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In vitro interaction of human immunodeficiency virus type 1 (HIV-1) with human trophoblast and malignant trophoblastic cell lines
Abstratts
447
IN VITRO INTERACTION OF HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 (HIV-l) WITH HUMAN TROPHOBLAST AND MALIGNANT TROPHOBLASTIC CELL LINES V. Zachar’, G. Aboagye-Mathiesen, N. Norskov-Lauritsenb, C. Juhlb & P. Ebbesen (” Institute ofVirology, Slovak Academy of Sciences, 809 39 Bratislava 9, Czechoslovakia and hThe Danish Cancer Society, Department of Virus and Cancer, DK-8000 Aarhus, Denmark) The primary cultures of immunopurified human term trophoblast and choriocarcinoma cell lines BeWo, JAR and JEG were inoculated with three biologically distinct HIV- 1 strains (RF, 3B, and NDK). Each of the cell cultures became infected with at least one viral isolate that was documented by the presence of intracellular viral DNA using PCR, by the rescue of infectious virus after cocultivation with the permissive lymphoid cells CEM-SS or by the in situ expression of virus-specific antigens. The target cells exhibited a particular pattern of susceptibility to infection which in general was highest with RF strain. The analysis of HIV principal receptor CD4 at the transcriptional level and as a surface antigen indicated, however, that this molecule was not the factor determining viral penetration.
447
IN VITRO INTERACTION OF HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 (HIV-l) WITH HUMAN TROPHOBLAST AND MALIGNANT TROPHOBLASTIC CELL LINES V. Zachar’, G. Aboagye-Mathiesen, N. Norskov-Lauritsenb, C. Juhlb & P. Ebbesen (” Institute ofVirology, Slovak Academy of Sciences, 809 39 Bratislava 9, Czechoslovakia and hThe Danish Cancer Society, Department of Virus and Cancer, DK-8000 Aarhus, Denmark) The primary cultures of immunopurified human term trophoblast and choriocarcinoma cell lines BeWo, JAR and JEG were inoculated with three biologically distinct HIV- 1 strains (RF, 3B, and NDK). Each of the cell cultures became infected with at least one viral isolate that was documented by the presence of intracellular viral DNA using PCR, by the rescue of infectious virus after cocultivation with the permissive lymphoid cells CEM-SS or by the in situ expression of virus-specific antigens. The target cells exhibited a particular pattern of susceptibility to infection which in general was highest with RF strain. The analysis of HIV principal receptor CD4 at the transcriptional level and as a surface antigen indicated, however, that this molecule was not the factor determining viral penetration.