In vitro studies of the influenza virus replicase

HOST CELL PROTEINS

SUSAN R. CARLSEN University School The involvement Sendai

virus

reactions.

of host cell proteins

Soluble

soluble

(elongation

virus

nucleocapsid detergent from

capsids

under

synthesis cell

template. Studies cellular

proteins

from uninfected

but

actin,

Sendai

that

actin

monoclonal

STUDIES

cells

OF THE

also

insufficient

to

Cellular with

replication nucleoside

from

with

HAY, National Hill, London

with

molecular

When

triphosphates, virus during

against

Sendai

replication

synthesized

in vitro.

nucleo-

total a 43K

RNA host

nucleocapsid replication.

actin and other

will be discussed.

REPLICASE

Institute NW7 IAA,

capable of synthesizing both A-cRNA from influenza virus infected cells. will be discussed. 25

intracellular

proteins

directed

VIRUS

RNA repli-

initiate

newly

nucleocapsids

INFLUENZA

supports

the Sendai

RNA synthesis

K.W. TIBBLES and A.J. Medical Research, Mill

Cytoplasmic extracts have been obtained of these activities

Sendai virus.

antibodies

on _in vitro Sendai

--IN VITRO

to be required

associated

is eliminated

of

synthesis

found

associated

allow

RNA

from detergent-disrupted

virus.

by withholding is found

on the effect

cells were

Vanderbilt

and replication

in vitro --

from pre-initiated is

Kd are tightly

conditions

through

from uninfected

templates,

disrupted

proteins

48

in the transcription

examined

and encapsidation)

is inhibited

The

been

fraction

50-60

protein,

has

RNA and mRNA synthesis

cation

from

RNA

protein

weights

IN SENDAI VIRUS RNA SYNTHESIS

AND SUE A. MOYER, Department of Microbiology, of Medicine, Nashville, TN 3723'2, U.S.A.

genome

for both leader The

UTILIZED

and

49

for England

vRNA in

CharacterKtr

vitro