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Immunological abnormalities in glomerulonephritides
ABSTKACTS OF ANNUAL MEFTIYG
I979
3nc;
I n the human situation, markers to differentiate T helper and T suppressor (Ts) lymphocytes have not hecn available until recently. Evidence now exists that human T lymphocytes bearing receptors for the Fc portion of IgG (Tg)are capable of suppressing some immune responses; while T lymphocytes with IgM Fc receptors (Tp)may help the response. In diseases like hypogamniaglobulineniia, in which excessive Ts activity can occur. an increased number of Ty cells has becn observed in the peripheral blood. On the other hand. systemic lupus erythematosus. and autoimmune disease with evidence of impaired Ts function, has been shown to have reduced numbers oI'T;, cells. Chronic ITP has many clinical and immunological features of autoimmune disease. Furthermore there is some evidence that both humoral and cellular immunity in this disorder are abnormal. To quantitate Ts activity in chronic ITP, T;, levels were determined in the peripheral hlood of 13 patients with this disorder. Nine o u t 01' I3 patients had unequivocally low levels ofT; cells. However. no correlation was found between the percentapt. of T;, cells and the clinical state. platelet count or current therapy. These results were consistent with the demonstrntion of loss ofTs activity in chronic ITP using in i'itro IgG synthesis assay. Diseases known to he arwciated w i t h elecatcd Ts activity. e.g. hypogammaglobulinemia or chronic GVH disease were used a s posit(\i' Lontrola. Whether the loss of Ts activity and function in this group of patients irepi-L.;i.iirh a primary defect in immunoregulation or is a secondary phenomenon due to receptor blockage by immunc ctjmplexes or redistribution of Ts cells to sites other than blood. remains to he established.
IMMUNOLOGICAL ABNORMALITIES IN GLOMERULONEPHRITIDES
R. S. WALLS,G . CARNEY. C. R . P. CEOKCih. J. P A Y U P . R. C. NFWLANU & J . R. LAWKIPKI; c
IN VlVO ANTINUCLEAR ANTIBODIES (ANA) IN RENAL BIOPSIES
J . V. WELLS,A. M. VAN DWENTEK. J . Wbne & L. S. IBELS
Kolling fvi.stitutr of Mrtlic,ul Re.rcwrch. Sutton Rhrurnnti.~n7Rrsmrc.li Lohorutory nnd Rerial b'nit, Royul Nortlr Show Hospirrri of' $ 1 clnc,i.
I n rivo untinuclear antibody (ANA) was observed by direct immunofluorescence microscopy in ccll nuclei in 16 renal biopsies from 15 patients (V,, renal biopsies). The 15 patients included I 3 with systemic lupub cI-ythematosus (SLE) and one each with mixed connective tissue disease and progressive systemic sclerosis. The in vivo biopsy A N A pattern was speckled in 12 patients. and thc A N A class was IgC i n 15, IgA in 4. and IgM in 7. Complement was not detected in the cells. The glomeruli showed granular mesangial or capillary wall deposition in 14cases for IgG, 9 !or IgA. 15 for IgM. and 1.2 for complement. Light microscopy showed on11 light or
I979
3nc;
I n the human situation, markers to differentiate T helper and T suppressor (Ts) lymphocytes have not hecn available until recently. Evidence now exists that human T lymphocytes bearing receptors for the Fc portion of IgG (Tg)are capable of suppressing some immune responses; while T lymphocytes with IgM Fc receptors (Tp)may help the response. In diseases like hypogamniaglobulineniia, in which excessive Ts activity can occur. an increased number of Ty cells has becn observed in the peripheral blood. On the other hand. systemic lupus erythematosus. and autoimmune disease with evidence of impaired Ts function, has been shown to have reduced numbers oI'T;, cells. Chronic ITP has many clinical and immunological features of autoimmune disease. Furthermore there is some evidence that both humoral and cellular immunity in this disorder are abnormal. To quantitate Ts activity in chronic ITP, T;, levels were determined in the peripheral hlood of 13 patients with this disorder. Nine o u t 01' I3 patients had unequivocally low levels ofT; cells. However. no correlation was found between the percentapt. of T;, cells and the clinical state. platelet count or current therapy. These results were consistent with the demonstrntion of loss ofTs activity in chronic ITP using in i'itro IgG synthesis assay. Diseases known to he arwciated w i t h elecatcd Ts activity. e.g. hypogammaglobulinemia or chronic GVH disease were used a s posit(\i' Lontrola. Whether the loss of Ts activity and function in this group of patients irepi-L.;i.iirh a primary defect in immunoregulation or is a secondary phenomenon due to receptor blockage by immunc ctjmplexes or redistribution of Ts cells to sites other than blood. remains to he established.
IMMUNOLOGICAL ABNORMALITIES IN GLOMERULONEPHRITIDES
R. S. WALLS,G . CARNEY. C. R . P. CEOKCih. J. P A Y U P . R. C. NFWLANU & J . R. LAWKIPKI; c
IN VlVO ANTINUCLEAR ANTIBODIES (ANA) IN RENAL BIOPSIES
J . V. WELLS,A. M. VAN DWENTEK. J . Wbne & L. S. IBELS
Kolling fvi.stitutr of Mrtlic,ul Re.rcwrch. Sutton Rhrurnnti.~n7Rrsmrc.li Lohorutory nnd Rerial b'nit, Royul Nortlr Show Hospirrri of' $ 1 clnc,i.
I n rivo untinuclear antibody (ANA) was observed by direct immunofluorescence microscopy in ccll nuclei in 16 renal biopsies from 15 patients (V,, renal biopsies). The 15 patients included I 3 with systemic lupub cI-ythematosus (SLE) and one each with mixed connective tissue disease and progressive systemic sclerosis. The in vivo biopsy A N A pattern was speckled in 12 patients. and thc A N A class was IgC i n 15, IgA in 4. and IgM in 7. Complement was not detected in the cells. The glomeruli showed granular mesangial or capillary wall deposition in 14cases for IgG, 9 !or IgA. 15 for IgM. and 1.2 for complement. Light microscopy showed on11 light or