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Incidence of Tumours in Sheep in Spain
J. Comp. Path. 2016, Vol. 154, 58e123
ESVP, ECVP and NSVP Proceedings 2015
TYROSINE KINASE RECEPTOR EXPRESSION IN CANINE LIPOSARCOMA G. Avallone *, V. Pellegrino *, P. Roccabianca y, E. Lepri z, L. Crippa x, G. Beha *, L. De Tolla k and G. Sarli* *Department of Veterinary Medical Sciences, University of Bologna, y Dipartimento di Scienze Veterinarie e Sanita Pubblica, University of Milano, z Dipartimento di Medicina Veterinaria, University of Perugia, xIstovet, Besana Brianza (MB), Italy and kUniversity of Maryland, School of Medicine, Baltimore, MD, USA Introduction: Canine liposarcoma is an uncommon, locally invasive, soft tissue sarcoma (STS) for which wide resection is the main treatment. Tyrosine kinase receptors (TKRs) are considered putative targets for adjunctive therapies of some human STS to reduce recurrence rate. The aim of this study was to evaluate FGF2, PDGF-b and TKR expression by immunohistochemistry in canine liposarcoma to define their putative role as therapeutic targets. Materials and Methods: Selected cases were reviewed histologically. Mitotic index (MI), grade and MIB-1-based proliferation index (PI) were assessed. Immunohistochemical expression of FGF2, FGFR1, PDGF-b, and PDGFR-a was classified as high (70% of positive neoplastic cells) or low (0e70% of neoplastic cells) according to a previously reported scoring method. Sections of granulation tissue were used as positive controls. The Spearman test was used to identify possible correlations between FGF2, FGFR1, PDGF-b, and PDGFR-a expression with grade, MI and PI. Results: Fifty liposarcomas were collected. Median MI was 5 (range 1e56); median PI was 14.9% (range 1.1e76.8%). FGF2 was highly expressed in 31 cases (62%), FGFR1 in 34 cases (68%), PDGF-b in 40 cases (81.6%) and PDGFR-a in 34 cases (70.8%). MI negatively correlated with FGF2 (R 5 -0.41; P ! 0.01) and positively correlated with PDGFR-a (R 5 0.33; P ! 0.01). MI was lower in FGF2 high cases and higher in PDGFR-a high cases. Conclusions: These results suggests that while the FGF2-mediated pathway may have a minor role in liposarcoma cell growth, PDGFR-a is directly involved in canine liposarcoma cell proliferation, representing a promising target for anti-TKR therapy.
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INCIDENCE OF TUMOURS IN SHEEP IN SPAIN C. P erez-Martınez *, G. Domınguez y, J.F. Garcıa Marın *, E. Gayo * and M.J. Garcıa-Iglesias* *Pathological Anatomy Section, Animal Health Department, Veterinary School, University of Leon, Leon and yINCARSA Slaughterhouse, Burgos, Spain Introduction: Tumours have been diagnosed with low incidence in sheep, but it is important to recognize this pathology and make a differential diagnosis, especially for veterinary clinicians and meat inspectors. The aim of this study was to determine the incidence and type of neoplasia in sheep in Spain. Materials and Methods: A total of 677,553 sheep were inspected in the slaughterhouse over a period of 5 years. The number of lambs under 3 months old was 645,867 and there were 31,686 adult sheep, all from northern Spain. Histopathological studies were carried out in any suspicious tumour lesion. Results: Thirty-six (0.005%) cases of neoplasia were diagnosed, 34 in adult animals (0.1%) and two in lambs. Most (n 5 18) were intestinal adenocarcinomas, always found in adult sheep, and all were from different flocks. In lambs, blastomas were the only tumours observed. Thirteen types of tumours were described. Conclusions: The high incidence of intestinal adenocarcinoma is similar to that described in Australia, compared with the sporadic cases and few reports from other countries.
ESVP, ECVP and NSVP Proceedings 2015
TYROSINE KINASE RECEPTOR EXPRESSION IN CANINE LIPOSARCOMA G. Avallone *, V. Pellegrino *, P. Roccabianca y, E. Lepri z, L. Crippa x, G. Beha *, L. De Tolla k and G. Sarli* *Department of Veterinary Medical Sciences, University of Bologna, y Dipartimento di Scienze Veterinarie e Sanita Pubblica, University of Milano, z Dipartimento di Medicina Veterinaria, University of Perugia, xIstovet, Besana Brianza (MB), Italy and kUniversity of Maryland, School of Medicine, Baltimore, MD, USA Introduction: Canine liposarcoma is an uncommon, locally invasive, soft tissue sarcoma (STS) for which wide resection is the main treatment. Tyrosine kinase receptors (TKRs) are considered putative targets for adjunctive therapies of some human STS to reduce recurrence rate. The aim of this study was to evaluate FGF2, PDGF-b and TKR expression by immunohistochemistry in canine liposarcoma to define their putative role as therapeutic targets. Materials and Methods: Selected cases were reviewed histologically. Mitotic index (MI), grade and MIB-1-based proliferation index (PI) were assessed. Immunohistochemical expression of FGF2, FGFR1, PDGF-b, and PDGFR-a was classified as high (70% of positive neoplastic cells) or low (0e70% of neoplastic cells) according to a previously reported scoring method. Sections of granulation tissue were used as positive controls. The Spearman test was used to identify possible correlations between FGF2, FGFR1, PDGF-b, and PDGFR-a expression with grade, MI and PI. Results: Fifty liposarcomas were collected. Median MI was 5 (range 1e56); median PI was 14.9% (range 1.1e76.8%). FGF2 was highly expressed in 31 cases (62%), FGFR1 in 34 cases (68%), PDGF-b in 40 cases (81.6%) and PDGFR-a in 34 cases (70.8%). MI negatively correlated with FGF2 (R 5 -0.41; P ! 0.01) and positively correlated with PDGFR-a (R 5 0.33; P ! 0.01). MI was lower in FGF2 high cases and higher in PDGFR-a high cases. Conclusions: These results suggests that while the FGF2-mediated pathway may have a minor role in liposarcoma cell growth, PDGFR-a is directly involved in canine liposarcoma cell proliferation, representing a promising target for anti-TKR therapy.
111
INCIDENCE OF TUMOURS IN SHEEP IN SPAIN C. P erez-Martınez *, G. Domınguez y, J.F. Garcıa Marın *, E. Gayo * and M.J. Garcıa-Iglesias* *Pathological Anatomy Section, Animal Health Department, Veterinary School, University of Leon, Leon and yINCARSA Slaughterhouse, Burgos, Spain Introduction: Tumours have been diagnosed with low incidence in sheep, but it is important to recognize this pathology and make a differential diagnosis, especially for veterinary clinicians and meat inspectors. The aim of this study was to determine the incidence and type of neoplasia in sheep in Spain. Materials and Methods: A total of 677,553 sheep were inspected in the slaughterhouse over a period of 5 years. The number of lambs under 3 months old was 645,867 and there were 31,686 adult sheep, all from northern Spain. Histopathological studies were carried out in any suspicious tumour lesion. Results: Thirty-six (0.005%) cases of neoplasia were diagnosed, 34 in adult animals (0.1%) and two in lambs. Most (n 5 18) were intestinal adenocarcinomas, always found in adult sheep, and all were from different flocks. In lambs, blastomas were the only tumours observed. Thirteen types of tumours were described. Conclusions: The high incidence of intestinal adenocarcinoma is similar to that described in Australia, compared with the sporadic cases and few reports from other countries.