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immunogenetic correlations
Abstracts / Journal of the Neurological Sciences 333 (2013) e358–e421
Patients and methods: Thirty-four Japanese patients with CIS were consecutively enrolled in this prospective study conducted from Feb 2006 to July 2012 with a mean follow up period of 38 months. Antiaquaporin (AQP) 4 antibodies were examined using a cell-based assay. Results: Five patients developed MS (14.7%); of these, 2 revealed relapses of the different lesions and 3 revealed other lesions on brain MRI. Moreover 21 were classified as still having CIS (61.8%). The remaining 8 patients had a neuromyelitis optica-related disorder (NMOrd); 3 patients demonstrated anti-AQP4 antibodies, and 5 revealed a centrally located long spinal cord lesion on MRI (1 patient demonstrated both lesions). OCBs were observed in CSF at the first visit in 2 of 5 (40%), 3 of 21 (14.3%), and 3 of 8 (37.5%) patients who revealed conversion to MS, nonconversion, and NMOrd, respectively. Conclusion: Two of 5 patients with CIS and OCBs developed MS for only for 38 months; moreover, NMOrd was observed in 8 of 34 with CIS (23.5%). This study suggests that CSF-OCBs could be helpful in predicting MS development in Japanese patients with a low incidence of OCB (47.6% of different our series) and that Japanese patients with CIS have a low risk of MS development. doi:10.1016/j.jns.2013.07.1429
Abstract — WCN 2013 No: 1845 Topic: 6 — MS & Demyelinating Diseases Impact of HLA DRB1*1501 gene and oligoclonal bands in multiple sclerosis. Clinical radiological/immunogenetic correlations S. Daoudia, M. Lounisa, M. Ait-Kaci-Ahmedb. aDepartment of Neurology, Nédir Mohamed Hospital, Tizi-Ouzou, Algeria; bDepartment of Neurology, Ait Idir Hospital, Algiers, Algeria Introduction: The aim of this study was to determine the value of immunogenetic risk factors and estimate their relationship with clinical and radiological characteristics and disability status of patients with multiple sclerosis in a population in northern Algeria. Material and methods: This is a prospective study concerning 60 patients with MS. We noted the age, sex, and clinical manifestations since the beginning of the disease. Paraclinical analyzes systematically included in our patients, immunology of CSF, brain and spine MRI. We evaluated the frequency of HLA DRB1*1501. Results: The mean age was 34.4 years with a sex ratio F/M = 2. The HLA DRB1*1501 was positive in 30% of cases, with an odds ratio of 2.3. In the HLA DRB1*1501 positive group, 38.9% were male and 26.2% were female. We observed the presence of oligoclonal bands in 85% of patients, 25% of the HLA DRB1*1501 positive group. No significant association between clinical and genetics has been found. The brain stem lesion on MRI was associated with the presence of HLA DRB1*1501 (P b 0.01). The relapsing multiple sclerosis represented 83.3% of the HLA DRB1*1501 positive group. The mean EDSS score was 1.2 in the HLA DRB1*1501 positive group and 0.91 in the negative group (P = 0.41). Conclusion: The presence of oligoclonal bands in the CSF was associated with many exacerbations of the disease. The brain stem-lesion on MRI was significantly associated with the presence of HLA DRB1*1501.
doi:10.1016/j.jns.2013.07.1430
Abstract — WCN 2013 No: 1715 Topic: 6 — MS & Demyelinating Diseases Uric acid serum levels change in the earliest phase of demyelization S. Ljubisavljevica, I. Stojanovica, S. Vojinovica, M. Milojkovica, O. Dunjica, V. Djuricb, A. Prazicb, D. Stojanova, D. Pavlovica. aUniversity of Nis,
e395
Faculty of Medicine, Nis, Serbia; bClinical Centre of Nis, Clinic for Neurology, Nis, Serbia Objectives and methods: In order to examine the endogenous antioxidant values in the earliest phase of demyelization, we have determined uric acid (UA) serum values in the patients with clinically isolated syndrome (CIS) and relapsing remitting multiple sclerosis (RRMS), regarding their clinical disability, measured by Extended Disability Status Scale (EDSS), Magnetic Resonance Imaging (MRI), disease duration, gender and other parameters. Results: The UA levels were lower in CIS and RRMS patients than in control group, whether male or female (p b 0.05). The UA levels were decreased in RRMS compared to CIS patients (p b 0.05). Regarding EDSS, MRI and disease duration, obtained values of UA were higher in both study groups in patients with lower EDSS, lower MRI lesion number and shorter disease duration (p b 0.05). The greatest significance in decreased UA levels was observed in female compared to male patients, in both study groups (p b 0.05). The results suggest negative linear correlation between UA levels and disease duration, EDSS and MRI in CIS (p b 0.01), with the same correlation between UA levels and disease duration in RRMS patients (p b 0.01). There was also significant correlation between UA levels and EDSS in RRMS patients (p b 0.01). Conclusions: The obtained results point to the importance of endogenous antioxidants, UA, in the outbreak and course of neuroinflammation. This could be favorable for the new pathogenetically conditioned neuroinflammatory therapy concepts which do not initially rely only on immunomodulatory, but also on the antioxidative effects. doi:10.1016/j.jns.2013.07.1431
Abstract — WCN 2013 No: 1519 Topic: 6 — MS & Demyelinating Diseases Cerebrospinal fluid transferrin levels are reduced in patients with early multiple sclerosis M. Khalila, B. Riedlbauera, C. Langkammera, C. Enzingera, S. Ropelea, T. Stojakovica, H. Scharnagla, V. Culeaa, A. Petzoldb, J.J. Archelosa, S. Fuchsa, F. Fazekasa. aMedical University of Graz, Graz, Austria; b Free University Medical Center, Amsterdam, The Netherlands Background: Abnormally high cerebral iron deposition has been described in multiple sclerosis (MS), which is mainly evidenced by applying advanced MRI techniques. Up to now it is not completely clear whether alterations of iron metabolism markers in body fluids are present in MS and if these markers are related to clinical and imaging parameters. Objective: We aimed to investigate if iron metabolism markers in cerebrospinal fluid (CSF) and serum of patients with clinically isolated syndromes (CIS) and MS differ from those of control patients with other neurologic diseases of non-inflammatory aetiology (NC). Methods: We included non-anaemic patients with CIS/MS (n = 77, CIS 62; MS 15) and 69 NC. None of the patients received any immunomodulatory treatment at time of lumbar puncture. We nephelometrically assessed serum levels of ferritin, transferrin and soluble transferrin receptor and CSF levels of ferritin and transferrin. The serum transferrin saturation was calculated from serum ferritin and transferrin levels. Results: CSF transferrin levels were significantly reduced in CIS/MS compared to NC (p b 0.001). Higher CSF transferrin levels correlated with lower physical disability (r = −0.3, p b 0.01). There were no significant differences comparing CIS/MS and NC regarding CSF ferritin and serum ferritin, transferrin, soluble transferrin receptor and the transferrin saturation. Conclusion: Our results indicate that CSF transferrin levels are altered in early phases of MS with some relation to physical disability. Further longitudinal studies are currently undertaken to investigate if CSF
Patients and methods: Thirty-four Japanese patients with CIS were consecutively enrolled in this prospective study conducted from Feb 2006 to July 2012 with a mean follow up period of 38 months. Antiaquaporin (AQP) 4 antibodies were examined using a cell-based assay. Results: Five patients developed MS (14.7%); of these, 2 revealed relapses of the different lesions and 3 revealed other lesions on brain MRI. Moreover 21 were classified as still having CIS (61.8%). The remaining 8 patients had a neuromyelitis optica-related disorder (NMOrd); 3 patients demonstrated anti-AQP4 antibodies, and 5 revealed a centrally located long spinal cord lesion on MRI (1 patient demonstrated both lesions). OCBs were observed in CSF at the first visit in 2 of 5 (40%), 3 of 21 (14.3%), and 3 of 8 (37.5%) patients who revealed conversion to MS, nonconversion, and NMOrd, respectively. Conclusion: Two of 5 patients with CIS and OCBs developed MS for only for 38 months; moreover, NMOrd was observed in 8 of 34 with CIS (23.5%). This study suggests that CSF-OCBs could be helpful in predicting MS development in Japanese patients with a low incidence of OCB (47.6% of different our series) and that Japanese patients with CIS have a low risk of MS development. doi:10.1016/j.jns.2013.07.1429
Abstract — WCN 2013 No: 1845 Topic: 6 — MS & Demyelinating Diseases Impact of HLA DRB1*1501 gene and oligoclonal bands in multiple sclerosis. Clinical radiological/immunogenetic correlations S. Daoudia, M. Lounisa, M. Ait-Kaci-Ahmedb. aDepartment of Neurology, Nédir Mohamed Hospital, Tizi-Ouzou, Algeria; bDepartment of Neurology, Ait Idir Hospital, Algiers, Algeria Introduction: The aim of this study was to determine the value of immunogenetic risk factors and estimate their relationship with clinical and radiological characteristics and disability status of patients with multiple sclerosis in a population in northern Algeria. Material and methods: This is a prospective study concerning 60 patients with MS. We noted the age, sex, and clinical manifestations since the beginning of the disease. Paraclinical analyzes systematically included in our patients, immunology of CSF, brain and spine MRI. We evaluated the frequency of HLA DRB1*1501. Results: The mean age was 34.4 years with a sex ratio F/M = 2. The HLA DRB1*1501 was positive in 30% of cases, with an odds ratio of 2.3. In the HLA DRB1*1501 positive group, 38.9% were male and 26.2% were female. We observed the presence of oligoclonal bands in 85% of patients, 25% of the HLA DRB1*1501 positive group. No significant association between clinical and genetics has been found. The brain stem lesion on MRI was associated with the presence of HLA DRB1*1501 (P b 0.01). The relapsing multiple sclerosis represented 83.3% of the HLA DRB1*1501 positive group. The mean EDSS score was 1.2 in the HLA DRB1*1501 positive group and 0.91 in the negative group (P = 0.41). Conclusion: The presence of oligoclonal bands in the CSF was associated with many exacerbations of the disease. The brain stem-lesion on MRI was significantly associated with the presence of HLA DRB1*1501.
doi:10.1016/j.jns.2013.07.1430
Abstract — WCN 2013 No: 1715 Topic: 6 — MS & Demyelinating Diseases Uric acid serum levels change in the earliest phase of demyelization S. Ljubisavljevica, I. Stojanovica, S. Vojinovica, M. Milojkovica, O. Dunjica, V. Djuricb, A. Prazicb, D. Stojanova, D. Pavlovica. aUniversity of Nis,
e395
Faculty of Medicine, Nis, Serbia; bClinical Centre of Nis, Clinic for Neurology, Nis, Serbia Objectives and methods: In order to examine the endogenous antioxidant values in the earliest phase of demyelization, we have determined uric acid (UA) serum values in the patients with clinically isolated syndrome (CIS) and relapsing remitting multiple sclerosis (RRMS), regarding their clinical disability, measured by Extended Disability Status Scale (EDSS), Magnetic Resonance Imaging (MRI), disease duration, gender and other parameters. Results: The UA levels were lower in CIS and RRMS patients than in control group, whether male or female (p b 0.05). The UA levels were decreased in RRMS compared to CIS patients (p b 0.05). Regarding EDSS, MRI and disease duration, obtained values of UA were higher in both study groups in patients with lower EDSS, lower MRI lesion number and shorter disease duration (p b 0.05). The greatest significance in decreased UA levels was observed in female compared to male patients, in both study groups (p b 0.05). The results suggest negative linear correlation between UA levels and disease duration, EDSS and MRI in CIS (p b 0.01), with the same correlation between UA levels and disease duration in RRMS patients (p b 0.01). There was also significant correlation between UA levels and EDSS in RRMS patients (p b 0.01). Conclusions: The obtained results point to the importance of endogenous antioxidants, UA, in the outbreak and course of neuroinflammation. This could be favorable for the new pathogenetically conditioned neuroinflammatory therapy concepts which do not initially rely only on immunomodulatory, but also on the antioxidative effects. doi:10.1016/j.jns.2013.07.1431
Abstract — WCN 2013 No: 1519 Topic: 6 — MS & Demyelinating Diseases Cerebrospinal fluid transferrin levels are reduced in patients with early multiple sclerosis M. Khalila, B. Riedlbauera, C. Langkammera, C. Enzingera, S. Ropelea, T. Stojakovica, H. Scharnagla, V. Culeaa, A. Petzoldb, J.J. Archelosa, S. Fuchsa, F. Fazekasa. aMedical University of Graz, Graz, Austria; b Free University Medical Center, Amsterdam, The Netherlands Background: Abnormally high cerebral iron deposition has been described in multiple sclerosis (MS), which is mainly evidenced by applying advanced MRI techniques. Up to now it is not completely clear whether alterations of iron metabolism markers in body fluids are present in MS and if these markers are related to clinical and imaging parameters. Objective: We aimed to investigate if iron metabolism markers in cerebrospinal fluid (CSF) and serum of patients with clinically isolated syndromes (CIS) and MS differ from those of control patients with other neurologic diseases of non-inflammatory aetiology (NC). Methods: We included non-anaemic patients with CIS/MS (n = 77, CIS 62; MS 15) and 69 NC. None of the patients received any immunomodulatory treatment at time of lumbar puncture. We nephelometrically assessed serum levels of ferritin, transferrin and soluble transferrin receptor and CSF levels of ferritin and transferrin. The serum transferrin saturation was calculated from serum ferritin and transferrin levels. Results: CSF transferrin levels were significantly reduced in CIS/MS compared to NC (p b 0.001). Higher CSF transferrin levels correlated with lower physical disability (r = −0.3, p b 0.01). There were no significant differences comparing CIS/MS and NC regarding CSF ferritin and serum ferritin, transferrin, soluble transferrin receptor and the transferrin saturation. Conclusion: Our results indicate that CSF transferrin levels are altered in early phases of MS with some relation to physical disability. Further longitudinal studies are currently undertaken to investigate if CSF