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IMPACT OF LOW-LEVEL LASER THERAPY ON NITRIC OXIDE PRODUCTION AND CELL VIABILITY
OOOO Volume 124, Number 2
ABSTRACTS Abstracts e141
Conclusions: Surgical decompression was useful in reducing the volume of the inflammatory periapical cysts before surgical enucleation. The immunohistochemical analysis of the specimens obtained previously to and after surgical decompression did not show a standard pattern of decreasing of pro-inflammatory markers or increasing of tissue repair markers on the periapical cysts included in the studied sample.
Results: The WST based program enabled the recovery of all clinical data of approximately 200 patients registered in EW in only 2 minutes. Conclusion: The use of this WST based program facilitates the evaluation, interpretation and research of clinical data by EW users. Besides that, the program might be useful for future Brazilian multicenter studies in oral medicine.
RP83 - IMPACT OF LOW-LEVEL LASER THERAPY ON NITRIC OXIDE PRODUCTION AND CELL VIABILITY. JAIR CARNEIRO LEAO, SAMANTHA CARDOSO DE ANDRADE, IGOR HENRIQUE MORAIS SILVA, ALESSANDRA DE ALBUQUERQUE TAVARES CARVALHO, LUIZ ALCINO MONTEIRO GUEIROS, WYLLA TATIANA FERREIRA E SILVA, RAUL MANHÃES DE CASTRO.
RP85 - SUPPRESSION OF MAGE-A10 REDUCES METASTATIC PHENOTYPE OF TONGUE SQUAMOUS CELL CARCINOMA CELLS. BRUNA DOS SANTOS MENDONÇA, MICHELLE AGOSTINI, IARA GONÇALVES DE AQUINO, DÉBORA CAMPANELLA BASTOS, FRANKLIN DAVID RUMJANEK.
The aim of this study was to evaluate the effect of LLLT in the synthesis of NO and cellular viability of cultured macrophages. Study Design: RAW 264.7 cell line were cultivated into 96-well plates that were irradiated using an a As-Ga-Al laser at a wavelength of 660 nm, power of 30, 50 and 100 mW, with duration of 10, 30 and 60 seconds. Basal NO production was measured as was its production in cells stimulated by lipopolysaccharide (LPS) with or without laser irradiation. NO levels were determined by assaying nitrite levels. RAW 264.7 cell viability was quantified by MTT. Results: LLLT significantly increased (P<0,001) basal NO production by both LPS-stimulated and non-stimulated RAW 264.7 cells when compared with non-laser stimulated cells. In addition, NO production was increased to the greatest extent upon application of an energy density of 32 J/cm2 (P<.011). Also, analysis of irradiation duration revealed no significant (P>.05) difference in power usage. Concerning cell viability, it was not observed any change in cell culture. Conclusions: These data indicate that irradiation at 660 nm increases NO production by macrophages in culture when compared with non-stimulated cells, with no effect on the viability of the irradiated cells.
RP84 - WEB SCRAPING COMPUTER PROGRAM FOR THE ESTOMATO WEB SOFTWARE: A POTENTIAL TOOL FOR ORAL MEDICINE PRACTICE AND RESEARCH. CAROLINE FRANCO ZANON, MARINA BERNARDES GRILLO DE BRITO, MARIANA GONSALES LUVIZUTTO, ELLEN BRILHANTE A. CORTEZZI, BRUNO AUGUSTO BENEVENUTO DE ANDRADE, MARIO JOSÉ ROMAÑACH, MICHELLE AGOSTINI. The Estomato Web (EW) software is provided and supported by the Brazilian Society of Stomatology and Oral Pathology (SOBEP). EW is currently used in 17 Brazilian Oral Medicine services, allowing to record digital data of patients and quick searches of a limited number of clinical parameters. However, there is no available tool for extracting and exporting a large number of digital data at once. The aim of this study was to create a computer program to automate the reading of all clinical data registered on EW and to export them for files in Excel spreadsheets. Study design. The creation and use of the computer program was allowed by SOBEP; it was developed using the Web Scraping Technique (WST), which consists on automated navigation of pages from a website, with the possibility to collect the content displayed on each page.
Melanoma antigen family A10 (MAGE-A10) is a cancertestis antigen down-regulated in somatic adult tissues, but aberrantly expressed in various malignancies. Proteomic studies of our group showed higher levels of MAGE-A10 in the metastatic cell line SCC-9 LN-2 compared with its parental primary human tongue squamous cell carcinoma cell line SCC-9. The aim of this study was to evaluate the role of MAGE-A10 on the metastatic phenotype of SCC-9 LN-2 cells. Study Design: MAGE-A10 was suppressed by specific shRNAs and its effects on the expression of known epithelialmesenchymal transition (EMT) markers, migration, cell motility, invasion and adhesion were analyzed. Results: MAGE-A10 suppression induced morphological changes in SCC-9 LN-2 cells and was able to modulate the expression of the EMT markers, causing reduction of mRNA and protein levels of N-caderin and vimentin and increase of E-caderin levels. In vitro migration, cell motility and invasion were also reduced. In contrast, cell-cell adhesion was increased. Conclusion: MAGE-A10 suppression reduces metastatic phenotype of SCC-9 LN-2 cells. Further studies are required to consider MAGE-A10 as a potential prognostic and immunotherapeutic target for tongue squamous cell carcinoma.
RP86 - ORAL LESIONS FREQUENCY IN BRAZILIAN CHILDREN. HIDECAZIO DE OLIVEIRA SOUSA, LAINE RIBEIRO ANTONELLI, GISELLA MATOS DA SILVA WHITE, ENEIDA FRANCO VÊNCIO. To investigate oral lesions frequency in children in a Brazilian region. Study Design: Clinicopathologic data were retrieved in a Brazilian population and collected information regarding gender, age, location and histopathologic diagnoses. The patients were grouped according to dentition in 0-5, 6-10 and 11-14 age groups. The lesions were categorized into: non-neoplastic lesions, benign neoplasms and malignant neoplasms. Results: From a total of 8081 lesions, 10.93% were in children. Female represented 54.13%. The age ranged from 1 month to 14 years (mean 9.94 years), with 11-14 age groups predominance (50.73%), followed by 6-10 (39.19%) and 0-5 (10.08%). Regarding location, mandible was the most affected with 30.02%, followed by maxilla (25.92%) and lips (25.82%) (P<.0001). Non-neoplastic lesions were the most common category with 80.40% (P¼.0133), followed by benign neoplasms (18.46%) and malignant neoplasms (1.14%). The most frequent lesions, in general, were mucocele, fibrous inflammatory hyperplasia and dentigerous cyst, with 24.24%, 11.10% and 10.08% respectively.
ABSTRACTS Abstracts e141
Conclusions: Surgical decompression was useful in reducing the volume of the inflammatory periapical cysts before surgical enucleation. The immunohistochemical analysis of the specimens obtained previously to and after surgical decompression did not show a standard pattern of decreasing of pro-inflammatory markers or increasing of tissue repair markers on the periapical cysts included in the studied sample.
Results: The WST based program enabled the recovery of all clinical data of approximately 200 patients registered in EW in only 2 minutes. Conclusion: The use of this WST based program facilitates the evaluation, interpretation and research of clinical data by EW users. Besides that, the program might be useful for future Brazilian multicenter studies in oral medicine.
RP83 - IMPACT OF LOW-LEVEL LASER THERAPY ON NITRIC OXIDE PRODUCTION AND CELL VIABILITY. JAIR CARNEIRO LEAO, SAMANTHA CARDOSO DE ANDRADE, IGOR HENRIQUE MORAIS SILVA, ALESSANDRA DE ALBUQUERQUE TAVARES CARVALHO, LUIZ ALCINO MONTEIRO GUEIROS, WYLLA TATIANA FERREIRA E SILVA, RAUL MANHÃES DE CASTRO.
RP85 - SUPPRESSION OF MAGE-A10 REDUCES METASTATIC PHENOTYPE OF TONGUE SQUAMOUS CELL CARCINOMA CELLS. BRUNA DOS SANTOS MENDONÇA, MICHELLE AGOSTINI, IARA GONÇALVES DE AQUINO, DÉBORA CAMPANELLA BASTOS, FRANKLIN DAVID RUMJANEK.
The aim of this study was to evaluate the effect of LLLT in the synthesis of NO and cellular viability of cultured macrophages. Study Design: RAW 264.7 cell line were cultivated into 96-well plates that were irradiated using an a As-Ga-Al laser at a wavelength of 660 nm, power of 30, 50 and 100 mW, with duration of 10, 30 and 60 seconds. Basal NO production was measured as was its production in cells stimulated by lipopolysaccharide (LPS) with or without laser irradiation. NO levels were determined by assaying nitrite levels. RAW 264.7 cell viability was quantified by MTT. Results: LLLT significantly increased (P<0,001) basal NO production by both LPS-stimulated and non-stimulated RAW 264.7 cells when compared with non-laser stimulated cells. In addition, NO production was increased to the greatest extent upon application of an energy density of 32 J/cm2 (P<.011). Also, analysis of irradiation duration revealed no significant (P>.05) difference in power usage. Concerning cell viability, it was not observed any change in cell culture. Conclusions: These data indicate that irradiation at 660 nm increases NO production by macrophages in culture when compared with non-stimulated cells, with no effect on the viability of the irradiated cells.
RP84 - WEB SCRAPING COMPUTER PROGRAM FOR THE ESTOMATO WEB SOFTWARE: A POTENTIAL TOOL FOR ORAL MEDICINE PRACTICE AND RESEARCH. CAROLINE FRANCO ZANON, MARINA BERNARDES GRILLO DE BRITO, MARIANA GONSALES LUVIZUTTO, ELLEN BRILHANTE A. CORTEZZI, BRUNO AUGUSTO BENEVENUTO DE ANDRADE, MARIO JOSÉ ROMAÑACH, MICHELLE AGOSTINI. The Estomato Web (EW) software is provided and supported by the Brazilian Society of Stomatology and Oral Pathology (SOBEP). EW is currently used in 17 Brazilian Oral Medicine services, allowing to record digital data of patients and quick searches of a limited number of clinical parameters. However, there is no available tool for extracting and exporting a large number of digital data at once. The aim of this study was to create a computer program to automate the reading of all clinical data registered on EW and to export them for files in Excel spreadsheets. Study design. The creation and use of the computer program was allowed by SOBEP; it was developed using the Web Scraping Technique (WST), which consists on automated navigation of pages from a website, with the possibility to collect the content displayed on each page.
Melanoma antigen family A10 (MAGE-A10) is a cancertestis antigen down-regulated in somatic adult tissues, but aberrantly expressed in various malignancies. Proteomic studies of our group showed higher levels of MAGE-A10 in the metastatic cell line SCC-9 LN-2 compared with its parental primary human tongue squamous cell carcinoma cell line SCC-9. The aim of this study was to evaluate the role of MAGE-A10 on the metastatic phenotype of SCC-9 LN-2 cells. Study Design: MAGE-A10 was suppressed by specific shRNAs and its effects on the expression of known epithelialmesenchymal transition (EMT) markers, migration, cell motility, invasion and adhesion were analyzed. Results: MAGE-A10 suppression induced morphological changes in SCC-9 LN-2 cells and was able to modulate the expression of the EMT markers, causing reduction of mRNA and protein levels of N-caderin and vimentin and increase of E-caderin levels. In vitro migration, cell motility and invasion were also reduced. In contrast, cell-cell adhesion was increased. Conclusion: MAGE-A10 suppression reduces metastatic phenotype of SCC-9 LN-2 cells. Further studies are required to consider MAGE-A10 as a potential prognostic and immunotherapeutic target for tongue squamous cell carcinoma.
RP86 - ORAL LESIONS FREQUENCY IN BRAZILIAN CHILDREN. HIDECAZIO DE OLIVEIRA SOUSA, LAINE RIBEIRO ANTONELLI, GISELLA MATOS DA SILVA WHITE, ENEIDA FRANCO VÊNCIO. To investigate oral lesions frequency in children in a Brazilian region. Study Design: Clinicopathologic data were retrieved in a Brazilian population and collected information regarding gender, age, location and histopathologic diagnoses. The patients were grouped according to dentition in 0-5, 6-10 and 11-14 age groups. The lesions were categorized into: non-neoplastic lesions, benign neoplasms and malignant neoplasms. Results: From a total of 8081 lesions, 10.93% were in children. Female represented 54.13%. The age ranged from 1 month to 14 years (mean 9.94 years), with 11-14 age groups predominance (50.73%), followed by 6-10 (39.19%) and 0-5 (10.08%). Regarding location, mandible was the most affected with 30.02%, followed by maxilla (25.92%) and lips (25.82%) (P<.0001). Non-neoplastic lesions were the most common category with 80.40% (P¼.0133), followed by benign neoplasms (18.46%) and malignant neoplasms (1.14%). The most frequent lesions, in general, were mucocele, fibrous inflammatory hyperplasia and dentigerous cyst, with 24.24%, 11.10% and 10.08% respectively.