Influence of some serum parameters on pseudocholinesterase levels

C h e m o s p h e r e , Vol.17, No.9, P r i n t e d in Great B r i t a i n

pD 1871-1874,

1988

0 0 4 5 - 6 5 3 5 / 8 8 $3.00 + .OO P e r g a m o n Press plc

INFLUENCE OF SOME SERUM PARAMETERS ON PSEUDOCHOLINESTERASE J. A. Garcfa-L6pez*; Unidad de Bioqufmica.

LEVELS.

M. A. Garcfa-L6pez and ~I. Nonteoliva.

Instituto "L6pez Neyra" de Parasitologfa.

C/ Real N 2 53. Valderrubio.

Granada.

C.S.[.C.

Espa~a.

ABSTRACT We carried out a study of the possible linear relationship between ChE activity and serum parameters indicating certain pathological states, such as glucose, urea, uric acid, total bilirubin, creatinine, TC, TG, HDL-C, LDL-C, SGOT, SGPT, ~-GT, alkaline phosphatase, amylase activities and the SGOT/SGPT, ~-GT/SGOT and ~-GT/SGPT enzymatic ratios. A significant linear relationship (p,=O.05) was obtained between ChE and parameters such as SGOT, SGPT and alkaline phosphatase. We discuss wheter ChE activity is specific evidence of certain hepatic pathologies or simply an indicator of the general synthetic capacity of the liver.

INTRODUCTION Pseudocholinesterase of

choline

esters

glucoprotein

with

(E.C.I.I.8) and

even

(ChE)

other

is an enzyme capable

estecs

a molecular weight

such

as

benzoic

several

hypotheses

have been suggested,

still unknown. For some authors, conduction lipids is

nerves

Other

in intestinal

activity

is

not

ChE

is

a

the main physiological

function of CnE is

ChE plays a vital role in the nervous transmission of slow authors

motility

detected

derivatives.

(i).

claim

that

it

is

involved

(3), that it plays a regulating role in choline homeostasis

involved

ChE

(2).

acid

a large numDer

of between 300000 and 365000 and an average life of

to 12 days which is found in plasma and many other tissues Although

of hydrolysing

(5).

does

not

The fact that support

the

in

the

in plasma

there are normal

functions

metabolism

subjects

suggested

of

(4) or that it

for

in which

the enzyme,

although an alternative mechanism may exist in these subjects. ChE serum found

levels have been studied

in hyperlipoproteinemic,

schizophrenia, patients

hypertension

suffering

from

and

paper

studies

certain pathologies,

the

patients,

alcoholism.

malnutrition,

infarction and acute infections This

in many pathologies

hyperthyroid

Low

and high activity levels have been and in cases of obesity,

activity

hepatic

values

disturbances,

have

been

carcinomas,

diabetes,

observed

in

myocardial

(6).

influence

of

the

level

of

some

serum

parameters,

on ChE activity with the aim of clarifying

for the clinical diagnosis.

1871

which

indicate

the utility of the enzyme

1872

14ATERIAL AND METIIODS A total of 134 blood samples were analysed

from subjects

69 women, who came to our clinical analysis laboratory. method

of

venipuncture,

poured

into

hemolysis

over 25 years of age, 65 men and

Blood is extracted by the classical

tubes

and,

after

centrifugation,

serum is

separated. Serum parameters total

related

cholesterol

lipoproteins

(TC),

(LDL-C),

glutamic-pyruvic (AP)

and

These

method

substrate

were

Ellman

nitrobenzoic

per

activities

commercial

of

total

and

acid

and

at 37°C.

litre of serum

in a Beckman methods,

(1961)

thiocholine

Activity (U/L).

glutamic-oxaloacetic

the SGOT/SGPT,

(work

coll.

high-density lipoproteins

(TB),

(8GPT), ~-glutamyl and

analysed

test

(TG),

bilirubin

transaminase

amylase

parameters

Mannheim

to ChE activity were: glucose level, urea, uric acid, creatinine, triglycerides

(7)

based

Serum

on in

the

ChE

alkaline

measurements

(SGOT),

phosphatase

enzymatic

was

between

hydrolysis

of substrate

low-density

ratios.

using the Boehringer

activity

reaction

enzymatic

in micromoles

All anaytical

(~-GT),

and ~-GT/SGPT

DB-G spectrophotometer,

1982).

released

is given

transferase ~-GT/SGOT

(HDL-C),

transaminase

analysed

5-5' of

by

the

dithio-bJs-2-

buty lthiocholine

hydrolized per minute and

were done within 8 hours after blood

extraction. The

statistical

separately

in

method men

and

used women

was

the

since

correlation there

was

signEficantly different values of AChE activity

coefficient clear

test.

evidence

The

that

test was applied

both

groups

showed

(8).

RESULTS TABLE i. CORRELATION BETWEEN CHE AND SOME SERUM PARAMETERS In men

In women 5

r

Glucose

mg/dl

65

114.03

32.826

0.244

NS

64

106.92

37.562

0.241

N8

Urea

mg/dl

43

37.09

7.914

-0.135

NS

46

34.69

7.865

0.096

NS

mg/dl

33

5.54

1.617

-0.035

NS

40

4.48

1.432

0.063

N8

0.86

0.184

-0.002

NS

N

Parameter

Uric

acid

Creatinine mg/dl

S

r

p

N

23

0.97

0.178

0.072

NS

13

~

p

TC

mg/dl

63

218.50

40.342

0.027

NS

69

210.27

40.461

0.180

NS

TG

mg/dl

53

161.69

70.912

0.267

NS

59

123.18

73.064

0.198

NS

HDL-C

mg/dl

39

44.33

11.501

-0.108

NS

48

53.50

15.671

-0.020

NS

LDL-C

mg/dl

37

145.32

33.127

-0.206

N8

45

137,86

37.473

0.126

NS

TB

mg/dl

38

0.85

0.577

-0.257

NS

33

0.65

0.349

-0.052

NS

SGOT

U/L

53

33.45

33.987

-0.430

p
48

23.37

12.555

0.074

NS

8GPT

U/L

53

35.79

'28.228

-0.390

p
48

25.39

21.445

0.288

p~O.05

~-GT

U/L

42

68.50

179.579

-0.250

NS

25

23.44

15.248

0.125

NS

SGOT/SGPT

53

1.03

0.412

-0.260

NS

48

1.13

0.473

-0.400

p
~-GT/SGOT

42

1.65

1.558

0.027

NS

24

0.92

0.420

0.439

p
~-GT/SGPT

42

1.83

2.410

-0.183

N8

24

1.03

0.515

0.019

NS

p
30

203.36

144.610

0.353

NS

NS

11

161.00

105.527

0.421

N8

69

5.01

1.640

AP

UIL

45

208.95

144.201

-0.310

Amylase

U/L

15

136.00

50.548

0.051

Chg

U/L

65

5.20

1.228

N: number of samples; ~: arithmetic mean; S: typical deviation; p: significance; NS: non-significant.

r: correlation coefficient;

1873

Table 1 shows mean values for each of the parameters analyzed and the results of the linear correlation analysis between ChE activity and other serum parameters. In the case of men there is a significant linear relationship between ChE activity and SGOT, SGPT and alkaline phosphatase activities. In the case of women there is a significant linear relationship

with

SGPT

activity

and

the

SGOT/SGPT

and

~-GT/SGOT

enzymatic

ratios.

No

related

to

significant relationships are obtained with other parameters correlated.

DISCUSSION The

results

obtained

show

that

the

values

of

some

serum

parameters

may

be

ChE activity. Although such

as

no

significant

glucose

and

both in men and women, of

this size,

linear

relationship was obtained between ChE and serum parameters

triglycerides,

the

obtained

for

the correlation coefficient,

are very close to those required to become significant for a sample

and which would justify

hypertriglyceridemia

results

patients

the high values of ChE activity found in diabetic or

whereas

no

significant

changes

were

found

in

patients

suffering from hypercholesterinemia (3). No significant linear relationship, in both men and women, is found between ChE activity and serum

levels

of

urea,

uric

acid,

creatinine,

total

cholesterol,

HDL-cholesterol,

LDL-cholesterol, total bilirubin and ~-GT, amylase activities and ~-GT/SGPT enzymatic ratio, although

soiILe authors

LDL-cbolesterol,

(9) have

found

HDL-cholesterol,

a

significant

relationship

very-low-density lipoproteins

between ChE activity and

(VLDL-C),

total cholesterol

and triglyceride levels in hypertensive subjects. We found a significant linear relationship ( p < O . O 5 )

between thE activity and SGOT, SGPT and

alkaline phosphatase in men and SGPT, SGOT/SGPT, ~-GT/SGOT activities in women, which proves the utility of the enzyme as a functional hepatic test. The

relationship

inverse

and

obtained

direct

in

the

in

the

case

case

of

of women.

SGPT In

is

the

surprising; case

of

in

women,

the it

case is

of men

logical

it

that

is the

relationship with the SGOT/SGPT ratio should be inverse as, if the relationship with SGPT is direct, when the latter increases, the $GOT/SGPT ratio decreases. These

indicators

relationship for

this

of

with

reason

the hepatic

ChE

we

function with which we have obtained a significant linear

appear at higher levels in obstructive jaundice and cirrhosis

expect

low

ChE activity values for these two hepatic diseases,

(iO); as has

already been pointed out by several authors (i, ii). Although think

in some

that

it

of the general

hepatopathies ChE activity is lower,

is not

specific

taking into account

evidence of some hepatic pathology but

synthetic capacity of the liver and that

clinical utility of the enzyme is expected.

the results, we

rather an indicator

this is the area where the highest

1874

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