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Interaction bombesin pentagastrin in acid secretion: Paradoxical effects
IN PENTAGASTRIN IN ACID SECRETION:
L EFFECTS
A. BADO, M. DUBRASQUET, M.J.M. LE~ astric acid secretion and the rele I t was reported that i . v . infusion retion in dog. However i t had bee t r i n , pentagastrin or histamine st cal results, we compared, in the s n Pg stimulated acid secretion. istula (GF) and fundic denervated ximally stimulated by Pg (4 ug/kg/ or 344 pmol) were given i . v . (sing observed 344 pmol BBS was further ~ntal protocol was run with subthreshold dose of carbachol carba (CC) I t s : Bolus injection of 86 pmol of BBS inhibits Pg stimulated st and in HP (37 ± 5% p
us gastrointestinal not modify pentagastrin ed that bolus injection retions in rats. In the cat, the effects of ouch (HP) were used. and on the plateau or 30 min infusion). ng l hr. The same expee Pg infusion. th in GF (47 ± 5% significantly d which lasted for l hr ed in GF by the ce of CC. nhibition of Pg stimuwhen steady state conWe suggest that under ation gradient is r steady state condi-
BOMBESIN-LIKE PEPTIOES: POSSIBLE TUMOR PROMOTING ACTIVITY IN THE RAT PANCREAS. David L. Berry, Ph. ~gional Research Center, USDA, BOO Buchanan Street, Berkeley, Berkel CA 947]0, USA. Food Safety Unit, Western Reg fat or In the multistage, rat pan( ~creatic tumor model, diets containing high levels of unsaturated u Soy f l protein isolates promote carclno arcinogen-initiated tumors to acinar cell adenomas and carcinomas. q and soy protein isolates promote spontaneous adenomas in Wistar rats on IB month mont continuous feedi enha Treatment of weanling rats with soy protein isolates by gavage results in enhanced protein kinasq a c t i v i t y , induction of ornithine ithine decarboxylase a c t i v i t y , increased DNA synthesis and increased mito a c t i v i t y . These responses are dose-dependent and the time course indicates that activation of prot kinase C is an early event t in the mitogenic process in the rat pancreas. Cont Continuous feeding of isolates resulted in hypertrophy, :rophy, hyperplasia, focal areas of dysplasia, nodule formation for and developm ~ositive for GRP whet of tumors. Using immunohistochemi ;tochemical emical techniques, the soy-promoted adenomas were po they were spontaneous or azaserineaserine-initiated. ne-initiated. Nodules at 2B days were positive for GRP which may indic a continuum from nodule to adenoma. The secretory epithelium making up the diffuse neuroendocrine sys in the small intestine was positive for both CCK and GRP. The pancreas was i n i t i a l ly positive in a fo manner for both CCK and GRP; however, only the nodules w e r e diffusely positive for G Azaserine-initiated, unsaturated rated fat-promoted acidophilic nodules, adenomas and carcinomas car were also positive. I t is not currentl ntly known i f the GRP-positive tumors are autocrine in nature or whether t are neuroendocrine stimulated. However, these results indicate that GRP appears to be associated with make i t I l k early promotion of tumors inn the rat pancreas. The strong mitogenic properties of bombesin b that i t is acting as a promoting oting agent in the rat pancreas.
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L EFFECTS
A. BADO, M. DUBRASQUET, M.J.M. LE~ astric acid secretion and the rele I t was reported that i . v . infusion retion in dog. However i t had bee t r i n , pentagastrin or histamine st cal results, we compared, in the s n Pg stimulated acid secretion. istula (GF) and fundic denervated ximally stimulated by Pg (4 ug/kg/ or 344 pmol) were given i . v . (sing observed 344 pmol BBS was further ~ntal protocol was run with subthreshold dose of carbachol carba (CC) I t s : Bolus injection of 86 pmol of BBS inhibits Pg stimulated st and in HP (37 ± 5% p
us gastrointestinal not modify pentagastrin ed that bolus injection retions in rats. In the cat, the effects of ouch (HP) were used. and on the plateau or 30 min infusion). ng l hr. The same expee Pg infusion. th in GF (47 ± 5% significantly d which lasted for l hr ed in GF by the ce of CC. nhibition of Pg stimuwhen steady state conWe suggest that under ation gradient is r steady state condi-
BOMBESIN-LIKE PEPTIOES: POSSIBLE TUMOR PROMOTING ACTIVITY IN THE RAT PANCREAS. David L. Berry, Ph. ~gional Research Center, USDA, BOO Buchanan Street, Berkeley, Berkel CA 947]0, USA. Food Safety Unit, Western Reg fat or In the multistage, rat pan( ~creatic tumor model, diets containing high levels of unsaturated u Soy f l protein isolates promote carclno arcinogen-initiated tumors to acinar cell adenomas and carcinomas. q and soy protein isolates promote spontaneous adenomas in Wistar rats on IB month mont continuous feedi enha Treatment of weanling rats with soy protein isolates by gavage results in enhanced protein kinasq a c t i v i t y , induction of ornithine ithine decarboxylase a c t i v i t y , increased DNA synthesis and increased mito a c t i v i t y . These responses are dose-dependent and the time course indicates that activation of prot kinase C is an early event t in the mitogenic process in the rat pancreas. Cont Continuous feeding of isolates resulted in hypertrophy, :rophy, hyperplasia, focal areas of dysplasia, nodule formation for and developm ~ositive for GRP whet of tumors. Using immunohistochemi ;tochemical emical techniques, the soy-promoted adenomas were po they were spontaneous or azaserineaserine-initiated. ne-initiated. Nodules at 2B days were positive for GRP which may indic a continuum from nodule to adenoma. The secretory epithelium making up the diffuse neuroendocrine sys in the small intestine was positive for both CCK and GRP. The pancreas was i n i t i a l ly positive in a fo manner for both CCK and GRP; however, only the nodules w e r e diffusely positive for G Azaserine-initiated, unsaturated rated fat-promoted acidophilic nodules, adenomas and carcinomas car were also positive. I t is not currentl ntly known i f the GRP-positive tumors are autocrine in nature or whether t are neuroendocrine stimulated. However, these results indicate that GRP appears to be associated with make i t I l k early promotion of tumors inn the rat pancreas. The strong mitogenic properties of bombesin b that i t is acting as a promoting oting agent in the rat pancreas.
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