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Inflammatory potential of diet and risk of laryngeal cancer in a case–control study from Italy
EACR24 Poster Sessions / European Journal of Cancer 61, Suppl. 1 (2016) S9–S218
Sunday 10 July 2016 Poster Session
Prevention and Early Detection I 685 A novel detection method of metastatic cells in the cerebrospinal fluid of pediatric population with medulloblastoma using fluorescence lifetime imaging microscopy S. Gershanov1 , H. Toledano2 , S. Michowiz3 , G. Yahav4 , O. Barinfeld5 , A. Hirshberg6 , M. Salmon-Divon1 , D. Fixler4 , N. Goldenberg-Cohen5 . 1 Ariel University, Molecular Biology, Ariel, Israel, 2 Schneider Children’s Medical Center of Israel, Pediatric Oncology, Petach Tikva, Israel, 3 Schneider Children’s Medical Center of Israel, Pediatric Neurosurgery, Petach Tikva, Israel, 4 Bar Ilan University, Faculty of Engineering and Institute of Nanotechnology and Advanced Materials, Ramat Gan, Israel, 5 Tel Aviv University, The Krieger Eye Research Laboratory- Felsenstein Medical Research Center- Sakcler school of medicine, Tel Aviv, Israel, 6 Tel Aviv University, Oral Pathology and Oral Medicine- Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv, Israel Medulloblastoma (MB) is the most common malignant brain tumor in children, and one of the deadliest. About one third of children with MB already demonstrate metastatic spread at diagnosis and approximately 40% will experience metastatic tumor recurrence post treatment. Another highly malignant tumor of childhood is the atypical teratoid/rhabdoid tumor (ATRT) usually diagnosed in the first 2 years of life. Despite recent advances in treatment, patients with tumor dissemination have a poor survival rate. Moreover, early metastatic spread is often undetectable by imaging or cytology. The purpose of this study is to demonstrate the capabilities of a novel diagnostic method to improve early detection and monitoring of metastatic spread, using fluorescence lifetime imaging microscopy (FILM). Cells extracted from tumor at diagnosis and cerebrospinal fluid (CSF), 2 weeks after surgery and repeatedly during and after chemo/radiotherapy, of 15 children diagnosed with MB and 2 with ATRT. The cells obtained were subjected to nuclear staining by DAPI followed by fluorescence lifetime (FLT) measurement. FLT of DAPI was divided into 3 subgroups: prolonged, medium and short. Prolonged FLT was found predominantly in cells from the original tumor cells (median 4.27±0.28 ns) and in the CSF cells from metastatic children obtained before chemo/radiotherapy (median 6.28±0.22 ns); medium FLT in immune system cells from non-oncology pediatric patients (median 2.6±0.04 ns) who served as controls, also in CSF cells from non-metastatic children before chemo/radiotherapy (median 2.62±0.23 ns) and following treatment (median 2.62±0.21 ns). Short FLT predominated in CSF samples from metastatic children post treatment (median 2.4±0.29 ns), either chemotherapy or craniospinal radiation. FLIM is simple and fast assay with the potential to identify tumor spread in the CSF of children with malignant brain tumors. FLT measurements change according to treatment, hence may guide personalized therapy, improve outcome and increase survival. No conflict of interest. 686 Awareness and understanding of disease among hospitalized cancer patients in Pakistan
S153
illness. Although the patients were more satisfied with care than the information they had received, awareness was not related to satisfaction (p > 0.05). Most of the patients (71.0%) were not satisfied with the quantity and quality of the information they had received from their health care provider. Conclusions: Our findings suggest that although cancer patients want and need to have adequate information regarding their disease, the amount and quality of information they receive is not optimal leading to adoption of passive information seeking strategy causing misconceptions about disease. No conflict of interest. 687 Inflammatory potential of diet and risk of laryngeal cancer in a case–control study from Italy N. Shivappa1 , J. Hebert1 , V. Rosato2 , D. Serraino3 , C. La Vecchia2 . 1 University of South Carolina, Epidemiology and Biostatistics, Columbia, USA, 2 Universita‘ degli Studi di Milano, Department of Clinical Sciences and Community Health, Milan, Italy, 3 Centro di Riferimento Oncologico, Unit of Epidemiology and Biostatistics, Aviano, Italy Background: Besides tobacco and alcohol, diet and inflammation have been suggested to be important risk factors for laryngeal cancer. Materials and Methods: In this study, we examined the role of a dietary inflammatory index (DII) to predict laryngeal cancer in a multicentre case– control study conducted between 1992 and 2000 in Italy. This study included 460 cases with incident, histologically confirmed laryngeal cancer, and 1088 controls hospitalized for acute non-neoplastic diseases unrelated to tobacco and alcohol consumption. The DII was computed based on dietary intake assessed by a validated 78-item food frequency questionnaire. Logistic regression models were used to estimate odds ratios (ORs), and the corresponding 95% confidence intervals (CIs), adjusted for age, sex, study center, education, body mass index, tobacco smoking, alcohol drinking, and non-alcohol energy intake. Results: Subjects with higher DII scores (i.e., with a more pro-inflammatory diet) had a higher risk of laryngeal cancer. The OR was 3.30 (95% CI 2.06, 5.28; p for trend <0.0001) for the highest versus the lowest DII quartile. When DII was considered as a continuous variable, the OR was 1.22 (95% CI 1.14, 1.31) for a one unit (9%) increase. Stratified analyses produced slightly stronger associations between DII and laryngeal cancer risk among current smokers (ORQuartile4vs1 = 4.16), moderate drinkers (<4 alcoholic drinks/day) (ORQuartile4vs1 = 4.86), overweight subjects (ORQuartile4vs1 = 3.62), and among those with higher education (ORQuartile4vs1 = 3.92), in the absence of significant heterogeneity. We also observed a stronger combined effect of higher DII and smoking or alcohol consumption on laryngeal cancer. Conclusions: These results indicate that a pro-inflammatory diet is associated with increased risk of laryngeal cancer. ´ Conflict of interest: Other Substantive Relationships: Dr. James R. Hebert owns controlling interest in Connecting Health Innovations LLC (CHI), a company planning to license the right to his invention of the dietary inflammatory index (DII) from the University of South Carolina in order to develop computer and smart phone applications for patient counseling and dietary intervention in clinical settings. Dr. Nitin Shivappa is an employee of CHI. 689 Blood-circulating nucleoprotein complexes: DNA and protein content in healthy donors and breast cancer patients
N.A. Jadoon1 , F.U. Sulehri2 , N.A. Shair3 , M. Hussain1 . 1 Ittefaq Hospital, Department of Medicine, Lahore, Pakistan, 2 Shalamr Hospital, Medicine, Lahore, Pakistan, 3 Services Hospital, Surgery, Lahore, Pakistan
O. Tutanov1 , S. Tamkovich1 , T. Duzhak2 , Y. Tsentalovich2 , P. Laktionov1 . 1 Institute of Chemical Biology and Fundamental Medicine Siberian Branch of the Ru, Laboratory of Molecular Medicine, Novosibirsk, Russian Federation, 2 International Tomography Center SB RAS, Group of proteomics and metabolomics, Novosibirsk, Russian Federation
Background: Information needs and understanding of hospitalized cancer patients have remained unexplored. The objective of this study was to assess the awareness of cancer patients regarding their disease and to evaluate their understanding of disease and information seeking behavior. Methods: We enrolled 232 adult cancer patients for the study to collect data using semi structured interview regarding their awareness and understanding of illness. Results: A majority of patients (87.8%) reported awareness of their diagnosis. Female patients, patients from urban areas, educated patients and those with longer duration of illness had significantly better knowledge of their disease as compared to the rest of the study group (p < 0.05). Presence of metastatic disease did not significantly alter the patients’ understanding of disease or their information seeking behavior. Age was found to significantly influence the understanding of current disease status and request for more information regarding disease. Most of the patients (82.2%) wanted their family to know about their diagnosis while a few (4.8%) wished their friends to have knowledge about their illness. There was limited use of active information seeking strategy by patients (from medical books, internet, help lines etc) with more reliance on passive information seeking strategies (friends, other patients, newspaper, TV etc) which may have lead to the misconceptions patients had about their
Cell-free DNA (cfDNA) was found to circulate in blood being packed in apoptotic bodies or nucleosomes. Except from histones only few serum proteins (Bulter P, 1990) were shown to be potentially involved in cfDNA binding and circulation although a lot of cellular and plasma proteins could bind DNA or histones. In the current study we have investigated DNA and protein content of deoxyribonucleoprotein complexes (DNPC) circulating in blood of healthy donors (HD) and breast cancer patients (BCP). DNPC were isolated from blood plasma of HD and primary BCP by affinity chromatography using immobilized polyclonal anti-histone antibodies. DNA isolated from NPC was analyzed by Agilent 2100 Bioanalyser TM using High Sensitivity DNA Kit, proteins were identified using MALDI-TOF mass-spectrometry after 10−20% SDS-PAGE. 170–180 b.p. DNA was found to be main component of DNPC circulating in plasma of HD, whereas equal amount of 170–180 b.p. and more than 6 k.b.p. DNA was found in DNPC from blood of BCP, more than 200 additional proteins (excluding histones) were identified with a reliable score in DNPC of HD and BCP, morever considerable amount of identified proteins are DNA-binding or containing DNA-binding motifs. Seven proteins (G1/S-pecific cyclin-E2, Rho GTPaseactivating protein 30, NADP-dependent malic enzyme, SHC SH2
Sunday 10 July 2016 Poster Session
Prevention and Early Detection I 685 A novel detection method of metastatic cells in the cerebrospinal fluid of pediatric population with medulloblastoma using fluorescence lifetime imaging microscopy S. Gershanov1 , H. Toledano2 , S. Michowiz3 , G. Yahav4 , O. Barinfeld5 , A. Hirshberg6 , M. Salmon-Divon1 , D. Fixler4 , N. Goldenberg-Cohen5 . 1 Ariel University, Molecular Biology, Ariel, Israel, 2 Schneider Children’s Medical Center of Israel, Pediatric Oncology, Petach Tikva, Israel, 3 Schneider Children’s Medical Center of Israel, Pediatric Neurosurgery, Petach Tikva, Israel, 4 Bar Ilan University, Faculty of Engineering and Institute of Nanotechnology and Advanced Materials, Ramat Gan, Israel, 5 Tel Aviv University, The Krieger Eye Research Laboratory- Felsenstein Medical Research Center- Sakcler school of medicine, Tel Aviv, Israel, 6 Tel Aviv University, Oral Pathology and Oral Medicine- Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv, Israel Medulloblastoma (MB) is the most common malignant brain tumor in children, and one of the deadliest. About one third of children with MB already demonstrate metastatic spread at diagnosis and approximately 40% will experience metastatic tumor recurrence post treatment. Another highly malignant tumor of childhood is the atypical teratoid/rhabdoid tumor (ATRT) usually diagnosed in the first 2 years of life. Despite recent advances in treatment, patients with tumor dissemination have a poor survival rate. Moreover, early metastatic spread is often undetectable by imaging or cytology. The purpose of this study is to demonstrate the capabilities of a novel diagnostic method to improve early detection and monitoring of metastatic spread, using fluorescence lifetime imaging microscopy (FILM). Cells extracted from tumor at diagnosis and cerebrospinal fluid (CSF), 2 weeks after surgery and repeatedly during and after chemo/radiotherapy, of 15 children diagnosed with MB and 2 with ATRT. The cells obtained were subjected to nuclear staining by DAPI followed by fluorescence lifetime (FLT) measurement. FLT of DAPI was divided into 3 subgroups: prolonged, medium and short. Prolonged FLT was found predominantly in cells from the original tumor cells (median 4.27±0.28 ns) and in the CSF cells from metastatic children obtained before chemo/radiotherapy (median 6.28±0.22 ns); medium FLT in immune system cells from non-oncology pediatric patients (median 2.6±0.04 ns) who served as controls, also in CSF cells from non-metastatic children before chemo/radiotherapy (median 2.62±0.23 ns) and following treatment (median 2.62±0.21 ns). Short FLT predominated in CSF samples from metastatic children post treatment (median 2.4±0.29 ns), either chemotherapy or craniospinal radiation. FLIM is simple and fast assay with the potential to identify tumor spread in the CSF of children with malignant brain tumors. FLT measurements change according to treatment, hence may guide personalized therapy, improve outcome and increase survival. No conflict of interest. 686 Awareness and understanding of disease among hospitalized cancer patients in Pakistan
S153
illness. Although the patients were more satisfied with care than the information they had received, awareness was not related to satisfaction (p > 0.05). Most of the patients (71.0%) were not satisfied with the quantity and quality of the information they had received from their health care provider. Conclusions: Our findings suggest that although cancer patients want and need to have adequate information regarding their disease, the amount and quality of information they receive is not optimal leading to adoption of passive information seeking strategy causing misconceptions about disease. No conflict of interest. 687 Inflammatory potential of diet and risk of laryngeal cancer in a case–control study from Italy N. Shivappa1 , J. Hebert1 , V. Rosato2 , D. Serraino3 , C. La Vecchia2 . 1 University of South Carolina, Epidemiology and Biostatistics, Columbia, USA, 2 Universita‘ degli Studi di Milano, Department of Clinical Sciences and Community Health, Milan, Italy, 3 Centro di Riferimento Oncologico, Unit of Epidemiology and Biostatistics, Aviano, Italy Background: Besides tobacco and alcohol, diet and inflammation have been suggested to be important risk factors for laryngeal cancer. Materials and Methods: In this study, we examined the role of a dietary inflammatory index (DII) to predict laryngeal cancer in a multicentre case– control study conducted between 1992 and 2000 in Italy. This study included 460 cases with incident, histologically confirmed laryngeal cancer, and 1088 controls hospitalized for acute non-neoplastic diseases unrelated to tobacco and alcohol consumption. The DII was computed based on dietary intake assessed by a validated 78-item food frequency questionnaire. Logistic regression models were used to estimate odds ratios (ORs), and the corresponding 95% confidence intervals (CIs), adjusted for age, sex, study center, education, body mass index, tobacco smoking, alcohol drinking, and non-alcohol energy intake. Results: Subjects with higher DII scores (i.e., with a more pro-inflammatory diet) had a higher risk of laryngeal cancer. The OR was 3.30 (95% CI 2.06, 5.28; p for trend <0.0001) for the highest versus the lowest DII quartile. When DII was considered as a continuous variable, the OR was 1.22 (95% CI 1.14, 1.31) for a one unit (9%) increase. Stratified analyses produced slightly stronger associations between DII and laryngeal cancer risk among current smokers (ORQuartile4vs1 = 4.16), moderate drinkers (<4 alcoholic drinks/day) (ORQuartile4vs1 = 4.86), overweight subjects (ORQuartile4vs1 = 3.62), and among those with higher education (ORQuartile4vs1 = 3.92), in the absence of significant heterogeneity. We also observed a stronger combined effect of higher DII and smoking or alcohol consumption on laryngeal cancer. Conclusions: These results indicate that a pro-inflammatory diet is associated with increased risk of laryngeal cancer. ´ Conflict of interest: Other Substantive Relationships: Dr. James R. Hebert owns controlling interest in Connecting Health Innovations LLC (CHI), a company planning to license the right to his invention of the dietary inflammatory index (DII) from the University of South Carolina in order to develop computer and smart phone applications for patient counseling and dietary intervention in clinical settings. Dr. Nitin Shivappa is an employee of CHI. 689 Blood-circulating nucleoprotein complexes: DNA and protein content in healthy donors and breast cancer patients
N.A. Jadoon1 , F.U. Sulehri2 , N.A. Shair3 , M. Hussain1 . 1 Ittefaq Hospital, Department of Medicine, Lahore, Pakistan, 2 Shalamr Hospital, Medicine, Lahore, Pakistan, 3 Services Hospital, Surgery, Lahore, Pakistan
O. Tutanov1 , S. Tamkovich1 , T. Duzhak2 , Y. Tsentalovich2 , P. Laktionov1 . 1 Institute of Chemical Biology and Fundamental Medicine Siberian Branch of the Ru, Laboratory of Molecular Medicine, Novosibirsk, Russian Federation, 2 International Tomography Center SB RAS, Group of proteomics and metabolomics, Novosibirsk, Russian Federation
Background: Information needs and understanding of hospitalized cancer patients have remained unexplored. The objective of this study was to assess the awareness of cancer patients regarding their disease and to evaluate their understanding of disease and information seeking behavior. Methods: We enrolled 232 adult cancer patients for the study to collect data using semi structured interview regarding their awareness and understanding of illness. Results: A majority of patients (87.8%) reported awareness of their diagnosis. Female patients, patients from urban areas, educated patients and those with longer duration of illness had significantly better knowledge of their disease as compared to the rest of the study group (p < 0.05). Presence of metastatic disease did not significantly alter the patients’ understanding of disease or their information seeking behavior. Age was found to significantly influence the understanding of current disease status and request for more information regarding disease. Most of the patients (82.2%) wanted their family to know about their diagnosis while a few (4.8%) wished their friends to have knowledge about their illness. There was limited use of active information seeking strategy by patients (from medical books, internet, help lines etc) with more reliance on passive information seeking strategies (friends, other patients, newspaper, TV etc) which may have lead to the misconceptions patients had about their
Cell-free DNA (cfDNA) was found to circulate in blood being packed in apoptotic bodies or nucleosomes. Except from histones only few serum proteins (Bulter P, 1990) were shown to be potentially involved in cfDNA binding and circulation although a lot of cellular and plasma proteins could bind DNA or histones. In the current study we have investigated DNA and protein content of deoxyribonucleoprotein complexes (DNPC) circulating in blood of healthy donors (HD) and breast cancer patients (BCP). DNPC were isolated from blood plasma of HD and primary BCP by affinity chromatography using immobilized polyclonal anti-histone antibodies. DNA isolated from NPC was analyzed by Agilent 2100 Bioanalyser TM using High Sensitivity DNA Kit, proteins were identified using MALDI-TOF mass-spectrometry after 10−20% SDS-PAGE. 170–180 b.p. DNA was found to be main component of DNPC circulating in plasma of HD, whereas equal amount of 170–180 b.p. and more than 6 k.b.p. DNA was found in DNPC from blood of BCP, more than 200 additional proteins (excluding histones) were identified with a reliable score in DNPC of HD and BCP, morever considerable amount of identified proteins are DNA-binding or containing DNA-binding motifs. Seven proteins (G1/S-pecific cyclin-E2, Rho GTPaseactivating protein 30, NADP-dependent malic enzyme, SHC SH2