Immunological techniques

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Immunological techniques Developments in T-cell assays Elements Active in T-tymphocytes. J Biol Chem 1993, 268:21946-21954. Viale AC, Freitas AA, Coutinho A: Regulation of V-Hgene Expression is a Lineage-specific Developmental Marker. Eur J Immuno11994, 24:1353-1358. Victor KD, Vu K, Feeney AJ: Limited Functional Diversity in Kappa Lighl Chains - Junctional Sequences from Cd43(+)B220(+) Early B Cell Progenitors Resemble those from Peripheral B Cells. / Immunol 1994, 152:3467-3475. Weissman IL: Developmental Switches in the Immune System. Cell 1994, 76:207-218. West SC: The Processing of Recombination Intermediates - Mechanistic Insights from Studies of Bacterial Proteins. Cell 1994, 76:9-15. Wiest DL, Kearse KP, Shores EW, Singer A: Developmentally Regulated Expression of CD3 Components Independenl of Cionotypic T Cell Antigen Receplor Complexes on Immature Thymocytes. J Exp Meal 1994, 180:1375-1382.

Wilson A, Held W, Macdonald HR: Two Waves of Recombinase Gone Expre~ion in Developing Tbymocytes. J Exp Meal 1994, 179:1355-1360. Xia F, Amundsen SA, Nickoioff JA, Liber HL: Different Capacities for Recombination in Closely Related Human Lymphobiastoid Cell Lines with Different Mutational Responses to X-irradlation. Mol Cell Biol 1994, 14:5850-5857. Young F, Ardman B, Shinkai Y, Lansford R, Blackwell TK, Mendelsohn M, Rolink A, Melchers F, Ah FW: Influence of Immunogiobulin Heavy- and LightChain Expre~ion on B-Cell Differentiation. Genes Dev 1994, 8:1043-1057.

Differences between species in lymphocyte development

Distantly Related to Proboscipedia is Expressedin Lymphoid and Pancreatic Tissues. l Biol Chem 1994, 269:19968-19975.

Hengartner MO, Horvitz HR: Activation of C. Elegans Cell Death Protelo CED-9 by an Amlno-Acid Substitution in a Domain Conserved in Bd-2. Nature 1994, 369:318-320. Kumar S, Kinoshita M, Noda M, Copeland NG, Jenkins NA: Induction of Apoptosis by the Mouse Nedd2 Gene, Which Encodes a Protein Similar to the Product of the Caenorhabditis Elegans Cell Death Gene Ced-3 and the Mammalian IL-1 Beta-converting Enzyme. Genes Dev 1994, 8:1613-1626.

Harrison KA, Druey KM, Deguchi Y, Tuscano JM, Kehrl JH: A Novel Human Humeobox Gene

Immunological techniques Developments in T-cell assays Related review: Single T cell probes for antigen/MHC expressions(pp 258-262) Ahmed SA, Gogal RM, Walsh JE: A New Rapid and Simple Non-Radioactive Assay to Monitor and Determine the Proliferation of Lymphocytes - an Alternative to [h3]thymidine Incorporation Assay. J Immunol Methods 1994, 1 7 0 : 2 1 1 - 2 2 4 .

Blomberg K: Simultaneous Measurement of Natural Killer Cell Cytotuxicity Against Each of Three Different Target Cell Lines. J Immunol Methods 1994, 168:267-273. Bohlen H, Manzke O, Engert A, Hertel M, Hipplerahenburg R, Diehl V, Tesch H: Differentiation of Cytotoxicity Using Target Cells Labelled with Europmm and Samarium by Electroporation. J Immunol Methods 1994, 173:55-62. Brombacher F, Schafer T, Weissenstein U, Tschopp C, • Anderesen E, Burki K, 8aumann G: IL-2 Promoterdriven/acZ expreuion as a Monitoring Tool for IL-2 Expression in Primary T Cells of Transgenic Mice. Int Immuno11994, 6:189-197. [14]. Bromley L, Mccarthy SP, Slickland JE, Lewis CE, Mcgee JOD: Non-Isotopic in Situ Detection of Mma for Intedeukin-4 in Archival Human Tissue. J Immunol Methods 1994, 167:47-54. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prashar DC: • • Green Fluorescent Protein as a Marker for Gene Expre'Jsion. Science 1994, 263:802-805. [33]. Cox AL, Skipper J, Chen Y, Henderson RA, Darrow TL, • e Shabanowitz J, Engelhard VH, Hunt DF: Identification of a Peptide Recognized by Five Melanocna-specific Human Cytoloxic T cell Lines. Science 1994, 264:716-719. [24]. Daubener W, Wanagat N, Pilz K, Seghrouchni S, Fischer HG, Hadding U: A New, Simple, Bioassay for Human IFN-gamcna. J Immunol Methods 1994, 168:3947. Franke L, Porstmann 1-."A Highly Sensitive Non-radioactive Cytotoxicity Assay for Human Target Cells. J Immunol Methods 1994, 171:259-262. Friedl P, Noble PB, Zanker KS: Lymphocyte Locomotion in 3-Oimeosional Collagen Gels - Comparison of Three Quantitative Methods for Analysing Cell Trajectories. ] Immunol Methods 1993, 165:157-165. Galvez J, Cabrera L, Lajarin F, Garciapenarrubia P: Binding Units (Bu) and the Area Under Binding Isotherms (Aui) - New Indices of Effector-Targel Conjugation. J Immunol Methods 1994, 170:197-210. Hut KM, Sabapathy TK, eel AA, Chia TF: Generation of AIIo-reactive Cytoloxic T Lymphocytes by Particle Bombardment-mediated Gene Transfer. J Immunol Methods 1994, 171:147-155. James K, Milne I, Cunningham A, EllioK SF: The Effect of Alpha(2) Macrngiobulin in Commercial Cytokine Assays. J Immunol Methods 1994, 168:33-37. Karawajew L, Micheel B, Jung GR, Wolf H, 8ehrsing O: A Simple and Sensitive Method to Study Effects Mediated by Soluble Lymphokines as Demonstrated by the Interaction of CD4+ and COB+ Cell Subsets During T Cell Activation. J Immunol Methods 1994, 173:27-31. Kobayashi E, Kamada N, Enosawa S, Toyama N, Delriviere L, Goto S, Lord R, Stamatiou S, Miyata M: A

Technique for Complete Tbymectumy in Adult Rats. J Immunol Methods 1994, 171:33-36. Lichtenfels R, Biddison WE, Schulz H, Vogt AB, Martin R: CARE-LASS (calcein-release-assay), an Improved Fluorescence-based Test System to Measure Cylntoxic T Lymphocyte Activity. J Immunol Methods 1994, 172:227-239. Merkenschlager M, Fischer AG: In Vitro Construction of Graded Thymus Chimeras. J Immunol Methods 1994, 171:177-188. Miki I, Ishihara N, Otoshi M, Kase H: Simple Colorimetric Cell-Cell Adhesion Assay Using MTT-Stained Leukemia Cells. J Immunol Methods 1993, 164:255-261. Mourier G, Maillere B, Cotton J, Herve M, Leroy S, Leonetti M, Menez A: Boc-Cys(Npys)-OH (BCNP): an Appropriate Reagent for the Identification of T Cell Epltopes in Cystine And/or Cysteine.containing Proteins. J Immunol Methods 1994, 171:65-71. Munn LL, Glacken MW, Mcintyre BW, Zygourakis K: Analysis of Lymphocyte Aggregation Using Digital Image Analysis. J Immunol Methods 1993, 166:11-2,5. Negulescu PA, Shastri N, CAHALAN MD: Intraceliolar • Calcium Dependence of Gene Expression in Single T lymphocytes. Proc Natl Acad Sci U S A 1994, 91:2873-2877. [16]. Pryce G, Santos W, Male D: An Assay for the Analysis of Lymphocyte Migration Across Cerebral Endulhelium in Vitro. J Immunol Methods 1994, 167:55-63. Reece JC, Mcgregor DL, Geysen HM, Rodda SI: Scanning for T Helper Epllopes with Human PBMC Using Pools of Short Synthetic Peplides. J Immunol Methods 1994, 172:241-254. Sanderson S, Shastri N: LacZ Inducible Peptide/MHC e• Specific T-hybrids. Int Immuno11994, 6:369-376. [15]. Schlaak IF, Schmitt E, Huls C, Zumlouschenfelde KHM, Fleischer B: A Sensitive and Specific Bioassay for the Detection of Human Interleukin-10. ] Immunol Methods 1994, 168:49-54. Sellers JR, Cook S, Goldmacher VS: A Cyloloxiciiy Assay Utilizing a Fluorescent Dye thai Determines Accurale Surviving Fractions of Cells. J Immunol Methods 1994, 172:255-264. Shi YF, Kornovski 85, Savant R, Turley EA: A Rapid, Multiwell Colorimelric Assay for Chemntaxis. J Immunol Methods 1993, 164:149-154.

cDNA libraries

Related review: New methods to predict MHC-binding sequenceswith protein antigens (pp 263-269) Brusic '4, Rudy G, Harrison LC: Prediction of MHC • Binding Peptides Using Artificial Neural Networks. In Complex Systems: Mechanism of Adaptation. Edited by Stonier R], Yu XS. Amsterdam: lOS Press 1994, :253-260. 157]. Hammer J, 8one E, Gallazzi F, 8elunis C, Nagy Z, • Sinigaglia F: Precise Prediction of MHC Class II.peptide Interaction Based on Peptide Side Chain Scanning. J Exp Med 1994, 180:2353-2358. [46]. Nissim A, Hoogenboom HR, Tomlinson IM, Flynn G, Midgley C, Lane D, Winter G: Antibody

Fragments from a 'Single Pot' Phage Display Library as Immunocbemlcal Reagents. EMBO J 1994, 13:692-698. Parker KC, Bednarek MA, Coligan JE: Scheme for • Ranking Potential HLA-A2 Binding Peptides Based on Independent Binding of Individual Peplide Sidechains. J Immuoo11994, 152:163-175. [51]. Reay PA, Kantor RM, Davis MM: Use of Global Amino • Arid Replacements to Define the Requirements for MHC Binding and T Ceil Recognition of Moth Cytochrome C (93-103). J Immuno11994, 152:3946-3957. [52]. Ruppert J, Grey HM, Selte A, Kubo RT, Sidney J, Cells E: • Prominent Role of Secondary Anchor Residues in Peptide Binding to HLA-A2.1 Molecules. Cell 1993, 74:929-937. [47]. Smiley JA, Benkovic SJ: Selection of Catalytic Antibodies for a Biosynthetic Reaction from a Combinatorial CDNA Library by Complementation of an Auxotropbic Eschericbia Coil: Antibodies for Orotale Decarboxylation. Proc Natl Acad SCi U S A 1994, 91:8319-8323. Stern LJ, Brown IH, jardetzky TS, Gorga JC, Urban • • RG, Strominger JL, Wiley DC: Crystal Structure of the Human Class II MHC Protein HLA-DR1 Complexed wilh an Influenza Virus Peptide. Nature 1994, 368:215-221. [9].

Analysis of single cells Related review: Detection and isolation of rare cells (pp 270-273) 8aus E, Urbain I, Leo O, Andris F: Flow Cytometric Measurement of Calcium Influx in Murine T Cell Hybrids Using Flue-3 and an Organic-anion Transport Inhibitor. J Immunol Methods 1994, 173:41~17. Chang L, Gusewitch GA, Chrilton DSW, Folz JC, Lebeck LK, Nehlsencannarella SL: Rapid Flow Cytumetric Assay for the Assessment of Natural Killer Cell Activity. J Immunol Methods 1993, 166:45-54. Chen CH, Lin W, Shye S, Kibler R, Grenier K, Recktenwald • D, Terstappen LWMM: Aulumaled Enumeration of CD34 + Cells in Peripheral Blood and Bone Marrow. / Hematotherapy 1994, 3:3-13. [20]. Cook F, Eales LJ: Flow Cytometric Measurement of Non. Specific Fsterase Aclivity. J Immunol Methods 1994, 168:203-208. Ekong T, Kupek E, Hill A, Clark C, Davies A, Pinching A: Technical Influences on Immunophenotyplng by Flow Cytometry - the Effect of Time and Temperature of Storage on the Viability of Lymphocyte Subsets (Vol 164, Pg 263, 1993). J Immunol Methods 1993, 166:301. Garraud O, Perraul R, Gysin I, 8ehr C, Dubois P, Bonnemains B, Jouin H, Michel JC, Dasilva LP: Manipulating Blood T Cells and B Cells from Squirrel Monkeys: Some Technical Considerations. l Immunol Methods 1994, 173:165-173. Gross HJ, Verwer B, Houck D, Hoffman R, Recktenwald • D: Model Study Detecting Breast Cancer Cells in Peripheral Blood Mononoclear Cells at Frequencies as Low as 10 -7 . Proc Nail Acad Sci U S A 1995, 92:537-546. [3]. Irsch J, Irlenbusch S, Radl J, Burrows PD, Cooper MD, • Radbruch A: Switch Recombination in Normal IgA1

Immunological techniques PCR techniques B lymphocytes. Proc Natl Acad Sci U S A 1994, 91:1323-1327. [19]. Laviolette M, Bosse M, Rocheleau H, Lavigne S, Ferland C: Comparison of Two Modified Techniques for Purifying Blood Eosinophils. J Immunol Methods 1993, 165:253-261. Lillevang ST, Tort P, Nielsen B: A Method for Isoiating Granolocytes from Rabbit Blood Without Causing Activation. J Immunol Methods 1994, 169:137-138. Lohmeyer J, Friedrich J, Rosseau S, Pralle H, Seeger W: Multiparameter Flow CytomntrJc Analysis of Inflammatory Cdls Contained in Broochoalveolar Lavage Fluid. J Immunol Methods 1994, 172:59-70. Lu L, Woo J, Rao AS, Li YP, Watkins SC, Qian SG, Starzl TE, Demetris AJ, Thomson AW: Propagation of Dendritic Cell Progenitors from Normal Mouse Liver Using Granulocyte/macrophage Colony-stimulating Factor and Their Maturational Development in the Presence of Tylse-1 Collagen. J Exp Med 1994, 179:1823-1834. Lyons AB, Parish CR: Deten'olnation of Lymphocyte Division by Flow Cytometry. J Immunol Methods 1994, 171:131-137. Manz R, Assenmacher M, Pfl0ger E, Mihenyi S, Radbruch e• A: Analysis and Sorting of Live Cells According to Secreted Molecules, Relocated to a Cell Surface Affinity Matrix. P~oc Natl Acad Sci U S A 1995, in Press:. [22]. Mazda O, Teshigawara K, Fujimoto S, Hatlori N, Dou YM, Katsura Y: A Reporter System Using a Flow Cylometer to Detect Promoter/Enhancec Activity in Lymphoid Cell Lines. J Immunol Methods 1994, 169:53-61. Mcintyre CA, Home CJ, Lawry J, Rees RC: The Detection of Intracytoplasmic Interleukin-2 in Jurkat E6.1 and Human Peripheral Blood Moonm~lear Cells Using Direct Conjugate, Two-Colour, Immunofluorescent Flow Cytometry. J Immunol Methods 1994, 169:213-220. Nag B, Mukku PV, Arimilli S, Kendrick T, Deshpande SV, 5harma SD: Separation of Complexes OF Major Histocompatibility Class II Molecules and Known Antigenic Peptide by Metal Chelate Affinity Chromatography. J Immunol Methods 1994, 169:273-285. Ohlin M, Borrebaeck CAK: Flow Cytometric Analysis of the Stability of Antibody Production by Human X Human X Mouse Heterohybridoma Subclones. J Immunol Methods 1994, 170:75-82. Perticarari S, Presani G, Banff E: A New Flow Cytometric Assay for the Evaluation of Phagocytasis and Ihe Oxidative Burst in Whole Blood. J Immunol Methods ]994, 170:117-124. Quinlan N, Okennedy R: Enhanced Cloning Efficiencies of Murine Hyb¢idomas Using Hmnall Plasma Supplemented Medium. J Immunol Methods 1994, 171:253-258. Rabbany SY, Kusterbeck AW, Bredehorst R, Ligler FS: Effect of Antibody Density on the Displacement Kinetics OFa Flow Immunoassay. J Immunol Methods 1994, 168:227-234. Schmid I, Uittenbogaar! CH, Keld B, Giorgi Jr: A Rapid Method for Measuring Apoptnsis and DnaI-Color Immunofluorescenee by Single Laser Flow Cytometry. J Immunol Methods 1994, 170:145-157. Szucs S, Varga C, Ember I, Kertai P: The Separation OFthe Granulocytes from Different Ral Strains a Comparative Study. J Immunol Methods 1994, 167:245-251. Telford WG, King LF, Fraker PJ: Rapid Quanlilafion OFApoptnsis in Pure and Heterogeneous Cell Populations Using Flow Cytometry. J Immunol Methods 1994, 172:1-16. Vikingsson A, Pederson K, Muller D: Enumeration of IFNgamma Producing Lymphocytes by Flow Cytometry and Correlation with Quantitative Measurement of IFN-gamma. J Immunol Methods 1994, 173:219-228. Viksman MY, Liu MC, Schleimer RP, Bochner BS: Application of a Flow Cytomelric Method Using Aulofluorescence and a Tandem Fluorescent Dye to Analyze Human Alveolar Macrophage Surface Markers. J Immunol Methods 1994, 172:17-24. Wan CP, Sigh RV, Lau BHS: A Simple Fluoromelric Assay for the Determination of Cell Numbers. J Immunol Methods 1994, 173:265-272. Warrens AN, Heaton T, Sidhu S, Lornbardi G, Lechler RI: Transfected Murine Cells Expressiog HtA Class II can be used to Generate Alloreactive Human T Cell Clones. J Immunol Methods 1994, 169:25-33. Wunder E, Editor: Hemalopoietic Stem Cells, the • • Mulhouse Manual. Dayton; AlphaMod Press 1994, :. [14]. Zhang MZ, Brunnee T, Kleinetebbe I, Graf K, Oconnor A, Kunkel G: Measurement of Membrane Bound IgD and IgM on B Lymphocytes. l Immunol Methods 1993, 166:215-221.

PCR techniques Related review: Recent advances in differential display (pp 274-280) Babu JS, Kanangat S, Rouse BT: Limitations and Modifications of Quantitative Polymerase Chain Reaction - Application to Measurement of Multiple Messenger RNAs Present in Small Amounts of Sample RNA. J Immunol Methods 1993, 165:207-216. Birk PE, Grimm PC: Rapid Nonradioactive in Situ Hybridization for Interleukin-2 Mrna with Rihoprohes Generated Using the Polymerase chain Reaction. J Immunol Methods 1994, 167:83-89. Callard D, Lescure B, Mazzolini L: A Method for the • Elimination of False Positives Generated by the MRNA Differential Display Technique. BioTechniques 1994, 16:1096-1103. [34]. Cottrez F, Auriauh C, Capron A, Croux H: Analysis of the V Beta Specificity of Superantigon Activation with a Rapid and Sensitive Method Using RT PCR and an Automatic DNA Analyser. J Immunol Methods 1994, 172:85-94. Donohue PJ, Alberts GF, Hampton BS, winkles JA: • A Delayed-early Gone Activated by Fibroblast Growth Factor-1 Encodes a Protein Related Io Aldose Reducta~. J Biol Chem 1994, 269: 8606-8609. [16]. Hsu DKW, Donohue PJ, Alberts GF, Winkles JA: Fibroblast • Growth Factor-1 Induces Phosphofructokinase, Fatty Acid Synthase and Ca 2+ -ATPase MRNA Expression in NIH 3T3 Cells. Biochem Biophy Res Cumin 1993, 1 9 7 : 1 4 8 3 - 1 4 9 1 . [15]. Ito T, Kito K, Adati N, Mitusui Y, Hagiwara H, Sakai Y: • Fluorescent Differential Display: Arbitrarily Primed RT-PCR Fingerprinting on an Automated DNA Sequencer. FEBSLet] 1994, 351:321-236. [33]. Liang P, Averboukh L, Pardee AB: Method of Differential • Display. Methods in Mol Genetics 1994, 5:3-16. [28]. Liang P, Averboukh L, Zhu W, Pardee AB: Ras Activation • of Genes: Mob-1 as a Model. Proc Natl Acad Sci U S A 1994, 91:12515-12519. I8]. Liang P, Bauer D, Averboukh L, War]hoe P, Rohrwild M, • • Muller H, Strauss M, Pardee, AB: Analysis Altered Gone Expression by Differential Display. Methods Enzymol. 1995, in Press:. ]18]. Liang P, Zhu W, Zhang X, Guo Z, O'Connell RP, • • Avetboukh L, Wang E, Pardee AB: Differential Display Using One-base Anchored Oligo-dT Primers. Nucleic Acids Res 1995, in Pre~:. [25]. McClelland M , Walsh J: RNA Fingerprinting by Arbitrarily • • Primed PCR. PCR Methods Applic 1994, :$66- $81. [19]. Moonka D, Loh EY: A Comerm~ Primer to Amplify Both Alpha and Beta Chains of the Human T Cell Receptor. J Immunol Methods 1994, 169:41-51. MOU L, Miller H, Li J, Wang E, Chalifour L: Improvements • e to the Differential Display Method for Gene Analysis. Biochem Biophy Res Cumin 1994, 199:564-569. [36]. Nichols D, Andrews PC, Zhang P, Bergs]rum DE: A • UniversalNucleoside for use at Ambiguous Sites in DNA Primers. Nature 1994, 369:492-493. [29]. Pena SDJ, Barreto G, Vago AR, Demarco L, Reinach FC, Nero ED, Simpson AJG: Sequence-Sp~ific Gene SignatuRs can he Obtained by PCR with Single Specif'K: Primers at Low Slringency. Proc Natl Acad Sci U S A ]994, 91:1946-1949. Reiner SL, Zheng SC, Curry DB, Locksley RM: Constructing Polycompetitor CDNAs for Quantitative PCR. J Immunol Methods 1993, 165;:37-46. Reiner SL, Zheng SC, Curry DB, Locksley RM: Constructing Polycompetitor CDNAs for Quantitative PCR (vol 165, Pg 37, 19931. J Irnmunol Methods 1994, 173:133. Ruberti F, Catlaneo A, Bradbury A: The use of the RACE Method to Clone Hybridoma CDNA when V Region Primers Fail. J Immunol Methods 1994,173:33-39. Russell M, Utans U, Wallace AF, Liang P, Arceci MJ, • Kamovsky MJ, Wyner LR, Yamashita Y, Tam C: Identification and Upreguiation of Galactnse/NAcetylgalactosamine Macrophage Lectin in Rat Cardiac Allngrafts with Arleriosclerods. J Clio Invest 1994, 94:722-730. [10]. Schreiner T, Wiesneth M, Maccari 8, Sawodny B, Prochnowcalzia H, Kubanek B: A Simplified Rapid Method for Restriction Fragment Length Polymorphism Analysis after Bone Marrow Transplantation. J Immunol Methods 1994, 168:183-185.

Sokolov BP, Prockop DI: A Rapid and Simple PCR• basedMethod for Isolation of CDNAs from Differentially Expressed Genes. Nucleic Acids Res 1994, 22:4009-401S. [27]. Taniguchi A, Kohsaka H, Carson DA: Competitive RT-PCR ELISA - a Rapid, Sensitive and Nmi-Radioactive Method to Quantitate Cytokine Mrna. J Immunol Methods 1994, 169:101-109.

Utans U, Liang P, Wyner LR, Karnovsky MI, Russell • M: Chronic Cardiac Rejection: Identification of Five Uprngolated Genes in Transplanted Hearts by Differential MRNA Display. Proc Natl Acad Sci U S A 1994, 91:6463Jo467. [9].

Antibody-related techniques Related review: Analysis of immunoglobulin gene rearrangements in single B cells (pp 281-284) Akhoundi C, Rocbet N, Ferrua B, Rossi B: Production and Characterization of Monoclonal and Polyclonal Antibodies to Human Alpha 2-HS: Development of a Two-site ELISATest. J Immunol Methods 1994, 172:189-196. Alon R, Bayer EA, Wilcbek M: Affinity Cleavage of Cell Surface Antibodies Using the Avidin-Biofin System. J Immunol Methods 1993, 165:127-134. Asseman C, Pancre V, Delanoye A, Capron A, Auriauh C: A Radiolmmunoamay for the Quantification of Human Ubiquifin in Biological Fluids: Application to Parasitic and Allergic Diseases. J Immunol Methods 1994, 173:93-101. Auriol ], Guesclon JL, Mazie JC, Nato F: Development of a Bispecific Moonclonal Antibody for use in Molecular Hybrldisation. J Immunol Methods 1994, 169:123-133. Baker MD, Kam HA, Read LR: Restoration ofa Nomtal Level of Immunoglobulin Production in a HybHdoma Cell Line Following Modif'~ation of the Chromosomal I ~ o g l o b u l i n Mo Gene by Gene Replacement. J Immunol Methods 1994, 168:25-32. Bakkali L, Guillou R, Gonzague M, Cruciere C: A Rapid and Sensitive Chemiluminescence DotImnmnobinding Assay for Screening HybHdoma Supernatants. J Immunol Methods 1994, 170:177-184.

Ball JM, Henry NL, Monlelaro RC, Newman MJ: A Versatile Synthetic Peptide-based ELISAFO¢ Identifying Antibody Epitopes. J Immunol Methods 1994, 171:37-44. Balogh P, Szeketes G, Nemeth P: Hapten-Mndiated Idantif'w.ation of Cell Membrane Antigens U~ng an Anti-FITC Monodonal Antibody. J Immunol Methods 1994, 169:35-40. Baslund B, Wieslander J: A Rapid and Semi-Quantitative Method for the Detection of Autoantibodles by Multiple Spot Immonoassay. J Immunol Methods 1994, 169:183-193. Beavis AJ, Pennline KJ: Tracking of Murine Spleen Cells in Vivo - Detection of Pkh26-Laheled Cells in the Pancreas of Non-Obnse Diabetic (Nod) Mice. J Immunol Methods 1994, 170:57-65. Beavis AJ, Pennline KJ: Tracking of Murine Spleen Cells in Vivo - Detection of Pkh26-Laheled Cells in the Pancreas of Non-Ohese Diabetic (Nod) Mice. J Immunol Methods 1994, 170:57~5. Beer DJ, Yates AM, Jack GW: Development of Enzymelinked Immunnsorhent Assays (ELISAs) for the Detection of Monoclonal Antibody Leakage from Immonoaffinity Chromatography Malrlces. J Immunol Methods 1994, 173:103-109. Berghman LR, Grauwels L, Vanhamme L, Vandesande F: A Simple Method for the Immuno¢ytuchemical Proce~ing of Large Numbers of Floating Sections and Its Application in Screening for Monuclonal Antibodies. J Immunol Methods 1994, 168:197-202. Beny MJ, Wattam TAK, Willels J, Lindner N, Degraaf T, Hunt 1-, Gani M, Davis PJ, Porter P: Assay and Purification of Fv Fragments in Fermenter Cultures - Design and Evaluation of Generic Binding Reagents. J Immunol Methods 1994, 167:173-182. Boe A, Canosi U, Donini S, Mastrangeli R, Ythier A, Crescenzi US: Determination of Haptogfobin Expression in IL-6 Treated HepG2 Cells by ELISA and by RNA Hybridization -Evaluation of a Quantitative Method to Measure IL-6. J Immunol Methods ]994, 171:157-167. Brisco MJ, Condon J, Hughes E, Neoh SH, Sykes PJ, • SeshardiR, Toogood I, Waters K, Taoro G, Ekert H, Morley AA: Outcome Prediction in Childhood Acute Lymphoblastic Leukaemia by Molecular Quantification of Residual Disease at the End of Induction. Lancet 1994, 343:196-200. [17]. Callan MFC, Reyburn HT, Bowness P, Ottenhoff TH, Engel I, Klausner RD, Bell JI, Mcmichael A: A Method for Producing Mormclonal Antibodies to Human T-Cell-Receptor Beta-Chain Variable Regions. Proc Natl Acad Sci U S A 1993, 90:10454-1045B. Carayannopoulos L, Max EE, Capra JD: Recombinant Human IgA Expressed in Insect Cells. Pro( Natl Acad Sci U S A 1994, 91:8348-8352.

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