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Improved Vectors for Nisin-Controlled Expression in Gram-Positive Bacteria
Plasmid 45, 61 (2001) doi:10.1006/plas.2000.1513, available online at http://www.academicpress.com on
ERRATUM Volume 44, Number 2 (2000), in the short communication “Improved Vectors for Nisin-Controlled Expression in Gram-Positive Bacteria,” by Edward M. Bryan, Taeok Bae, Michiel Kleerebezem, and Gary M. Dunny, pages 183–190 (doi: 10.1006/plas.2000.1484): On page 186, Fig. 1 contains an incorrect size and some restriction cleavage sites that do not actually exist in two of the vectors. For the reader’s convenience, the correct figure with its legend is printed here.
FIG.1. Plasmid construction. (A) Maps and construction scheme for pMSP3535. Black arrows represent antibiotic resistance markers and gram-positive bacterial replication genes. E. coli genes are shown as boxed arrows, and nisin-associated genes and promoters are shown as gray arrows. Selected restriction enzyme cleavage sites are shown. A map of pMSP3535VA is also shown, but its construction scheme is not shown (see text). (B) Selected portions of the promoter region and multicloning site are shown for pMSP3535 and pMSP3535VA, as well as for pMSP3545. The putative –10 promoter regions are TACAAT. C represents the transcriptional start site. ATG is the translational start site of nisA, Nucleotide sequence encoding the signal sequence and one-third of the mature NisA (nisin) peptide remains associated with pMSP3535 and pMSP3535VA. Unique restriction endonuclease cleavage sites are also shown. This erratum is doi: 10.1006/plas.2001.1513. 61
0147-619X/01 $35.00 Copyright © 2001 by Academic Press All rights of reproduction in any form reserved.
ERRATUM Volume 44, Number 2 (2000), in the short communication “Improved Vectors for Nisin-Controlled Expression in Gram-Positive Bacteria,” by Edward M. Bryan, Taeok Bae, Michiel Kleerebezem, and Gary M. Dunny, pages 183–190 (doi: 10.1006/plas.2000.1484): On page 186, Fig. 1 contains an incorrect size and some restriction cleavage sites that do not actually exist in two of the vectors. For the reader’s convenience, the correct figure with its legend is printed here.
FIG.1. Plasmid construction. (A) Maps and construction scheme for pMSP3535. Black arrows represent antibiotic resistance markers and gram-positive bacterial replication genes. E. coli genes are shown as boxed arrows, and nisin-associated genes and promoters are shown as gray arrows. Selected restriction enzyme cleavage sites are shown. A map of pMSP3535VA is also shown, but its construction scheme is not shown (see text). (B) Selected portions of the promoter region and multicloning site are shown for pMSP3535 and pMSP3535VA, as well as for pMSP3545. The putative –10 promoter regions are TACAAT. C represents the transcriptional start site. ATG is the translational start site of nisA, Nucleotide sequence encoding the signal sequence and one-third of the mature NisA (nisin) peptide remains associated with pMSP3535 and pMSP3535VA. Unique restriction endonuclease cleavage sites are also shown. This erratum is doi: 10.1006/plas.2001.1513. 61
0147-619X/01 $35.00 Copyright © 2001 by Academic Press All rights of reproduction in any form reserved.