and apical granules of IgA in the interimplantation area on days 4.5 to 6.5. In the implanted embryo, IgG, some IgA and rare IgM were visible in the trophoblast, yolk cavity and endoderm. These results (i) further support ovarian hormonal regulation concept of immunoglobulin secretion in the rodent uterus during the oestrous cycle, (2) show that all three classes of immunoglobulins are transmitted to the implanted mouse embryo, (3) suggest that modification of endo or exocytosis processes with respect to immunoglobulins, appear in the luminal epithelium of the implantation site, (4) indicate that the hormonal condition of pregnancy and/or the presence of the embryo may be responsible for persistent immunoglobulin secretion.
THE P~CULIARITIES OF THE CELLULAR AND HUMORAL I~IJNITY IN COWS AT NORMAL AND PATHOLOGICALDELIVERY. Falentin Yablonsk~ and Fas~l Prygara.Chair of Obstetrics and Artificial insemenation, Kamenets-Podolsky Agricultural institute, KhmelnitskyRegion, 28I 900, USSR. The tests were carried on the black-and-white breed cows before I-3 days of the delivery, in the delivery-day, on the 3-5, 8-I0, I8-20, 28-30-th days. The group of nonpregnant cows was served as acontrol. The relative and absolute content of T-, B- an "0" - lymphocytes was determined in the blood samples. Results: before I-3 days of the delivery the amount of T- and B- lymphocytes in cows of the last month of pregnancy was lower than in nonpregnant one. The sure increase in number of T-, Blymphocytes takes place on the 3-dS-th day after the delivery, but their quantity esentially differs from the quantity of the nonpregnant ones on the 8-IO-th day. At the pathological delivery there were marked the lower amoun~ of T- and ]3- lymphocytes and the slower renewal of their quantity in the post-natal period in compared with the normal one. The obtained data illustrate the considerable shift in the immune system of cows in the period of delivery and in the postnatal period, as well as the lowering of the cellular and humoral immunity at the pathological delivery.