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In vitro immune absorption of antisperm antibodies with immunobead-rise, immunomagnetic, and immunocolumn separation techniques
330
Citations from the literature
The diagnosis of IHH was based on clinical and laboratory features and testicular biopsy specimen study and was further supported by results of stimulation tests and gonadotropinreleasing hormone (GnRH) test. Two treatment modalities were used: subcutaneous injections of 500 mug LH-RH twice daily; and perpetual subcutaneous injection, via portable infusion pump, of 25 ng/kg LH-RH, at 90-minute intervals. Two patients required a short second period of pulsatile treatment to cause a second pregnancy of their spouses. The pump proved to yield better results, compared with intermittent injections, in respect to endocrine responses, spermatogenesis, and fertility capacity. Normal levels of luteinizing hormone and follicle-stimulating hormone were reached in 2 to 3 weeks and normal testosterone levels in 8 to 10 weeks from the start of treatment. Sperm counts rose to > 60 x l@/ml viable spermatozoa with < 15% of abnormal forms in 3 to 5 months, and the wives conceived. Of a total of 18 deliveries of healthy infants, 12 offspring were identified genetically with their fathers. Four women were still pregnant at the conclusion of the study. The pump was well tolerated, without special operational problems to the patients. Pulsatile treatment is therefore recommended in the treatment of welldiagnosed and carefully selected cases of incomplete IHH.
Right varicocelectomy in selected infertile patients who have failed to improve after previous left varicocelectomy Amelar RD; Dubin L
Department of Urology, New York University Medical Center, New York, NY 10016, USA FERTIL. STERIL.; 47/5 (833-837) 1987 In the past 8 years we found 41 infertile men with poor semen quality in whom an overlooked right varicocele might have been the reason for failure to improve after previous left varicocelectomy. None had ever fathered a child. After we performed sequential right varicocelectomy in these 41 patients, semen quality improved in 23 (56%). Twenty-seven pregnancies have been achieved to date by the wives of 18 (43%) of the patients whose semen improved. No pregnancies were caused by those whose semen failed to improve. Infertile men should be carefully examined for varicoceles on both sides, and bilateral varicocelectomy should be performed when indicated. Selected infertile patients who have failed to improve after left varicocelectomy and are found subsequently to have a right varicocele may yet improve following correction of the right varicocele. The use of sperm penetration assay in evaluation of men with varicocele Plymate SR; Nagao RR; Muller CH; Paulsen CA
Department of Internal Medicine, University of Washington, Seattle, WA, USA In vitro immune absorption of antisperm antibodies with immunobead-rise, immunomagnetic, and immunocolumn separation techniques Kiser CC; Alexander NJ; Fuchs EF; Fulgham DL Oregon Health Sciences University, Portland, OR 97201, USA FERTIL. STERIL.; 47/3 (466-474) 1987 Fourteen men with a mean duration of infertility > 3 years who had significant sperm immobilizing or spermagglutinating antibodies were studied. All patients had < 20%. IgG or IgA immunobinding to sperm in their seminal plasma and 7 had immunobinding levels of > 50%. Sperm from these men were less able to penetrate an overlaying buffer layer than sperm from a fertile control. Addition of immunobeads to the specimen was of little use, because few motile sperm could swim into the overlaying buffer; retained immunobeads were noted in the buffer layer of 18-hour capacitated specimens. Magnetic isolation of antibody-coated sperm from antibodyfree sperm avoids potential damage to fragile sperm through centrifugation. Viable spermatozoa were isolated from magnetite-complexed spermatozoa, but the motility of the isolated spermatozoa deteriorated rapidly during the subsequent capacitation period. Passage of diluted ejaculate through a column of dextran beads for antisperm antibody processing (ASAP) was associated with superior sperm quality and fertilizing potential. The use of ASAP resulted in good sperm velocity and linearity and improved sperm function, as measured with the hamster egg penetration test. Sperm from men with immunologically mediated infertility can be used for artificial processed through the ASAP and insemination of their partners or in an in vitro fertilization program.
Int J Gynecol Obstet 26
FERTIL. STERIL.; 47/4 (680-683) 1987 In order to study the ability of the sperm penetration assay (SPA) to correctly classify the fertility status of men, we prospectively examined the results of the SPA performed on the semen of three groups of men of known fertility status. The groups included 67 normal men without varicoceles whose wives were pregnant (VARN), 51 men with a palpable varicocele whose wives were pregnant (VARF), and 30 infertile men with varicoceles (VARI). Two SPAS were done on each subject. Ninety-seven percent of the VARls showed < 15% penetration on a single test, and 91% showed < 15% on both tests. On a single test 61% of the VARNs and 68% of the VARFs were < 15%. If 0 penetration were used as the criteria of infertility, then 40% of the VARIs, 27% of the VARFs, and 12% of the VARNs would be classified as being infertile. These data suggest that the SPA cannot independently define male fertility status and should be used in conjunction with the standard semen analysis and clinical evaluation of the couple to assess male fertility potential. Pharmacologic induction of multiple follicular development improves the success rate of artificial insemination with husband’s semen in couples with male-related or unexplained infertility Melis GB; Paoletti AM; Strigini F; et al
Clinica Ostetrica e Ginecologica, Universita degli Studi di Pisa, 56100 Piss, Italy FERTIL. STERIL.; 47/3 (441-445) 1987 The use of artificial insemination with (AIH) as treatment for couples with unexplained infertility is often disappointing.
husband’s semen male-related or The aim of the
Citations from the literature
The diagnosis of IHH was based on clinical and laboratory features and testicular biopsy specimen study and was further supported by results of stimulation tests and gonadotropinreleasing hormone (GnRH) test. Two treatment modalities were used: subcutaneous injections of 500 mug LH-RH twice daily; and perpetual subcutaneous injection, via portable infusion pump, of 25 ng/kg LH-RH, at 90-minute intervals. Two patients required a short second period of pulsatile treatment to cause a second pregnancy of their spouses. The pump proved to yield better results, compared with intermittent injections, in respect to endocrine responses, spermatogenesis, and fertility capacity. Normal levels of luteinizing hormone and follicle-stimulating hormone were reached in 2 to 3 weeks and normal testosterone levels in 8 to 10 weeks from the start of treatment. Sperm counts rose to > 60 x l@/ml viable spermatozoa with < 15% of abnormal forms in 3 to 5 months, and the wives conceived. Of a total of 18 deliveries of healthy infants, 12 offspring were identified genetically with their fathers. Four women were still pregnant at the conclusion of the study. The pump was well tolerated, without special operational problems to the patients. Pulsatile treatment is therefore recommended in the treatment of welldiagnosed and carefully selected cases of incomplete IHH.
Right varicocelectomy in selected infertile patients who have failed to improve after previous left varicocelectomy Amelar RD; Dubin L
Department of Urology, New York University Medical Center, New York, NY 10016, USA FERTIL. STERIL.; 47/5 (833-837) 1987 In the past 8 years we found 41 infertile men with poor semen quality in whom an overlooked right varicocele might have been the reason for failure to improve after previous left varicocelectomy. None had ever fathered a child. After we performed sequential right varicocelectomy in these 41 patients, semen quality improved in 23 (56%). Twenty-seven pregnancies have been achieved to date by the wives of 18 (43%) of the patients whose semen improved. No pregnancies were caused by those whose semen failed to improve. Infertile men should be carefully examined for varicoceles on both sides, and bilateral varicocelectomy should be performed when indicated. Selected infertile patients who have failed to improve after left varicocelectomy and are found subsequently to have a right varicocele may yet improve following correction of the right varicocele. The use of sperm penetration assay in evaluation of men with varicocele Plymate SR; Nagao RR; Muller CH; Paulsen CA
Department of Internal Medicine, University of Washington, Seattle, WA, USA In vitro immune absorption of antisperm antibodies with immunobead-rise, immunomagnetic, and immunocolumn separation techniques Kiser CC; Alexander NJ; Fuchs EF; Fulgham DL Oregon Health Sciences University, Portland, OR 97201, USA FERTIL. STERIL.; 47/3 (466-474) 1987 Fourteen men with a mean duration of infertility > 3 years who had significant sperm immobilizing or spermagglutinating antibodies were studied. All patients had < 20%. IgG or IgA immunobinding to sperm in their seminal plasma and 7 had immunobinding levels of > 50%. Sperm from these men were less able to penetrate an overlaying buffer layer than sperm from a fertile control. Addition of immunobeads to the specimen was of little use, because few motile sperm could swim into the overlaying buffer; retained immunobeads were noted in the buffer layer of 18-hour capacitated specimens. Magnetic isolation of antibody-coated sperm from antibodyfree sperm avoids potential damage to fragile sperm through centrifugation. Viable spermatozoa were isolated from magnetite-complexed spermatozoa, but the motility of the isolated spermatozoa deteriorated rapidly during the subsequent capacitation period. Passage of diluted ejaculate through a column of dextran beads for antisperm antibody processing (ASAP) was associated with superior sperm quality and fertilizing potential. The use of ASAP resulted in good sperm velocity and linearity and improved sperm function, as measured with the hamster egg penetration test. Sperm from men with immunologically mediated infertility can be used for artificial processed through the ASAP and insemination of their partners or in an in vitro fertilization program.
Int J Gynecol Obstet 26
FERTIL. STERIL.; 47/4 (680-683) 1987 In order to study the ability of the sperm penetration assay (SPA) to correctly classify the fertility status of men, we prospectively examined the results of the SPA performed on the semen of three groups of men of known fertility status. The groups included 67 normal men without varicoceles whose wives were pregnant (VARN), 51 men with a palpable varicocele whose wives were pregnant (VARF), and 30 infertile men with varicoceles (VARI). Two SPAS were done on each subject. Ninety-seven percent of the VARls showed < 15% penetration on a single test, and 91% showed < 15% on both tests. On a single test 61% of the VARNs and 68% of the VARFs were < 15%. If 0 penetration were used as the criteria of infertility, then 40% of the VARIs, 27% of the VARFs, and 12% of the VARNs would be classified as being infertile. These data suggest that the SPA cannot independently define male fertility status and should be used in conjunction with the standard semen analysis and clinical evaluation of the couple to assess male fertility potential. Pharmacologic induction of multiple follicular development improves the success rate of artificial insemination with husband’s semen in couples with male-related or unexplained infertility Melis GB; Paoletti AM; Strigini F; et al
Clinica Ostetrica e Ginecologica, Universita degli Studi di Pisa, 56100 Piss, Italy FERTIL. STERIL.; 47/3 (441-445) 1987 The use of artificial insemination with (AIH) as treatment for couples with unexplained infertility is often disappointing.
husband’s semen male-related or The aim of the