Induced hypocalcaemia and intact PTH responsiveness in patients with primary hyperparathyroidism, primary hypoparathyroidism and normal man

211

218

IFlDUCED HYPOCALCAEMIA AND INTACT PTH RESPONSlVENESS IN PATIENTS WITH PRIMARY MYPERPARATHYROIDISM, PRIMARY tIYPOPARATHYROIDISM AND NORMAL MAN. PSchwarr. H.A. btenscn. P.

OCTREOT I DE, AN INHIBITOR OF GROWTH HORMONE SECRET ION. ENHANCES POSlTlVE CALCIUM BALANCE IN CHILDREN. G. Palmieri. D. Nutting. 1. Bittle. M. IZdwardy, H. Sacks. E. Schriock. T. Rertorini. Departments of Medicine, Physiology, Pediatrics, and Neurology, College of Medicine, University of Tennessee, Memphis, USA. !.s part of an investigation on t$e effects of growth hormone (GH) inhibition by octreotide (0~) in children with Duchenne muscular dystrophy (DMD), we determined elemental balance in 7 DMD boys, age 8-17 y. After adaptation to a diet constant in protein, calories and Ca (24 mg/kg/d). the boys began two, 6 d balance periods: control (0. and Oc, marked by non-absorbable dyes. At the end of the C period, Oc was started, 2 yg/kg, subcutaneously, every 8 h, and 2 d later the Oc balance period began. Oc markedly blunted spontaneous and GHRH - induced GH secretion, and serum IGF- 1 (Cel.33 + 0.40 U/ml; Oc-0.64 + 0.19; Pc0.05). During OS, daily urinary Ca excretion fell (C-94 2 27 mg/d; Oc = 52 ;t. If; P-0.053). Fecal Ca did not increase I Cm354 2 45 mg/d (46% of dietary Ca); Oc = 305 + 37 (39% of dietary Cal; P = 0. Il. Thus the net effect of Oc was lo enhance Ca retel,tion (C- +3 16 2 56 mg/d; Oc = +4 1 1 2 36; P; 0.04 1. Oc did not affect urinary Na, K., or P excretion, or creatinine clearance. Since GH and growth factors, such as IGF- I, have been implicated in bone formation, the observed enhanced positive Ca balance during inhibition of GH secretion and low serum IGF- t is noteworthy. Oc, whatever the mechanism(s), could have potential in the treatment of hypercalciurias and osteopenias.

McNair. LTransbull,
WC found a significantdiffcrcnce in bosc-line mcasurcmentsof B-Caz+ and Se PTH(l-84) bctwccn PHPT, PHypoPT and controls (p-zO.01).During the TSCC the S-PTH(l-84) in PHPT, PHypoPT and conlrols peaked alter S-10 min to a level 4-6 times the inilial concentration and then declined lo ;I constant level 2-3 times the initial concentrationduring the steady-stateperiod. Our results indicatesthat the PHypoPT and PHPT is caused by a set-point error, and that the TSCC technique is suflicicnt in order to classify the me(abolic dysfunction.

219

220

1,25-DIRYDROXX VITAMIN D AND OTHER INDUCORS INDUCE DIFFERENTIATION OF ~~-60 CELLS INTO DIFFERENT MATURE CELLS.

OEIERNINAPI@l OF PlRIDINOLlNE (HP) AND

DEOYIPIRIDINOLINE (Lp) ,U URINE II

AUIQ(AtIC LlqUID XlLlD EXlRACT1ON PRDCEDURE 1N ,WSlWAlloW U,," "pLC. b

AMiMi,

II.Riaoldi. S, Frignsni. II.Arriqmi. C. Csstiqli@Bl Istituto Riccrche LPS, C.Brlrwm (Ml), Italy.

Y.Xue,*J.Tian and X.S.Li.Beijing Institute matology,lOOOj5 and Orthopedica.*Institute Medical Science, AMMS, P.R.China.

Trauof Basic

HP and LP lra interesting cycled mninaacidm which Diw

of

Human promyelocytic leukemia cells,%-69 can he induced differentiation hy l,25-(OH),D,(lO M), RA (1O”M) and TPA(lO”M) respectively into mature manulocyte-like,monocyte-like and macrophaFe-like cells in vitro. We studied the chanRee of the cell prowth,hIstnchemical stain and reduction of NRT. HL-60 cello exhihited distinctly morpholoRic aland electron microscopy after 1,25(O ters on lie!’ H),DI treatment such that most cellu Irecame irrefular and conclusively identified aa macrophages and the ratio of nucleus to cytoplasm decreased. 1,25(0tl), D1 or RA caused a simificant increase of reduction of NBT, ahout 13 to 1C fold neater than that in untreated cellg.d-NAE and ACP activities were respectively 11 and 4.R fold higher than that in untreated cells. Moreover we also studied the chanpes of cell cycle. The histop;ram of DNA and protein distribution anolysed hy flow cytometry demonstrated that protein synthesis decreased 61% and 20% and cells respectively inercrased 2.7 and 3 7 fold in co $ Cl phlse and respectively decreased 44% and 61% in synthetic phase on day 4 after 1,25( OH).D, and RA treatment as compared with untreated ccl la.

cros0-lin!+3In bonr

collsaen nvt"ra form erd nay br related to different metAb4lc bon dlAreese.

HP wvJ LP were separated, acrtonltrils

In YII~C~ (23:7?;

(HFBA) ~II ionqirinp Injected

for

Allquots 1WC

agent.

HP mnd 10 pwl

and 0.02M n-hrptrfluar&tyrle

IS slumt

for LP; detrt(m

Nrdrotysstn

acetlc

acid,

2.prop,wel.

After

artracrion

WI

Nf

uere evsporeted rcrtlc 1:l:l;

these NMI uith

acid v/v),

opcrrtlon

200

mg of

1.5

Purified

SW

,wv:~

the

C~IIUIOSU

crrtridger

then 3.15

ml of eech snplc

fiftcr

mirturc,

a scr(es

of

5 uashlngs with

ctutedby

2.4 ml of distill@d

dried

recowtituted

w,d

Mwtyxed by HPLC.

129

p?M&re

with

(IAtW

ml

of

flinttnlccted. with

n-bulmol

at

t&S ml 6N

u~tlurm

CPl

uew

ecnditlwd

t&n%

hhre.3 tfn+r.

celluloe~ 60 Wins

twice

of htirolfld

0.5 ml of

md

proWMe

fo abia to tfW

2.5 ml of brtwwllc for

acid of SJWI~

values of 37% MCI

and rccrnstituted

the wt~l~tlc

Ihis

tutenolfc

utantie

w3iRg

,O.S ml of but@nolIc mlxturb WvbrtQml.

Labxatories). 9 hro.

¶W

tn UI sqrvl

ASPEC system IGitson) ufn(l dlw6twblc

nsde by II

packed

limlt WI

hydrolyred

Cl8 eolum

to 30lmolallqwt

Peak WCBS were Llncar

NCI, sddrd of 0.5 ml glac~ol

rlrtrldgw,

on 83 reverse-phase

v/v)

of urine (0.5 ml) we

overnight.

glacial

tn 15 minutes,

4th

urlns

Ihe SPE

emtr&tth (5Jolarlr 08&n, 2.5

in

111 of

WI5 L&&d.

mixture,HPmdLPwe lh@ @lLUte MUI COlt’ZCtd,

200 &I of 1% HfBA uBt@r RDtutian @xl 30 iat WIG