Inhibition of Nematode Development with Thiabendazole*

Preliminary and Short Report INHIBITION OF NEMATODE DEVELOPMENT WITH THIABENDAZOLE* ORVILLE J. STONE, M.D., J. FRED 1VIULLINS, M.D. AND CAROLYN J. WILLIS, M.A. In previous papers (1, 2, 3), the effectiveness of tities. Every plate with thiabendazole was comthiabendazole in "creeping eruption" has been re- pletely negative for the development of larvae. ported, and the other uses of the drug have been CONCLU5ION reviewed. Another report (4) described in detail the observation that this drug has no effect in vitro Thiabendazole produced complete inhibition of on the infective stage of Ancylostoma ceninum lar- development of A. ceninum eggs into infective larvae. After exsheathment by the use of CO2 the or- vae. To our knowledge this is the first report of in ganism did not become susceptible to thiabendazole. vitro activity of thiabendazole against a nematode. It was our opinion that the organism must progress This possibly means that the drug is acting unin its development within the skin to a stage ca- changed in the body and not depending on a metapable of ingesting the drug. It has generally been bohc product. Or it may be possible that metabolic assumed that the organism does not progress be- changes take place in the parasite, rendering it susyond the infective stage within human skin. ceptible to t he drug. Our work was based on the assumption that the drug itself is effective and that metabolism is not SUMMARY required. However, almost all published studies on Thiabcndazole (125 micrograms/ml) completely thiabendazole have been limited to therapy of clinical disease. We know of no previously published inhibited the development of the infectious stage of data on in vitro effectiveness against nematodes. A. caninum from the eggs. The fact that thiabendaThe drug is effective in vitro against the dermato- zole is effective in vitro further substantiates the impression that the organism is susceptible to the phytes (5). ingested drug. The clinical effectiveness of the drug makes it seem likely that the organism progresses METHOD in development within the skin to a stage where it The stools of five dogs with known natural honkwork infection were gathered separately. Each stool ingests the drug. was separately mixed and divided equally into four REFERENCES petri plates, each containing an equal quantity of 1. STONE, 0. J. AND MULLIN5, J. F.: First use of charcoal. Three of the plates from each animal were

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