Inflammatory and oxidative stress markers among children and adolescents with bipolar disorder

Molecular and cellular P.1.021 Inflammatory and oxidative stress markers among children and adolescents with bipolar disorder F. Hernandez Alvarez1*, G. Salazar de Pablo1, R. Vicente Moreno1, A. Del Rey Mejías1, K. McDowell2, J. Vidal De la Fuente1, A. Espliego Felipe1, L. Torres Jímenez1, C. Moreno Ruiz1. 1Hospital General Universitario Gregorio Marañón- IiSGM- CIBERSAM- Facultad de Medicina- Universidad Complutense- Madrid- Spain, Child and Adolescent Psychiatry Department, Madrid, Spain; 2Universidad Complutense- Madrid- Spain, Pharmacology Department, Madrid, Spain Introduction: Despite burgeoning literature in adults, little is known regarding inflammatory and oxidative stress markers among children and adolescents with bipolar disorder (BD). In recent years it has been found that there is an activation of several components of the immune response and inflammation markers in adolescents with BD [1]. Besides, earlier studies suggest that oxidative stress plays a role in its pathophysiology [2]. Thereafter, inflammatory and oxidative stress markers may be relevant to bipolar disorder characteristics as well as other clinical characteristics among children and adolescents with BD [3]. Objectives: The primary objective was to examine inflammatory and oxidative stress markers in a sample of children and adolescents with BD and in a group of healthy controls. The secondary objective was to analyse the correlation between inflammatory/oxidative stress markers and the presence of psychotic symptoms. Method: Subjects were 32 children and adolescents with bipolar disorder (14.4 ± 3.8 years) and 20 healthy controls (14.8 ± 2.0) matched by sex and age. DSM-IV diagnoses were determined using the Schedule for Affective Disorders and Schizophrenia for School-Age Children (K-SADS). The clinical evaluation included Positive and Negative Syndrome Scale (PANSS), Young Mania Rating Scale (YMRS), Hamilton Scale Rating for Depression (HDRS), Clinical Global Impression Severity Scale (CGI-S) and c-GAS (Children’s Global Assessment Scale). Inflammatory and oxidative stress markers were assayed. Categorical variables were compared using χ2 test. Quantitative variables were compared with nonparametric Mann-Whitney U test or Student’s t-test. A logistic regression was used to analyse the relation between significant markers and psychotic symptoms. Data was analysed with the IBM SPSS software, version 21. Results: Significant differences were found between patients with bipolar disorder and healthy controls in serum levels of NO2 ( p < 0.0001), MDA ( p = 0.003), TNF-α ( p < 0.001), IL-1β ( p < 0.001), IL-6 ( p = 0.015),

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TACE ( p = 0.001), 15dPGJ2 ( p = 0.003), TAS ( p < 0.001), TNFαRII ( p = 0.029), GPX ( p = 0.026), GSH ( p = 0.026), GSGG ( p < 0.001). In a second analysis, significant differences were found between patients with bipolar disorder according to the presence of psychotic symptoms in the following markers: TAS ( p = 0.001), GSH ( p = 0.002) and GSGG ( p = 0.037). A forward stepwise logistic regression was performed to analyse the relation between inflammatory and oxidative stress markers and psychotic symptoms. In a first step, we included those markers significantly different between psychotic and non-psychotic patients (TAS, GSH and GSGG). The only variable which improved the null model was GSH (B = −0.05; p = 0.04; Classification correct = 80%). However, when we controlled by clinical scale scores (PANSS, YMRS, HDRS, CGI-S, C-GAS), the significance disappeared, more specifically the association with GSH (B = −0.03; sig = 0.26) did not reach statistically significance after controlling by PANSS-Negative (B = −0.75; p = 0.04). Conclusion: Our findings support the role of oxidative stress and inflammation in early-onset BD regardless of the presence of psychotic symptoms. Validating these potential biomarkers as clinically relevant will require repeated measures and incorporation of relevant covariates. Reference(s) [1] Miklowitz, D.J., Portnoff, L.C., Armstrong, C.C., 2016. Inflammatory cytokines and nuclear factorkappa B activation in adolescents with bipolar and major depressive disorders. Psychiatric Res 241, 315–22. [2] Brown, N., Andreazza, A., Young, L., 2014. An updated meta-analysis of oxidative stress markers in bipolar disorder. Psychiatry Research, 218(1–2), 61–68. [3] Goldstein, B.I., Lotrich, F., Axelson, D.A., 2015. Inflammatory markers among adolescents and young adults with bipolar spectrum disorders. J Clin Psychiatry 76(11), 1556–63. P.1.022 Tau protein as a key element in stress-induced dendritic atrophy and suppression of adult hippocampal neurogenesis C. Dioli1*, J.M. Bessa1, L. Pinto1, N. Sousa1, I. Sotiropoulos1. 1Life and Health Sciences Research Institute ICVS, Neuroscience Research Domain, Braga, Portugal Neuronal and synaptic malfunction is a key pathomechanism in both depressive and Alzheimer’s disease (AD)