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Increased dietary protein increases the capacity of urea synthesis in rats
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CLINICAL VALUE OF SERUM HDV-RNA IN ACUTE AND CHRONIC DELTA INFECTION. R. ESTEBAN, M. BUTI, J. FERNANDEZ-LARREA, R. JARDI, J.I. ESTEBAN, J. GUARDIA. Liver Diseases Section. Departament of Internal Medicine. Hospital Vall dHebrd Universidad Autonoma. Barcelona. Spain.
To a s s e s s t h e c l i n i c a l significance and d l a g n o s t * c utility o f serum hepatitis D v i r u s RNA, s e r a f r o m 52 p a t , e n t s , most o f them d r u g a b u s e r s , w i t h a c u t e HDU l n f e c t * n (26 coinfectlons and 16 s u p e r l n f e c t l o n s ) (Group I ) and 26 w i t h c h r o n i c h e p a t i t i s D (group II) were investigated f o r t h e p r e s e n c e o f HDU-RNA by s p o t h y b r i d i z a t i o n assay. Overall, HDV-RNA was detected in 64% o f a c u t e c a s e s d u r i n g t h e f i r s t week o£ symptoms, w i t h no significant differences betweeen coinfections and s u ~ e r i n f e c t i o n s , and in 62~ o f the chronically infected patients. I n g r o u p I , t h e p r e s e n c e o f serum HDV-RNA showed t o be transient ( l e s s t h a n 4 w e e k s ) and p a r a l l e l e d t h a t o f serum HD-Ag in t h o s e 0 a t i e n t s with HBU-HOU coinfection, w h e r e a s in t h e ma3ority of those with HDU s u p e r i n f e c t i o n HDU-RNA was p e r s i s t e n t l y detected in a s t a b l e (8 c a s e s ) o r f l u c t u a t i n g pattern (5 c a s e s ) during the six months o f follow-up.Among patients in group I I and those in Group I who progressed to chronic*tv, the 0resence o f serum HDU-RNA was a s s o c i a t e d with severe hepatic lession and c o r r e l a t e d w i t h t h e p r e s e n c e o f HD-Ag ( 7 9 ~ ) within the liver cells, and t h a t o f serum I g M a n t i - H D . A substantial proportion o£ p a t i e n t s (21~) with chronic HDV i n f e c t i o n were simultaneously positive f o r HDV-RNA and HBV-DNA, t h u s s u g g e s t i n g that a t l e a s t among d r u g a b u s e r s w i t h c h r o n i c h e p a t i t i s D b o t h v i r u s e s may r e p l i c a t e at the same time. Our r e s u l t s suggest that serum HDV-RNA is a good marker for chronic HDU i n f e c t i o n which may be useful t o p r e d l c t the outcome of acute HDU i n f e c t i o n and to monltor the activity of the chronic disease.
42
Ik~REAS~9 DIETARY P~0TEIN INCREASES THE CAPACITY OF UREA SYNTHF.qIS IN RATS. K.Falk Petersen, Department of Hepatology A-2152, Rigshospitalet, Blegdamsvej 3, Co~9_nhagen DK-2100, Denmark.
• Earlier experiments have suggested a r ~ a t o r y role of diet protein on the urea synthesis. Tne capacity of urea nitrogen synthesis was exmined i~ 200 g female rats during_ 14 days with a normal diet of 17% protein or with diets containing 9% or 51% protein. The rats were nephrectomized and alanlne was given as a prime-continuous infusion of 0.02 ~ o i per minute for 100 minutes to obtain steady state concentrations of alpha-amino-nitrogen between 7.5 and 12.0 mmol/l, where the urea synthesis is at ~ and independent of substrate concentration. The capacity of urea nitrogen synthesis was calculated as dc/dt. 0.63.~R.I.25, where dc/dt is the slope of the linear regression of urea blood concentration on t/me, 0.63.~q the urea volume of distribution, and 1.25 the correction factor for intestinal hydrolysis. The capacity of urea synthesis was 7.4~mDl/(min-100g BW) in the control rats, and 6.5}~TO1 per minute per 100g BW with 9% dietary protein. In the group fed 51% protein the capacity of urea nitrogen synthesis was 18.6 )xnol/(min.100g BW). In conclusion: urea synthesis shows adaptive changes to variation in dietary protein, one of the factors re cnllating urea synthesis is suggestively the activity of urea cycle enzymes.
$23
CLINICAL VALUE OF SERUM HDV-RNA IN ACUTE AND CHRONIC DELTA INFECTION. R. ESTEBAN, M. BUTI, J. FERNANDEZ-LARREA, R. JARDI, J.I. ESTEBAN, J. GUARDIA. Liver Diseases Section. Departament of Internal Medicine. Hospital Vall dHebrd Universidad Autonoma. Barcelona. Spain.
To a s s e s s t h e c l i n i c a l significance and d l a g n o s t * c utility o f serum hepatitis D v i r u s RNA, s e r a f r o m 52 p a t , e n t s , most o f them d r u g a b u s e r s , w i t h a c u t e HDU l n f e c t * n (26 coinfectlons and 16 s u p e r l n f e c t l o n s ) (Group I ) and 26 w i t h c h r o n i c h e p a t i t i s D (group II) were investigated f o r t h e p r e s e n c e o f HDU-RNA by s p o t h y b r i d i z a t i o n assay. Overall, HDV-RNA was detected in 64% o f a c u t e c a s e s d u r i n g t h e f i r s t week o£ symptoms, w i t h no significant differences betweeen coinfections and s u ~ e r i n f e c t i o n s , and in 62~ o f the chronically infected patients. I n g r o u p I , t h e p r e s e n c e o f serum HDV-RNA showed t o be transient ( l e s s t h a n 4 w e e k s ) and p a r a l l e l e d t h a t o f serum HD-Ag in t h o s e 0 a t i e n t s with HBU-HOU coinfection, w h e r e a s in t h e ma3ority of those with HDU s u p e r i n f e c t i o n HDU-RNA was p e r s i s t e n t l y detected in a s t a b l e (8 c a s e s ) o r f l u c t u a t i n g pattern (5 c a s e s ) during the six months o f follow-up.Among patients in group I I and those in Group I who progressed to chronic*tv, the 0resence o f serum HDU-RNA was a s s o c i a t e d with severe hepatic lession and c o r r e l a t e d w i t h t h e p r e s e n c e o f HD-Ag ( 7 9 ~ ) within the liver cells, and t h a t o f serum I g M a n t i - H D . A substantial proportion o£ p a t i e n t s (21~) with chronic HDV i n f e c t i o n were simultaneously positive f o r HDV-RNA and HBV-DNA, t h u s s u g g e s t i n g that a t l e a s t among d r u g a b u s e r s w i t h c h r o n i c h e p a t i t i s D b o t h v i r u s e s may r e p l i c a t e at the same time. Our r e s u l t s suggest that serum HDV-RNA is a good marker for chronic HDU i n f e c t i o n which may be useful t o p r e d l c t the outcome of acute HDU i n f e c t i o n and to monltor the activity of the chronic disease.
42
Ik~REAS~9 DIETARY P~0TEIN INCREASES THE CAPACITY OF UREA SYNTHF.qIS IN RATS. K.Falk Petersen, Department of Hepatology A-2152, Rigshospitalet, Blegdamsvej 3, Co~9_nhagen DK-2100, Denmark.
• Earlier experiments have suggested a r ~ a t o r y role of diet protein on the urea synthesis. Tne capacity of urea nitrogen synthesis was exmined i~ 200 g female rats during_ 14 days with a normal diet of 17% protein or with diets containing 9% or 51% protein. The rats were nephrectomized and alanlne was given as a prime-continuous infusion of 0.02 ~ o i per minute for 100 minutes to obtain steady state concentrations of alpha-amino-nitrogen between 7.5 and 12.0 mmol/l, where the urea synthesis is at ~ and independent of substrate concentration. The capacity of urea nitrogen synthesis was calculated as dc/dt. 0.63.~R.I.25, where dc/dt is the slope of the linear regression of urea blood concentration on t/me, 0.63.~q the urea volume of distribution, and 1.25 the correction factor for intestinal hydrolysis. The capacity of urea synthesis was 7.4~mDl/(min-100g BW) in the control rats, and 6.5}~TO1 per minute per 100g BW with 9% dietary protein. In the group fed 51% protein the capacity of urea nitrogen synthesis was 18.6 )xnol/(min.100g BW). In conclusion: urea synthesis shows adaptive changes to variation in dietary protein, one of the factors re cnllating urea synthesis is suggestively the activity of urea cycle enzymes.
$23