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Influence of diabetes mellitus on endodontic materials tissue response
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d e n t a l m a t e r i a l s 2 9 S ( 2 0 1 3 ) e1–e96
hardness decrease directly proportional to thickness. The second hypothesis (2) is rejected since SDR showed a significantly lower contraction stress than other materials. Material
Sonicfil Tetric bulk SDR X-tra base Filtek bulk
Top surface microhardness
Bottom surface microhardness
Contraction stress (MPa)
90.9 106.4 54.9 94.5 97.7
45.4 45.2 28.9 56.5 36.9
0.93 0.82 0.61 0.89 0.88
http://dx.doi.org/10.1016/j.dental.2013.08.086 86 Influence of diabetes mellitus on endodontic materials tissue response J.E. Gomes-Filho ∗ , I.O.A. Queiroz, S. Watanabe, L.T.A. Cintra, E. Dezan-Junior, C.S. Lodi, E. Ervolino University Estadual Paulista, Brazil Purpose: The aim of this study was to evaluate the influence of diabetes mellitus on tissue response and mineralization ability of Sealapex® (Sybron Endo, Glendora, USA), MTA Angelus® (Angelus, Londrina, Brazil) and MTA Fillapex® (Angelus, Londrina, Brazil) sealers. Methods and materials: Twenty-four Wistar rats were divided into two groups: diabetic induced by Alloxan and nondiabetic. The materials were placed in polyethylene tubes and implanted into dorsal connective tissue of rats for 7 and 30 days. The animals killed after 30 days received injection with calcein on day 7, alizarin on day 14 and oxytretacycline hydrochloride on day 21. After animal killing, the specimens were prepared for histologic analysis with hematoxylin and eosin, Von Kossa technique, and not stained for polarized light or fluorescence. Results: At the 7th day, it was observed similar inflammatory reactions among all groups characterized by a moderate inflammatory response and on 30 days, it was found a mild inflammatory response. Diabetic and non-diabetic groups exhibited positive structures on Von Kossa and Polarized light analysis either on 7 and 30 days. It was found difference on fluorescence intensity between each materials and control independently of the fluorochrome. Sealapex® and MTA Fillapex® showed more fluorescence intensity than MTA Angelus® (p < 0.05) for all fluorochromes in non-diabetic group and just for alizarin and oxytretacycline in diabetic group. It was not observed difference between Sealapex® and MTA Fillapex® groups for all fluorochrome independent of the diabetic condition. Conclusion: It was concluded that diabetes mellitus did not influence the tissue response or mineralization stimulated by Sealapex® or MTA Angelus® or MTA Fillapex® . http://dx.doi.org/10.1016/j.dental.2013.08.087
87 Influence of application of Nd:YAG laser with adhesive system on intrapulpal temperature S.E.P. Goncalves ∗ , T.M. Silva, L.L. Goncalves, B.M. Fonseca, S.R.M. Esteves, M.A.R.L. Esper, D.C. Barcellos, M.F.R.L. Huhtala, A.P.M. Gomes, E. Bresciani UNESP - University Estadual Paulista, Brazil Purpose: The aim of the present study was to evaluate the effects of Nd:YAG laser irradiation and simulated pulpal pressure on intrapulpal temperature Methods and materials: One hundred healthy human molars were sectioned 1 mm below the cementoenamel junction to separate the crown of the root portion and sectioned parallel to the occlusal surface to expose 2 mm thickness of dentin. The 100 samples were divided into two groups (n = 50) according to presence and absence of simulated pulpal pressure. Each group was divided into five groups (n = 10) depending on the variation in energy parameters of Nd:YAG laser (60, 80, 100, 120, 140 mJ). The samples were treated with: phosphoric acid 37% (Scotchbond Universal), adhesive system (Scotchbond Universal/3M ESPE), irradiation with Nd:YAG laser for 60 s and light cure for 10 s. Intrapulpal temperature was evaluated through a digital thermometer with thermocouple type K (MT-507, Minipa) positioned in the highest pulp horn. To simulate pulpal pressure, a device filled with deionized water was positioned 15 cm from the level of pulp chamber. For the group without simulated pulpal pressure, pulp chamber was filled with thermal paste. Statistical analysis consisted of two-way analysis of variance (ANOVA; laser energy parameters and pulpal pressure) and Tukey’s test with a significance level of 5% (p < 0.05). Results: ANOVA revealed statistically significant effect in the temperature range (p < 0.05). The highest average (14.30 ◦ C) was observed in the group irradiated with 140 mJ without pulpal pressure simulation, while the lowest average (3.90 ◦ C) was observed in the group irradiated at 60 mJ with simulated pulpal pressure. It was observed that the groups treated with simulated pulpal pressure had lower mean values, and the mean values increased with increasing laser energy parameters. Conclusion: Thus, this study concluded that the variation in temperature during laser irradiation is dependent on the energy parameters of Nd:YAG laser and intrapulpal pressure simulation. http://dx.doi.org/10.1016/j.dental.2013.08.088
d e n t a l m a t e r i a l s 2 9 S ( 2 0 1 3 ) e1–e96
hardness decrease directly proportional to thickness. The second hypothesis (2) is rejected since SDR showed a significantly lower contraction stress than other materials. Material
Sonicfil Tetric bulk SDR X-tra base Filtek bulk
Top surface microhardness
Bottom surface microhardness
Contraction stress (MPa)
90.9 106.4 54.9 94.5 97.7
45.4 45.2 28.9 56.5 36.9
0.93 0.82 0.61 0.89 0.88
http://dx.doi.org/10.1016/j.dental.2013.08.086 86 Influence of diabetes mellitus on endodontic materials tissue response J.E. Gomes-Filho ∗ , I.O.A. Queiroz, S. Watanabe, L.T.A. Cintra, E. Dezan-Junior, C.S. Lodi, E. Ervolino University Estadual Paulista, Brazil Purpose: The aim of this study was to evaluate the influence of diabetes mellitus on tissue response and mineralization ability of Sealapex® (Sybron Endo, Glendora, USA), MTA Angelus® (Angelus, Londrina, Brazil) and MTA Fillapex® (Angelus, Londrina, Brazil) sealers. Methods and materials: Twenty-four Wistar rats were divided into two groups: diabetic induced by Alloxan and nondiabetic. The materials were placed in polyethylene tubes and implanted into dorsal connective tissue of rats for 7 and 30 days. The animals killed after 30 days received injection with calcein on day 7, alizarin on day 14 and oxytretacycline hydrochloride on day 21. After animal killing, the specimens were prepared for histologic analysis with hematoxylin and eosin, Von Kossa technique, and not stained for polarized light or fluorescence. Results: At the 7th day, it was observed similar inflammatory reactions among all groups characterized by a moderate inflammatory response and on 30 days, it was found a mild inflammatory response. Diabetic and non-diabetic groups exhibited positive structures on Von Kossa and Polarized light analysis either on 7 and 30 days. It was found difference on fluorescence intensity between each materials and control independently of the fluorochrome. Sealapex® and MTA Fillapex® showed more fluorescence intensity than MTA Angelus® (p < 0.05) for all fluorochromes in non-diabetic group and just for alizarin and oxytretacycline in diabetic group. It was not observed difference between Sealapex® and MTA Fillapex® groups for all fluorochrome independent of the diabetic condition. Conclusion: It was concluded that diabetes mellitus did not influence the tissue response or mineralization stimulated by Sealapex® or MTA Angelus® or MTA Fillapex® . http://dx.doi.org/10.1016/j.dental.2013.08.087
87 Influence of application of Nd:YAG laser with adhesive system on intrapulpal temperature S.E.P. Goncalves ∗ , T.M. Silva, L.L. Goncalves, B.M. Fonseca, S.R.M. Esteves, M.A.R.L. Esper, D.C. Barcellos, M.F.R.L. Huhtala, A.P.M. Gomes, E. Bresciani UNESP - University Estadual Paulista, Brazil Purpose: The aim of the present study was to evaluate the effects of Nd:YAG laser irradiation and simulated pulpal pressure on intrapulpal temperature Methods and materials: One hundred healthy human molars were sectioned 1 mm below the cementoenamel junction to separate the crown of the root portion and sectioned parallel to the occlusal surface to expose 2 mm thickness of dentin. The 100 samples were divided into two groups (n = 50) according to presence and absence of simulated pulpal pressure. Each group was divided into five groups (n = 10) depending on the variation in energy parameters of Nd:YAG laser (60, 80, 100, 120, 140 mJ). The samples were treated with: phosphoric acid 37% (Scotchbond Universal), adhesive system (Scotchbond Universal/3M ESPE), irradiation with Nd:YAG laser for 60 s and light cure for 10 s. Intrapulpal temperature was evaluated through a digital thermometer with thermocouple type K (MT-507, Minipa) positioned in the highest pulp horn. To simulate pulpal pressure, a device filled with deionized water was positioned 15 cm from the level of pulp chamber. For the group without simulated pulpal pressure, pulp chamber was filled with thermal paste. Statistical analysis consisted of two-way analysis of variance (ANOVA; laser energy parameters and pulpal pressure) and Tukey’s test with a significance level of 5% (p < 0.05). Results: ANOVA revealed statistically significant effect in the temperature range (p < 0.05). The highest average (14.30 ◦ C) was observed in the group irradiated with 140 mJ without pulpal pressure simulation, while the lowest average (3.90 ◦ C) was observed in the group irradiated at 60 mJ with simulated pulpal pressure. It was observed that the groups treated with simulated pulpal pressure had lower mean values, and the mean values increased with increasing laser energy parameters. Conclusion: Thus, this study concluded that the variation in temperature during laser irradiation is dependent on the energy parameters of Nd:YAG laser and intrapulpal pressure simulation. http://dx.doi.org/10.1016/j.dental.2013.08.088