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Immunophenotypic Characterisation of Peripheral Blood Mononuclear Cells in Patients With MDR Pulmonary TB
Chest Infections SESSION TITLE: Tuberculosis SESSION TYPE: Original Investigation Poster PRESENTED ON: Wednesday, October 26, 2016 at 01:30 PM - 02:30 PM
Immunophenotypic Characterisation of Peripheral Blood Mononuclear Cells in Patients With MDR Pulmonary TB Hamdy Mohammadien MD* Abeer Mohamad MD; and Ahmad Abdel-Aziz MD Sohag Faculty of Medicine, Sohag University, Sohag, Egypt PURPOSE: To determine changes that take place in the sub populations of peripheral blood mononuclear cells of patients with MDR- tuberculosis and to compare their immune profile to responders to anti- tuberculosis drugs and healthy controls. METHODS: Flow cytometry was used to determine the absolute numbers and percentages of T CD3, T CD4, T CD8, T CD19, T CD14, T CD25 and T CD56 cells in 25 patients with active pulmonary TB responding to treatment,15 MDR TB and in 20 healthy volunteers.
CHEST INFECTIONS
RESULTS: There were significant differences in the values of CD3, CD4, CD19, and CD56 T cells among the groups. CD4 was significantly higher in controls than Responders (39.5 5.6 vs 33.5 6, P ¼ 0.002), and lower in Responders than MDR but not significant (33.5 6 vs 38.1 4.1, P ¼ 0.06). CD3 was significantly lower in MDR than Responders and controls (45.12 6.6 vs 52. 11.4& 66.2 5.7, P ¼ 0.0001). CD19 was significantly higher in Responders than MDR and Controls (P ¼ 0.04 & 0.03), lower in MDR than Controls but not significant (5.72 1.7 vs 6.61 4.2, p¼0.8). CD56 was significantly higher in MDR and Responders than Controls (P ¼ 0.007 & 0.02), also higher in MDR than Responders but not significant (14.78.1 vs 9.60 6.2, p¼0.07). There were no significant differences in the values of CD4 CD8, CD4 CD25, CD8, CD8 HLA and CD14 between all groups, but CD8 was higher in Responders than MDR & Controls without significant difference (26.9 11.6 vs 19.7 2.3 & 25 3.9, p¼0.5), also CD8 HLA was higher in MDR than Responders & Controls but not significant (29.4 9.2 vs 24.8 13.4,& 23 11.2, p¼0.2) CONCLUSIONS: There are significant changes in the cellular immune response particularly affecting the CD3, CD4, CD19 and CD56 T cells in MDR pulmonary TB. Therefore, further studies of these changes may have important implications on the development of diagnostic tools and treatment modalities. CLINICAL IMPLICATIONS: In addition, there is a need to further evaluate the mechanisms leading to these changes so as to understand the pathogenesis and prognostic markers of the disease and to develop immunomodulatory modalities of therapy. DISCLOSURE: The following authors have nothing to disclose: Hamdy Mohammadien, Abeer Mohamad, Ahmad Abdel-Aziz No Product/Research Disclosure Information DOI:
http://dx.doi.org/10.1016/j.chest.2016.08.221
Copyright ª 2016 American College of Chest Physicians. Published by Elsevier Inc. All rights reserved.
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150#4S CHEST OCTOBER 2016
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Immunophenotypic Characterisation of Peripheral Blood Mononuclear Cells in Patients With MDR Pulmonary TB Hamdy Mohammadien MD* Abeer Mohamad MD; and Ahmad Abdel-Aziz MD Sohag Faculty of Medicine, Sohag University, Sohag, Egypt PURPOSE: To determine changes that take place in the sub populations of peripheral blood mononuclear cells of patients with MDR- tuberculosis and to compare their immune profile to responders to anti- tuberculosis drugs and healthy controls. METHODS: Flow cytometry was used to determine the absolute numbers and percentages of T CD3, T CD4, T CD8, T CD19, T CD14, T CD25 and T CD56 cells in 25 patients with active pulmonary TB responding to treatment,15 MDR TB and in 20 healthy volunteers.
CHEST INFECTIONS
RESULTS: There were significant differences in the values of CD3, CD4, CD19, and CD56 T cells among the groups. CD4 was significantly higher in controls than Responders (39.5 5.6 vs 33.5 6, P ¼ 0.002), and lower in Responders than MDR but not significant (33.5 6 vs 38.1 4.1, P ¼ 0.06). CD3 was significantly lower in MDR than Responders and controls (45.12 6.6 vs 52. 11.4& 66.2 5.7, P ¼ 0.0001). CD19 was significantly higher in Responders than MDR and Controls (P ¼ 0.04 & 0.03), lower in MDR than Controls but not significant (5.72 1.7 vs 6.61 4.2, p¼0.8). CD56 was significantly higher in MDR and Responders than Controls (P ¼ 0.007 & 0.02), also higher in MDR than Responders but not significant (14.78.1 vs 9.60 6.2, p¼0.07). There were no significant differences in the values of CD4 CD8, CD4 CD25, CD8, CD8 HLA and CD14 between all groups, but CD8 was higher in Responders than MDR & Controls without significant difference (26.9 11.6 vs 19.7 2.3 & 25 3.9, p¼0.5), also CD8 HLA was higher in MDR than Responders & Controls but not significant (29.4 9.2 vs 24.8 13.4,& 23 11.2, p¼0.2) CONCLUSIONS: There are significant changes in the cellular immune response particularly affecting the CD3, CD4, CD19 and CD56 T cells in MDR pulmonary TB. Therefore, further studies of these changes may have important implications on the development of diagnostic tools and treatment modalities. CLINICAL IMPLICATIONS: In addition, there is a need to further evaluate the mechanisms leading to these changes so as to understand the pathogenesis and prognostic markers of the disease and to develop immunomodulatory modalities of therapy. DISCLOSURE: The following authors have nothing to disclose: Hamdy Mohammadien, Abeer Mohamad, Ahmad Abdel-Aziz No Product/Research Disclosure Information DOI:
http://dx.doi.org/10.1016/j.chest.2016.08.221
Copyright ª 2016 American College of Chest Physicians. Published by Elsevier Inc. All rights reserved.
212A
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150#4S CHEST OCTOBER 2016
]