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Increased Expression of ID2, PRELP, and SMOC2 Genes in Patients with Endometriosis
Abstracts / Journal of Minimally Invasive Gynecology 23 (2016) S1–S252 common. hysteroscopic examination revealed pathology in 45 patients and endometrial polyp as most common finding. biopsy confirmred pathology in 50 patients with hyperplasia as most common report. considering biopsy as gold standard transabdominal ultrasonography was 73.03% sensitive 95.83% specific with a positive and negative predictive vaue of 95% and 96.6%, for hysteroscopy sensitivity 89.79%, specificity 97.95%with a positive and negative predictive value of 97.77% and 90.56%. Limitation: Non-availability of transvaginal ultrasound. Conclusion: Ultrasonography is a non invasive , widely availble investigative modality and along with endometrial also comment on adnexal pathologies. hysteroscopy is diagnostic as well as therapeutic also but still in developing countries universal availability is an isssue. So its the availability and patients symptoms,age and clinical findings finally guides us the investigative modality to be used. Virtual Posters – Endometriosis
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the proliferative phase of their menstrual cycle and the eutopic and ectopic endometrium of women with endometriosis in the proliferative phase. Paired tissue samples from 20 women were analyzed: 10 from endometrial and peritoneal endometriotic lesions and 10 from endometrial and ovarian endometriotic lesions. As controls, 16 endometrium samples were collected from women without endometriosis. The expression analysis was performed by real-time Polymerase Chain Reaction. Measurements and Main Results: There was no significant difference between gene expression in the endometrium of women with and without endometriosis. The ID2 gene was expressed in the most advanced stage of endometriosis and in ovarian endometriomas, the PRELP was expressed in peritoneal lesions, and the SMOC2 was expressed in both peritoneal and endometrioma lesions. Conclusion: Considering that the genes studied participate either directly or indirectly in cellular processes that can lead to cell migration, angiogenesis, and inappropriate invasion, it is possible that the dysregulation of these genes causes the development and maintenance of ectopic tissue.
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Influence of Antiangiogenic Agent Bevacizumab on Endometriosis Experimentally Induced in Rats Rosa-e-Silva JC, Zani ACT, Valerio FP, Meola J, Poli-Neto OB, Candidodos-Reis FJ, Nogueira AA. Gynecology and Obstetrics, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil
Influence of Antiangiogenic Agent Propranolol on Endometriosis Experimentally Induced in Rats Rosa-e-Silva JC, Zanardi JVC, Fortunato GG, Meola J, Poli-Neto OB, Candido-dos-Reis FJ, Nogueira AA. Gynecology and Obstetrics, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil
Study Objective: The aim of this study is to evaluate the effect of antiangiogenic agent bevacizumab in two different dosages on endometriotic lesions induced in rats. Design: Experimental study. Setting: This study was carried out in the Department of Obstetrics and Gynecology and Experimental Surgery Sector, in the School of Medicine of Ribeir~ao Preto, University of S~ao Paulo. Patients: 30 female Wistar rats. Intervention: Endometriotic lesions was first induced in the 30 rats by the implantation of endometrium tissue on the peritoneum. The rats were, then, allocated in 3 groups of 10 rats. The first was the control group, in which no medication was used after surgery. The second received the drug twice a month and the third received the same drug (bevacizumab) twice a week. Measurements and Main Results: At the time of removal of the lesion, it was observed that the control group developed typical endometriotic lesions, while both medication groups did not present them. All the lesions and a piece of uterine tissue were removed of all the 30 rats for further genetic and pathologic evaluation of the angiogenic genes and receptors expression. Conclusion: The endometriotic lesions are richly vascularized and many studies have proven that the emergence of new vessels is central. This study showed that there is an important difference between the appearance of the lesions when using or not antiangiogenic medication. Further evaluation will prove theses differences on molecular levels.
Study Objective: To assess the anti-angiogenic effect of two doses of propranolol on the endometriosis lesions induced in female rats compared to controls. Design: Experimental study. Setting: Experimental Surgery Sector. Patients: 30 female Wistar rats. Intervention: We studied the effect of this drug on differentiation markers, invasion, cell proliferation and apoptosis by immunohistochemical staining, and also the influence on the adhesion, motility and angiogenesis of endometriotic lesions by extracting total RNA, cDNA synthesis and quantification by real time PCR. We used 30 Wistar adult rats, virgin females and submitted to laparotomy for induction of endometriosis lesions. The rats were listed in three groups, and sacrificed after 14 days of low dose treatment (PB,n=10) and high dose (PA,n=10) of propranolol; and control group (C,n=10) without treatment. The lesions were excised for histological analysis with the contralateral uterine horn and confirmed the presence of endometrial glandular and stromal tissue. Immunohistochemical reactions were performed for MMP-9, Metallothionein, TIMP-2, Caspase-8 and PCNA, and evaluation of gene expression by VEGF, CALD1 PCNA, TNF and SPARC primes, in tissue from injury and uterus. Measurements and Main Results: In this study, for the material obtained from lesions, was observed a reduction in immunohistochemical staining for MMP-9 in the control group (100% positive) to the treated groups - PA 60% of positivity and PB = 11.1% positive; p\0.05. Moreover, for metallothionein, with 60% of positivity in the control group, 10% and 11.1% for PA, PB, p\0.05; the quantification by real-time PCR, to the significance level of 0.05, there was a decrease in gene expression of PCNA (median control group=1.04; PA =0.32; PB=0.69). In other immunohistochemistry markers and quantification of gene expression to the material from injury no significant differences were observed. Conclusion: Treatment with anti-angiogenic drugs offers new prospects for therapeutic approach for patients with endometriosis.
589 Increased Expression of ID2, PRELP, and SMOC2 Genes in Patients with Endometriosis Rosa-e-Silva JC, Ara ujo FM, Meola J, Ferriani RA, Nogueira AA. Gynecology and Obstetrics, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil Study Objective: Endometriosis is a benign, estrogen-dependent disease with symptoms such as pelvic pain and infertility, and it is characterized by the ectopic distribution of endometrial tissue. This study aimed to analyze the gene expression ID2, PRELP and SMOC2 in women with and without endometriosis. Design: Cross-sectional study. Setting: A tertiary referral hospital. Patients: Women with or without endometriosis. Intervention: The expression of the ID2, PRELP, and SMOC2 genes was compared between the endometrium of women without endometriosis in
591 Spontaneous Fertility After Expectant or Surgical Management of Rectovaginal Endometriosis with/ without Ovarian Endometrioma: A 7-Year Retrospective Analysis Leone Roberti Maggiore U, Scala C, Tafi E, Racca A, Venturini PL, Ferrero S. Department of Neurosciences, Rehabilitation, Ophthalmology,
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the proliferative phase of their menstrual cycle and the eutopic and ectopic endometrium of women with endometriosis in the proliferative phase. Paired tissue samples from 20 women were analyzed: 10 from endometrial and peritoneal endometriotic lesions and 10 from endometrial and ovarian endometriotic lesions. As controls, 16 endometrium samples were collected from women without endometriosis. The expression analysis was performed by real-time Polymerase Chain Reaction. Measurements and Main Results: There was no significant difference between gene expression in the endometrium of women with and without endometriosis. The ID2 gene was expressed in the most advanced stage of endometriosis and in ovarian endometriomas, the PRELP was expressed in peritoneal lesions, and the SMOC2 was expressed in both peritoneal and endometrioma lesions. Conclusion: Considering that the genes studied participate either directly or indirectly in cellular processes that can lead to cell migration, angiogenesis, and inappropriate invasion, it is possible that the dysregulation of these genes causes the development and maintenance of ectopic tissue.
588
590
Influence of Antiangiogenic Agent Bevacizumab on Endometriosis Experimentally Induced in Rats Rosa-e-Silva JC, Zani ACT, Valerio FP, Meola J, Poli-Neto OB, Candidodos-Reis FJ, Nogueira AA. Gynecology and Obstetrics, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil
Influence of Antiangiogenic Agent Propranolol on Endometriosis Experimentally Induced in Rats Rosa-e-Silva JC, Zanardi JVC, Fortunato GG, Meola J, Poli-Neto OB, Candido-dos-Reis FJ, Nogueira AA. Gynecology and Obstetrics, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil
Study Objective: The aim of this study is to evaluate the effect of antiangiogenic agent bevacizumab in two different dosages on endometriotic lesions induced in rats. Design: Experimental study. Setting: This study was carried out in the Department of Obstetrics and Gynecology and Experimental Surgery Sector, in the School of Medicine of Ribeir~ao Preto, University of S~ao Paulo. Patients: 30 female Wistar rats. Intervention: Endometriotic lesions was first induced in the 30 rats by the implantation of endometrium tissue on the peritoneum. The rats were, then, allocated in 3 groups of 10 rats. The first was the control group, in which no medication was used after surgery. The second received the drug twice a month and the third received the same drug (bevacizumab) twice a week. Measurements and Main Results: At the time of removal of the lesion, it was observed that the control group developed typical endometriotic lesions, while both medication groups did not present them. All the lesions and a piece of uterine tissue were removed of all the 30 rats for further genetic and pathologic evaluation of the angiogenic genes and receptors expression. Conclusion: The endometriotic lesions are richly vascularized and many studies have proven that the emergence of new vessels is central. This study showed that there is an important difference between the appearance of the lesions when using or not antiangiogenic medication. Further evaluation will prove theses differences on molecular levels.
Study Objective: To assess the anti-angiogenic effect of two doses of propranolol on the endometriosis lesions induced in female rats compared to controls. Design: Experimental study. Setting: Experimental Surgery Sector. Patients: 30 female Wistar rats. Intervention: We studied the effect of this drug on differentiation markers, invasion, cell proliferation and apoptosis by immunohistochemical staining, and also the influence on the adhesion, motility and angiogenesis of endometriotic lesions by extracting total RNA, cDNA synthesis and quantification by real time PCR. We used 30 Wistar adult rats, virgin females and submitted to laparotomy for induction of endometriosis lesions. The rats were listed in three groups, and sacrificed after 14 days of low dose treatment (PB,n=10) and high dose (PA,n=10) of propranolol; and control group (C,n=10) without treatment. The lesions were excised for histological analysis with the contralateral uterine horn and confirmed the presence of endometrial glandular and stromal tissue. Immunohistochemical reactions were performed for MMP-9, Metallothionein, TIMP-2, Caspase-8 and PCNA, and evaluation of gene expression by VEGF, CALD1 PCNA, TNF and SPARC primes, in tissue from injury and uterus. Measurements and Main Results: In this study, for the material obtained from lesions, was observed a reduction in immunohistochemical staining for MMP-9 in the control group (100% positive) to the treated groups - PA 60% of positivity and PB = 11.1% positive; p\0.05. Moreover, for metallothionein, with 60% of positivity in the control group, 10% and 11.1% for PA, PB, p\0.05; the quantification by real-time PCR, to the significance level of 0.05, there was a decrease in gene expression of PCNA (median control group=1.04; PA =0.32; PB=0.69). In other immunohistochemistry markers and quantification of gene expression to the material from injury no significant differences were observed. Conclusion: Treatment with anti-angiogenic drugs offers new prospects for therapeutic approach for patients with endometriosis.
589 Increased Expression of ID2, PRELP, and SMOC2 Genes in Patients with Endometriosis Rosa-e-Silva JC, Ara ujo FM, Meola J, Ferriani RA, Nogueira AA. Gynecology and Obstetrics, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil Study Objective: Endometriosis is a benign, estrogen-dependent disease with symptoms such as pelvic pain and infertility, and it is characterized by the ectopic distribution of endometrial tissue. This study aimed to analyze the gene expression ID2, PRELP and SMOC2 in women with and without endometriosis. Design: Cross-sectional study. Setting: A tertiary referral hospital. Patients: Women with or without endometriosis. Intervention: The expression of the ID2, PRELP, and SMOC2 genes was compared between the endometrium of women without endometriosis in
591 Spontaneous Fertility After Expectant or Surgical Management of Rectovaginal Endometriosis with/ without Ovarian Endometrioma: A 7-Year Retrospective Analysis Leone Roberti Maggiore U, Scala C, Tafi E, Racca A, Venturini PL, Ferrero S. Department of Neurosciences, Rehabilitation, Ophthalmology,