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Interaction of the isolated chromosomes with the liposomes
Cell Biology International
P5
INTERACTION OF THE ISOLATED CRROMOS@MES WITH THE LIPOSOmS
Nikolai
D Belyaev, Vladimir G Budker, Larr;r A Balakireva, Nika A Dubrovskaya Novosibirsk Institute of Bioorganic Chemistry, Novosibirsk-90, @0090, USSR The interaction of isolated chromosomes from mouse fibroblasts with model mem branes foam stndied. We found that the chromosomes formed complexes with liposomes of lecithin in presence of Ca+2. Formation of the complexes resulted in decon densation of the chromosomes,accompanied with the migration of the protein MW-33kD to the liposomes. Using the same model sy tern we demonstrated the fnsion of the liposomes interacted with chromosomes. The fnsion was detected by electron microscoDy and methods of fluoreseRht spectroscopy. The data suggest that the interaction of the chromosomes with membrane vesicles at the end of mitosis is a key step both in the chromosomal rearrangement and nuclear envelope formation.
CBROIK’SOUALFRAGILE SITES IN PATIENTS WITB LYHPBOnA Yu-luan Wane. Ze-zhonn Tam. Nan Bu*. Shrnxi Cancer lnsiitute, Tiiyuan 030013,“&ncer Institute, Chinese Academy of Medical sicences, Beijing 100021, The Peopleis Republic of China. Chromosomal fragile site studies vere Derforned in 40 patients (31 iles rind 9 fenales, ;ged S-62 years) with lymphoma (12 Eodgkin’s disease, 28 Non-Eodrkin’s lum~hoaa) and 30 individuals (21 males and 3 feilk, aged 7-60 yew) es healthy normal controls. The peripheral blood lymphocytes were harvested by standard procedure for chromosome preparation. 100 metaph8se plates per patient vere analyzed for break and gap. G-banding analyses were carried out after microphotography of aberration in order to specify their precise location and confirm fragile sites. They were located according to ISCN (lSgl), mnd screened out fragile sites according to IIGUS. The results were* 1) The rate of chroeosonal aberration of patients was 16.W the healthy individuals vas 0.9%. There vms e strtisticml diff&ence-b&wee; iie two gromps
P7
Reports, Vol. 14, Abstracts Supplement
1990
SIZE OFMACROMJCLEARDNAMOLEXIJLES OF TETRAHYMENAPYRIFOPMIS Hans-Joachim Horstmann, Institut fiir Biochemie, Universitlt Erlangen, Erlangen, FRG. The DNAof T. pyrifonnis was investigated using pulsed-field gel electrophoresis (CHEF-technique (l), pulse times: 72 s to 60 min, field strength: 1.5 to 4.4 V/cm, running time: 48 to 144 h). The size range of DNAscreened was from 2 kb up to 12 Mb, using amronriate molecular size markers. The gels were ;taiied with ethidium bromide, recorded on Polaroid tvpe . _ 665 film and scanned by densitometry. It was found possible to separate the macronuclear DNAinto 72 discrete bands ranging from 48 kb up to 5.2 Mb. Someotherbands contained up to three times the amount of DNAas neighbouring bands; they are apparently composedof different molecules of similar size. However, two very sharp and intensively stained bands of 70 kb and 144 kb, respectively, possibly representing amplified rDNA and its dimer, contained more than ten times the nmber of DNAmolecules of most of the other bands. A to-1 of about 125 different subchromosomal elements have thus been identified in the macronucleus of Tetrahymena pyriformis. The sum of the DNAof all these individual molecules amounted to about 90 Mb, that is about 43 per cent of the haploid genomeof the micronuclei of this organism. (1) G. Chu, D. Vollrath, R.W.Davis, Science 234, P6
1582-1585
P8
(1986).
NUCLEAR DNA CHANGES WITHIN HELIANTHDs ANNUUS L.
Andrea Cavallini. Lucia Natali, Oria Sassoli, Pier Giorgio Cionini*, Mauro Durante. Dept. Agric.Plant Biol.,University of Pisa and *Dept. Plant Biology, University of Perugia, Italy. In Helianthus annuus, the amount of nuclear DNA continuously varied during reproduction, while the chromosome number and gross structure keep unchanged. Indeed, the results of cytophotometric determinations indicate that the DNA contents differ significantly even in seedlings obtained from seeds collected from different portions of single inflore scences of plants belonging to a selfed line. The variations take place during early embryo development, according to precise mechanisms controlling the variation range, and DNA sequences contained in the heterochromatin are mainly involved. The DNAs extracted from seedlings obtained from seeds collected at peripheral or central portions of an inflorescence were compared. The redundancy of sequences reassociating within C.t values (0.22. 0.22-2.1 and 2.1-100 is higher in the former than in the latter (1.72~105, 5.81.103, 5.26.102 vs. 1.23.105,4.30.103 respectively). These results are conand 2.46.lOi. firmed by slot blottings performed with the same DNA fractions. Moreover, these fractions isolated from the two DNAs show different restriction patterns. The observed nuclear DNA changes are correlated with some growth and phenotypic characters of the plants.
P5
INTERACTION OF THE ISOLATED CRROMOS@MES WITH THE LIPOSOmS
Nikolai
D Belyaev, Vladimir G Budker, Larr;r A Balakireva, Nika A Dubrovskaya Novosibirsk Institute of Bioorganic Chemistry, Novosibirsk-90, @0090, USSR The interaction of isolated chromosomes from mouse fibroblasts with model mem branes foam stndied. We found that the chromosomes formed complexes with liposomes of lecithin in presence of Ca+2. Formation of the complexes resulted in decon densation of the chromosomes,accompanied with the migration of the protein MW-33kD to the liposomes. Using the same model sy tern we demonstrated the fnsion of the liposomes interacted with chromosomes. The fnsion was detected by electron microscoDy and methods of fluoreseRht spectroscopy. The data suggest that the interaction of the chromosomes with membrane vesicles at the end of mitosis is a key step both in the chromosomal rearrangement and nuclear envelope formation.
CBROIK’SOUALFRAGILE SITES IN PATIENTS WITB LYHPBOnA Yu-luan Wane. Ze-zhonn Tam. Nan Bu*. Shrnxi Cancer lnsiitute, Tiiyuan 030013,“&ncer Institute, Chinese Academy of Medical sicences, Beijing 100021, The Peopleis Republic of China. Chromosomal fragile site studies vere Derforned in 40 patients (31 iles rind 9 fenales, ;ged S-62 years) with lymphoma (12 Eodgkin’s disease, 28 Non-Eodrkin’s lum~hoaa) and 30 individuals (21 males and 3 feilk, aged 7-60 yew) es healthy normal controls. The peripheral blood lymphocytes were harvested by standard procedure for chromosome preparation. 100 metaph8se plates per patient vere analyzed for break and gap. G-banding analyses were carried out after microphotography of aberration in order to specify their precise location and confirm fragile sites. They were located according to ISCN (lSgl), mnd screened out fragile sites according to IIGUS. The results were* 1) The rate of chroeosonal aberration of patients was 16.W the healthy individuals vas 0.9%. There vms e strtisticml diff&ence-b&wee; iie two gromps
P7
Reports, Vol. 14, Abstracts Supplement
1990
SIZE OFMACROMJCLEARDNAMOLEXIJLES OF TETRAHYMENAPYRIFOPMIS Hans-Joachim Horstmann, Institut fiir Biochemie, Universitlt Erlangen, Erlangen, FRG. The DNAof T. pyrifonnis was investigated using pulsed-field gel electrophoresis (CHEF-technique (l), pulse times: 72 s to 60 min, field strength: 1.5 to 4.4 V/cm, running time: 48 to 144 h). The size range of DNAscreened was from 2 kb up to 12 Mb, using amronriate molecular size markers. The gels were ;taiied with ethidium bromide, recorded on Polaroid tvpe . _ 665 film and scanned by densitometry. It was found possible to separate the macronuclear DNAinto 72 discrete bands ranging from 48 kb up to 5.2 Mb. Someotherbands contained up to three times the amount of DNAas neighbouring bands; they are apparently composedof different molecules of similar size. However, two very sharp and intensively stained bands of 70 kb and 144 kb, respectively, possibly representing amplified rDNA and its dimer, contained more than ten times the nmber of DNAmolecules of most of the other bands. A to-1 of about 125 different subchromosomal elements have thus been identified in the macronucleus of Tetrahymena pyriformis. The sum of the DNAof all these individual molecules amounted to about 90 Mb, that is about 43 per cent of the haploid genomeof the micronuclei of this organism. (1) G. Chu, D. Vollrath, R.W.Davis, Science 234, P6
1582-1585
P8
(1986).
NUCLEAR DNA CHANGES WITHIN HELIANTHDs ANNUUS L.
Andrea Cavallini. Lucia Natali, Oria Sassoli, Pier Giorgio Cionini*, Mauro Durante. Dept. Agric.Plant Biol.,University of Pisa and *Dept. Plant Biology, University of Perugia, Italy. In Helianthus annuus, the amount of nuclear DNA continuously varied during reproduction, while the chromosome number and gross structure keep unchanged. Indeed, the results of cytophotometric determinations indicate that the DNA contents differ significantly even in seedlings obtained from seeds collected from different portions of single inflore scences of plants belonging to a selfed line. The variations take place during early embryo development, according to precise mechanisms controlling the variation range, and DNA sequences contained in the heterochromatin are mainly involved. The DNAs extracted from seedlings obtained from seeds collected at peripheral or central portions of an inflorescence were compared. The redundancy of sequences reassociating within C.t values (0.22. 0.22-2.1 and 2.1-100 is higher in the former than in the latter (1.72~105, 5.81.103, 5.26.102 vs. 1.23.105,4.30.103 respectively). These results are conand 2.46.lOi. firmed by slot blottings performed with the same DNA fractions. Moreover, these fractions isolated from the two DNAs show different restriction patterns. The observed nuclear DNA changes are correlated with some growth and phenotypic characters of the plants.