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Interaction of endothelial cells and the vessel wall
SYMPOSIUM VIII:
BLOOD CELLS - VESSEL WALL INTERACTION THURSDAY, June 5, 1986 Abstract No. 240 - Abstract No. 244
240 PLATELET-VESSEL WALL INTERACTION - NEW DATA T. B. Tschopp, Switzerland Abstract not available at press time. 241 INTERACTION OF ENDOTHELIAL CELLS AND THE VESSEL WALL E. DeJana, Italy Abstract not available at press time. 242 MEGAKARYOCYTE - VESSEL WALL INTERACTION A. Eldor, R. Stromberg, I. Vlodavsky, Y.G. Caine, M. Hersch, L.I.mn and R.F. Levine Dept. Hematology, Hadassah Hospital, Jerusalem, ISRAEL, American Red Cross, Bethesda, Md. 20814, VA Medical Center, Washington D.C. 20422 USA We examined the interactions of megakaryocytes (MK) with subendothelial extracellular matrix (ECM), elaborated by bovine endothelial cells both under static conditions or when subjected to flow in a perfusion system. MK were harvested from guinea pig, rat and human marrow by centrifugal elutriation and velocity sedimentation. When interacted with the ECM, the animal and human MK showed a non reversible adherence, an extensive formation of filopodia and flattening, and under certain conditions many cells underwent total fragmentation and produced platelet-like particles. The effects of flow were examined using guinea pig MK which were circulated for 7 hrs through a parallel plate perfusion chamber which contained two cover slips coated with ECM. Attachment of MK to ECM was monitored by time-lapse video microscopy and SEM. At shear rates of 60-100 set-1, MK adhered to the ECM within 1 minute and the number of attached cells increased for several hrs. MK adhesion to ECM increased as the shear rate was decreased. Many adherent MK underwent elongation and pseudopodia formation resembling MK observed in the vena cava blood. At a low shear rate (10 set-f), MK formed aggregates on the ECM. These findings indicate the remarkable similarity between MK and platelets and suggest that MK attachment to vessel wall components may be important physiologically.
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BLOOD CELLS - VESSEL WALL INTERACTION THURSDAY, June 5, 1986 Abstract No. 240 - Abstract No. 244
240 PLATELET-VESSEL WALL INTERACTION - NEW DATA T. B. Tschopp, Switzerland Abstract not available at press time. 241 INTERACTION OF ENDOTHELIAL CELLS AND THE VESSEL WALL E. DeJana, Italy Abstract not available at press time. 242 MEGAKARYOCYTE - VESSEL WALL INTERACTION A. Eldor, R. Stromberg, I. Vlodavsky, Y.G. Caine, M. Hersch, L.I.mn and R.F. Levine Dept. Hematology, Hadassah Hospital, Jerusalem, ISRAEL, American Red Cross, Bethesda, Md. 20814, VA Medical Center, Washington D.C. 20422 USA We examined the interactions of megakaryocytes (MK) with subendothelial extracellular matrix (ECM), elaborated by bovine endothelial cells both under static conditions or when subjected to flow in a perfusion system. MK were harvested from guinea pig, rat and human marrow by centrifugal elutriation and velocity sedimentation. When interacted with the ECM, the animal and human MK showed a non reversible adherence, an extensive formation of filopodia and flattening, and under certain conditions many cells underwent total fragmentation and produced platelet-like particles. The effects of flow were examined using guinea pig MK which were circulated for 7 hrs through a parallel plate perfusion chamber which contained two cover slips coated with ECM. Attachment of MK to ECM was monitored by time-lapse video microscopy and SEM. At shear rates of 60-100 set-1, MK adhered to the ECM within 1 minute and the number of attached cells increased for several hrs. MK adhesion to ECM increased as the shear rate was decreased. Many adherent MK underwent elongation and pseudopodia formation resembling MK observed in the vena cava blood. At a low shear rate (10 set-f), MK formed aggregates on the ECM. These findings indicate the remarkable similarity between MK and platelets and suggest that MK attachment to vessel wall components may be important physiologically.
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