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APOB ratio
Poster session abstracts/Atherosclerosis 115 (SuppL ) (1995) $45-$129 P1 Nutrition
$47
170 PROTECTIVE EFFECT OF VITAMIN E ON AORTIC LIPID PEROXIDAT1ON AND ATHEROMA FORMATION IN HYPERCHOLESTEROLEMIC RABBITS L. Kazdov& D. Urbanovzl, A. Vr~ina, M. Mat~j~kov~i, V. Nowikov~i Institute for Clinical and Experimental Medicine, Prague, Czech Republic Free radical-mediated reactions, including lipid peroxidation, are considered to be important factors in the development of atherosclerosis. Therefore, the effect of antioxidant therapy with vitamin E on aortic tissue lipid peroxidation and atherosclerotic lesions development was investigated. Male New Zealand White rabbits were fed a chow diet (C) or atherogenic diet tAD, 0.5% of cholesterol) without or with vitamin E supplementation t A D + E , 60 mg/kg b.wt.) for 8 weeks. To produce more advanced lesions, in~nune-mediated endothelial injury was induced by i.v. injection of 250 mg/kg b.wt. of bovine serum albumin at the beginning of the experiment. We found that vitamin E supplementation had no effect on serum triglycerides tAD:2.3 _+ 0.5 vs AD+E:2.0 _+ 0.5 mmol/I n.s.) or hypercholesterolemia (AD:13.5 ::t 1.9 vs AD+E: 10.5 5 : 2 . 0 nunol/l n.s.). On the other hand, vitamin E decreased aortic tissue malondialdehyde levels tAD:0.29 _+ 0.03 vs AD+E:0.91 _+ 0.08 nunol/mg protein) and reduced aortic cholesterol content by 36% and lesion coverage by 29%. Histology of the lesions revealed lower intimal thickness and decreased foam cell and connective tissue accumulation. In conclusion, results indicate that a) vitamin E inhibited the development of atherosclerosis without lowering hyperlipidemia, b) this effect may be mediated through its antioxidant activity in the artery wall.
CHANGES IN HDL. APOAI. AND HDL SUBFRACTIONS BY REDUCTION OR ADDITION OF DIFFERENT DIETETIC FATTY ACIDS J. Millan', I Cortes, A. Mangas, P. Conthe'. A.Tejedor'. P. GarciaBarreno" Department of Internal Medicine: Hospital General Universitario "Gregorie Marafion" and University of Cadiz In a model of dietetic intervention over 40 women of 5 religious comnmnities, we have evaluated the influence of changes in dietetic afatty acids reduction of oleic acid with adition of linoleic acid, or reductitm ol [inoleic acid with adition of oleic acid without variation in saturated tart) acid fig"; ). carbohydrates (42%) or protein (15%. Caloric intake was not changed aud any change in corporal weight was observed HDL and HDL subfractions (HDL-2 and HDL-3) and correlation with ApoAI were evaluated in two times: period A (light decrease in oleic acid retake -4ml- by reduction of olive oil, with increase -6ml of linoleic acid by aditiun of sunflower oil) and period B (notorious reduction ol linoleic acid -22ml- with increase -20ml- of oleic acid intakel. Ratios {*l variation in biochemical parameters were more well correlated with the ml of fatty acid aditiones. MI of linoleic acid increased were correlated with % of variation of HDL {r -0,681 HDI.-2 i r - 0.74). HDL-3 (r~0.71) and ApoAI (r=-0.76) MI of o]eic acid increased were correlated with % of variation in HDL !r 0,75) HDL-2 {r~-0,51), HDL-3 i r - 0 . 7 4 ) and ApoAI ( r - 0 , 7 6 ) Correlalions oI HDI. and subfractions with ApoAl were significantly in period A but in B we have not found correlation between ItDI-2 and ApoAI perhaps by a more selective effect of oleic acid m IIDL-3 suhfraction, well correlated with ApoAllp 0.01
169
171
PLASMA PEROX|DATION AND ~-TOCOPHEROL LEVELS IN WOMEN FED DIFFERENT MONOENE OILS" C. Cuesta ~, F.J. SS.nchez-Muniz ~, S. R6denas:, R. Muggli 3, qnstituto de Nutrici6n y Bromatologia (CSIC-UCM). ~'Dpto. Quimica Analitica; ~2Facultad de Farmacia, Universidad Complutense de Madrid (Spain); JHoffmann-La Roche, Basel (Switzerland)
INFLUENCE OF DIETETIC LINOLEIC VERSUS OLEIC FATTY ACID IN LDL-c/APOB RATIO J Millan. A Mangas, [ Cortes, P Conthe Department ot Internal Medicine Hosp General Universit "Gregorio Marafion". Madrid and University of Cadiz
Plasma level of o~-tocopherol were described to protect LDL peroxidation. Also a low plasma level of ~-tocopherol and ascorbate has been indentified as a risk factor in CHD. The purpose of this study was to examine the dietary effects of three monoene oils: virgin olive oil (OO), high oleic sunflower oil (HOSO) and palm olein (PO) with unsaturation index of 0.90, 1,01 and 0.66. respectively on plasma peroxidation and atocopherol levels in 15 women aged I60.2 _4- 14.7 yr). Such oils were used as the only culinary fats during three periods of one month each, representing ~ 62% of the total lipids ingested. The results suggest that in the HOSO period the level of total plasma peroxidation was higher (0.93_+0.67 ftg/mL MDA) than in OO (0.85+_0.70 #g/mL MDA) and PO (0.48 _+ 0.25 #g/mL MDA/ periods despite the higher content of ee-tocopherol in the oils (540, 190 and 100 mg/Kg, respectively) and plasma c~-tocopherol concentrations ( 14.69, 12.31 and 13.17 mg/L, respectively). "Supported by C1CYT (Spain) Project ALl 92-0289-C02-0l and by Hoffmann-La Roche. Basel (Switzerland'~
LDL c/ApoB ratios were evaluated m 5 communities (40 healthy women) in three dietetic conditions according with different oils used ill the diet Changes m diet only affecting to PUFA or MUFA apported, without variations in caloric intake, corporal weight, protein carbohydrate or saturated fatty acid composition. In a two months basal conditions, a mixture of olive oil and sunflower oil was used, l\Hlowed by a three months with 7,8~ of PUFA and 15,4% of MUFA, that was fi~llowed by a four mouths with 5,7% of PUFA and 18,2~f of MUFA. LDL-c/ApoB ratio in basal conditions was 1,78 with an increase II.98) of +11,1% during "sunflower oil period", and a decrease (1,88) of - 5 , 1 % during "olive oil period". These results show variations m LDL-c/ApoB in relation with dietetic modifications m fatty acids intake. In our experience, LDLc/ApoB as an index of density of LDL particles show a beneficious change during the use of olive oil lenriched with oleic fatty acid in the diet. even in small quantity,
$47
170 PROTECTIVE EFFECT OF VITAMIN E ON AORTIC LIPID PEROXIDAT1ON AND ATHEROMA FORMATION IN HYPERCHOLESTEROLEMIC RABBITS L. Kazdov& D. Urbanovzl, A. Vr~ina, M. Mat~j~kov~i, V. Nowikov~i Institute for Clinical and Experimental Medicine, Prague, Czech Republic Free radical-mediated reactions, including lipid peroxidation, are considered to be important factors in the development of atherosclerosis. Therefore, the effect of antioxidant therapy with vitamin E on aortic tissue lipid peroxidation and atherosclerotic lesions development was investigated. Male New Zealand White rabbits were fed a chow diet (C) or atherogenic diet tAD, 0.5% of cholesterol) without or with vitamin E supplementation t A D + E , 60 mg/kg b.wt.) for 8 weeks. To produce more advanced lesions, in~nune-mediated endothelial injury was induced by i.v. injection of 250 mg/kg b.wt. of bovine serum albumin at the beginning of the experiment. We found that vitamin E supplementation had no effect on serum triglycerides tAD:2.3 _+ 0.5 vs AD+E:2.0 _+ 0.5 mmol/I n.s.) or hypercholesterolemia (AD:13.5 ::t 1.9 vs AD+E: 10.5 5 : 2 . 0 nunol/l n.s.). On the other hand, vitamin E decreased aortic tissue malondialdehyde levels tAD:0.29 _+ 0.03 vs AD+E:0.91 _+ 0.08 nunol/mg protein) and reduced aortic cholesterol content by 36% and lesion coverage by 29%. Histology of the lesions revealed lower intimal thickness and decreased foam cell and connective tissue accumulation. In conclusion, results indicate that a) vitamin E inhibited the development of atherosclerosis without lowering hyperlipidemia, b) this effect may be mediated through its antioxidant activity in the artery wall.
CHANGES IN HDL. APOAI. AND HDL SUBFRACTIONS BY REDUCTION OR ADDITION OF DIFFERENT DIETETIC FATTY ACIDS J. Millan', I Cortes, A. Mangas, P. Conthe'. A.Tejedor'. P. GarciaBarreno" Department of Internal Medicine: Hospital General Universitario "Gregorie Marafion" and University of Cadiz In a model of dietetic intervention over 40 women of 5 religious comnmnities, we have evaluated the influence of changes in dietetic afatty acids reduction of oleic acid with adition of linoleic acid, or reductitm ol [inoleic acid with adition of oleic acid without variation in saturated tart) acid fig"; ). carbohydrates (42%) or protein (15%. Caloric intake was not changed aud any change in corporal weight was observed HDL and HDL subfractions (HDL-2 and HDL-3) and correlation with ApoAI were evaluated in two times: period A (light decrease in oleic acid retake -4ml- by reduction of olive oil, with increase -6ml of linoleic acid by aditiun of sunflower oil) and period B (notorious reduction ol linoleic acid -22ml- with increase -20ml- of oleic acid intakel. Ratios {*l variation in biochemical parameters were more well correlated with the ml of fatty acid aditiones. MI of linoleic acid increased were correlated with % of variation of HDL {r -0,681 HDI.-2 i r - 0.74). HDL-3 (r~0.71) and ApoAI (r=-0.76) MI of o]eic acid increased were correlated with % of variation in HDL !r 0,75) HDL-2 {r~-0,51), HDL-3 i r - 0 . 7 4 ) and ApoAI ( r - 0 , 7 6 ) Correlalions oI HDI. and subfractions with ApoAl were significantly in period A but in B we have not found correlation between ItDI-2 and ApoAI perhaps by a more selective effect of oleic acid m IIDL-3 suhfraction, well correlated with ApoAllp 0.01
169
171
PLASMA PEROX|DATION AND ~-TOCOPHEROL LEVELS IN WOMEN FED DIFFERENT MONOENE OILS" C. Cuesta ~, F.J. SS.nchez-Muniz ~, S. R6denas:, R. Muggli 3, qnstituto de Nutrici6n y Bromatologia (CSIC-UCM). ~'Dpto. Quimica Analitica; ~2Facultad de Farmacia, Universidad Complutense de Madrid (Spain); JHoffmann-La Roche, Basel (Switzerland)
INFLUENCE OF DIETETIC LINOLEIC VERSUS OLEIC FATTY ACID IN LDL-c/APOB RATIO J Millan. A Mangas, [ Cortes, P Conthe Department ot Internal Medicine Hosp General Universit "Gregorio Marafion". Madrid and University of Cadiz
Plasma level of o~-tocopherol were described to protect LDL peroxidation. Also a low plasma level of ~-tocopherol and ascorbate has been indentified as a risk factor in CHD. The purpose of this study was to examine the dietary effects of three monoene oils: virgin olive oil (OO), high oleic sunflower oil (HOSO) and palm olein (PO) with unsaturation index of 0.90, 1,01 and 0.66. respectively on plasma peroxidation and atocopherol levels in 15 women aged I60.2 _4- 14.7 yr). Such oils were used as the only culinary fats during three periods of one month each, representing ~ 62% of the total lipids ingested. The results suggest that in the HOSO period the level of total plasma peroxidation was higher (0.93_+0.67 ftg/mL MDA) than in OO (0.85+_0.70 #g/mL MDA) and PO (0.48 _+ 0.25 #g/mL MDA/ periods despite the higher content of ee-tocopherol in the oils (540, 190 and 100 mg/Kg, respectively) and plasma c~-tocopherol concentrations ( 14.69, 12.31 and 13.17 mg/L, respectively). "Supported by C1CYT (Spain) Project ALl 92-0289-C02-0l and by Hoffmann-La Roche. Basel (Switzerland'~
LDL c/ApoB ratios were evaluated m 5 communities (40 healthy women) in three dietetic conditions according with different oils used ill the diet Changes m diet only affecting to PUFA or MUFA apported, without variations in caloric intake, corporal weight, protein carbohydrate or saturated fatty acid composition. In a two months basal conditions, a mixture of olive oil and sunflower oil was used, l\Hlowed by a three months with 7,8~ of PUFA and 15,4% of MUFA, that was fi~llowed by a four mouths with 5,7% of PUFA and 18,2~f of MUFA. LDL-c/ApoB ratio in basal conditions was 1,78 with an increase II.98) of +11,1% during "sunflower oil period", and a decrease (1,88) of - 5 , 1 % during "olive oil period". These results show variations m LDL-c/ApoB in relation with dietetic modifications m fatty acids intake. In our experience, LDLc/ApoB as an index of density of LDL particles show a beneficious change during the use of olive oil lenriched with oleic fatty acid in the diet. even in small quantity,