- Email: [email protected]
Influence of palm oil and palm vitamin E on bone mineral density in orchidectomised male rats
214
Poster Session 4C. Reproductive Toxicology
tion respectivelly. Serum testosterone levels were markedly increased in a highly significant manner in both first and second group. While serum estrogen levels were significantly increased only in the first group. The pathological examination of testes revealed congestion of the blood vessels, intertubular oedema, fibroblastic proliferation followed by leydig cell hyperplasia, and hypertrophy. Degeneration of spermatogenic cells and atrophy of the semineferous tubules were noticed at the end of the experiment specially in the first group. The epidydmis of the treated groups were empty from sperms and showed degeneration of their epithelium. The pathogenesis of these changes was discussed in relation to the hormonal changes. Our results concluded that Nuvacron affect on the semineferous tubules either directly on the spermatogenic cells or indirectly through its effect on the blood vessel walls, intertubular tissue and on the liver.
IP4C51 I
ATRAZINE TOXICITY IN MALE RATREPRODUCTIVE PROCESSES
J. Kniewald *, A. Tomljenovic, M. Jakominic, P. Romac", OJ. Vrandi&, Z. Kniewald. FacultyofFoodTechnology and Biotechnology, UniversityofZagreb, J University Clinic Vuk Vrhovec; 2ClinicalHospital Dubrava, Zagreb, Croatia Herbicide atrazine (A) effect on reproductive processes in male rat has been examined at anterior pituitary (AP), testes (T) , prostate (P) and seminal vesicles (SV). In the present study A in corn oil was administered to 90 days old rats i.p. at 120 mglkg b.w.leach 72 h/60 days. Exposure of rats had induced changes in LH and FSH synthesis. changes of spermatogenesi s and structural changes at all selected tissues. LH and FSH were detected by immunohistochemical method, structural changes by light and electron microscopy, while spermatogenesis has been followed by measuring concentration, total number, motility and progressive motility of sperms, A-males in AP have decreased number of immunoreactive cells for LH 25%, while FSH increased for 70%. In T control FSH is dominantly present on the periphery and is concentrated with the spennatocytes and spermatogonia, In A-rats FSH presence is within the whole irregular organized seminiferous tubules. Histochemistry of P tissue shows the tubular alveolar gland with large irregular cavities in A-rats. Our previous research on OHT-receplOrsin prostate showed the reduction of OHT specific binding sites on receptor molecules. At SV evidently mucosal folds are more tightly packed in A-rats vs. contro!. The weight of SV in A-rats was much lower and a typical atrophy of the gland is present. Inhibition of spermatogene sis in A-treated rats was in sperm count from 14.3 x 106 per ml vs. 17.06 x 106 in controls. At the same time the motile sperm count has been reduced from 43% or 35%, respectively. Supported by Ministry of S&T Republic of Croatia (grant 058104) and PLIVA d.d.
IP4C52 I NaF MAYDISTURBMALE FERTILITY IN RODENTS R. Pinto *, C. Vieira, H. Mota-Filipe, B. Silva-Lima. Lab.
Pharmacology. Fac. Pharmacy. University ofLisbon, Potugal
An increase in the potential for lipoperoxidation in the rat testis and a decrease in rodents fertility by NaF has been reported (Pinto et al., 1997, Phann. Toxicol., 80, Ill, 117; Chinoy et al., 1992, J. Environ. Bioi 13, 55). However, other Authors described that spermatogenesis in the rat is not affected by fluoride (Sprando et al., 1996, Food Chern. Toxico!., 34, 377). In order to contribute to the clarification the effects of NaF in animal fertility we have assessed: I) The effect of fluoride on spermatozoa (spenn .) motility, epididymis (Epidid.) and seminal vesicles (sem,ves.) weight and fructose (fruc.) levels in sem. ves., in mice after 28 days of treatment with 10 mglkg/day of Naf,. 2) Effect of 1 mglkgldayand 10 mglkg/day (Groups FI and F2, respectively) of NaF repeated treatment for 28 days in rat on sperm,
count, epidid., sem. Yes. and testis weight, fruc, levels in sem. ves. and testosterone (testost.) levels. Results: I) In the NaF- treated mice the sperm. motility was modified (vs control), showing circle movements of the flagel without displacement while in the controls a rectilinear displacement occurred, similarly to that of normal Human sperm, The parameters further analised where (p < 0.01) Body-weight (g) start-end: 31 ± 1 - 36 ± 0.4" (control), 32 ± 0.8 - 35 ± I (NaF). Sem. ves. weight (mg): 291 ± 10 (control), 196 ± 11" (NaF). Epidid. weight (mg): 74 ± 5 (control). 74 ± 7 (NaF). Fmc . levels (J!MlIOOmg): 2 ± 0.1 (control), 3 ± 0.2" (NaF). 2) In rats the parameters analised where (p vs control < 0.05) Body-weight gain (g): 69.8 ± 3.2 (Control), 102.5 ± 12.5" (FI), 58.3 ± 4.0" (F2); testis weight (g): 1.77 ± 0.05 (Control), 1.66 ± 0.02" (FI ), 1.63 ± 0.03" (F2); Epidid. weight (g): 0.35 ± 0.01 (Control), 0.30 ± 0.01" (FI ), 0.29 ± 0.Ql" (F2); Sem. ves. weight (g): 0.80 ± 0.03 (Control). 0.57 ± 0.05" (FI), 0.71 ± 0.02" (F2); Testost. Level: (ng/ml): 2.7 ± 0.4 (Control), 2.2 ± 0.8 (FI), 4.3 ± 0.6" (F2); Fruc, level (J!MllOO mg):0.56 ± 0.08 (Control). 0.55 ± 0.11 (FI), 0.38 ± 0.01" (F2). Spenn .count was not modified in NaF-treated groups. Conclusions: The modification of some parameters related to fertility by the repeated oral NaF intake, in rodents, suggest that NaF has potential to disturb male fertility.
IP4C53 I
INFLUENCE OF PALMOIL AND PALMVITAMIN E ON BONE MINERALDENSITY IN ORCHIDECTOMISED MALE RATS
S.lma-Nirwana *, A. Kiftiah, A.G. Zainal 1 , M. Norazlina, M.T. Gapor', B.A.K. Khalid' . Depts. Of Pharmacology and JMedicine, FacultyofMedicine, University Ke~saan Malaysia,
lin. Raja Muda AM Aziz. 50300 Kuala Lumpur; PalmOil ResearchInstitute ofMalaysia, 6, Persiaran Institusi, 43650 Bandar Baru Bangi, Selangot; Malaysia The objective of our study was to observe the effects of long-term supplementation of palm oil and vitamin E-rich extract from palm oil on bone mineral density (BMO) in testosterone-deficient male rats. 3 month-old male Wistar rats were sham-operated (SH) or orchidectomised (ORX) ; and fed rat chow only (control) or supplemented with palm vitamin E 30 mglkg rat weight or 20% palm oil in rat chow for 8 months. BMO was measured using the Dual-Energy X-ray Absorptiometer. Skeletal regions scanned were whole body; whole, proximal, midshaft and distal femora; and lumbar vertebrae. Whole body BMD was higher in the ORX group supplemented with palm vitamin E compared to palm oil. BMO of the whole femur was higher in the SH control group compared to the ORX group supplemented with palm oil. In the proximal femur, BMO was higher in the SH control compared to the ORX control group and the palm oil supplemented group. Supplementation with palm vitamin E maintained the BMD at SH control levels. No significant changes were seen in the midshaft and distal femur and the lumbar vertebrae. In conclusion, significant decline in BMO due to testosterone deficiency in male rats was best seen in the proximal femur, the site of predominantly cortical bone. Palm vitamin E was effective in long-term maintainance of BMD in the proximal femur of testosterone-deficient male rats, while palm oil was not.
IP4C54 I EFFECTS OF CONTROLLED TOLUENE EXPOSURE ON GONADOTROPIN SECRETION IN MENAND WOMEN
U. Luderer *, O.A. Kalman, M.S. Morgan, E.M. Faustman. Departmentof Environmental Health, University of Washington, Seattle, WA, USA
Background: Occupational exposure to the solvent toluene has been
Poster Session 4C. Reproductive Toxicology
tion respectivelly. Serum testosterone levels were markedly increased in a highly significant manner in both first and second group. While serum estrogen levels were significantly increased only in the first group. The pathological examination of testes revealed congestion of the blood vessels, intertubular oedema, fibroblastic proliferation followed by leydig cell hyperplasia, and hypertrophy. Degeneration of spermatogenic cells and atrophy of the semineferous tubules were noticed at the end of the experiment specially in the first group. The epidydmis of the treated groups were empty from sperms and showed degeneration of their epithelium. The pathogenesis of these changes was discussed in relation to the hormonal changes. Our results concluded that Nuvacron affect on the semineferous tubules either directly on the spermatogenic cells or indirectly through its effect on the blood vessel walls, intertubular tissue and on the liver.
IP4C51 I
ATRAZINE TOXICITY IN MALE RATREPRODUCTIVE PROCESSES
J. Kniewald *, A. Tomljenovic, M. Jakominic, P. Romac", OJ. Vrandi&, Z. Kniewald. FacultyofFoodTechnology and Biotechnology, UniversityofZagreb, J University Clinic Vuk Vrhovec; 2ClinicalHospital Dubrava, Zagreb, Croatia Herbicide atrazine (A) effect on reproductive processes in male rat has been examined at anterior pituitary (AP), testes (T) , prostate (P) and seminal vesicles (SV). In the present study A in corn oil was administered to 90 days old rats i.p. at 120 mglkg b.w.leach 72 h/60 days. Exposure of rats had induced changes in LH and FSH synthesis. changes of spermatogenesi s and structural changes at all selected tissues. LH and FSH were detected by immunohistochemical method, structural changes by light and electron microscopy, while spermatogenesis has been followed by measuring concentration, total number, motility and progressive motility of sperms, A-males in AP have decreased number of immunoreactive cells for LH 25%, while FSH increased for 70%. In T control FSH is dominantly present on the periphery and is concentrated with the spennatocytes and spermatogonia, In A-rats FSH presence is within the whole irregular organized seminiferous tubules. Histochemistry of P tissue shows the tubular alveolar gland with large irregular cavities in A-rats. Our previous research on OHT-receplOrsin prostate showed the reduction of OHT specific binding sites on receptor molecules. At SV evidently mucosal folds are more tightly packed in A-rats vs. contro!. The weight of SV in A-rats was much lower and a typical atrophy of the gland is present. Inhibition of spermatogene sis in A-treated rats was in sperm count from 14.3 x 106 per ml vs. 17.06 x 106 in controls. At the same time the motile sperm count has been reduced from 43% or 35%, respectively. Supported by Ministry of S&T Republic of Croatia (grant 058104) and PLIVA d.d.
IP4C52 I NaF MAYDISTURBMALE FERTILITY IN RODENTS R. Pinto *, C. Vieira, H. Mota-Filipe, B. Silva-Lima. Lab.
Pharmacology. Fac. Pharmacy. University ofLisbon, Potugal
An increase in the potential for lipoperoxidation in the rat testis and a decrease in rodents fertility by NaF has been reported (Pinto et al., 1997, Phann. Toxicol., 80, Ill, 117; Chinoy et al., 1992, J. Environ. Bioi 13, 55). However, other Authors described that spermatogenesis in the rat is not affected by fluoride (Sprando et al., 1996, Food Chern. Toxico!., 34, 377). In order to contribute to the clarification the effects of NaF in animal fertility we have assessed: I) The effect of fluoride on spermatozoa (spenn .) motility, epididymis (Epidid.) and seminal vesicles (sem,ves.) weight and fructose (fruc.) levels in sem. ves., in mice after 28 days of treatment with 10 mglkg/day of Naf,. 2) Effect of 1 mglkgldayand 10 mglkg/day (Groups FI and F2, respectively) of NaF repeated treatment for 28 days in rat on sperm,
count, epidid., sem. Yes. and testis weight, fruc, levels in sem. ves. and testosterone (testost.) levels. Results: I) In the NaF- treated mice the sperm. motility was modified (vs control), showing circle movements of the flagel without displacement while in the controls a rectilinear displacement occurred, similarly to that of normal Human sperm, The parameters further analised where (p < 0.01) Body-weight (g) start-end: 31 ± 1 - 36 ± 0.4" (control), 32 ± 0.8 - 35 ± I (NaF). Sem. ves. weight (mg): 291 ± 10 (control), 196 ± 11" (NaF). Epidid. weight (mg): 74 ± 5 (control). 74 ± 7 (NaF). Fmc . levels (J!MlIOOmg): 2 ± 0.1 (control), 3 ± 0.2" (NaF). 2) In rats the parameters analised where (p vs control < 0.05) Body-weight gain (g): 69.8 ± 3.2 (Control), 102.5 ± 12.5" (FI), 58.3 ± 4.0" (F2); testis weight (g): 1.77 ± 0.05 (Control), 1.66 ± 0.02" (FI ), 1.63 ± 0.03" (F2); Epidid. weight (g): 0.35 ± 0.01 (Control), 0.30 ± 0.01" (FI ), 0.29 ± 0.Ql" (F2); Sem. ves. weight (g): 0.80 ± 0.03 (Control). 0.57 ± 0.05" (FI), 0.71 ± 0.02" (F2); Testost. Level: (ng/ml): 2.7 ± 0.4 (Control), 2.2 ± 0.8 (FI), 4.3 ± 0.6" (F2); Fruc, level (J!MllOO mg):0.56 ± 0.08 (Control). 0.55 ± 0.11 (FI), 0.38 ± 0.01" (F2). Spenn .count was not modified in NaF-treated groups. Conclusions: The modification of some parameters related to fertility by the repeated oral NaF intake, in rodents, suggest that NaF has potential to disturb male fertility.
IP4C53 I
INFLUENCE OF PALMOIL AND PALMVITAMIN E ON BONE MINERALDENSITY IN ORCHIDECTOMISED MALE RATS
S.lma-Nirwana *, A. Kiftiah, A.G. Zainal 1 , M. Norazlina, M.T. Gapor', B.A.K. Khalid' . Depts. Of Pharmacology and JMedicine, FacultyofMedicine, University Ke~saan Malaysia,
lin. Raja Muda AM Aziz. 50300 Kuala Lumpur; PalmOil ResearchInstitute ofMalaysia, 6, Persiaran Institusi, 43650 Bandar Baru Bangi, Selangot; Malaysia The objective of our study was to observe the effects of long-term supplementation of palm oil and vitamin E-rich extract from palm oil on bone mineral density (BMO) in testosterone-deficient male rats. 3 month-old male Wistar rats were sham-operated (SH) or orchidectomised (ORX) ; and fed rat chow only (control) or supplemented with palm vitamin E 30 mglkg rat weight or 20% palm oil in rat chow for 8 months. BMO was measured using the Dual-Energy X-ray Absorptiometer. Skeletal regions scanned were whole body; whole, proximal, midshaft and distal femora; and lumbar vertebrae. Whole body BMD was higher in the ORX group supplemented with palm vitamin E compared to palm oil. BMO of the whole femur was higher in the SH control group compared to the ORX group supplemented with palm oil. In the proximal femur, BMO was higher in the SH control compared to the ORX control group and the palm oil supplemented group. Supplementation with palm vitamin E maintained the BMD at SH control levels. No significant changes were seen in the midshaft and distal femur and the lumbar vertebrae. In conclusion, significant decline in BMO due to testosterone deficiency in male rats was best seen in the proximal femur, the site of predominantly cortical bone. Palm vitamin E was effective in long-term maintainance of BMD in the proximal femur of testosterone-deficient male rats, while palm oil was not.
IP4C54 I EFFECTS OF CONTROLLED TOLUENE EXPOSURE ON GONADOTROPIN SECRETION IN MENAND WOMEN
U. Luderer *, O.A. Kalman, M.S. Morgan, E.M. Faustman. Departmentof Environmental Health, University of Washington, Seattle, WA, USA
Background: Occupational exposure to the solvent toluene has been