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s in hypertensive preeclamptic parturients
250
Pharmacological
Research, Vol. 26, Supplement I, 1992
INHIBITION OF EDRF/NO PRODUCTION IN RABBIT AORTA BY SERUM PURI FIED FROM FACTOR/S IN HYPERTENSIVE PREECLAMPTIC PARTURIENTS A.Pinto,R.Sorrentino,L.Sautebin,P.Martinelli*,T.Guerritore*and L-Sorrentino Pharmacology, Faculty of Pharmacy and *Inst. Dept. Experimental of Gynaecology, Obstetrics and Physiology of Human Reproduction -11 Faculty of Medicine and Surgery, University of Naples "Federico II" In previous work we showed a marked reduction of EDRF/NO from umbilical endothelial cells of pregnancy-induced hypertensive parturients. This results let us to hypotize a generalized reduction of EDRF/NO production in the mother circulation, possi ble due to the presence of an endogenous inhibitory factor/s.To test this hypthesis we have fractionated, by gel filtration (Sephadex G 200), the serum of preeclamptic parturients and found two fractions named F, and F6 that significantly inhibited (56.6+8.5% and 72.656.3s respectively, p 0.01) NO generation mediated by ACh (0.01-l uM) in rabbit aorta rings-The apparent m-w. of F was greater than 400 kd and the m-w. of F was minor than 1 b kd. Further studies are in progress to be Eter characterize these inhibitory factors.
GAMMA-(GLUTAMYL5)-SPERMINE DERIVATIVE OF SUBSTANCE P RETAINS ONLY NITRIC OXIDE MEDIATED BIOLCGICAL ACTIVITIES P. Persiq, A. CaliqIano, L. Sorrentino, L. F. Mancuq, 6r R.,Porta Mariniello , P. Gentile and Dept. of Biochem. and Biophys., Dept. of Exp. Pharmacol. University of Naples l*Federico II". Substance P (SP) is an undecapeptide, released from with a variety of effects on both stimulated sensory fibres, we report neural and nonneural tissues. In the present stud s )-spermine y-(glutamyl the some biological activities of the Spm-SP was synthesized h derivative of substance P (Spm-SP). vitro by the use of the enzyme transglutaminase (1). Spm-SP inhibited collageninduced relaxation of aorta rings, mediated platelet aggregation and induced only a slight inflammatory response in the rat hind paw failed to contract the isolated rat stomach strip and duodenum. Since the antagonist of nitric oxide biosynthesis, L-NAME effectively we conclude that Spm-SP retains counteracted Spm-SP effects, to receptor(s) only the specific property of SP to bind involved in the transduction for the signal of nitric oxide release. C. Esposito, S. Metafora, P. Pucci, A. Malorni 1) R. Porta, and G. Marino, Anal. Biochem. 172, 499, 1988.
Pharmacological
Research, Vol. 26, Supplement I, 1992
INHIBITION OF EDRF/NO PRODUCTION IN RABBIT AORTA BY SERUM PURI FIED FROM FACTOR/S IN HYPERTENSIVE PREECLAMPTIC PARTURIENTS A.Pinto,R.Sorrentino,L.Sautebin,P.Martinelli*,T.Guerritore*and L-Sorrentino Pharmacology, Faculty of Pharmacy and *Inst. Dept. Experimental of Gynaecology, Obstetrics and Physiology of Human Reproduction -11 Faculty of Medicine and Surgery, University of Naples "Federico II" In previous work we showed a marked reduction of EDRF/NO from umbilical endothelial cells of pregnancy-induced hypertensive parturients. This results let us to hypotize a generalized reduction of EDRF/NO production in the mother circulation, possi ble due to the presence of an endogenous inhibitory factor/s.To test this hypthesis we have fractionated, by gel filtration (Sephadex G 200), the serum of preeclamptic parturients and found two fractions named F, and F6 that significantly inhibited (56.6+8.5% and 72.656.3s respectively, p 0.01) NO generation mediated by ACh (0.01-l uM) in rabbit aorta rings-The apparent m-w. of F was greater than 400 kd and the m-w. of F was minor than 1 b kd. Further studies are in progress to be Eter characterize these inhibitory factors.
GAMMA-(GLUTAMYL5)-SPERMINE DERIVATIVE OF SUBSTANCE P RETAINS ONLY NITRIC OXIDE MEDIATED BIOLCGICAL ACTIVITIES P. Persiq, A. CaliqIano, L. Sorrentino, L. F. Mancuq, 6r R.,Porta Mariniello , P. Gentile and Dept. of Biochem. and Biophys., Dept. of Exp. Pharmacol. University of Naples l*Federico II". Substance P (SP) is an undecapeptide, released from with a variety of effects on both stimulated sensory fibres, we report neural and nonneural tissues. In the present stud s )-spermine y-(glutamyl the some biological activities of the Spm-SP was synthesized h derivative of substance P (Spm-SP). vitro by the use of the enzyme transglutaminase (1). Spm-SP inhibited collageninduced relaxation of aorta rings, mediated platelet aggregation and induced only a slight inflammatory response in the rat hind paw failed to contract the isolated rat stomach strip and duodenum. Since the antagonist of nitric oxide biosynthesis, L-NAME effectively we conclude that Spm-SP retains counteracted Spm-SP effects, to receptor(s) only the specific property of SP to bind involved in the transduction for the signal of nitric oxide release. C. Esposito, S. Metafora, P. Pucci, A. Malorni 1) R. Porta, and G. Marino, Anal. Biochem. 172, 499, 1988.